Restriction map for
pF1KSDA2005
(Circular) (Six-base) MAPSORT of: pF1KSDA2005.seq Check: 5864 from: 1 to: 7891
pF1KSDA2005 7891 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 2558 3351 3503 3970 4927 2558
Size: 793 152 467 957 5522
Fragments arranged by size:
5522 957 793 467 152
Acc65I G'GTAC_C
Cuts at: 2365 4905 2365
Size: 2540 5351
AclI AA'CG_TT
Cuts at: 333 7731 333
Size: 7398 493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1286 1676 1763 2033 2228 2394 2764 3742 4485
Size: 390 87 270 195 166 370 978 743
Cuts at: 4485 4733 5714 6146 6895 7163 1286
Size: 248 981 432 749 268 2014
Fragments arranged by size:
2014 981 978 749 743 432 390 370
270 268 248 195 166 87
AflII C'TTAA_G
Cuts at: 205 205
Size: 7891
AflIII A'CryG_T
Cuts at: 1562 3695 5241 6347 7324 1562
Size: 2133 1546 1106 977 2129
Fragments arranged by size:
2133 2129 1546 1106 977
AgeI A'CCGG_T
Cuts at: 6279 6279
Size: 7891
AleI CACnn'nnGTG
Cuts at: 754 3076 754
Size: 2322 5569
AlwNI CAG_nnn'CTG
Cuts at: 5194 6763 5194
Size: 1569 6322
ApaLI G'TGCA_C
Cuts at: 6661 6661
Size: 7891
ApoI r'AATT_y
Cuts at: 230 462 772 813 1103 1230 1750 2149 3180
Size: 232 310 41 290 127 520 399 1031
Cuts at: 3180 3214 3471 3900 4269 4635 4893 7244 7500
Size: 34 257 429 369 366 258 2351 256
Cuts at: 7500 230
Size: 621
Fragments arranged by size:
2351 1031 621 520 429 399 369 366
310 290 258 257 256 232 127 41
34
AseI AT'TA_AT
Cuts at: 20 212 3298 20
Size: 192 3086 4613
Fragments arranged by size:
4613 3086 192
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7891
AvaI C'yCGr_G
Cuts at: 4909 4909
Size: 7891
AvrII C'CTAG_G
Cuts at: 4830 4830
Size: 7891
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3060 3093 3060
Size: 33 7858
BamHI G'GATC_C
Cuts at: 4056 4914 4056
Size: 858 7033
BanI G'GyrC_C
Cuts at: 829 2365 2734 4905 5319 5567 5602 829
Size: 1536 369 2171 414 248 35 3118
Fragments arranged by size:
3118 2171 1536 414 369 248 35
BanII G_rGCy'C
Cuts at: 4903 5933 4903
Size: 1030 6861
BbeI G_GCGC'C
Cuts at: 5323 5571 5323
Size: 248 7643
BbsI GAAGACnn'nnnn_
Cuts at: 206 780 1553 3014 206
Size: 574 773 1461 5083
Fragments arranged by size:
5083 1461 773 574
BciVI GTATCCnnnnn_n'
Cuts at: 2214 5787 6550 7891 2214
Size: 3573 763 1341 2214
Fragments arranged by size:
3573 2214 1341 763
BclI T'GATC_A
Cuts at: 630 926 1478 2468 2795 5409 630
Size: 296 552 990 327 2614 3112
Fragments arranged by size:
3112 2614 990 552 327 296
BfrBI ATG'CAT
Cuts at: 2382 3370 2382
Size: 988 6903
BglII A'GATC_T
Cuts at: 1899 3912 5404 7443 1899
Size: 2013 1492 2039 2347
Fragments arranged by size:
2347 2039 2013 1492
BlpI GC'TnA_GC
Cuts at: 5001 5001
Size: 7891
Bme1580I G_kGCm'C
Cuts at: 3444 5514 5607 6665 3444
Size: 2070 93 1058 4670
Fragments arranged by size:
4670 2070 1058 93
BmgBI CAC'GTC
Cuts at: 3445 3445
Size: 7891
BmrI ACTGGGnnnn_n'
Cuts at: 3447 5101 5182 5517 7663 3447
Size: 1654 81 335 2146 3675
Fragments arranged by size:
3675 2146 1654 335 81
BmtI G_CTAG'C
Cuts at: 1169 1169
Size: 7891
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1819 6256 1819
Size: 4437 3454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 4078 2176
Size: 1902 5989
Bpu10I CC'TnA_GC
Cuts at: 3387 3387
Size: 7891
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3357 3588 5065 5944 6438 6736 3357
Size: 231 1477 879 494 298 4512
Fragments arranged by size:
4512 1477 879 494 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 7595 7595
Size: 7891
BsaAI yAC'GTr
Cuts at: 4881 5242 4881
Size: 361 7530
BsaBI GATnn'nnATC
Cuts at: 6 488 798 5403 7442 6
Size: 482 310 4605 2039 455
Fragments arranged by size:
4605 2039 482 455 310
BsaHI Gr'CG_yC
Cuts at: 5320 5568 6272 7768 5320
Size: 248 704 1496 5443
Fragments arranged by size:
5443 1496 704 248
BsaWI w'CCGG_w
Cuts at: 5078 5599 6279 6553 6700 7111 5078
Size: 521 680 274 147 411 5858
Fragments arranged by size:
5858 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 857 887 973 1003 3825 3855 857
Size: 30 86 30 2822 30 4893
Fragments arranged by size:
4893 2822 86 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 4114 4114
Size: 7891
BseYI C'CCAG_C
Cuts at: 1603 2297 5314 6072 6651 7147 1603
Size: 694 3017 758 579 496 2347
Fragments arranged by size:
3017 2347 758 694 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 3362 4545 2072
Size: 1290 1183 5418
Fragments arranged by size:
5418 1290 1183
BsiEI CG_ry'CG
Cuts at: 104 5431 5477 6687 104
Size: 5327 46 1210 1308
Fragments arranged by size:
5327 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 693 4903 5681 5871 6665 693
Size: 4210 778 190 794 1919
Fragments arranged by size:
4210 1919 794 778 190
BsmI GAATG_Cn'
Cuts at: 3370 3370
Size: 7891
BsmBI CGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 7891
Bsp1286I G_dGCh'C
Cuts at: 693 3444 4903 5514 5607 5681 5871 5933 6665
Size: 2751 1459 611 93 74 190 62 732
Cuts at: 6665 693
Size: 1919
Fragments arranged by size:
2751 1919 1459 732 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 7111 7111
Size: 7891
BspHI T'CATG_A
Cuts at: 378 2792 2798 7067 378
Size: 2414 6 4269 1202
Fragments arranged by size:
4269 2414 1202 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4517 4939 5153 5455 5836 4517
Size: 422 214 302 381 6572
Fragments arranged by size:
6572 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6181 6235 7721 7886 50
Size: 6131 54 1486 165 55
Fragments arranged by size:
6131 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 786 1295 5801 786
Size: 509 4506 2876
Fragments arranged by size:
4506 2876 509
BsrFI r'CCGG_y
Cuts at: 5887 6068 6279 5887
Size: 181 211 7499
Fragments arranged by size:
7499 211 181
BsrGI T'GTAC_A
Cuts at: 2116 2116
Size: 7891
BssSI C'ACGA_G
Cuts at: 6160 6520 6160
Size: 360 7531
BstAPI GCAn_nnn'nTGC
Cuts at: 234 2455 5266 234
Size: 2221 2811 2859
Fragments arranged by size:
2859 2811 2221
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7891
BstEII G'GTnAC_C
Cuts at: 2478 3665 2478
Size: 1187 6704
BstXI CCAn_nnnn'nTGG
Cuts at: 756 1673 756
Size: 917 6974
BstYI r'GATC_y
Cuts at: 1 1797 1899 2041 2647 3912 4056 4495 4914
Size: 1796 102 142 606 1265 144 439 419
Cuts at: 4914 5381 5404 5739 5985 6999 7085 7097 7443
Size: 467 23 335 246 1014 86 12 346
Cuts at: 7443 1
Size: 449
Fragments arranged by size:
1796 1265 1014 606 467 449 439 419
346 335 246 144 142 102 86 23
12
BstZ17I GTA'TAC
Cuts at: 3971 3971
Size: 7891
BtgI C'CryG_G
Cuts at: 307 749 861 2037 4193 307
Size: 442 112 1176 2156 4005
Fragments arranged by size:
4005 2156 1176 442 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 634 5839 6020 6183 634
Size: 5205 181 163 2342
Fragments arranged by size:
5205 2342 181 163
BtsI GCAGTG_nn'
Cuts at: 2292 5132 2292
Size: 2840 5051
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 974 1009 974
Size: 35 7856
DraI TTT'AAA
Cuts at: 1235 1430 1961 4888 1235
Size: 195 531 2927 4238
Fragments arranged by size:
4238 2927 531 195
DraIII CAC_nnn'GTG
Cuts at: 2950 2950
Size: 7891
DrdI GACnn_nn'nnGTC
Cuts at: 1033 5595 6455 1033
Size: 4562 860 2469
Fragments arranged by size:
4562 2469 860
EaeI y'GGCC_r
Cuts at: 1184 4280 4429 5474 5648 6039 6066 7413 1184
Size: 3096 149 1045 174 391 27 1347 1662
Fragments arranged by size:
3096 1662 1347 1045 391 174 149 27
EagI C'GGCC_G
Cuts at: 5474 5474
Size: 7891
EarI CTCTTCn'nnn_
Cuts at: 1265 1782 3170 3201 3242 5912 6122 1265
Size: 517 1388 31 41 2670 210 3034
Fragments arranged by size:
3034 2670 1388 517 210 41 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6097 6409 6555 7701 6097
Size: 312 146 1146 6287
Fragments arranged by size:
6287 1146 312 146
EcoICRI GAG'CTC
Cuts at: 4901 4901
Size: 7891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1286 1676 1763 2033 2176 2228 2394 2764 3742
Size: 390 87 270 143 52 166 370 978
Cuts at: 3742 4078 4485 4733 5714 6146 6895 7163 1286
Size: 336 407 248 981 432 749 268 2014
Fragments arranged by size:
2014 981 978 749 432 407 390 370
336 270 268 248 166 143 87 52
EcoNI CCTnn'n_nnAGG
Cuts at: 683 683
Size: 7891
EcoO109I rG'GnC_Cy
Cuts at: 285 4399 4849 5028 285
Size: 4114 450 179 3148
Fragments arranged by size:
4114 3148 450 179
EcoRI G'AATT_C
Cuts at: 813 4893 813
Size: 4080 3811
EcoRV GAT'ATC
Cuts at: 510 1179 510
Size: 669 7222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 918 2424 3754 4838 5908 918
Size: 1506 1330 1084 1070 2901
Fragments arranged by size:
2901 1506 1330 1084 1070
FspI TGC'GCA
Cuts at: 5670 5670
Size: 7891
HaeII r_GCGC'y
Cuts at: 5323 5571 6595 7574 5323
Size: 248 1024 979 5640
Fragments arranged by size:
5640 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1456 1488 1566 1598 3649 3681 3825 3857 5731
Size: 32 78 32 2051 32 144 32 1874
Cuts at: 5731 5761 5763 5793 1456
Size: 30 2 30 3554
Fragments arranged by size:
3554 2051 1874 144 78 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2868 4928 2868
Size: 2060 5831
HindIII A'AGCT_T
Cuts at: 1496 2275 3000 3305 1496
Size: 779 725 305 6082
Fragments arranged by size:
6082 779 725 305
KasI G'GCGC_C
Cuts at: 5319 5567 5319
Size: 248 7643
KpnI G_GTAC'C
Cuts at: 2369 4909 2369
Size: 2540 5351
MfeI C'AATT_G
Cuts at: 604 604
Size: 7891
MluI A'CGCG_T
Cuts at: 7324 7324
Size: 7891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1436 3509 6562 6746 1436
Size: 2073 3053 184 2581
Fragments arranged by size:
3053 2581 2073 184
MscI TGG'CCA
Cuts at: 1186 4282 4431 5650 1186
Size: 3096 149 1219 3427
Fragments arranged by size:
3427 3096 1219 149
MslI CAynn'nnrTG
Cuts at: 754 766 917 1096 1671 2515 2797 3076 5878
Size: 12 151 179 575 844 282 279 2802
Cuts at: 5878 6005 754
Size: 127 2640
Fragments arranged by size:
2802 2640 844 575 282 279 179 151
127 12
MspA1I CmG'CkG
Cuts at: 1607 5000 5314 5674 6689 6934 1607
Size: 3393 314 360 1015 245 2564
Fragments arranged by size:
3393 2564 1015 360 314 245
NaeI GCC'GGC
Cuts at: 6070 6070
Size: 7891
NarI GG'CG_CC
Cuts at: 5320 5568 5320
Size: 248 7643
NcoI C'CATG_G
Cuts at: 307 861 4193 307
Size: 554 3332 4005
Fragments arranged by size:
4005 3332 554
NdeI CA'TA_TG
Cuts at: 3806 4860 3806
Size: 1054 6837
NgoMIV G'CCGG_C
Cuts at: 6068 6068
Size: 7891
NheI G'CTAG_C
Cuts at: 1165 1165
Size: 7891
NruI TCG'CGA
Cuts at: 6252 6252
Size: 7891
NsiI A_TGCA'T
Cuts at: 2384 3372 2384
Size: 988 6903
NspI r_CATG'y
