Restriction map for
pF1KSDA1970
(Circular) (Six-base) MAPSORT of: pF1KSDA1970.seq Check: 643 from: 1 to: 4708
pF1KSDA1970 4708 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 636 693 905 953 636
Size: 57 212 48 4391
Fragments arranged by size:
4391 212 57 48
AccI GT'mk_AC
Cuts at: 1316 1385 1744 1316
Size: 69 359 4280
Fragments arranged by size:
4280 359 69
Acc65I G'GTAC_C
Cuts at: 1722 1722
Size: 4708
AclI AA'CG_TT
Cuts at: 4548 4548
Size: 4708
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 595 1552 2531 2963 3712 3980 541
Size: 54 957 979 432 749 268 1269
Fragments arranged by size:
1269 979 957 749 432 268 54
AfeI AGC'GCT
Cuts at: 515 515
Size: 4708
AflIII A'CryG_T
Cuts at: 2058 3164 4141 2058
Size: 1106 977 2625
Fragments arranged by size:
2625 1106 977
AgeI A'CCGG_T
Cuts at: 3096 3096
Size: 4708
AhdI GACnn_n'nnGTC
Cuts at: 1374 1374
Size: 4708
AleI CACnn'nnGTG
Cuts at: 278 278
Size: 4708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1034 1120 1034
Size: 86 4622
AlwNI CAG_nnn'CTG
Cuts at: 564 660 726 915 1209 2011 3580 564
Size: 96 66 189 294 802 1569 1692
Fragments arranged by size:
1692 1569 802 294 189 96 66
ApaI G_GGCC'C
Cuts at: 467 467
Size: 4708
ApaLI G'TGCA_C
Cuts at: 3478 3478
Size: 4708
ApoI r'AATT_y
Cuts at: 1183 1710 4061 4317 1183
Size: 527 2351 256 1574
Fragments arranged by size:
2351 1574 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4708
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4708
AvaI C'yCGr_G
Cuts at: 1726 1726
Size: 4708
BamHI G'GATC_C
Cuts at: 1328 1673 1731 1328
Size: 345 58 4305
Fragments arranged by size:
4305 345 58
BanI G'GyrC_C
Cuts at: 708 1232 1558 1722 2136 2384 2419 708
Size: 524 326 164 414 248 35 2997
Fragments arranged by size:
2997 524 414 326 248 164 35
BanII G_rGCy'C
Cuts at: 442 467 572 1720 2750 442
Size: 25 105 1148 1030 2400
Fragments arranged by size:
2400 1148 1030 105 25
BbeI G_GCGC'C
Cuts at: 1236 2140 2388 1236
Size: 904 248 3556
Fragments arranged by size:
3556 904 248
BbsI GAAGACnn'nnnn_
Cuts at: 529 529
Size: 4708
BciVI GTATCCnnnnn_n'
Cuts at: 2604 3367 4708 2604
Size: 763 1341 2604
Fragments arranged by size:
2604 1341 763
BclI T'GATC_A
Cuts at: 1115 2226 1115
Size: 1111 3597
BglI GCCn_nnn'nGGC
Cuts at: 263 263
Size: 4708
BglII A'GATC_T
Cuts at: 1489 2221 4260 1489
Size: 732 2039 1937
Fragments arranged by size:
2039 1937 732
BlpI GC'TnA_GC
Cuts at: 1818 1818
Size: 4708
Bme1580I G_kGCm'C
Cuts at: 217 467 2331 2424 3482 217
Size: 250 1864 93 1058 1443
Fragments arranged by size:
1864 1443 1058 250 93
BmrI ACTGGGnnnn_n'
Cuts at: 769 1306 1375 1918 1999 2334 4480 769
Size: 537 69 543 81 335 2146 997
Fragments arranged by size:
2146 997 543 537 335 81 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1523 3073 1523
Size: 1550 3158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 586 718 1027 1186 1189 430
Size: 156 132 309 159 3 3949
Fragments arranged by size:
3949 309 159 156 132 3
Bpu10I CC'TnA_GC
Cuts at: 1071 1071
Size: 4708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 2761 3255 3553 1882
Size: 879 494 298 3037
Fragments arranged by size:
3037 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 764 4412 764
Size: 3648 1060
BsaAI yAC'GTr
Cuts at: 855 1320 1698 2059 855
Size: 465 378 361 3504
Fragments arranged by size:
3504 465 378 361
BsaBI GATnn'nnATC
Cuts at: 6 2220 4259 6
Size: 2214 2039 455
Fragments arranged by size:
2214 2039 455
BsaHI Gr'CG_yC
Cuts at: 604 1233 1433 2137 2385 3089 4585 604
Size: 629 200 704 248 704 1496 727
Fragments arranged by size:
1496 727 704 704 629 248 200
BsaWI w'CCGG_w
Cuts at: 526 1895 2416 3096 3370 3517 3928 526
Size: 1369 521 680 274 147 411 1306
Fragments arranged by size:
1369 1306 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 761 791 1184 1214 761
Size: 30 393 30 4255
Fragments arranged by size:
4255 393 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 156 892 946 1282 156
Size: 736 54 336 3582
Fragments arranged by size:
3582 736 336 54
BseYI C'CCAG_C
Cuts at: 253 460 557 1473 2131 2889 3468 3964 253
Size: 207 97 916 658 758 579 496 997
Fragments arranged by size:
997 916 758 658 579 496 207 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 645 1180 645
Size: 535 4173
BsiEI CG_ry'CG
Cuts at: 104 2248 2294 3504 104
Size: 2144 46 1210 1308
Fragments arranged by size:
2144 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 572 1013 1425 1720 2498 2688 3482 572
Size: 441 412 295 778 190 794 1798
Fragments arranged by size:
1798 794 778 441 412 295 190
BsmBI CGTCTCn'nnnn_
Cuts at: 885 885
Size: 4708
Bsp1286I G_dGCh'C
Cuts at: 217 442 467 572 1013 1425 1720 2331 2424
Size: 225 25 105 441 412 295 611 93
Cuts at: 2424 2498 2688 2750 3482 217
Size: 74 190 62 732 1443
Fragments arranged by size:
1443 732 611 441 412 295 225 190
105 93 74 62 25
BspEI T'CCGG_A
Cuts at: 3928 3928
Size: 4708
BspHI T'CATG_A
Cuts at: 782 3884 782
Size: 3102 1606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 636 693 905 953 1245 1756 1970 2272 2653
Size: 57 212 48 292 511 214 302 381
Cuts at: 2653 636
Size: 2691
Fragments arranged by size:
2691 511 381 302 292 214 212 57
48
BsrBI CCG'CTC
Cuts at: 50 2998 3052 4538 4703 50
Size: 2948 54 1486 165 55
Fragments arranged by size:
2948 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1224 2618 1224
Size: 1394 3314
BsrFI r'CCGG_y
Cuts at: 2704 2885 3096 2704
Size: 181 211 4316
Fragments arranged by size:
4316 211 181
BsrGI T'GTAC_A
Cuts at: 299 299
Size: 4708
BssSI C'ACGA_G
Cuts at: 2977 3337 2977
Size: 360 4348
BstAPI GCAn_nnn'nTGC
Cuts at: 708 2083 708
Size: 1375 3333
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4708
BstEII G'GTnAC_C
Cuts at: 1140 1140
Size: 4708
BstYI r'GATC_y
Cuts at: 1 1328 1489 1673 1731 2198 2221 2556 2802
Size: 1327 161 184 58 467 23 335 246
Cuts at: 2802 3816 3902 3914 4260 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1327 1014 467 449 346 335 246 184
161 86 58 23 12
BstZ17I GTA'TAC
Cuts at: 1386 1386
Size: 4708
Bsu36I CC'TnA_GG
Cuts at: 139 343 139
Size: 204 4504
BtgI C'CryG_G
Cuts at: 125 125
Size: 4708
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2656 2837 3000 2656
Size: 181 163 4364
Fragments arranged by size:
4364 181 163
BtsI GCAGTG_nn'
Cuts at: 290 884 1949 290
Size: 594 1065 3049
Fragments arranged by size:
3049 1065 594
DraI TTT'AAA
Cuts at: 1705 1705
Size: 4708
DrdI GACnn_nn'nnGTC
Cuts at: 2412 3272 2412
Size: 860 3848
EaeI y'GGCC_r
Cuts at: 180 662 755 2291 2465 2856 2883 4230 180
Size: 482 93 1536 174 391 27 1347 658
Fragments arranged by size:
1536 1347 658 482 391 174 93 27
EagI C'GGCC_G
Cuts at: 2291 2291
Size: 4708
EarI CTCTTCn'nnn_
Cuts at: 781 2729 2939 781
Size: 1948 210 2550
Fragments arranged by size:
2550 1948 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 682 2914 3226 3372 4518 682
Size: 2232 312 146 1146 872
Fragments arranged by size:
2232 1146 872 312 146
EcoICRI GAG'CTC
Cuts at: 570 1718 570
Size: 1148 3560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 541 586 595 718 1027 1186 1189 1552
Size: 111 45 9 123 309 159 3 363
Cuts at: 1552 2531 2963 3712 3980 430
Size: 979 432 749 268 1158
Fragments arranged by size:
1158 979 749 432 363 309 268 159
123 111 45 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 577 700 907 1135 1264 1412 577
Size: 123 207 228 129 148 3873
Fragments arranged by size:
3873 228 207 148 129 123
EcoO109I rG'GnC_Cy
Cuts at: 464 669 723 912 1100 1257 1618 1845 464
Size: 205 54 189 188 157 361 227 3327
Fragments arranged by size:
3327 361 227 205 189 188 157 54
EcoRI G'AATT_C
Cuts at: 1183 1710 1183
Size: 527 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 2725 603
Size: 2122 2586
FspI TGC'GCA
Cuts at: 2487 2487
Size: 4708
HaeII r_GCGC'y
Cuts at: 136 517 1236 2140 2388 3412 4391 136
Size: 381 719 904 248 1024 979 453
Fragments arranged by size:
1024 979 904 719 453 381 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 761 793 1184 1216 1332 1360 1364 1392 1423
Size: 32 391 32 116 28 4 28 31
Cuts at: 1423 1455 2548 2578 2580 2610 761
Size: 32 1093 30 2 30 2859
Fragments arranged by size:
2859 1093 391 116 32 32 32 31
30 30 28 28 4 2
HincII GTy'rAC
Cuts at: 1745 1745
Size: 4708
KasI G'GCGC_C
Cuts at: 1232 2136 2384 1232
Size: 904 248 3556
Fragments arranged by size:
3556 904 248
KpnI G_GTAC'C
Cuts at: 1726 1726
Size: 4708
MluI A'CGCG_T
Cuts at: 4141 4141
Size: 4708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 467 1028 3379 3563 467
Size: 561 2351 184 1612
Fragments arranged by size:
2351 1612 561 184
MscI TGG'CCA
Cuts at: 664 757 2467 664
Size: 93 1710 2905
Fragments arranged by size:
2905 1710 93
MslI CAynn'nnrTG
Cuts at: 278 2695 2822 278
Size: 2417 127 2164
Fragments arranged by size:
2417 2164 127
MspA1I CmG'CkG
Cuts at: 388 483 561 1206 1239 1287 1428 1817 2131
Size: 95 78 645 33 48 141 389 314
Cuts at: 2131 2491 3506 3751 388
Size: 360 1015 245 1345
Fragments arranged by size:
1345 1015 645 389 360 314 245 141
95 78 48 33
NaeI GCC'GGC
Cuts at: 2887 2887
Size: 4708
NarI GG'CG_CC
Cuts at: 1233 2137 2385 1233
Size: 904 248 3556
Fragments arranged by size:
3556 904 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4708
NgoMIV G'CCGG_C
Cuts at: 2885 2885
Size: 4708
NruI TCG'CGA
Cuts at: 3069 3069
Size: 4708
NspI r_CATG'y
Cuts at: 408 1759 2259 3168 408
Size: 1351 500 909 1948
Fragments arranged by size:
1948 1351 909 500
PasI CC'CwG_GG
Cuts at: 915 915
Size: 4708
PciI A'CATG_T
Cuts at: 3164 3164
Size: 4708
PflMI CCAn_nnn'nTGG
Cuts at: 564 660 726 840 915 1209 1242 1683 564
Size: 96 66 114 75 294 33 441 3589
Fragments arranged by size:
3589 441 294 114 96 75 66 33
PfoI T'CCnGG_A
Cuts at: 719 1004 719
Size: 285 4423
PmeI GTTT'AAAC
Cuts at: 1705 1705
Size: 4708
PmlI CAC'GTG
Cuts at: 855 855
Size: 4708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3847 3879 3847
Size: 32 4676
PpuMI rG'GwC_Cy
Cuts at: 669 723 1100 1257 1618 669
Size: 54 377 157 361 3759
Fragments arranged by size:
3759 377 361 157 54
PshAI GACnn'nnGTC
Cuts at: 1138 1138
Size: 4708
PspOMI G'GGCC_C
Cuts at: 463 463
Size: 4708
PstI C_TGCA'G
Cuts at: 155 1256 1367 1413 1753 2438 155
Size: 1101 111 46 340 685 2425
Fragments arranged by size:
2425 1101 685 340 111 46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4708
PvuII CAG'CTG
Cuts at: 1239 1287 1428 2131 2491 1239
Size: 48 141 703 360 3456
Fragments arranged by size:
3456 703 360 141 48
RsrII CG'GwC_CG
Cuts at: 2901 2901
Size: 4708
SacI G_AGCT'C
Cuts at: 572 1720 572
Size: 1148 3560
SalI G'TCGA_C
Cuts at: 1743 1743
Size: 4708
SapI GCTCTTCn'nnn_
Cuts at: 781 2729 2939 781
Size: 1948 210 2550
Fragments arranged by size:
2550 1948 210
SbfI CC_TGCA'GG
Cuts at: 1367 1753 1367
Size: 386 4322
ScaI AGT'ACT
Cuts at: 4225 4225
Size: 4708
SexAI A'CCwGG_T
Cuts at: 725 725
Size: 4708
SfcI C'TryA_G
Cuts at: 33 151 1252 1363 1409 1625 1749 2434 3429
Size: 118 1101 111 46 216 124 685 995
Cuts at: 3429 3620 33
Size: 191 1121
Fragments arranged by size:
1121 1101 995 685 216 191 124 118
111 46
SfoI GGC'GCC
Cuts at: 1234 2138 2386 1234
Size: 904 248 3556
Fragments arranged by size:
3556 904 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4708
SmaI CCC'GGG
Cuts at: 1728 1728
Size: 4708
SmlI C'TyrA_G
Cuts at: 1861 2776 3270 3532 1861
Size: 915 494 262 3037
Fragments arranged by size:
3037 915 494 262
SnaBI TAC'GTA
Cuts at: 1698 1698
Size: 4708
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4708
SphI G_CATG'C
Cuts at: 1759 2259 1759
Size: 500 4208
SspI AAT'ATT
Cuts at: 396 396
Size: 4708
StuI AGG'CCT
Cuts at: 165 586 1273 165
Size: 421 687 3600
Fragments arranged by size:
3600 687 421
StyI C'CwwG_G
Cuts at: 125 665 1646 1840 2194 125
Size: 540 981 194 354 2639
Fragments arranged by size:
2639 981 540 354 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 2289 2422 2881 3092 268
Size: 2021 133 459 211 1884
Fragments arranged by size:
2021 1884 459 211 133
TatI w'GTAC_w
Cuts at: 299 1565 4223 299
Size: 1266 2658 784
Fragments arranged by size:
2658 1266 784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4225 4257 4225
Size: 32 4676
Tth111I GACn'n_nGTC
Cuts at: 727 775 1346 2503 727
Size: 48 571 1157 2932
Fragments arranged by size:
2932 1157 571 48
XbaI T'CTAG_A
Cuts at: 1482 1737 1482
Size: 255 4453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 810 1470 2201 810
Size: 660 731 3317
Fragments arranged by size:
3317 731 660
XmaI C'CCGG_G
Cuts at: 1726 1726
Size: 4708
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BssHII BstXI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SwaI XhoI XmnI
ZraI