Restriction map for
pF1KSDA1990
(Circular) (Six-base) MAPSORT of: pF1KSDA1990.seq Check: 6958 from: 1 to: 5776
pF1KSDA1990 5776 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 1976 1976
Size: 5776
AccI GT'mk_AC
Cuts at: 2192 2812 2192
Size: 620 5156
Acc65I G'GTAC_C
Cuts at: 2790 2790
Size: 5776
AclI AA'CG_TT
Cuts at: 1358 1380 5616 1358
Size: 22 4236 1518
Fragments arranged by size:
4236 1518 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1477 1682 3599 4031 4780 5048 565
Size: 912 205 1917 432 749 268 1293
Fragments arranged by size:
1917 1293 912 749 432 268 205
AflII C'TTAA_G
Cuts at: 1930 1930
Size: 5776
AflIII A'CryG_T
Cuts at: 1700 3126 4232 5209 1700
Size: 1426 1106 977 2267
Fragments arranged by size:
2267 1426 1106 977
AgeI A'CCGG_T
Cuts at: 137 1483 4164 137
Size: 1346 2681 1749
Fragments arranged by size:
2681 1749 1346
AleI CACnn'nnGTG
Cuts at: 292 292
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1429 1429
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 377 3079 4648 377
Size: 2702 1569 1505
Fragments arranged by size:
2702 1569 1505
ApaLI G'TGCA_C
Cuts at: 637 2575 4546 637
Size: 1938 1971 1867
Fragments arranged by size:
1971 1938 1867
ApoI r'AATT_y
Cuts at: 995 2778 5129 5385 995
Size: 1783 2351 256 1386
Fragments arranged by size:
2351 1783 1386 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5776
AvaI C'yCGr_G
Cuts at: 667 2012 2342 2794 667
Size: 1345 330 452 3649
Fragments arranged by size:
3649 1345 452 330
BamHI G'GATC_C
Cuts at: 476 2114 2799 476
Size: 1638 685 3453
Fragments arranged by size:
3453 1638 685
BanI G'GyrC_C
Cuts at: 2790 3204 3452 3487 2790
Size: 414 248 35 5079
Fragments arranged by size:
5079 414 248 35
BanII G_rGCy'C
Cuts at: 1392 2489 2788 3818 1392
Size: 1097 299 1030 3350
Fragments arranged by size:
3350 1097 1030 299
BbeI G_GCGC'C
Cuts at: 3208 3456 3208
Size: 248 5528
BbsI GAAGACnn'nnnn_
Cuts at: 165 677 1112 165
Size: 512 435 4829
Fragments arranged by size:
4829 512 435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1225 1259 1225
Size: 34 5742
BciVI GTATCCnnnnn_n'
Cuts at: 589 907 2656 3672 4435 5776 589
Size: 318 1749 1016 763 1341 589
Fragments arranged by size:
1749 1341 1016 763 589 318
BclI T'GATC_A
Cuts at: 3294 3294
Size: 5776
BfrBI ATG'CAT
Cuts at: 660 660
Size: 5776
BglII A'GATC_T
Cuts at: 740 3289 5328 740
Size: 2549 2039 1188
Fragments arranged by size:
2549 2039 1188
BlpI GC'TnA_GC
Cuts at: 2886 2886
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 289 641 2579 3399 3492 4550 289
Size: 352 1938 820 93 1058 1515
Fragments arranged by size:
1938 1515 1058 820 352 93
BmgBI CAC'GTC
Cuts at: 212 696 212
Size: 484 5292
BmrI ACTGGGnnnn_n'
Cuts at: 1119 1745 2986 3067 3402 5548 1119
Size: 626 1241 81 335 2146 1347
Fragments arranged by size:
2146 1347 1241 626 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 612 4141 612
Size: 3529 2247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 1105 1192 1378 1987 2042 2569 815
Size: 290 87 186 609 55 527 4022
Fragments arranged by size:
4022 609 527 290 186 87 55
Bpu10I CC'TnA_GC
Cuts at: 391 391
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2950 3829 4323 4621 2950
Size: 879 494 298 4105
Fragments arranged by size:
4105 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 796 940 1195 5480 796
Size: 144 255 4285 1092
Fragments arranged by size:
4285 1092 255 144
BsaAI yAC'GTr
Cuts at: 2766 3127 2766
Size: 361 5415
BsaBI GATnn'nnATC
Cuts at: 6 2653 3288 5327 6
Size: 2647 635 2039 455
Fragments arranged by size:
2647 2039 635 455
BsaHI Gr'CG_yC
Cuts at: 1973 2384 3205 3453 4157 5653 1973
Size: 411 821 248 704 1496 2096
Fragments arranged by size:
2096 1496 821 704 411 248
BsaWI w'CCGG_w
Cuts at: 137 749 1483 2231 2495 2963 3484 4164 4438
Size: 612 734 748 264 468 521 680 274
Cuts at: 4438 4585 4996 137
Size: 147 411 917
Fragments arranged by size:
917 748 734 680 612 521 468 411
274 264 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1756 1786 1756
Size: 30 5746
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 619 898 1161 1319 1496 619
Size: 279 263 158 177 4899
Fragments arranged by size:
4899 279 263 177 158
BseYI C'CCAG_C
Cuts at: 867 1469 2239 3199 3957 4536 5032 867
Size: 602 770 960 758 579 496 1611
Fragments arranged by size:
1611 960 770 758 602 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 1270
Size: 5776
BsiEI CG_ry'CG
Cuts at: 104 1518 3316 3362 4572 104
Size: 1414 1798 46 1210 1308
Fragments arranged by size:
1798 1414 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 397 641 2579 2788 3566 3756 4550 397
Size: 244 1938 209 778 190 794 1623
Fragments arranged by size:
1938 1623 794 778 244 209 190
BsmI GAATG_Cn'
Cuts at: 829 2241 829
Size: 1412 4364
Bsp1286I G_dGCh'C
Cuts at: 289 397 641 1392 2489 2579 2788 3399 3492
Size: 108 244 751 1097 90 209 611 93
Cuts at: 3492 3566 3756 3818 4550 289
Size: 74 190 62 732 1515
Fragments arranged by size:
1515 1097 751 732 611 244 209 190
108 93 90 74 62
BspEI T'CCGG_A
Cuts at: 2231 4996 2231
Size: 2765 3011
BspHI T'CATG_A
Cuts at: 1178 1886 4952 1178
Size: 708 3066 2002
Fragments arranged by size:
3066 2002 708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1899 1967 2824 3038 3340 3721 1899
Size: 68 857 214 302 381 3954
Fragments arranged by size:
3954 857 381 302 214 68
BsrBI CCG'CTC
Cuts at: 50 4066 4120 5606 5771 50
Size: 4016 54 1486 165 55
Fragments arranged by size:
4016 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3686 3686
Size: 5776
BsrFI r'CCGG_y
Cuts at: 137 888 1483 1517 3772 3953 4164 137
Size: 751 595 34 2255 181 211 1749
Fragments arranged by size:
2255 1749 751 595 211 181 34
BssSI C'ACGA_G
Cuts at: 4045 4405 4045
Size: 360 5416
BstAPI GCAn_nnn'nTGC
Cuts at: 718 3151 718
Size: 2433 3343
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 609 1545 1782 2322 609
Size: 936 237 540 4063
Fragments arranged by size:
4063 936 540 237
BstYI r'GATC_y
Cuts at: 1 476 740 2114 2799 3266 3289 3624 3870
Size: 475 264 1374 685 467 23 335 246
Cuts at: 3870 4884 4970 4982 5328 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1374 1014 685 475 467 449 346 335
264 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2193 2193
Size: 5776
BtgI C'CryG_G
Cuts at: 233 233
Size: 5776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2640 3724 3905 4068 2640
Size: 1084 181 163 4348
Fragments arranged by size:
4348 1084 181 163
BtsI GCAGTG_nn'
Cuts at: 395 683 1253 2535 3017 395
Size: 288 570 1282 482 3154
Fragments arranged by size:
3154 1282 570 482 288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 959 994 1812 1847 959
Size: 35 818 35 4888
Fragments arranged by size:
4888 818 35 35
DraI TTT'AAA
Cuts at: 2773 2773
Size: 5776
DraIII CAC_nnn'GTG
Cuts at: 1806 1806
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 1909 3480 4340 1909
Size: 1571 860 3345
Fragments arranged by size:
3345 1571 860
EaeI y'GGCC_r
Cuts at: 611 849 2214 3359 3533 3924 3951 5298 611
Size: 238 1365 1145 174 391 27 1347 1089
Fragments arranged by size:
1365 1347 1145 1089 391 238 174 27
EagI C'GGCC_G
Cuts at: 3359 3359
Size: 5776
EarI CTCTTCn'nnn_
Cuts at: 1774 2258 2597 2713 3797 4007 1774
Size: 484 339 116 1084 210 3543
Fragments arranged by size:
3543 1084 484 339 210 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3982 4294 4440 5586 3982
Size: 312 146 1146 4172
Fragments arranged by size:
4172 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2786 2786
Size: 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 815 1105 1192 1378 1477 1682 1987 2042
Size: 250 290 87 186 99 205 305 55
Cuts at: 2042 2569 3599 4031 4780 5048 565
Size: 527 1030 432 749 268 1293
Fragments arranged by size:
1293 1030 749 527 432 305 290 268
250 205 186 99 87 55
EcoO109I rG'GnC_Cy
Cuts at: 1596 2913 1596
Size: 1317 4459
EcoRI G'AATT_C
Cuts at: 2778 2778
Size: 5776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3793 3793
Size: 5776
FspI TGC'GCA
Cuts at: 3555 3555
Size: 5776
HaeII r_GCGC'y
Cuts at: 2556 2724 3208 3456 4480 5459 2556
Size: 168 484 248 1024 979 2873
Fragments arranged by size:
2873 1024 979 484 248 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 263 295 739 771 1080 1112 1437 1469 1690
Size: 32 444 32 309 32 325 32 221
Cuts at: 1690 1714 1722 1746 1995 2014 2027 2046 2144
Size: 24 8 24 249 19 13 19 98
Cuts at: 2144 2176 3616 3646 3648 3678 263
Size: 32 1440 30 2 30 2361
Fragments arranged by size:
2361 1440 444 325 309 249 221 98
32 32 32 32 32 30 30 24
24 19 19 13 8 2
HincII GTy'rAC
Cuts at: 840 2813 840
Size: 1973 3803
KasI G'GCGC_C
Cuts at: 3204 3452 3204
Size: 248 5528
KpnI G_GTAC'C
Cuts at: 2794 2794
Size: 5776
MluI A'CGCG_T
Cuts at: 5209 5209
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 135 902 1921 4447 4631 135
Size: 767 1019 2526 184 1280
Fragments arranged by size:
2526 1280 1019 767 184
MscI TGG'CCA
Cuts at: 851 2216 3535 851
Size: 1365 1319 3092
Fragments arranged by size:
3092 1365 1319
MslI CAynn'nnrTG
Cuts at: 292 1699 3763 3890 292
Size: 1407 2064 127 2178
Fragments arranged by size:
2178 2064 1407 127
MspA1I CmG'CkG
Cuts at: 217 691 1083 1469 2885 3199 3559 4574 4819
Size: 474 392 386 1416 314 360 1015 245
Cuts at: 4819 217
Size: 1174
Fragments arranged by size:
1416 1174 1015 474 392 386 360 314
245
NaeI GCC'GGC
Cuts at: 3955 3955
Size: 5776
NarI GG'CG_CC
Cuts at: 3205 3453 3205
Size: 248 5528
NcoI C'CATG_G
Cuts at: 233 233
Size: 5776
NgoMIV G'CCGG_C
Cuts at: 3953 3953
Size: 5776
NruI TCG'CGA
Cuts at: 2381 4137 2381
Size: 1756 4020
NsiI A_TGCA'T
Cuts at: 662 662
Size: 5776
NspI r_CATG'y
Cuts at: 664 910 1704 1821 1944 2191 2827 3327 4236
Size: 246 794 117 123 247 636 500 909
Cuts at: 4236 664
Size: 2204
Fragments arranged by size:
2204 909 794 636 500 247 246 123
117
PciI A'CATG_T
Cuts at: 1700 4232 1700
Size: 2532 3244
PfoI T'CCnGG_A
Cuts at: 1964 2438 1964
Size: 474 5302
PmeI GTTT'AAAC
Cuts at: 2773 2773
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 178 210 4915 4947 178
Size: 32 4705 32 1007
Fragments arranged by size:
4705 1007 32 32
PpuMI rG'GwC_Cy
Cuts at: 1596 1596
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 2337 2337
Size: 5776
PspXI vC'TCGA_Gb
Cuts at: 2012 2012
Size: 5776
PstI C_TGCA'G
Cuts at: 959 1248 1855 2396 2821 3506 959
Size: 289 607 541 425 685 3229
Fragments arranged by size:
3229 685 607 541 425 289
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5776
PvuII CAG'CTG
Cuts at: 217 1083 3199 3559 217
Size: 866 2116 360 2434
Fragments arranged by size:
2434 2116 866 360
RsrII CG'GwC_CG
Cuts at: 3969 3969
Size: 5776
SacI G_AGCT'C
Cuts at: 2788 2788
Size: 5776
SalI G'TCGA_C
Cuts at: 2811 2811
Size: 5776
SapI GCTCTTCn'nnn_
Cuts at: 2258 2597 3797 4007 2258
Size: 339 1200 210 4027
Fragments arranged by size:
4027 1200 339 210
SbfI CC_TGCA'GG
Cuts at: 2821 2821
Size: 5776
ScaI AGT'ACT
Cuts at: 1576 5293 1576
Size: 3717 2059
SexAI A'CCwGG_T
Cuts at: 370 644 370
Size: 274 5502
SfcI C'TryA_G
Cuts at: 33 955 1099 1244 1759 1851 2392 2535 2541
Size: 922 144 145 515 92 541 143 6
Cuts at: 2541 2817 3502 4497 4688 33
Size: 276 685 995 191 1121
Fragments arranged by size:
1121 995 922 685 541 515 276 191
145 144 143 92 6
SfoI GGC'GCC
Cuts at: 3206 3454 3206
Size: 248 5528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5776
SmaI CCC'GGG
Cuts at: 2796 2796
Size: 5776
SmlI C'TyrA_G
Cuts at: 1930 2012 2929 3844 4338 4600 1930
Size: 82 917 915 494 262 3106
Fragments arranged by size:
3106 917 915 494 262 82
SnaBI TAC'GTA
Cuts at: 2766 2766
Size: 5776
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5776
SphI G_CATG'C
Cuts at: 1821 2827 3327 1821
Size: 1006 500 4270
Fragments arranged by size:
4270 1006 500
StyI C'CwwG_G
Cuts at: 233 941 2473 2678 2908 3262 233
Size: 708 1532 205 230 354 2747
Fragments arranged by size:
2747 1532 708 354 230 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 545 820 2728 3357 3490 3949 4160 262
Size: 283 275 1908 629 133 459 211 1878
Fragments arranged by size:
1908 1878 629 459 283 275 211 133
TatI w'GTAC_w
Cuts at: 1574 1985 5291 1574
Size: 411 3306 2059
Fragments arranged by size:
3306 2059 411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1672 1704 5293 5325 1672
Size: 32 3589 32 2123
Fragments arranged by size:
3589 2123 32 32
Tth111I GACn'n_nGTC
Cuts at: 3571 3571
Size: 5776
XbaI T'CTAG_A
Cuts at: 2805 2805
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2436 3269 2436
Size: 833 4943
XhoI C'TCGA_G
Cuts at: 2012 2012
Size: 5776
XmaI C'CCGG_G
Cuts at: 2794 2794
Size: 5776
ZraI GAC'GTC
Cuts at: 1974 1974
Size: 5776
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PciI PfoI PmeI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AloI ApaI AscI AvrII BaeI
BbvCI BglI BmtI BsiWI BsmBI BsrGI BssHII BstEII
Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI PacI PasI PflMI PmlI
PsiI PspOMI PsrI SacII SanDI SfiI SgrAI SrfI
SspI StuI SwaI XmnI