Restriction map for
pF1KSDB0018
(Circular) (Six-base) MAPSORT of: pF1KSDB0018.seq Check: 290 from: 1 to: 5777
pF1KSDB0018 5777 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 285 384 285
Size: 99 5678
AccI GT'mk_AC
Cuts at: 2030 2765 2815 2030
Size: 735 50 4992
Fragments arranged by size:
4992 735 50
Acc65I G'GTAC_C
Cuts at: 442 2793 442
Size: 2351 3426
AclI AA'CG_TT
Cuts at: 335 5617 335
Size: 5282 495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1094 2467 2609 3602 4034 4781 5049 1000
Size: 94 1373 142 993 432 747 268 1728
Fragments arranged by size:
1728 1373 993 747 432 268 142 94
AflIII A'CryG_T
Cuts at: 885 3129 4233 5210 885
Size: 2244 1104 977 1452
Fragments arranged by size:
2244 1452 1104 977
AgeI A'CCGG_T
Cuts at: 4165 4165
Size: 5777
AleI CACnn'nnGTG
Cuts at: 628 628
Size: 5777
AlwNI CAG_nnn'CTG
Cuts at: 216 300 1594 1815 3082 4649 216
Size: 84 1294 221 1267 1567 1344
Fragments arranged by size:
1567 1344 1294 1267 221 84
ApaI G_GGCC'C
Cuts at: 2234 2234
Size: 5777
ApaLI G'TGCA_C
Cuts at: 4547 4547
Size: 5777
ApoI r'AATT_y
Cuts at: 2781 5130 5386 2781
Size: 2349 256 3172
Fragments arranged by size:
3172 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5777
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
AvaI C'yCGr_G
Cuts at: 404 2609 2797 404
Size: 2205 188 3384
Fragments arranged by size:
3384 2205 188
AvrII C'CTAG_G
Cuts at: 165 165
Size: 5777
BamHI G'GATC_C
Cuts at: 860 2802 860
Size: 1942 3835
BanI G'GyrC_C
Cuts at: 372 442 618 716 1197 1211 2263 2793 3207
Size: 70 176 98 481 14 1052 530 414
Cuts at: 3207 3455 3490 372
Size: 248 35 2659
Fragments arranged by size:
2659 1052 530 481 414 248 176 98
70 35 14
BanII G_rGCy'C
Cuts at: 1075 2234 2791 3821 1075
Size: 1159 557 1030 3031
Fragments arranged by size:
3031 1159 1030 557
BbeI G_GCGC'C
Cuts at: 622 720 3211 3459 622
Size: 98 2491 248 2940
Fragments arranged by size:
2940 2491 248 98
BbsI GAAGACnn'nnnn_
Cuts at: 239 1102 2523 239
Size: 863 1421 3493
Fragments arranged by size:
3493 1421 863
BbvCI CC'TCA_GC
Cuts at: 368 1123 368
Size: 755 5022
BciVI GTATCCnnnnn_n'
Cuts at: 196 3675 4436 5777 196
Size: 3479 761 1341 196
Fragments arranged by size:
3479 1341 761 196
BclI T'GATC_A
Cuts at: 3297 3297
Size: 5777
BglI GCCn_nnn'nGGC
Cuts at: 364 714 1456 2146 364
Size: 350 742 690 3995
Fragments arranged by size:
3995 742 690 350
BglII A'GATC_T
Cuts at: 3292 5329 3292
Size: 2037 3740
BlpI GC'TnA_GC
Cuts at: 2889 2889
Size: 5777
Bme1580I G_kGCm'C
Cuts at: 784 1999 2234 2266 3402 3495 4551 784
Size: 1215 235 32 1136 93 1056 2010
Fragments arranged by size:
2010 1215 1136 1056 235 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 778 919 1272 2989 3070 3405 5549 778
Size: 141 353 1717 81 335 2144 1006
Fragments arranged by size:
2144 1717 1006 353 335 141 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 5777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 601 658 1170 2684 238
Size: 363 57 512 1514 3331
Fragments arranged by size:
3331 1514 512 363 57
Bpu10I CC'TnA_GC
Cuts at: 368 1123 2060 2102 2168 368
Size: 755 937 42 66 3977
Fragments arranged by size:
3977 937 755 66 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2953 3832 4324 4622 2953
Size: 879 492 298 4108
Fragments arranged by size:
4108 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 420 1229 1298 1718 2448 2471 5481 420
Size: 809 69 420 730 23 3010 716
Fragments arranged by size:
3010 809 730 716 420 69 23
BsaAI yAC'GTr
Cuts at: 779 886 2769 3130 779
Size: 107 1883 361 3426
Fragments arranged by size:
3426 1883 361 107
BsaBI GATnn'nnATC
Cuts at: 6 502 1636 3291 5328 6
Size: 496 1134 1655 2037 455
Fragments arranged by size:
2037 1655 1134 496 455
BsaHI Gr'CG_yC
Cuts at: 619 717 1418 3208 3456 4158 5654 619
Size: 98 701 1790 248 702 1496 742
Fragments arranged by size:
1790 1496 742 702 701 248 98
BsaWI w'CCGG_w
Cuts at: 857 866 2411 2966 3487 4165 4439 4586 4997
Size: 9 1545 555 521 678 274 147 411
Cuts at: 4997 857
Size: 1637
Fragments arranged by size:
1637 1545 678 555 521 411 274 147
9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1427 1457 1576 1606 1867 1897 1971 2001 2612
Size: 30 119 30 261 30 74 30 611
Cuts at: 2612 2642 1427
Size: 30 4562
Fragments arranged by size:
4562 611 261 119 74 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 655 658 667 670 1351 1957 2650 2653 2656
Size: 3 9 3 681 606 693 3 3
Cuts at: 2656 2671 2764 2767 655
Size: 15 93 3 3665
Fragments arranged by size:
3665 693 681 606 93 15 9 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 131 434 585 899 1040 1255 1270 1406 1515
Size: 303 151 314 141 215 15 136 109
Cuts at: 1515 1762 2047 2583 3202 3960 4537 5033 131
Size: 247 285 536 619 758 577 496 875
Fragments arranged by size:
875 758 619 577 536 496 314 303
285 247 215 151 141 136 109 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 652 759 775 808 930 1021 201
Size: 451 107 16 33 122 91 4957
Fragments arranged by size:
4957 451 122 107 91 33 16
BsiEI CG_ry'CG
Cuts at: 104 3319 3365 4573 104
Size: 3215 46 1208 1308
Fragments arranged by size:
3215 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2791 3569 3759 4551 2791
Size: 778 190 792 4017
Fragments arranged by size:
4017 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 520 520
Size: 5777
Bsp1286I G_dGCh'C
Cuts at: 784 1075 1999 2234 2266 2791 3402 3495 3569
Size: 291 924 235 32 525 611 93 74
Cuts at: 3569 3759 3821 4551 784
Size: 190 62 730 2010
Fragments arranged by size:
2010 924 730 611 525 291 235 190
93 74 62 32
BspEI T'CCGG_A
Cuts at: 857 866 2411 4997 857
Size: 9 1545 2586 1637
Fragments arranged by size:
2586 1637 1545 9
BspHI T'CATG_A
Cuts at: 1389 4953 1389
Size: 3564 2213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 285 384 2827 3041 3343 3724 285
Size: 99 2443 214 302 381 2338
Fragments arranged by size:
2443 2338 381 302 214 99
BsrBI CCG'CTC
Cuts at: 50 1064 2674 4069 4123 5607 5772 50
Size: 1014 1610 1395 54 1484 165 55
Fragments arranged by size:
1610 1484 1395 1014 165 55 54
BsrDI GCAATG_nn'
Cuts at: 429 3689 429
Size: 3260 2517
BsrFI r'CCGG_y
Cuts at: 3775 3956 4165 3775
Size: 181 209 5387
Fragments arranged by size:
5387 209 181
BssSI C'ACGA_G
Cuts at: 628 4048 4406 628
Size: 3420 358 1999
Fragments arranged by size:
3420 1999 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3154 3154
Size: 5777
BstBI TT'CG_AA
Cuts at: 110 4138 110
Size: 4028 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1194 2520 1194
Size: 1326 4451
BstYI r'GATC_y
Cuts at: 1 860 1631 2802 3269 3292 3627 3873 4885
Size: 859 771 1171 467 23 335 246 1012
Cuts at: 4885 4971 4983 5329 1
Size: 86 12 346 449
Fragments arranged by size:
1171 1012 859 771 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1207 1534 2288 2747 1207
Size: 327 754 459 4237
Fragments arranged by size:
4237 754 459 327
BtgI C'CryG_G
Cuts at: 797 797
Size: 5777
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3727 3908 4071 3727
Size: 181 163 5433
Fragments arranged by size:
5433 181 163
BtsI GCAGTG_nn'
Cuts at: 771 2028 2059 3020 771
Size: 1257 31 961 3528
Fragments arranged by size:
3528 1257 961 31
DraI TTT'AAA
Cuts at: 2776 2776
Size: 5777
DrdI GACnn_nn'nnGTC
Cuts at: 1825 3483 4341 1825
Size: 1658 858 3261
Fragments arranged by size:
3261 1658 858
EaeI y'GGCC_r
Cuts at: 3362 3536 3927 3954 5299 3362
Size: 174 391 27 1345 3840
Fragments arranged by size:
3840 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3362 3362
Size: 5777
EarI CTCTTCn'nnn_
Cuts at: 1113 1934 3800 4010 1113
Size: 821 1866 210 2880
Fragments arranged by size:
2880 1866 821 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3985 4295 4441 5587 3985
Size: 310 146 1146 4175
Fragments arranged by size:
4175 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2789 2789
Size: 5777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 601 658 1000 1094 1170 2467 2609 2684
Size: 363 57 342 94 76 1297 142 75
Cuts at: 2684 3602 4034 4781 5049 238
Size: 918 432 747 268 966
Fragments arranged by size:
1297 966 918 747 432 363 342 268
142 94 76 75 57
EcoNI CCTnn'n_nnAGG
Cuts at: 140 367 1616 1939 2291 140
Size: 227 1249 323 352 3626
Fragments arranged by size:
3626 1249 352 323 227
EcoO109I rG'GnC_Cy
Cuts at: 161 1127 1181 1854 2071 2108 2280 2337 2427
Size: 966 54 673 217 37 172 57 90
Cuts at: 2427 2706 2916 161
Size: 279 210 3022
Fragments arranged by size:
3022 966 673 279 217 210 172 90
57 54 37
EcoRI G'AATT_C
Cuts at: 2781 2781
Size: 5777
EcoRV GAT'ATC
Cuts at: 1475 1475
Size: 5777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 3796 2755
Size: 1041 4736
FspI TGC'GCA
Cuts at: 547 3558 547
Size: 3011 2766
HaeII r_GCGC'y
Cuts at: 606 622 709 720 3211 3459 4481 5460 606
Size: 16 87 11 2491 248 1022 979 923
Fragments arranged by size:
2491 1022 979 923 248 87 16 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1015 1047 1576 1608 1715 1747 1971
Size: 32 532 32 529 32 107 32 224
Cuts at: 1971 2003 2350 2382 2740 2772 3619 3649 3651
Size: 32 347 32 358 32 847 30 2
Cuts at: 3651 3681 451
Size: 30 2547
Fragments arranged by size:
2547 847 532 529 358 347 224 107
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2816 2816
Size: 5777
KasI G'GCGC_C
Cuts at: 618 716 3207 3455 618
Size: 98 2491 248 2940
Fragments arranged by size:
2940 2491 248 98
KpnI G_GTAC'C
Cuts at: 446 2797 446
Size: 2351 3426
MluI A'CGCG_T
Cuts at: 5210 5210
Size: 5777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 863 1630 4448 4632 863
Size: 767 2818 184 2008
Fragments arranged by size:
2818 2008 767 184
MscI TGG'CCA
Cuts at: 3538 3538
Size: 5777
MslI CAynn'nnrTG
Cuts at: 628 2269 3766 3893 628
Size: 1641 1497 127 2512
Fragments arranged by size:
2512 1641 1497 127
MspA1I CmG'CkG
Cuts at: 636 944 1255 1406 1815 2176 2888 3202 3562
Size: 308 311 151 409 361 712 314 360
Cuts at: 3562 4575 4820 636
Size: 1013 245 1593
Fragments arranged by size:
1593 1013 712 409 361 360 314 311
308 245 151
NaeI GCC'GGC
Cuts at: 3958 3958
Size: 5777
NarI GG'CG_CC
Cuts at: 619 717 3208 3456 619
Size: 98 2491 248 2940
Fragments arranged by size:
2940 2491 248 98
NcoI C'CATG_G
Cuts at: 797 797
Size: 5777
NgoMIV G'CCGG_C
Cuts at: 3956 3956
Size: 5777
NruI TCG'CGA
Cuts at: 254 254
Size: 5777
NspI r_CATG'y
Cuts at: 2387 2830 3330 4237 2387
Size: 443 500 907 3927
Fragments arranged by size:
3927 907 500 443
PasI CC'CwG_GG
Cuts at: 1361 1361
Size: 5777
PciI A'CATG_T
Cuts at: 4233 4233
Size: 5777
PflMI CCAn_nnn'nTGG
Cuts at: 879 921 1382 1815 1830 2097 879
Size: 42 461 433 15 267 4559
Fragments arranged by size:
4559 461 433 267 42 15
PfoI T'CCnGG_A
Cuts at: 1547 1547
Size: 5777
PmeI GTTT'AAAC
Cuts at: 2776 2776
Size: 5777
PmlI CAC'GTG
Cuts at: 779 779
Size: 5777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 4948 4916
Size: 32 5745
PpuMI rG'GwC_Cy
Cuts at: 161 1854 2071 2108 2280 2427 161
Size: 1693 217 37 172 147 3511
Fragments arranged by size:
3511 1693 217 172 147 37
PspOMI G'GGCC_C
Cuts at: 2230 2230
Size: 5777
PstI C_TGCA'G
Cuts at: 296 2824 3509 296
Size: 2528 685 2564
Fragments arranged by size:
2564 2528 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5777
PvuII CAG'CTG
Cuts at: 636 1255 1406 1815 2176 3202 3562 636
Size: 619 151 409 361 1026 360 2851
Fragments arranged by size:
2851 1026 619 409 361 360 151
RsrII CG'GwC_CG
Cuts at: 3972 3972
Size: 5777
SacI G_AGCT'C
Cuts at: 2791 2791
Size: 5777
SalI G'TCGA_C
Cuts at: 2814 2814
Size: 5777
SanDI GG'GwC_CC
Cuts at: 2108 2427 2108
Size: 319 5458
SapI GCTCTTCn'nnn_
Cuts at: 3800 4010 3800
Size: 210 5567
SbfI CC_TGCA'GG
Cuts at: 296 2824 296
Size: 2528 3249
ScaI AGT'ACT
Cuts at: 5294 5294
Size: 5777
SfcI C'TryA_G
Cuts at: 33 292 1864 1929 2820 3505 4498 4689 33
Size: 259 1572 65 891 685 993 191 1121
Fragments arranged by size:
1572 1121 993 891 685 259 191 65
SfoI GGC'GCC
Cuts at: 620 718 3209 3457 620
Size: 98 2491 248 2940
Fragments arranged by size:
2940 2491 248 98
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
SmaI CCC'GGG
Cuts at: 406 2611 2799 406
Size: 2205 188 3384
Fragments arranged by size:
3384 2205 188
SmlI C'TyrA_G
Cuts at: 2932 3847 4339 4601 2932
Size: 915 492 262 4108
Fragments arranged by size:
4108 915 492 262
SnaBI TAC'GTA
Cuts at: 2769 2769
Size: 5777
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5777
SphI G_CATG'C
Cuts at: 2830 3330 2830
Size: 500 5277
StuI AGG'CCT
Cuts at: 1622 2487 1622
Size: 865 4912
StyI C'CwwG_G
Cuts at: 165 797 1559 1617 1898 2258 2354 2378 2558
Size: 632 762 58 281 360 96 24 180
Cuts at: 2558 2911 3265 165
Size: 353 354 2677
Fragments arranged by size:
2677 762 632 360 354 353 281 180
96 58 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 820 1130 1284 2281 3360 3493 3952 4161 820
Size: 310 154 997 1079 133 459 209 2436
Fragments arranged by size:
2436 1079 997 459 310 209 154 133
TatI w'GTAC_w
Cuts at: 5292 5292
Size: 5777
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 810 842 1427 1459 5294 5326 810
Size: 32 585 32 3835 32 1261
Fragments arranged by size:
3835 1261 585 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 463 3574 463
Size: 3111 2666
XbaI T'CTAG_A
Cuts at: 2808 2808
Size: 5777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2094 3272 2094
Size: 1178 4599
XmaI C'CCGG_G
Cuts at: 404 2609 2797 404
Size: 2205 188 3384
Fragments arranged by size:
3384 2205 188
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI