Restriction map for
pF1KSDB0025
(Circular) (Six-base) MAPSORT of: pF1KSDB0025.seq Check: 3833 from: 1 to: 4829
pF1KSDB0025 4829 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 225 225
Size: 4829
AatII G_ACGT'C
Cuts at: 209 209
Size: 4829
AccI GT'mk_AC
Cuts at: 209 1538 1867 209
Size: 1329 329 3171
Fragments arranged by size:
3171 1329 329
Acc65I G'GTAC_C
Cuts at: 1845 1845
Size: 4829
AclI AA'CG_TT
Cuts at: 4669 4669
Size: 4829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 1707 1754 2654 3086 3833 4101 824
Size: 883 47 900 432 747 268 1552
Fragments arranged by size:
1552 900 883 747 432 268 47
AflIII A'CryG_T
Cuts at: 866 2181 3285 4262 866
Size: 1315 1104 977 1433
Fragments arranged by size:
1433 1315 1104 977
AgeI A'CCGG_T
Cuts at: 3217 3217
Size: 4829
AhdI GACnn_n'nnGTC
Cuts at: 845 845
Size: 4829
AleI CACnn'nnGTG
Cuts at: 358 358
Size: 4829
AlwNI CAG_nnn'CTG
Cuts at: 1534 2134 3701 1534
Size: 600 1567 2662
Fragments arranged by size:
2662 1567 600
ApaLI G'TGCA_C
Cuts at: 1624 3599 1624
Size: 1975 2854
ApoI r'AATT_y
Cuts at: 1833 4182 4438 1833
Size: 2349 256 2224
Fragments arranged by size:
2349 2224 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4829
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
AvaI C'yCGr_G
Cuts at: 1849 1849
Size: 4829
BamHI G'GATC_C
Cuts at: 152 1778 1854 152
Size: 1626 76 3127
Fragments arranged by size:
3127 1626 76
BanI G'GyrC_C
Cuts at: 403 537 1014 1495 1845 2259 2507 2542 403
Size: 134 477 481 350 414 248 35 2690
Fragments arranged by size:
2690 481 477 414 350 248 134 35
BanII G_rGCy'C
Cuts at: 251 1843 2873 251
Size: 1592 1030 2207
Fragments arranged by size:
2207 1592 1030
BbeI G_GCGC'C
Cuts at: 2263 2511 2263
Size: 248 4581
BbsI GAAGACnn'nnnn_
Cuts at: 1148 1470 1570 1148
Size: 322 100 4407
Fragments arranged by size:
4407 322 100
BciVI GTATCCnnnnn_n'
Cuts at: 1298 2727 3488 4829 1298
Size: 1429 761 1341 1298
Fragments arranged by size:
1429 1341 1298 761
BclI T'GATC_A
Cuts at: 413 2349 413
Size: 1936 2893
BglI GCCn_nnn'nGGC
Cuts at: 1733 1733
Size: 4829
BglII A'GATC_T
Cuts at: 2344 4381 2344
Size: 2037 2792
BlpI GC'TnA_GC
Cuts at: 1271 1941 1271
Size: 670 4159
Bme1580I G_kGCm'C
Cuts at: 540 1498 1628 2454 2547 3603 540
Size: 958 130 826 93 1056 1766
Fragments arranged by size:
1766 1056 958 826 130 93
BmgBI CAC'GTC
Cuts at: 426 426
Size: 4829
BmrI ACTGGGnnnn_n'
Cuts at: 2041 2122 2457 4601 2041
Size: 81 335 2144 2269
Fragments arranged by size:
2269 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 4829
Bpu10I CC'TnA_GC
Cuts at: 731 731
Size: 4829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2005 2884 3376 3674 2005
Size: 879 492 298 3160
Fragments arranged by size:
3160 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 436 928 4533 436
Size: 492 3605 732
Fragments arranged by size:
3605 732 492
BsaAI yAC'GTr
Cuts at: 356 519 1821 2182 356
Size: 163 1302 361 3003
Fragments arranged by size:
3003 1302 361 163
BsaBI GATnn'nnATC
Cuts at: 6 157 2343 4380 6
Size: 151 2186 2037 455
Fragments arranged by size:
2186 2037 455 151
BsaHI Gr'CG_yC
Cuts at: 206 1475 2260 2508 3210 4706 206
Size: 1269 785 248 702 1496 329
Fragments arranged by size:
1496 1269 785 702 329 248
BsaWI w'CCGG_w
Cuts at: 2018 2539 3217 3491 3638 4049 2018
Size: 521 678 274 147 411 2798
Fragments arranged by size:
2798 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 158 188 1002 1032 158
Size: 30 814 30 3955
Fragments arranged by size:
3955 814 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 1223 1403 1595 1778 805
Size: 418 180 192 183 3856
Fragments arranged by size:
3856 418 192 183 180
BseYI C'CCAG_C
Cuts at: 2254 3012 3589 4085 2254
Size: 758 577 496 2998
Fragments arranged by size:
2998 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 929 1198 174
Size: 755 269 3805
Fragments arranged by size:
3805 755 269
BsiEI CG_ry'CG
Cuts at: 104 2371 2417 3625 104
Size: 2267 46 1208 1308
Fragments arranged by size:
2267 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 251 1628 1843 2621 2811 3603 251
Size: 1377 215 778 190 792 1477
Fragments arranged by size:
1477 1377 792 778 215 190
BsmI GAATG_Cn'
Cuts at: 354 354
Size: 4829
Bsp1286I G_dGCh'C
Cuts at: 251 540 1498 1628 1843 2454 2547 2621 2811
Size: 289 958 130 215 611 93 74 190
Cuts at: 2811 2873 3603 251
Size: 62 730 1477
Fragments arranged by size:
1477 958 730 611 289 215 190 130
93 74 62
BspEI T'CCGG_A
Cuts at: 4049 4049
Size: 4829
BspHI T'CATG_A
Cuts at: 251 344 605 1055 4005 251
Size: 93 261 450 2950 1075
Fragments arranged by size:
2950 1075 450 261 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 225 1368 1879 2093 2395 2776 225
Size: 1143 511 214 302 381 2278
Fragments arranged by size:
2278 1143 511 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3121 3175 4659 4824 50
Size: 3071 54 1484 165 55
Fragments arranged by size:
3071 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 827 2741 827
Size: 1914 2915
BsrFI r'CCGG_y
Cuts at: 1103 1498 2827 3008 3217 1103
Size: 395 1329 181 209 2715
Fragments arranged by size:
2715 1329 395 209 181
BsrGI T'GTAC_A
Cuts at: 623 623
Size: 4829
BssSI C'ACGA_G
Cuts at: 196 3100 3458 196
Size: 2904 358 1567
Fragments arranged by size:
2904 1567 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2206 2206
Size: 4829
BstBI TT'CG_AA
Cuts at: 110 3190 110
Size: 3080 1749
BstEII G'GTnAC_C
Cuts at: 1437 1437
Size: 4829
BstXI CCAn_nnnn'nTGG
Cuts at: 360 360
Size: 4829
BstYI r'GATC_y
Cuts at: 1 152 1154 1778 1854 2321 2344 2679 2925
Size: 151 1002 624 76 467 23 335 246
Cuts at: 2925 3937 4023 4035 4381 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 1002 624 467 449 346 335 246
151 86 76 23 12
Bsu36I CC'TnA_GG
Cuts at: 1516 1516
Size: 4829
BtgI C'CryG_G
Cuts at: 375 1422 375
Size: 1047 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2779 2960 3123 2779
Size: 181 163 4485
Fragments arranged by size:
4485 181 163
BtsI GCAGTG_nn'
Cuts at: 941 2072 941
Size: 1131 3698
ClaI AT'CG_AT
Cuts at: 1745 1745
Size: 4829
DraI TTT'AAA
Cuts at: 1571 1828 1571
Size: 257 4572
DraIII CAC_nnn'GTG
Cuts at: 533 1428 533
Size: 895 3934
DrdI GACnn_nn'nnGTC
Cuts at: 493 2535 3393 493
Size: 2042 858 1929
Fragments arranged by size:
2042 1929 858
EaeI y'GGCC_r
Cuts at: 420 482 719 1067 1247 2414 2588 2979 3006
Size: 62 237 348 180 1167 174 391 27
Cuts at: 3006 4351 420
Size: 1345 898
Fragments arranged by size:
1345 1167 898 391 348 237 180 174
62 27
EagI C'GGCC_G
Cuts at: 2414 2414
Size: 4829
EarI CTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 946 3037 3347 3493 4639 946
Size: 2091 310 146 1146 1136
Fragments arranged by size:
2091 1146 1136 310 146
EcoICRI GAG'CTC
Cuts at: 249 1841 249
Size: 1592 3237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 1707 1754 2654 3086 3833 4101 824
Size: 883 47 900 432 747 268 1552
Fragments arranged by size:
1552 900 883 747 432 268 47
EcoNI CCTnn'n_nnAGG
Cuts at: 160 1793 160
Size: 1633 3196
EcoO109I rG'GnC_Cy
Cuts at: 618 1341 1520 1704 1968 618
Size: 723 179 184 264 3479
Fragments arranged by size:
3479 723 264 184 179
EcoRI G'AATT_C
Cuts at: 1833 1833
Size: 4829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1161 2848 1161
Size: 1687 3142
FspI TGC'GCA
Cuts at: 2610 2610
Size: 4829
HaeII r_GCGC'y
Cuts at: 2263 2511 3533 4512 2263
Size: 248 1022 979 2580
Fragments arranged by size:
2580 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 768 800 831 863 1770 1802 2671 2701 2703
Size: 32 31 32 907 32 869 30 2
Cuts at: 2703 2733 768
Size: 30 2864
Fragments arranged by size:
2864 907 869 32 32 32 31 30
30 2
HincII GTy'rAC
Cuts at: 588 864 1868 588
Size: 276 1004 3549
Fragments arranged by size:
3549 1004 276
HindIII A'AGCT_T
Cuts at: 1273 1273
Size: 4829
KasI G'GCGC_C
Cuts at: 2259 2507 2259
Size: 248 4581
KpnI G_GTAC'C
Cuts at: 1849 1849
Size: 4829
MluI A'CGCG_T
Cuts at: 4262 4262
Size: 4829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 378 1197 1286 3500 3684 378
Size: 819 89 2214 184 1523
Fragments arranged by size:
2214 1523 819 184 89
MscI TGG'CCA
Cuts at: 1069 2590 1069
Size: 1521 3308
MslI CAynn'nnrTG
Cuts at: 358 409 2818 2945 358
Size: 51 2409 127 2242
Fragments arranged by size:
2409 2242 127 51
MspA1I CmG'CkG
Cuts at: 901 1940 2254 2614 3627 3872 901
Size: 1039 314 360 1013 245 1858
Fragments arranged by size:
1858 1039 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3010 3010
Size: 4829
NarI GG'CG_CC
Cuts at: 2260 2508 2260
Size: 248 4581
NcoI C'CATG_G
Cuts at: 375 375
Size: 4829
NgoMIV G'CCGG_C
Cuts at: 3008 3008
Size: 4829
NspI r_CATG'y
Cuts at: 630 952 1416 1882 2382 3289 630
Size: 322 464 466 500 907 2170
Fragments arranged by size:
2170 907 500 466 464 322
PasI CC'CwG_GG
Cuts at: 1630 1630
Size: 4829
PciI A'CATG_T
Cuts at: 3285 3285
Size: 4829
PflMI CCAn_nnn'nTGG
Cuts at: 237 237
Size: 4829
PfoI T'CCnGG_A
Cuts at: 1311 1791 1311
Size: 480 4349
PmeI GTTT'AAAC
Cuts at: 1828 1828
Size: 4829
PmlI CAC'GTG
Cuts at: 356 519 356
Size: 163 4666
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4797
PpuMI rG'GwC_Cy
Cuts at: 618 1341 1520 1704 618
Size: 723 179 184 3743
Fragments arranged by size:
3743 723 184 179
PstI C_TGCA'G
Cuts at: 1876 2561 1876
Size: 685 4144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4829
PvuII CAG'CTG
Cuts at: 901 2254 2614 901
Size: 1353 360 3116
Fragments arranged by size:
3116 1353 360
RsrII CG'GwC_CG
Cuts at: 3024 3024
Size: 4829
SacI G_AGCT'C
Cuts at: 251 1843 251
Size: 1592 3237
SalI G'TCGA_C
Cuts at: 1866 1866
Size: 4829
SanDI GG'GwC_CC
Cuts at: 1341 1341
Size: 4829
SapI GCTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
SbfI CC_TGCA'GG
Cuts at: 1876 1876
Size: 4829
ScaI AGT'ACT
Cuts at: 4346 4346
Size: 4829
SexAI A'CCwGG_T
Cuts at: 529 857 529
Size: 328 4501
SfcI C'TryA_G
Cuts at: 33 1872 2557 3550 3741 33
Size: 1839 685 993 191 1121
Fragments arranged by size:
1839 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2261 2509 2261
Size: 248 4581
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
SmaI CCC'GGG
Cuts at: 1851 1851
Size: 4829
SmlI C'TyrA_G
Cuts at: 1984 2899 3391 3653 1984
Size: 915 492 262 3160
Fragments arranged by size:
3160 915 492 262
SnaBI TAC'GTA
Cuts at: 1821 1821
Size: 4829
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4829
SphI G_CATG'C
Cuts at: 1882 2382 1882
Size: 500 4329
StyI C'CwwG_G
Cuts at: 269 375 383 491 542 1963 2317 269
Size: 106 8 108 51 1421 354 2781
Fragments arranged by size:
2781 1421 354 108 106 51 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 208 340 555 2412 2545 3004 3213 208
Size: 132 215 1857 133 459 209 1824
Fragments arranged by size:
1857 1824 459 215 209 133 132
TatI w'GTAC_w
Cuts at: 623 4344 623
Size: 3721 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 158 190 281 313 1002 1034 4346 4378 158
Size: 32 91 32 689 32 3312 32 609
Fragments arranged by size:
3312 689 609 91 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1534 2626 1534
Size: 1092 3737
XbaI T'CTAG_A
Cuts at: 1860 1860
Size: 4829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 291 703 2324 291
Size: 412 1621 2796
Fragments arranged by size:
2796 1621 412
XmaI C'CCGG_G
Cuts at: 1849 1849
Size: 4829
ZraI GAC'GTC
Cuts at: 207 207
Size: 4829
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BpmI BsiWI BsmBI BssHII
BstZ17I CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI