Restriction map for
pF1KSDB0004
(Circular) (Six-base) MAPSORT of: pF1KSDB0004.seq Check: 6381 from: 1 to: 6397
pF1KSDB0004 6397 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2408 2666 2408
Size: 258 6139
AatII G_ACGT'C
Cuts at: 2777 2777
Size: 6397
AccI GT'mk_AC
Cuts at: 3433 3433
Size: 6397
Acc65I G'GTAC_C
Cuts at: 3411 3411
Size: 6397
AclI AA'CG_TT
Cuts at: 6237 6237
Size: 6397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 1686 4220 4652 5401 5669 804
Size: 882 2534 432 749 268 1532
Fragments arranged by size:
2534 1532 882 749 432 268
AfeI AGC'GCT
Cuts at: 2576 2576
Size: 6397
AflIII A'CryG_T
Cuts at: 3747 4853 5830 3747
Size: 1106 977 4314
Fragments arranged by size:
4314 1106 977
AgeI A'CCGG_T
Cuts at: 897 4785 897
Size: 3888 2509
AleI CACnn'nnGTG
Cuts at: 598 2402 598
Size: 1804 4593
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 2176
Size: 32 6365
AlwNI CAG_nnn'CTG
Cuts at: 611 1298 1310 1446 1671 1767 3216 3308 3700
Size: 687 12 136 225 96 1449 92 392
Cuts at: 3700 5269 611
Size: 1569 1739
Fragments arranged by size:
1739 1569 1449 687 392 225 136 96
92 12
ApaI G_GGCC'C
Cuts at: 631 784 1616 3374 631
Size: 153 832 1758 3654
Fragments arranged by size:
3654 1758 832 153
ApaLI G'TGCA_C
Cuts at: 1170 2500 5167 1170
Size: 1330 2667 2400
Fragments arranged by size:
2667 2400 1330
ApoI r'AATT_y
Cuts at: 3399 5750 6006 3399
Size: 2351 256 3790
Fragments arranged by size:
3790 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6397
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6397
AvaI C'yCGr_G
Cuts at: 739 957 1074 1354 1362 1609 1615 2886 2934
Size: 218 117 280 8 247 6 1271 48
Cuts at: 2934 3415 739
Size: 481 3721
Fragments arranged by size:
3721 1271 481 280 247 218 117 48
8 6
BamHI G'GATC_C
Cuts at: 653 3420 653
Size: 2767 3630
BanI G'GyrC_C
Cuts at: 748 900 1042 1867 2083 2203 2311 2396 2845
Size: 152 142 825 216 120 108 85 449
Cuts at: 2845 3411 3825 4073 4108 748
Size: 566 414 248 35 3037
Fragments arranged by size:
3037 825 566 449 414 248 216 152
142 120 108 85 35
BanII G_rGCy'C
Cuts at: 416 479 554 631 784 948 958 1256 1616
Size: 63 75 77 153 164 10 298 360
Cuts at: 1616 2141 2414 2957 3080 3374 3409 4439 416
Size: 525 273 543 123 294 35 1030 2374
Fragments arranged by size:
2374 1030 543 525 360 298 294 273
164 153 123 77 75 63 35 10
BbeI G_GCGC'C
Cuts at: 1871 2087 3829 4077 1871
Size: 216 1742 248 4191
Fragments arranged by size:
4191 1742 248 216
BbsI GAAGACnn'nnnn_
Cuts at: 1692 2433 1692
Size: 741 5656
BbvCI CC'TCA_GC
Cuts at: 1439 2713 1439
Size: 1274 5123
BciVI GTATCCnnnnn_n'
Cuts at: 4293 5056 6397 4293
Size: 763 1341 4293
Fragments arranged by size:
4293 1341 763
BclI T'GATC_A
Cuts at: 3915 3915
Size: 6397
BglI GCCn_nnn'nGGC
Cuts at: 1301 1336 1479 2388 2860 3038 1301
Size: 35 143 909 472 178 4660
Fragments arranged by size:
4660 909 472 178 143 35
BglII A'GATC_T
Cuts at: 3910 5949 3910
Size: 2039 4358
BlpI GC'TnA_GC
Cuts at: 247 2113 3507 247
Size: 1866 1394 3137
Fragments arranged by size:
3137 1866 1394
Bme1580I G_kGCm'C
Cuts at: 631 784 1174 1616 2314 2399 2504 2848 3374
Size: 153 390 442 698 85 105 344 526
Cuts at: 3374 4020 4113 5171 631
Size: 646 93 1058 1857
Fragments arranged by size:
1857 1058 698 646 526 442 390 344
153 105 93 85
BmgBI CAC'GTC
Cuts at: 708 708
Size: 6397
BmrI ACTGGGnnnn_n'
Cuts at: 2835 3607 3688 4023 6169 2835
Size: 772 81 335 2146 3063
Fragments arranged by size:
3063 2146 772 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2613 4762 2613
Size: 2149 4248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1119 2026 2608 2623 2803 2850 1039
Size: 80 907 582 15 180 47 4586
Fragments arranged by size:
4586 907 582 180 80 47 15
Bpu10I CC'TnA_GC
Cuts at: 1439 1789 2713 1439
Size: 350 924 5123
Fragments arranged by size:
5123 924 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3571 4450 4944 5242 3571
Size: 879 494 298 4726
Fragments arranged by size:
4726 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6101 6101
Size: 6397
BsaAI yAC'GTr
Cuts at: 1500 1721 1901 2916 3123 3387 3748 1500
Size: 221 180 1015 207 264 361 4149
Fragments arranged by size:
4149 1015 361 264 221 207 180
BsaBI GATnn'nnATC
Cuts at: 6 3909 5948 6
Size: 3903 2039 455
Fragments arranged by size:
3903 2039 455
BsaHI Gr'CG_yC
Cuts at: 1868 2084 2438 2642 2774 2987 3826 4074 4778
Size: 216 354 204 132 213 839 248 704
Cuts at: 4778 6274 1868
Size: 1496 1991
Fragments arranged by size:
1991 1496 839 704 354 248 216 213
204 132
BsaWI w'CCGG_w
Cuts at: 897 3584 4105 4785 5059 5206 5617 897
Size: 2687 521 680 274 147 411 1677
Fragments arranged by size:
2687 1677 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 719 749 1094 1124 2176 2206 2464 2494 719
Size: 30 345 30 1052 30 258 30 4622
Fragments arranged by size:
4622 1052 345 258 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 523 526 535 544 547 550 1945 2149
Size: 252 3 9 9 3 3 1395 204
Cuts at: 2149 271
Size: 4519
Fragments arranged by size:
4519 1395 252 204 9 9 3 3
3
BseYI C'CCAG_C
Cuts at: 563 765 783 884 914 962 967 1291 1454
Size: 202 18 101 30 48 5 324 163
Cuts at: 1454 1770 2188 2542 2966 3230 3820 4578 5157
Size: 316 418 354 424 264 590 758 579
Cuts at: 5157 5653 563
Size: 496 1307
Fragments arranged by size:
1307 758 590 579 496 424 418 354
324 316 264 202 163 101 48 30
18 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 267 571 1213 1669 1880 2005 2548 3103
Size: 123 304 642 456 211 125 543 555
Cuts at: 3103 3120 3145 3187 144
Size: 17 25 42 3354
Fragments arranged by size:
3354 642 555 543 456 304 211 125
123 42 25 17
BsiEI CG_ry'CG
Cuts at: 104 137 179 698 1819 3379 3937 3983 5193
Size: 33 42 519 1121 1560 558 46 1210
Cuts at: 5193 104
Size: 1308
Fragments arranged by size:
1560 1308 1210 1121 558 519 46 42
33
BsiHKAI G_wGCw'C
Cuts at: 196 1174 1207 2414 2504 3409 4187 4377 5171
Size: 978 33 1207 90 905 778 190 794
Cuts at: 5171 196
Size: 1422
Fragments arranged by size:
1422 1207 978 905 794 778 190 90
33
Bsp1286I G_dGCh'C
Cuts at: 196 416 479 554 631 784 948 958 1174
Size: 220 63 75 77 153 164 10 216
Cuts at: 1174 1207 1256 1616 2141 2314 2399 2414 2504
Size: 33 49 360 525 173 85 15 90
Cuts at: 2504 2848 2957 3080 3374 3409 4020 4113 4187
Size: 344 109 123 294 35 611 93 74
Cuts at: 4187 4377 4439 5171 196
Size: 190 62 732 1422
Fragments arranged by size:
1422 732 611 525 360 344 294 220
216 190 173 164 153 123 109 93
90 85 77 75 74 63 62 49
35 33 15 10
BspEI T'CCGG_A
Cuts at: 5617 5617
Size: 6397
BspHI T'CATG_A
Cuts at: 5573 5573
Size: 6397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 430 2408 2666 3055 3125 3201 3445 3659 3961
Size: 1978 258 389 70 76 244 214 302
Cuts at: 3961 4342 430
Size: 381 2485
Fragments arranged by size:
2485 1978 389 381 302 258 244 214
76 70
BsrBI CCG'CTC
Cuts at: 50 544 1815 1833 2592 3021 3342 4687 4741
Size: 494 1271 18 759 429 321 1345 54
Cuts at: 4741 6227 6392 50
Size: 1486 165 55
Fragments arranged by size:
1486 1345 1271 759 494 429 321 165
55 54 18
BsrDI GCAATG_nn'
Cuts at: 4307 4307
Size: 6397
BsrFI r'CCGG_y
Cuts at: 178 697 897 931 1060 1945 2080 3247 3358
Size: 519 200 34 129 885 135 1167 111
Cuts at: 3358 4393 4574 4785 178
Size: 1035 181 211 1790
Fragments arranged by size:
1790 1167 1035 885 519 211 200 181
135 129 111 34
BsrGI T'GTAC_A
Cuts at: 2927 2927
Size: 6397
BssHII G'CGCG_C
Cuts at: 2555 2745 2555
Size: 190 6207
BssSI C'ACGA_G
Cuts at: 3025 4666 5026 3025
Size: 1641 360 4396
Fragments arranged by size:
4396 1641 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1310 1671 1767 3047 3772 1310
Size: 361 96 1280 725 3935
Fragments arranged by size:
3935 1280 725 361 96
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6397
BstXI CCAn_nnnn'nTGG
Cuts at: 1246 1914 1246
Size: 668 5729
BstYI r'GATC_y
Cuts at: 1 653 3420 3887 3910 4245 4491 5505 5591
Size: 652 2767 467 23 335 246 1014 86
Cuts at: 5591 5603 5949 1
Size: 12 346 449
Fragments arranged by size:
2767 1014 652 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 662 905 1477 662
Size: 243 572 5582
Fragments arranged by size:
5582 572 243
BtgI C'CryG_G
Cuts at: 125 131 470 599 649 1031 1634 1907 2338
Size: 6 339 129 50 382 603 273 431
Cuts at: 2338 2483 2561 2645 2957 3012 125
Size: 145 78 84 312 55 3510
Fragments arranged by size:
3510 603 431 382 339 312 273 145
129 84 78 55 50 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3262 4345 4526 4689 3262
Size: 1083 181 163 4970
Fragments arranged by size:
4970 1083 181 163
BtsI GCAGTG_nn'
Cuts at: 496 1086 1263 1402 1416 1672 1738 2436 3133
Size: 590 177 139 14 256 66 698 697
Cuts at: 3133 3638 496
Size: 505 3255
Fragments arranged by size:
3255 698 697 590 505 256 177 139
66 14
DraI TTT'AAA
Cuts at: 3394 3394
Size: 6397
DraIII CAC_nnn'GTG
Cuts at: 711 711
Size: 6397
DrdI GACnn_nn'nnGTC
Cuts at: 4101 4961 4101
Size: 860 5537
EaeI y'GGCC_r
Cuts at: 128 176 180 467 602 695 1097 1816 2012
Size: 48 4 287 135 93 402 719 196
Cuts at: 2012 2069 2380 2389 2549 2706 2981 3980 4154
Size: 57 311 9 160 157 275 999 174
Cuts at: 4154 4545 4572 5919 128
Size: 391 27 1347 606
Fragments arranged by size:
1347 999 719 606 402 391 311 287
275 196 174 160 157 135 93 57
48 27 9 4
EagI C'GGCC_G
Cuts at: 176 695 1816 3980 176
Size: 519 1121 2164 2593
Fragments arranged by size:
2593 2164 1121 519
EarI CTCTTCn'nnn_
Cuts at: 1019 1963 2620 4418 4628 1019
Size: 944 657 1798 210 2788
Fragments arranged by size:
2788 1798 944 657 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 562 1825 4603 4915 5061 6207 562
Size: 1263 2778 312 146 1146 752
Fragments arranged by size:
2778 1263 1146 752 312 146
EcoICRI GAG'CTC
Cuts at: 2412 3407 2412
Size: 995 5402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 1039 1119 1686 2026 2608 2623 2803 2850
Size: 235 80 567 340 582 15 180 47
Cuts at: 2850 4220 4652 5401 5669 804
Size: 1370 432 749 268 1532
Fragments arranged by size:
1532 1370 749 582 567 432 340 268
235 180 80 47 15
EcoNI CCTnn'n_nnAGG
Cuts at: 304 304
Size: 6397
EcoO109I rG'GnC_Cy
Cuts at: 381 627 666 780 810 971 2883 3213 3326
Size: 246 39 114 30 161 1912 330 113
Cuts at: 3326 3371 3534 381
Size: 45 163 3244
Fragments arranged by size:
3244 1912 330 246 163 161 114 113
45 39 30
EcoRI G'AATT_C
Cuts at: 3399 3399
Size: 6397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4414 4414
Size: 6397
FseI GG_CCGG'CC
Cuts at: 182 182
Size: 6397
FspI TGC'GCA
Cuts at: 4176 4176
Size: 6397
HaeII r_GCGC'y
Cuts at: 189 1362 1796 1871 2011 2087 2107 2578 2637
Size: 1173 434 75 140 76 20 471 59
Cuts at: 2637 3829 4077 5101 6080 189
Size: 1192 248 1024 979 506
Fragments arranged by size:
1192 1173 1024 979 506 471 434 248
140 76 75 59 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 979 1011 4237 4267 4269 4299 979
Size: 32 3226 30 2 30 3077
Fragments arranged by size:
3226 3077 32 30 30 2
HincII GTy'rAC
Cuts at: 2145 3434 2145
Size: 1289 5108
KasI G'GCGC_C
Cuts at: 1867 2083 3825 4073 1867
Size: 216 1742 248 4191
Fragments arranged by size:
4191 1742 248 216
KpnI G_GTAC'C
Cuts at: 3415 3415
Size: 6397
MluI A'CGCG_T
Cuts at: 5830 5830
Size: 6397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 117 2396 5068 5252 117
Size: 2279 2672 184 1262
Fragments arranged by size:
2672 2279 1262 184
MscI TGG'CCA
Cuts at: 130 469 604 2014 2071 2382 4156 130
Size: 339 135 1410 57 311 1774 2371
Fragments arranged by size:
2371 1774 1410 339 311 135 57
MslI CAynn'nnrTG
Cuts at: 598 1498 2230 2402 2677 3085 4384 4511 598
Size: 900 732 172 275 408 1299 127 2484
Fragments arranged by size:
2484 1299 900 732 408 275 172 127
MspA1I CmG'CkG
Cuts at: 281 425 787 888 1458 1509 1636 1974 1983
Size: 144 362 101 570 51 127 338 9
Cuts at: 1983 2366 2702 2768 3014 3381 3506 3820 4180
Size: 383 336 66 246 367 125 314 360
Cuts at: 4180 5195 5440 281
Size: 1015 245 1238
Fragments arranged by size:
1238 1015 570 383 367 362 360 338
336 314 246 245 144 127 125 101
66 51 9
NaeI GCC'GGC
Cuts at: 180 699 933 1062 1947 3249 4576 180
Size: 519 234 129 885 1302 1327 2001
Fragments arranged by size:
2001 1327 1302 885 519 234 129
NarI GG'CG_CC
Cuts at: 1868 2084 3826 4074 1868
Size: 216 1742 248 4191
Fragments arranged by size:
4191 1742 248 216
NcoI C'CATG_G
Cuts at: 125 125
Size: 6397
NgoMIV G'CCGG_C
Cuts at: 178 697 931 1060 1945 3247 4574 178
Size: 519 234 129 885 1302 1327 2001
Fragments arranged by size:
2001 1327 1302 885 519 234 129
NruI TCG'CGA
Cuts at: 4758 4758
Size: 6397
NspI r_CATG'y
Cuts at: 1497 1929 2278 3448 3948 4857 1497
Size: 432 349 1170 500 909 3037
Fragments arranged by size:
3037 1170 909 500 432 349
PasI CC'CwG_GG
Cuts at: 806 951 806
Size: 145 6252
PciI A'CATG_T
Cuts at: 4853 4853
Size: 6397
PflMI CCAn_nnn'nTGG
Cuts at: 2661 2919 3270 2661
Size: 258 351 5788
Fragments arranged by size:
5788 351 258
PfoI T'CCnGG_A
Cuts at: 677 1238 2288 2353 2618 677
Size: 561 1050 65 265 4456
Fragments arranged by size:
4456 1050 561 265 65
PmeI GTTT'AAAC
Cuts at: 3394 3394
Size: 6397
PmlI CAC'GTG
Cuts at: 1500 1721 1901 2916 3123 1500
Size: 221 180 1015 207 4774
Fragments arranged by size:
4774 1015 221 207 180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2208 5536 5568 2176
Size: 32 3328 32 3005
Fragments arranged by size:
3328 3005 32 32
PpuMI rG'GwC_Cy
Cuts at: 381 810 2883 3213 3326 381
Size: 429 2073 330 113 3452
Fragments arranged by size:
3452 2073 429 330 113
PshAI GACnn'nnGTC
Cuts at: 1320 1320
Size: 6397
PspOMI G'GGCC_C
Cuts at: 627 780 1612 3370 627
Size: 153 832 1758 3654
Fragments arranged by size:
3654 1758 832 153
PspXI vC'TCGA_Gb
Cuts at: 2934 2934
Size: 6397
PstI C_TGCA'G
Cuts at: 845 893 940 1763 2111 3442 4127 845
Size: 48 47 823 348 1331 685 3115
Fragments arranged by size:
3115 1331 823 685 348 48 47
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6397
PvuII CAG'CTG
Cuts at: 425 787 888 1983 2366 2768 3820 4180 425
Size: 362 101 1095 383 402 1052 360 2642
Fragments arranged by size:
2642 1095 1052 402 383 362 360 101
RsrII CG'GwC_CG
Cuts at: 4590 4590
Size: 6397
SacI G_AGCT'C
Cuts at: 2414 3409 2414
Size: 995 5402
SacII CC_GC'GG
Cuts at: 1637 3015 1637
Size: 1378 5019
SalI G'TCGA_C
Cuts at: 3432 3432
Size: 6397
SanDI GG'GwC_CC
Cuts at: 810 2883 3326 810
Size: 2073 443 3881
Fragments arranged by size:
3881 2073 443
SapI GCTCTTCn'nnn_
Cuts at: 2620 4418 4628 2620
Size: 1798 210 4389
Fragments arranged by size:
4389 1798 210
SbfI CC_TGCA'GG
Cuts at: 1763 3442 1763
Size: 1679 4718
ScaI AGT'ACT
Cuts at: 5914 5914
Size: 6397
SexAI A'CCwGG_T
Cuts at: 1153 1823 1874 1153
Size: 670 51 5676
Fragments arranged by size:
5676 670 51
SfcI C'TryA_G
Cuts at: 33 841 889 936 1759 2107 3438 4123 5118
Size: 808 48 47 823 348 1331 685 995
Cuts at: 5118 5309 33
Size: 191 1121
Fragments arranged by size:
1331 1121 995 823 808 685 348 191
48 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 1336 2388 1336
Size: 1052 5345
SfoI GGC'GCC
Cuts at: 1869 2085 3827 4075 1869
Size: 216 1742 248 4191
Fragments arranged by size:
4191 1742 248 216
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6397
SgrAI Cr'CCGG_yG
Cuts at: 897 2080 897
Size: 1183 5214
SmaI CCC'GGG
Cuts at: 741 1364 1611 3417 741
Size: 623 247 1806 3721
Fragments arranged by size:
3721 1806 623 247
SmlI C'TyrA_G
Cuts at: 2934 3550 4465 4959 5221 2934
Size: 616 915 494 262 4110
Fragments arranged by size:
4110 915 616 494 262
SnaBI TAC'GTA
Cuts at: 3387 3387
Size: 6397
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6397
SphI G_CATG'C
Cuts at: 1497 2278 3448 3948 1497
Size: 781 1170 500 3946
Fragments arranged by size:
3946 1170 781 500
SrfI GCCC'GGGC
Cuts at: 1364 1364
Size: 6397
StuI AGG'CCT
Cuts at: 313 2066 2863 3112 3295 313
Size: 1753 797 249 183 3415
Fragments arranged by size:
3415 1753 797 249 183
StyI C'CwwG_G
Cuts at: 125 416 1535 3107 3320 3529 3883 125
Size: 291 1119 1572 213 209 354 2639
Fragments arranged by size:
2639 1572 1119 354 291 213 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1276 1977 2191 3277 3978 4111 4570 4781 1276
Size: 701 214 1086 701 133 459 211 2892
Fragments arranged by size:
2892 1086 701 701 459 214 211 133
TatI w'GTAC_w
Cuts at: 2927 5912 2927
Size: 2985 3412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 386 418 719 751 1094 1126 1885 1917 5914
Size: 32 301 32 343 32 759 32 3997
Cuts at: 5914 5946 386
Size: 32 837
Fragments arranged by size:
3997 837 759 343 301 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2464 4192 2464
Size: 1728 4669
XbaI T'CTAG_A
Cuts at: 3426 3426
Size: 6397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1914 2064 2559 3890 1914
Size: 150 495 1331 4421
Fragments arranged by size:
4421 1331 495 150
XhoI C'TCGA_G
Cuts at: 2934 2934
Size: 6397
XmaI C'CCGG_G
Cuts at: 739 1362 1609 3415 739
Size: 623 247 1806 3721
Fragments arranged by size:
3721 1806 623 247
ZraI GAC'GTC
Cuts at: 2775 2775
Size: 6397
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AscI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsmI BsmBI BstEII BstZ17I ClaI CspCI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PsiI PsrI SspI SwaI XmnI