Restriction map for
pF1KSDA2013
(Circular) (Six-base) MAPSORT of: pF1KSDA2013.seq Check: 3860 from: 1 to: 5140
pF1KSDA2013 5140 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 1823 278
Size: 1545 3595
AccI GT'mk_AC
Cuts at: 728 1300 2176 728
Size: 572 876 3692
Fragments arranged by size:
3692 876 572
Acc65I G'GTAC_C
Cuts at: 372 2154 372
Size: 1782 3358
AclI AA'CG_TT
Cuts at: 4980 4980
Size: 5140
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 1134 1311 1986 2963 3395 4144 4412 551
Size: 583 177 675 977 432 749 268 1279
Fragments arranged by size:
1279 977 749 675 583 432 268 177
AflIII A'CryG_T
Cuts at: 1647 1649 2490 3596 4573 1647
Size: 2 841 1106 977 2214
Fragments arranged by size:
2214 1106 977 841 2
AgeI A'CCGG_T
Cuts at: 3528 3528
Size: 5140
AhdI GACnn_n'nnGTC
Cuts at: 724 724
Size: 5140
AlwNI CAG_nnn'CTG
Cuts at: 610 1395 1824 2443 4012 610
Size: 785 429 619 1569 1738
Fragments arranged by size:
1738 1569 785 619 429
ApaI G_GGCC'C
Cuts at: 183 638 794 183
Size: 455 156 4529
Fragments arranged by size:
4529 455 156
ApaLI G'TGCA_C
Cuts at: 901 991 3910 901
Size: 90 2919 2131
Fragments arranged by size:
2919 2131 90
ApoI r'AATT_y
Cuts at: 2142 4493 4749 2142
Size: 2351 256 2533
Fragments arranged by size:
2533 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5140
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5140
AvaI C'yCGr_G
Cuts at: 457 631 1888 2039 2158 457
Size: 174 1257 151 119 3439
Fragments arranged by size:
3439 1257 174 151 119
BamHI G'GATC_C
Cuts at: 2163 2163
Size: 5140
BanI G'GyrC_C
Cuts at: 298 372 480 1462 1734 2154 2568 2816 2851
Size: 74 108 982 272 420 414 248 35
Cuts at: 2851 298
Size: 2587
Fragments arranged by size:
2587 982 420 414 272 248 108 74
35
BanII G_rGCy'C
Cuts at: 183 473 638 794 1049 1091 1143 2152 3182
Size: 290 165 156 255 42 52 1009 1030
Cuts at: 3182 183
Size: 2141
Fragments arranged by size:
2141 1030 1009 290 255 165 156 52
42
BbeI G_GCGC'C
Cuts at: 302 484 1738 2572 2820 302
Size: 182 1254 834 248 2622
Fragments arranged by size:
2622 1254 834 248 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 329 295
Size: 34 5106
BciVI GTATCCnnnnn_n'
Cuts at: 3036 3799 5140 3036
Size: 763 1341 3036
Fragments arranged by size:
3036 1341 763
BclI T'GATC_A
Cuts at: 2658 2658
Size: 5140
BfrBI ATG'CAT
Cuts at: 1570 1570
Size: 5140
BglI GCCn_nnn'nGGC
Cuts at: 203 403 814 1371 1618 1696 1855 203
Size: 200 411 557 247 78 159 3488
Fragments arranged by size:
3488 557 411 247 200 159 78
BglII A'GATC_T
Cuts at: 1682 2653 4692 1682
Size: 971 2039 2130
Fragments arranged by size:
2130 2039 971
BlpI GC'TnA_GC
Cuts at: 517 1142 1258 1492 2250 517
Size: 625 116 234 758 3407
Fragments arranged by size:
3407 758 625 234 116
Bme1580I G_kGCm'C
Cuts at: 183 497 638 794 905 995 1337 1467 2763
Size: 314 141 156 111 90 342 130 1296
Cuts at: 2763 2856 3914 183
Size: 93 1058 1409
Fragments arranged by size:
1409 1296 1058 342 314 156 141 130
111 93 90
BmgBI CAC'GTC
Cuts at: 429 726 996 1551 429
Size: 297 270 555 4018
Fragments arranged by size:
4018 555 297 270
BmrI ACTGGGnnnn_n'
Cuts at: 902 2350 2431 2766 4912 902
Size: 1448 81 335 2146 1130
Fragments arranged by size:
2146 1448 1130 335 81
BmtI G_CTAG'C
Cuts at: 2086 2086
Size: 5140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1746 3505 1746
Size: 1759 3381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 407 557 853 1105 1411 1513 2006 343
Size: 64 150 296 252 306 102 493 3477
Fragments arranged by size:
3477 493 306 296 252 150 102 64
Bpu10I CC'TnA_GC
Cuts at: 1270 1270
Size: 5140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 1033 1828 2314 3193 3687 3985 130
Size: 903 795 486 879 494 298 1285
Fragments arranged by size:
1285 903 879 795 494 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 839 1280 4844 839
Size: 441 3564 1135
Fragments arranged by size:
3564 1135 441
BsaAI yAC'GTr
Cuts at: 1650 2130 2491 1650
Size: 480 361 4299
Fragments arranged by size:
4299 480 361
BsaBI GATnn'nnATC
Cuts at: 6 2652 4691 6
Size: 2646 2039 455
Fragments arranged by size:
2646 2039 455
BsaHI Gr'CG_yC
Cuts at: 299 481 1735 2569 2817 3521 5017 299
Size: 182 1254 834 248 704 1496 422
Fragments arranged by size:
1496 1254 834 704 422 248 182
BsaWI w'CCGG_w
Cuts at: 2327 2848 3528 3802 3949 4360 2327
Size: 521 680 274 147 411 3107
Fragments arranged by size:
3107 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 530 560 1219 1249 530
Size: 30 659 30 4421
Fragments arranged by size:
4421 659 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 850 1123 181
Size: 669 273 4198
Fragments arranged by size:
4198 669 273
BseYI C'CCAG_C
Cuts at: 176 378 594 804 1877 2563 3321 3900 4396
Size: 202 216 210 1073 686 758 579 496
Cuts at: 4396 176
Size: 920
Fragments arranged by size:
1073 920 758 686 579 496 216 210
202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 523 1540 1822 250
Size: 273 1017 282 3568
Fragments arranged by size:
3568 1017 282 273
BsiEI CG_ry'CG
Cuts at: 104 672 2680 2726 3936 104
Size: 568 2008 46 1210 1308
Fragments arranged by size:
2008 1308 1210 568 46
BsiHKAI G_wGCw'C
Cuts at: 905 995 1025 1049 1091 1493 1871 2152 2930
Size: 90 30 24 42 402 378 281 778
Cuts at: 2930 3120 3914 905
Size: 190 794 2131
Fragments arranged by size:
2131 794 778 402 378 281 190 90
42 30 24
BsmBI CGTCTCn'nnnn_
Cuts at: 651 967 1002 651
Size: 316 35 4789
Fragments arranged by size:
4789 316 35
Bsp1286I G_dGCh'C
Cuts at: 183 473 497 638 794 905 995 1025 1049
Size: 290 24 141 156 111 90 30 24
Cuts at: 1049 1091 1143 1337 1467 1493 1871 2152 2763
Size: 42 52 194 130 26 378 281 611
Cuts at: 2763 2856 2930 3120 3182 3914 183
Size: 93 74 190 62 732 1409
Fragments arranged by size:
1409 732 611 378 290 281 194 190
156 141 130 111 93 90 74 62
52 42 30 26 24 24
BspEI T'CCGG_A
Cuts at: 4360 4360
Size: 5140
BspHI T'CATG_A
Cuts at: 4316 4316
Size: 5140
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 740 1823 1832 2188 2402 2704 3085 278
Size: 462 1083 9 356 214 302 381 2333
Fragments arranged by size:
2333 1083 462 381 356 302 214 9
BsrBI CCG'CTC
Cuts at: 50 465 3430 3484 4970 5135 50
Size: 415 2965 54 1486 165 55
Fragments arranged by size:
2965 1486 415 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1570 3050 1570
Size: 1480 3660
BsrFI r'CCGG_y
Cuts at: 393 625 799 872 3136 3317 3528 393
Size: 232 174 73 2264 181 211 2005
Fragments arranged by size:
2264 2005 232 211 181 174 73
BssHII G'CGCG_C
Cuts at: 242 521 242
Size: 279 4861
BssSI C'ACGA_G
Cuts at: 3409 3769 3409
Size: 360 4780
BstAPI GCAn_nnn'nTGC
Cuts at: 273 1395 1824 1836 2515 273
Size: 1122 429 12 679 2898
Fragments arranged by size:
2898 1122 679 429 12
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5140
BstXI CCAn_nnnn'nTGG
Cuts at: 831 897 930 1435 831
Size: 66 33 505 4536
Fragments arranged by size:
4536 505 66 33
BstYI r'GATC_y
Cuts at: 1 1391 1682 2163 2630 2653 2988 3234 4248
Size: 1390 291 481 467 23 335 246 1014
Cuts at: 4248 4334 4346 4692 1
Size: 86 12 346 449
Fragments arranged by size:
1390 1014 481 467 449 346 335 291
246 86 23 12
BtgI C'CryG_G
Cuts at: 285 476 617 659 758 1655 1665 1739 285
Size: 191 141 42 99 897 10 74 3686
Fragments arranged by size:
3686 897 191 141 99 74 42 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3088 3269 3432 3088
Size: 181 163 4796
Fragments arranged by size:
4796 181 163
BtsI GCAGTG_nn'
Cuts at: 794 1252 1320 1783 2381 794
Size: 458 68 463 598 3553
Fragments arranged by size:
3553 598 463 458 68
DraI TTT'AAA
Cuts at: 2137 2137
Size: 5140
DraIII CAC_nnn'GTG
Cuts at: 909 909
Size: 5140
DrdI GACnn_nn'nnGTC
Cuts at: 2844 3704 2844
Size: 860 4280
EaeI y'GGCC_r
Cuts at: 329 404 620 669 770 917 1363 1610 1668
Size: 75 216 49 101 147 446 247 58
Cuts at: 1668 2723 2897 3288 3315 4662 329
Size: 1055 174 391 27 1347 807
Fragments arranged by size:
1347 1055 807 446 391 247 216 174
147 101 75 58 49 27
EagI C'GGCC_G
Cuts at: 669 2723 669
Size: 2054 3086
EarI CTCTTCn'nnn_
Cuts at: 1153 1909 1942 1951 1957 2005 3161 3371 1153
Size: 756 33 9 6 48 1156 210 2922
Fragments arranged by size:
2922 1156 756 210 48 33 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1123 1636 3346 3658 3804 4950 1123
Size: 513 1710 312 146 1146 1313
Fragments arranged by size:
1710 1313 1146 513 312 146
EcoICRI GAG'CTC
Cuts at: 1047 1089 2150 1047
Size: 42 1061 4037
Fragments arranged by size:
4037 1061 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 407 551 557 853 1105 1134 1311 1411
Size: 64 144 6 296 252 29 177 100
Cuts at: 1411 1513 1986 2006 2963 3395 4144 4412 343
Size: 102 473 20 957 432 749 268 1071
Fragments arranged by size:
1071 957 749 473 432 296 268 252
177 144 102 100 64 29 20 6
EcoNI CCTnn'n_nnAGG
Cuts at: 835 835
Size: 5140
EcoO109I rG'GnC_Cy