Cuts at: 181 1566 2386 3699 4524 4942 5442 6351 181
Size: 1385 820 1313 825 418 500 909 1721
Fragments arranged by size:
1721 1385 1313 909 825 820 500 418
PasI CC'CwG_GG
Cuts at: 2987 2987
Size: 7891
PciI A'CATG_T
Cuts at: 1562 3695 6347 1562
Size: 2133 2652 3106
Fragments arranged by size:
3106 2652 2133
PflMI CCAn_nnn'nTGG
Cuts at: 2359 2359
Size: 7891
PfoI T'CCnGG_A
Cuts at: 384 2153 2650 4094 384
Size: 1769 497 1444 4181
Fragments arranged by size:
4181 1769 1444 497
PmeI GTTT'AAAC
Cuts at: 4888 4888
Size: 7891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7030 7062 7030
Size: 32 7859
PpuMI rG'GwC_Cy
Cuts at: 285 4399 285
Size: 4114 3777
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3012 3044 3012
Size: 32 7859
PstI C_TGCA'G
Cuts at: 269 1010 4232 4936 5621 269
Size: 741 3222 704 685 2539
Fragments arranged by size:
3222 2539 741 704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7891
PvuII CAG'CTG
Cuts at: 1607 5314 5674 1607
Size: 3707 360 3824
Fragments arranged by size:
3824 3707 360
RsrII CG'GwC_CG
Cuts at: 6084 6084
Size: 7891
SacI G_AGCT'C
Cuts at: 4903 4903
Size: 7891
SalI G'TCGA_C
Cuts at: 4926 4926
Size: 7891
SapI GCTCTTCn'nnn_
Cuts at: 5912 6122 5912
Size: 210 7681
SbfI CC_TGCA'GG
Cuts at: 269 4936 269
Size: 4667 3224
ScaI AGT'ACT
Cuts at: 4612 7408 4612
Size: 2796 5095
SfcI C'TryA_G
Cuts at: 33 265 1006 2484 3546 4228 4730 4932 5617
Size: 232 741 1478 1062 682 502 202 685
Cuts at: 5617 6612 6803 33
Size: 995 191 1121
Fragments arranged by size:
1478 1121 1062 995 741 685 682 502
232 202 191
SfoI GGC'GCC
Cuts at: 5321 5569 5321
Size: 248 7643
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7891
SmaI CCC'GGG
Cuts at: 4911 4911
Size: 7891
SmlI C'TyrA_G
Cuts at: 205 3336 3603 5044 5959 6453 6715 205
Size: 3131 267 1441 915 494 262 1381
Fragments arranged by size:
3131 1441 1381 915 494 267 262
SnaBI TAC'GTA
Cuts at: 4881 4881
Size: 7891
SpeI A'CTAG_T
Cuts at: 67 2977 67
Size: 2910 4981
SphI G_CATG'C
Cuts at: 4942 5442 4942
Size: 500 7391
SspI AAT'ATT
Cuts at: 480 2321 2957 480
Size: 1841 636 5414
Fragments arranged by size:
5414 1841 636
StyI C'CwwG_G
Cuts at: 307 861 985 2714 4193 4830 5023 5377 307
Size: 554 124 1729 1479 637 193 354 2821
Fragments arranged by size:
2821 1729 1479 637 554 354 193 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2000 3709 5472 5605 6064 6275 2000
Size: 1709 1763 133 459 211 3616
Fragments arranged by size:
3616 1763 1709 459 211 133
TatI w'GTAC_w
Cuts at: 2116 2980 3107 4511 4610 7406 2116
Size: 864 127 1404 99 2796 2601
Fragments arranged by size:
2796 2601 1404 864 127 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1936 1968 2081 2113 7408 7440 1936
Size: 32 113 32 5295 32 2387
Fragments arranged by size:
5295 2387 113 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2010 4382 5686 2010
Size: 2372 1304 4215
Fragments arranged by size:
4215 2372 1304
XbaI T'CTAG_A
Cuts at: 1252 1659 4920 1252
Size: 407 3261 4223
Fragments arranged by size:
4223 3261 407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3804 5384 3804
Size: 1580 6311
XmaI C'CCGG_G
Cuts at: 4909 4909
Size: 7891
XmnI GAAnn'nnTTC
Cuts at: 527 1001 2407 3024 3184 527
Size: 474 1406 617 160 5234
Fragments arranged by size:
5234 1406 617 474 160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
BbvCI BcgI BglI BsiWI BssHII Bsu36I ClaI FseI
FspAI HpaI NotI PacI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI