Restriction map for
pF1KSDA1960
(Circular) (Six-base) MAPSORT of: pF1KSDA1960.seq Check: 1635 from: 1 to: 5695
pF1KSDA1960 5695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 595 595
Size: 5695
AccI GT'mk_AC
Cuts at: 2731 2731
Size: 5695
Acc65I G'GTAC_C
Cuts at: 2709 2709
Size: 5695
AclI AA'CG_TT
Cuts at: 5535 5535
Size: 5695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1002 1031 2095 2431 2548 3518 3950 4699
Size: 458 29 1064 336 117 970 432 749
Cuts at: 4699 4967 544
Size: 268 1272
Fragments arranged by size:
1272 1064 970 749 458 432 336 268
117 29
AfeI AGC'GCT
Cuts at: 1044 1044
Size: 5695
AflIII A'CryG_T
Cuts at: 3045 4151 5128 3045
Size: 1106 977 3612
Fragments arranged by size:
3612 1106 977
AgeI A'CCGG_T
Cuts at: 2469 4083 2469
Size: 1614 4081
AhdI GACnn_n'nnGTC
Cuts at: 993 993
Size: 5695
AleI CACnn'nnGTG
Cuts at: 2157 2157
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 640 1497 640
Size: 857 4838
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 580 612 973 1005 580
Size: 32 361 32 5270
Fragments arranged by size:
5270 361 32 32
AlwNI CAG_nnn'CTG
Cuts at: 748 1465 1770 1965 2220 2998 4567 748
Size: 717 305 195 255 778 1569 1876
Fragments arranged by size:
1876 1569 778 717 305 255 195
ApaI G_GGCC'C
Cuts at: 340 890 1358 340
Size: 550 468 4677
Fragments arranged by size:
4677 550 468
ApaLI G'TGCA_C
Cuts at: 4465 4465
Size: 5695
ApoI r'AATT_y
Cuts at: 2697 5048 5304 2697
Size: 2351 256 3088
Fragments arranged by size:
3088 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5695
AvaI C'yCGr_G
Cuts at: 253 333 426 435 534 699 728 889 955
Size: 80 93 9 99 165 29 161 66
Cuts at: 955 1750 1976 2318 2713 253
Size: 795 226 342 395 3235
Fragments arranged by size:
3235 795 395 342 226 165 161 99
93 80 66 29 9
BamHI G'GATC_C
Cuts at: 2718 2718
Size: 5695
BanI G'GyrC_C
Cuts at: 2168 2709 3123 3371 3406 2168
Size: 541 414 248 35 4457
Fragments arranged by size:
4457 541 414 248 35
BanII G_rGCy'C
Cuts at: 340 614 735 890 896 1358 2045 2707 3737
Size: 274 121 155 6 462 687 662 1030
Cuts at: 3737 340
Size: 2298
Fragments arranged by size:
2298 1030 687 662 462 274 155 121
6
BbeI G_GCGC'C
Cuts at: 2172 3127 3375 2172
Size: 955 248 4492
Fragments arranged by size:
4492 955 248
BciVI GTATCCnnnnn_n'
Cuts at: 1202 3591 4354 5695 1202
Size: 2389 763 1341 1202
Fragments arranged by size:
2389 1341 1202 763
BclI T'GATC_A
Cuts at: 3213 3213
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 163 538 701 1266 1734 2034 2323 2503 2524
Size: 375 163 565 468 300 289 180 21
Cuts at: 2524 163
Size: 3334
Fragments arranged by size:
3334 565 468 375 300 289 180 163
21
BglII A'GATC_T
Cuts at: 3208 5247 3208
Size: 2039 3656
BlpI GC'TnA_GC
Cuts at: 691 2805 691
Size: 2114 3581
Bme1580I G_kGCm'C
Cuts at: 340 890 1358 1708 2211 3318 3411 4469 340
Size: 550 468 350 503 1107 93 1058 1566
Fragments arranged by size:
1566 1107 1058 550 503 468 350 93
BmrI ACTGGGnnnn_n'
Cuts at: 312 1550 2905 2986 3321 5467 312
Size: 1238 1355 81 335 2146 540
Fragments arranged by size:
2146 1355 1238 540 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 882 2370 4060 882
Size: 1488 1690 2517
Fragments arranged by size:
2517 1690 1488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 1655 1925 2203 2242 2633 852
Size: 803 270 278 39 391 3914
Fragments arranged by size:
3914 803 391 278 270 39
Bpu10I CC'TnA_GC
Cuts at: 1401 1401
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2869 3748 4242 4540 2869
Size: 879 494 298 4024
Fragments arranged by size:
4024 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1240 1245 5399 1240
Size: 5 4154 1536
Fragments arranged by size:
4154 1536 5
BsaAI yAC'GTr
Cuts at: 312 2421 2685 3046 312
Size: 2109 264 361 2961
Fragments arranged by size:
2961 2109 361 264
BsaBI GATnn'nnATC
Cuts at: 6 3207 5246 6
Size: 3201 2039 455
Fragments arranged by size:
3201 2039 455
BsaHI Gr'CG_yC
Cuts at: 592 2169 2399 2579 3124 3372 4076 5572 592
Size: 1577 230 180 545 248 704 1496 715
Fragments arranged by size:
1577 1496 715 704 545 248 230 180
BsaWI w'CCGG_w
Cuts at: 2469 2882 3403 4083 4357 4504 4915 2469
Size: 413 521 680 274 147 411 3249
Fragments arranged by size:
3249 680 521 413 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 580 610 664 694 973 1003 2360
Size: 30 408 30 54 30 279 30 1357
Cuts at: 2360 2390 2631 2661 142
Size: 30 241 30 3176
Fragments arranged by size:
3176 1357 408 279 241 54 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1612 1768 1924 1964 1996 2407 2491 1612
Size: 156 156 40 32 411 84 4816
Fragments arranged by size:
4816 411 156 156 84 40 32
BseYI C'CCAG_C
Cuts at: 520 651 1359 1635 1811 1816 1958 2280 2528
Size: 131 708 276 176 5 142 322 248
Cuts at: 2528 2617 3118 3876 4455 4951 520
Size: 89 501 758 579 496 1264
Fragments arranged by size:
1264 758 708 579 501 496 322 276
248 176 142 131 89 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2033
Size: 5695
BsiEI CG_ry'CG
Cuts at: 104 153 485 756 3235 3281 4491 104
Size: 49 332 271 2479 46 1210 1308
Fragments arranged by size:
2479 1308 1210 332 271 49 46
BsiHKAI G_wGCw'C
Cuts at: 584 614 2420 2466 2707 3485 3675 4469 584
Size: 30 1806 46 241 778 190 794 1810
Fragments arranged by size:
1810 1806 794 778 241 190 46 30
BsmI GAATG_Cn'
Cuts at: 853 853
Size: 5695
BsmBI CGTCTCn'nnnn_
Cuts at: 583 952 2618 583
Size: 369 1666 3660
Fragments arranged by size:
3660 1666 369
Bsp1286I G_dGCh'C
Cuts at: 340 584 614 735 890 896 1358 1708 2045
Size: 244 30 121 155 6 462 350 337
Cuts at: 2045 2211 2420 2466 2707 3318 3411 3485 3675
Size: 166 209 46 241 611 93 74 190
Cuts at: 3675 3737 4469 340
Size: 62 732 1566
Fragments arranged by size:
1566 732 611 462 350 337 244 241
209 190 166 155 121 93 74 62
46 30 6
BspEI T'CCGG_A
Cuts at: 4915 4915
Size: 5695
BspHI T'CATG_A
Cuts at: 4871 4871
Size: 5695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1258 2743 2957 3259 3640 1258
Size: 1485 214 302 381 3313
Fragments arranged by size:
3313 1485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1446 2136 2226 2436 3985 4039 5525 5690
Size: 1396 690 90 210 1549 54 1486 165
Cuts at: 5690 50
Size: 55
Fragments arranged by size:
1549 1486 1396 690 210 165 90 55
54
BsrDI GCAATG_nn'
Cuts at: 1668 1886 3605 1668
Size: 218 1719 3758
Fragments arranged by size:
3758 1719 218
BsrFI r'CCGG_y
Cuts at: 528 717 751 837 1968 2234 2469 3691 3872
Size: 189 34 86 1131 266 235 1222 181
Cuts at: 3872 4083 528
Size: 211 2140
Fragments arranged by size:
2140 1222 1131 266 235 211 189 181
86 34
BssHII G'CGCG_C
Cuts at: 207 566 721 2296 207
Size: 359 155 1575 3606
Fragments arranged by size:
3606 1575 359 155
BssSI C'ACGA_G
Cuts at: 937 3964 4324 937
Size: 3027 360 2308
Fragments arranged by size:
3027 2308 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3070 3070
Size: 5695
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5695
BstXI CCAn_nnnn'nTGG
Cuts at: 984 1390 1414 2032 984
Size: 406 24 618 4647
Fragments arranged by size:
4647 618 406 24
BstYI r'GATC_y
Cuts at: 1 1782 2718 3185 3208 3543 3789 4803 4889
Size: 1781 936 467 23 335 246 1014 86
Cuts at: 4889 4901 5247 1
Size: 12 346 449
Fragments arranged by size:
1781 1014 936 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2213 2213
Size: 5695
BtgI C'CryG_G
Cuts at: 125 373 386 497 125
Size: 248 13 111 5323
Fragments arranged by size:
5323 248 111 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3643 3824 3987 3643
Size: 181 163 5351
Fragments arranged by size:
5351 181 163
BtsI GCAGTG_nn'
Cuts at: 408 2198 2936 408
Size: 1790 738 3167
Fragments arranged by size:
3167 1790 738
DraI TTT'AAA
Cuts at: 2692 2692
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2016 2016
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 3399 4259 3399
Size: 860 4835
EaeI y'GGCC_r
Cuts at: 482 753 1416 1710 1772 2087 2243 2598 3278
Size: 271 663 294 62 315 156 355 680
Cuts at: 3278 3452 3843 3870 5217 482
Size: 174 391 27 1347 960
Fragments arranged by size:
1347 960 680 663 391 355 315 294
271 174 156 62 27
EagI C'GGCC_G
Cuts at: 482 753 3278 482
Size: 271 2525 2899
Fragments arranged by size:
2899 2525 271
EarI CTCTTCn'nnn_
Cuts at: 1295 2675 3716 3926 1295
Size: 1380 1041 210 3064
Fragments arranged by size:
3064 1380 1041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 505 672 2545 3901 4213 4359 5505 505
Size: 167 1873 1356 312 146 1146 695
Fragments arranged by size:
1873 1356 1146 695 312 167 146
EcoICRI GAG'CTC
Cuts at: 612 2705 612
Size: 2093 3602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 852 1002 1031 1655 1925 2095 2203 2242
Size: 308 150 29 624 270 170 108 39
Cuts at: 2242 2431 2548 2633 3518 3950 4699 4967 544
Size: 189 117 85 885 432 749 268 1272
Fragments arranged by size:
1272 885 749 624 432 308 270 268
189 170 150 117 108 85 39 29
EcoNI CCTnn'n_nnAGG
Cuts at: 856 1300 2563 856
Size: 444 1263 3988
Fragments arranged by size:
3988 1263 444
EcoO109I rG'GnC_Cy
Cuts at: 132 139 337 423 431 760 995 1305 1354
Size: 7 198 86 8 329 235 310 49
Cuts at: 1354 1355 1675 1937 2115 2639 2832 132
Size: 1 320 262 178 524 193 2995
Fragments arranged by size:
2995 524 329 320 310 262 235 198
193 178 86 49 8 7 1
EcoRI G'AATT_C
Cuts at: 2697 2697
Size: 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 807 3712 807
Size: 2905 2790
FspI TGC'GCA
Cuts at: 3474 3474
Size: 5695
HaeII r_GCGC'y
Cuts at: 919 1046 2172 3127 3375 4399 5378 919
Size: 127 1126 955 248 1024 979 1236
Fragments arranged by size:
1236 1126 1024 979 955 248 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 979 1011 1578 1610 1921 1953 2360
Size: 32 805 32 567 32 311 32 407
Cuts at: 2360 2392 2631 2663 3535 3565 3567 3597 142
Size: 32 239 32 872 30 2 30 2240
Fragments arranged by size:
2240 872 805 567 407 311 239 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2732 2732
Size: 5695
KasI G'GCGC_C
Cuts at: 2168 3123 3371 2168
Size: 955 248 4492
Fragments arranged by size:
4492 955 248
KpnI G_GTAC'C
Cuts at: 2713 2713
Size: 5695
MluI A'CGCG_T
Cuts at: 5128 5128
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 924 1012 1018 4366 4550 924
Size: 88 6 3348 184 2069
Fragments arranged by size:
3348 2069 184 88 6
MscI TGG'CCA
Cuts at: 1418 1712 1774 2600 3454 1418
Size: 294 62 826 854 3659
Fragments arranged by size:
3659 854 826 294 62
MslI CAynn'nnrTG
Cuts at: 2157 3682 3809 2157
Size: 1525 127 4043
Fragments arranged by size:
4043 1525 127
MspA1I CmG'CkG
Cuts at: 375 388 465 499 555 573 780 1606 1639
Size: 13 77 34 56 18 207 826 33
Cuts at: 1639 2385 2804 3118 3478 4493 4738 375
Size: 746 419 314 360 1015 245 1332
Fragments arranged by size:
1332 1015 826 746 419 360 314 245
207 77 56 34 33 18 13
NaeI GCC'GGC
Cuts at: 530 719 753 839 1970 3874 530
Size: 189 34 86 1131 1904 2351
Fragments arranged by size:
2351 1904 1131 189 86 34
NarI GG'CG_CC
Cuts at: 2169 3124 3372 2169
Size: 955 248 4492
Fragments arranged by size:
4492 955 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5695
NgoMIV G'CCGG_C
Cuts at: 528 717 751 837 1968 3872 528
Size: 189 34 86 1131 1904 2351
Fragments arranged by size:
2351 1904 1131 189 86 34
NruI TCG'CGA
Cuts at: 4056 4056
Size: 5695
NspI r_CATG'y
Cuts at: 2746 3246 4155 2746
Size: 500 909 4286
Fragments arranged by size:
4286 909 500
PasI CC'CwG_GG
Cuts at: 135 883 1350 1833 2635 135
Size: 748 467 483 802 3195
Fragments arranged by size:
3195 802 748 483 467
PciI A'CATG_T
Cuts at: 4151 4151
Size: 5695
PfoI T'CCnGG_A
Cuts at: 584 1458 584
Size: 874 4821
PmeI GTTT'AAAC
Cuts at: 2692 2692
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 580 612 973 1005 4834 4866 580
Size: 32 361 32 3829 32 1409
Fragments arranged by size:
3829 1409 361 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 139 423 431 760 995 1675 1937 2115 2639
Size: 284 8 329 235 680 262 178 524
Cuts at: 2639 139
Size: 3195
Fragments arranged by size:
3195 680 524 329 284 262 235 178
8
PspOMI G'GGCC_C
Cuts at: 336 886 1354 336
Size: 550 468 4677
Fragments arranged by size:
4677 550 468
PspXI vC'TCGA_Gb
Cuts at: 534 1750 534
Size: 1216 4479
PstI C_TGCA'G
Cuts at: 1200 1470 1611 2144 2193 2366 2384 2740 3425
Size: 270 141 533 49 173 18 356 685
Cuts at: 3425 1200
Size: 3470
Fragments arranged by size:
3470 685 533 356 270 173 141 49
18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5695
PvuII CAG'CTG
Cuts at: 1606 1639 3118 3478 1606
Size: 33 1479 360 3823
Fragments arranged by size:
3823 1479 360 33
RsrII CG'GwC_CG
Cuts at: 3888 3888
Size: 5695
SacI G_AGCT'C
Cuts at: 614 2707 614
Size: 2093 3602
SacII CC_GC'GG
Cuts at: 376 389 500 376
Size: 13 111 5571
Fragments arranged by size:
5571 111 13
SalI G'TCGA_C
Cuts at: 2730 2730
Size: 5695
SanDI GG'GwC_CC
Cuts at: 431 760 431
Size: 329 5366
SapI GCTCTTCn'nnn_
Cuts at: 3716 3926 3716
Size: 210 5485
SbfI CC_TGCA'GG
Cuts at: 2740 2740
Size: 5695
ScaI AGT'ACT
Cuts at: 1923 5212 1923
Size: 3289 2406
SfcI C'TryA_G
Cuts at: 33 222 1196 1466 1505 1607 2140 2189 2362
Size: 189 974 270 39 102 533 49 173
Cuts at: 2362 2380 2736 3421 4416 4607 33
Size: 18 356 685 995 191 1121
Fragments arranged by size:
1121 995 974 685 533 356 270 191
189 173 102 49 39 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 538 1734 2323 538
Size: 1196 589 3910
Fragments arranged by size:
3910 1196 589
SfoI GGC'GCC
Cuts at: 2170 3125 3373 2170
Size: 955 248 4492
Fragments arranged by size:
4492 955 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5695
SmaI CCC'GGG
Cuts at: 335 428 437 701 730 2320 2715 335
Size: 93 9 264 29 1590 395 3315
Fragments arranged by size:
3315 1590 395 264 93 29 9
SmlI C'TyrA_G
Cuts at: 534 1750 2848 3763 4257 4519 534
Size: 1216 1098 915 494 262 1710
Fragments arranged by size:
1710 1216 1098 915 494 262
SnaBI TAC'GTA
Cuts at: 2685 2685
Size: 5695
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5695
SphI G_CATG'C
Cuts at: 2746 3246 2746
Size: 500 5195
SrfI GCCC'GGGC
Cuts at: 335 2320 335
Size: 1985 3710
StuI AGG'CCT
Cuts at: 862 2203 2353 862
Size: 1341 150 4204
Fragments arranged by size:
4204 1341 150
StyI C'CwwG_G
Cuts at: 125 896 969 1113 1646 2402 2595 2827 3181
Size: 771 73 144 533 756 193 232 354
Cuts at: 3181 125
Size: 2639
Fragments arranged by size:
2639 771 756 533 354 232 193 144
73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 844 1972 2023 3276 3409 3868 4079 844
Size: 1128 51 1253 133 459 211 2460
Fragments arranged by size:
2460 1253 1128 459 211 133 51
TatI w'GTAC_w
Cuts at: 1921 5210 1921
Size: 3289 2406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 664 696 5212 5244 664
Size: 32 4516 32 1115
Fragments arranged by size:
4516 1115 32 32
Tth111I GACn'n_nGTC
Cuts at: 3490 3490
Size: 5695
XbaI T'CTAG_A
Cuts at: 2724 2724
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 180 1537 3188 132
Size: 48 1357 1651 2639
Fragments arranged by size:
2639 1651 1357 48
XhoI C'TCGA_G
Cuts at: 534 1750 534
Size: 1216 4479
XmaI C'CCGG_G
Cuts at: 333 426 435 699 728 2318 2713 333
Size: 93 9 264 29 1590 395 3315
Fragments arranged by size:
3315 1590 395 264 93 29 9
ZraI GAC'GTC
Cuts at: 593 593
Size: 5695
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BmgBI BmtI BsiWI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PflMI PmlI PshAI PsiI
PsrI SexAI SgrAI SspI SwaI XmnI