Cuts at: 382 599 607 790 1821 1884 2277 382
Size: 217 8 183 1031 63 393 3245
Fragments arranged by size:
3245 1031 393 217 183 63 8
EcoRI G'AATT_C
Cuts at: 2142 2142
Size: 5140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2016 3157 2016
Size: 1141 3999
FspI TGC'GCA
Cuts at: 2919 2919
Size: 5140
HaeII r_GCGC'y
Cuts at: 302 484 1057 1738 2572 2820 3844 4823 302
Size: 182 573 681 834 248 1024 979 619
Fragments arranged by size:
1024 979 834 681 619 573 248 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 710 742 2980 3010 3012 3042 710
Size: 32 2238 30 2 30 2808
Fragments arranged by size:
2808 2238 32 30 30 2
HincII GTy'rAC
Cuts at: 1301 2177 1301
Size: 876 4264
KasI G'GCGC_C
Cuts at: 298 480 1734 2568 2816 298
Size: 182 1254 834 248 2622
Fragments arranged by size:
2622 1254 834 248 182
KpnI G_GTAC'C
Cuts at: 376 2158 376
Size: 1782 3358
MluI A'CGCG_T
Cuts at: 4573 4573
Size: 5140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 822 3811 3995 822
Size: 2989 184 1967
Fragments arranged by size:
2989 1967 184
MscI TGG'CCA
Cuts at: 406 1670 2899 406
Size: 1264 1229 2647
Fragments arranged by size:
2647 1264 1229
MslI CAynn'nnrTG
Cuts at: 3127 3254 3127
Size: 127 5013
MspA1I CmG'CkG
Cuts at: 141 176 287 378 507 516 594 661 966
Size: 35 111 91 129 9 78 67 305
Cuts at: 966 1105 1331 2249 2563 2923 3938 4183 141
Size: 139 226 918 314 360 1015 245 1098
Fragments arranged by size:
1098 1015 918 360 314 305 245 226
139 129 111 91 78 67 35 9
NaeI GCC'GGC
Cuts at: 395 627 801 3319 395
Size: 232 174 2518 2216
Fragments arranged by size:
2518 2216 232 174
NarI GG'CG_CC
Cuts at: 299 481 1735 2569 2817 299
Size: 182 1254 834 248 2622
Fragments arranged by size:
2622 1254 834 248 182
NgoMIV G'CCGG_C
Cuts at: 393 625 799 3317 393
Size: 232 174 2518 2216
Fragments arranged by size:
2518 2216 232 174
NheI G'CTAG_C
Cuts at: 2082 2082
Size: 5140
NruI TCG'CGA
Cuts at: 3501 3501
Size: 5140
NsiI A_TGCA'T
Cuts at: 1572 1572
Size: 5140
NspI r_CATG'y
Cuts at: 1101 1233 2191 2691 3600 1101
Size: 132 958 500 909 2641
Fragments arranged by size:
2641 958 909 500 132
PciI A'CATG_T
Cuts at: 3596 3596
Size: 5140
PflMI CCAn_nnn'nTGG
Cuts at: 610 610
Size: 5140
PfoI T'CCnGG_A
Cuts at: 384 384
Size: 5140
PmeI GTTT'AAAC
Cuts at: 2137 2137
Size: 5140
PmlI CAC'GTG
Cuts at: 1650 1650
Size: 5140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4279 4311 4279
Size: 32 5108
PpuMI rG'GwC_Cy
Cuts at: 382 599 607 1821 1884 382
Size: 217 8 1214 63 3638
Fragments arranged by size:
3638 1214 217 63 8
PshAI GACnn'nnGTC
Cuts at: 165 165
Size: 5140
PspOMI G'GGCC_C
Cuts at: 179 634 790 179
Size: 455 156 4529
Fragments arranged by size:
4529 455 156
PstI C_TGCA'G
Cuts at: 137 593 737 782 1061 1400 1481 1511 1820
Size: 456 144 45 279 339 81 30 309
Cuts at: 1820 2185 2870 137
Size: 365 685 2407
Fragments arranged by size:
2407 685 456 365 339 309 279 144
81 45 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5140
PvuII CAG'CTG
Cuts at: 176 507 594 966 2563 2923 176
Size: 331 87 372 1597 360 2393
Fragments arranged by size:
2393 1597 372 360 331 87
RsrII CG'GwC_CG
Cuts at: 3333 3333
Size: 5140
SacI G_AGCT'C
Cuts at: 1049 1091 2152 1049
Size: 42 1061 4037
Fragments arranged by size:
4037 1061 42
SacII CC_GC'GG
Cuts at: 288 662 288
Size: 374 4766
SalI G'TCGA_C
Cuts at: 1299 2175 1299
Size: 876 4264
SanDI GG'GwC_CC
Cuts at: 599 599
Size: 5140
SapI GCTCTTCn'nnn_
Cuts at: 1153 3161 3371 1153
Size: 2008 210 2922
Fragments arranged by size:
2922 2008 210
SbfI CC_TGCA'GG
Cuts at: 1481 1820 2185 1481
Size: 339 365 4436
Fragments arranged by size:
4436 365 339
ScaI AGT'ACT
Cuts at: 1975 4657 1975
Size: 2682 2458
SexAI A'CCwGG_T
Cuts at: 905 905
Size: 5140
SfcI C'TryA_G
Cuts at: 33 133 589 733 778 1057 1396 1477 1507
Size: 100 456 144 45 279 339 81 30
Cuts at: 1507 1816 2181 2866 3861 4052 33
Size: 309 365 685 995 191 1121
Fragments arranged by size:
1121 995 685 456 365 339 309 279
191 144 100 81 45 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 403 1855 403
Size: 1452 3688
SfoI GGC'GCC
Cuts at: 300 482 1736 2570 2818 300
Size: 182 1254 834 248 2622
Fragments arranged by size:
2622 1254 834 248 182
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5140
SmaI CCC'GGG
Cuts at: 459 633 1890 2160 459
Size: 174 1257 270 3439
Fragments arranged by size:
3439 1257 270 174
SmlI C'TyrA_G
Cuts at: 145 1048 1843 2293 3208 3702 3964 145
Size: 903 795 450 915 494 262 1321
Fragments arranged by size:
1321 915 903 795 494 450 262
SnaBI TAC'GTA
Cuts at: 2130 2130
Size: 5140
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5140
SphI G_CATG'C
Cuts at: 2191 2691 2191
Size: 500 4640
StuI AGG'CCT
Cuts at: 817 2045 2106 817
Size: 1228 61 3851
Fragments arranged by size:
3851 1228 61
StyI C'CwwG_G
Cuts at: 812 1040 2046 2272 2626 812
Size: 228 1006 226 354 3326
Fragments arranged by size:
3326 1006 354 228 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 438 975 1201 1450 1746 2109 2721 2854 3313
Size: 537 226 249 296 363 612 133 459
Cuts at: 3313 3524 438
Size: 211 2054
Fragments arranged by size:
2054 612 537 459 363 296 249 226
211 133
TatI w'GTAC_w
Cuts at: 1973 4655 1973
Size: 2682 2458
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 530 562 4657 4689 530
Size: 32 4095 32 981
Fragments arranged by size:
4095 981 32 32
Tth111I GACn'n_nGTC
Cuts at: 2935 2935
Size: 5140
XbaI T'CTAG_A
Cuts at: 2169 2169
Size: 5140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 765 963 1195 2633 414
Size: 351 198 232 1438 2921
Fragments arranged by size:
2921 1438 351 232 198
XmaI C'CCGG_G
Cuts at: 457 631 1888 2158 457
Size: 174 1257 270 3439
Fragments arranged by size:
3439 1257 270 174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NruI NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NotI PacI PasI PsiI PspXI
PsrI SgrAI SrfI SspI SwaI XhoI XmnI ZraI