Restriction map for
pF1KSDA1950
(Circular) (Six-base) MAPSORT of: pF1KSDA1950.seq Check: 7623 from: 1 to: 4631
pF1KSDA1950 4631 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1401 1401
Size: 4631
AccI GT'mk_AC
Cuts at: 138 1669 138
Size: 1531 3100
Acc65I G'GTAC_C
Cuts at: 1067 1647 1067
Size: 580 4051
AclI AA'CG_TT
Cuts at: 4471 4471
Size: 4631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 849 2456 2888 3635 3903 687
Size: 162 1607 432 747 268 1415
Fragments arranged by size:
1607 1415 747 432 268 162
AflIII A'CryG_T
Cuts at: 1983 3087 4064 1983
Size: 1104 977 2550
Fragments arranged by size:
2550 1104 977
AgeI A'CCGG_T
Cuts at: 3019 3019
Size: 4631
AleI CACnn'nnGTG
Cuts at: 1213 1213
Size: 4631
AlwNI CAG_nnn'CTG
Cuts at: 216 851 1377 1936 3503 216
Size: 635 526 559 1567 1344
Fragments arranged by size:
1567 1344 635 559 526
ApaI G_GGCC'C
Cuts at: 167 782 858 1324 167
Size: 615 76 466 3474
Fragments arranged by size:
3474 615 466 76
ApaLI G'TGCA_C
Cuts at: 3401 3401
Size: 4631
ApoI r'AATT_y
Cuts at: 763 1635 3984 4240 763
Size: 872 2349 256 1154
Fragments arranged by size:
2349 1154 872 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4631
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4631
AvaI C'yCGr_G
Cuts at: 167 337 346 826 1173 1651 167
Size: 170 9 480 347 478 3147
Fragments arranged by size:
3147 480 478 347 170 9
BamHI G'GATC_C
Cuts at: 1656 1656
Size: 4631
BanI G'GyrC_C
Cuts at: 1067 1207 1647 2061 2309 2344 1067
Size: 140 440 414 248 35 3354
Fragments arranged by size:
3354 440 414 248 140 35
BanII G_rGCy'C
Cuts at: 167 782 858 914 1205 1226 1324 1645 2675
Size: 615 76 56 291 21 98 321 1030
Cuts at: 2675 167
Size: 2123
Fragments arranged by size:
2123 1030 615 321 291 98 76 56
21
BbeI G_GCGC'C
Cuts at: 2065 2313 2065
Size: 248 4383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 297 331 297
Size: 34 4597
BciVI GTATCCnnnnn_n'
Cuts at: 2529 3290 4631 2529
Size: 761 1341 2529
Fragments arranged by size:
2529 1341 761
BclI T'GATC_A
Cuts at: 2151 2151
Size: 4631
BglI GCCn_nnn'nGGC
Cuts at: 1300 1300
Size: 4631
BglII A'GATC_T
Cuts at: 2146 4183 2146
Size: 2037 2594
BlpI GC'TnA_GC
Cuts at: 973 1279 1743 973
Size: 306 464 3861
Fragments arranged by size:
3861 464 306
Bme1580I G_kGCm'C
Cuts at: 167 725 782 858 873 1324 1566 2256 2349
Size: 558 57 76 15 451 242 690 93
Cuts at: 2349 3405 167
Size: 1056 1393
Fragments arranged by size:
1393 1056 690 558 451 242 93 76
57 15
BmgBI CAC'GTC
Cuts at: 1109 1109
Size: 4631
BmrI ACTGGGnnnn_n'
Cuts at: 1843 1924 2259 4403 1843
Size: 81 335 2144 2071
Fragments arranged by size:
2144 2071 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1212 2998 1212
Size: 1786 2845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 711 568
Size: 143 4488
Bpu10I CC'TnA_GC
Cuts at: 328 433 328
Size: 105 4526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1807 2686 3178 3476 1807
Size: 879 492 298 2962
Fragments arranged by size:
2962 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 195 4335 195
Size: 4140 491
BsaAI yAC'GTr
Cuts at: 902 1623 1984 902
Size: 721 361 3549
Fragments arranged by size:
3549 721 361
BsaBI GATnn'nnATC
Cuts at: 6 2145 4182 6
Size: 2139 2037 455
Fragments arranged by size:
2139 2037 455
BsaHI Gr'CG_yC
Cuts at: 734 1430 2062 2310 3012 4508 734
Size: 696 632 248 702 1496 857
Fragments arranged by size:
1496 857 702 696 632 248
BsaWI w'CCGG_w
Cuts at: 383 1820 2341 3019 3293 3440 3851 383
Size: 1437 521 678 274 147 411 1163
Fragments arranged by size:
1437 1163 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1522 1552 1522
Size: 30 4601
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 543 1411 1530 1563 454
Size: 89 868 119 33 3522
Fragments arranged by size:
3522 868 119 89 33
BseYI C'CCAG_C
Cuts at: 562 651 781 851 1114 1442 1489 1524 2056
Size: 89 130 70 263 328 47 35 532
Cuts at: 2056 2814 3391 3887 562
Size: 758 577 496 1306
Fragments arranged by size:
1306 758 577 532 496 328 263 130
89 70 47 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 448 465 495 1429 276
Size: 172 17 30 934 3478
Fragments arranged by size:
3478 934 172 30 17
BsiEI CG_ry'CG
Cuts at: 104 1440 2173 2219 3427 104
Size: 1336 733 46 1208 1308
Fragments arranged by size:
1336 1308 1208 733 46
BsiHKAI G_wGCw'C
Cuts at: 914 1645 2423 2613 3405 914
Size: 731 778 190 792 2140
Fragments arranged by size:
2140 792 778 731 190
Bsp1286I G_dGCh'C
Cuts at: 167 725 782 858 873 914 1205 1226 1324
Size: 558 57 76 15 41 291 21 98
Cuts at: 1324 1566 1645 2256 2349 2423 2613 2675 3405
Size: 242 79 611 93 74 190 62 730
Cuts at: 3405 167
Size: 1393
Fragments arranged by size:
1393 730 611 558 291 242 190 98
93 79 76 74 62 57 41 21
15
BspEI T'CCGG_A
Cuts at: 3851 3851
Size: 4631
BspHI T'CATG_A
Cuts at: 3807 3807
Size: 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1401 1681 1895 2197 2578 1401
Size: 280 214 302 381 3454
Fragments arranged by size:
3454 381 302 280 214
BsrBI CCG'CTC
Cuts at: 50 246 2923 2977 4461 4626 50
Size: 196 2677 54 1484 165 55
Fragments arranged by size:
2677 1484 196 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2543 2543
Size: 4631
BsrFI r'CCGG_y
Cuts at: 444 2629 2810 3019 444
Size: 2185 181 209 2056
Fragments arranged by size:
2185 2056 209 181
BssSI C'ACGA_G
Cuts at: 907 2902 3260 907
Size: 1995 358 2278
Fragments arranged by size:
2278 1995 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2008 2008
Size: 4631
BstBI TT'CG_AA
Cuts at: 110 2992 110
Size: 2882 1749
BstYI r'GATC_y
Cuts at: 1 1656 2123 2146 2481 2727 3739 3825 3837
Size: 1655 467 23 335 246 1012 86 12
Cuts at: 3837 4183 1
Size: 346 449
Fragments arranged by size:
1655 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1024 1260 1546 1024
Size: 236 286 4109
Fragments arranged by size:
4109 286 236
BtgI C'CryG_G
Cuts at: 1424 1424
Size: 4631
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2581 2762 2925 2581
Size: 181 163 4287
Fragments arranged by size:
4287 181 163
BtsI GCAGTG_nn'
Cuts at: 139 1874 139
Size: 1735 2896
DraI TTT'AAA
Cuts at: 1630 1630
Size: 4631
DraIII CAC_nnn'GTG
Cuts at: 729 729
Size: 4631
DrdI GACnn_nn'nnGTC
Cuts at: 187 2337 3195 187
Size: 2150 858 1623
Fragments arranged by size:
2150 1623 858
EaeI y'GGCC_r
Cuts at: 257 1358 1469 2216 2390 2781 2808 4153 257
Size: 1101 111 747 174 391 27 1345 735
Fragments arranged by size:
1345 1101 747 735 391 174 111 27
EagI C'GGCC_G
Cuts at: 2216 2216
Size: 4631
EarI CTCTTCn'nnn_
Cuts at: 256 292 1607 2654 2864 256
Size: 36 1315 1047 210 2023
Fragments arranged by size:
2023 1315 1047 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 216 2839 3149 3295 4441 216
Size: 2623 310 146 1146 406
Fragments arranged by size:
2623 1146 406 310 146
EcoICRI GAG'CTC
Cuts at: 912 1643 912
Size: 731 3900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 687 711 849 2456 2888 3635 3903 568
Size: 119 24 138 1607 432 747 268 1296
Fragments arranged by size:
1607 1296 747 432 268 138 119 24
EcoNI CCTnn'n_nnAGG
Cuts at: 259 436 805 961 982 1408 1545 259
Size: 177 369 156 21 426 137 3345
Fragments arranged by size:
3345 426 369 177 156 137 21
EcoO109I rG'GnC_Cy
Cuts at: 164 778 831 855 987 1320 1321 1374 1401
Size: 614 53 24 132 333 1 53 27
Cuts at: 1401 1482 1770 164
Size: 81 288 3025
Fragments arranged by size:
3025 614 333 288 132 81 53 53
27 24 1
EcoRI G'AATT_C
Cuts at: 763 1635 763
Size: 872 3759
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4631
FspI TGC'GCA
Cuts at: 1516 2412 1516
Size: 896 3735
HaeII r_GCGC'y
Cuts at: 788 1169 2065 2313 3335 4314 788
Size: 381 896 248 1022 979 1105
Fragments arranged by size:
1105 1022 979 896 381 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1522 1554 2473 2503 2505 2535 1522
Size: 32 919 30 2 30 3618
Fragments arranged by size:
3618 919 32 30 30 2
HincII GTy'rAC
Cuts at: 1670 1670
Size: 4631
KasI G'GCGC_C
Cuts at: 2061 2309 2061
Size: 248 4383
KpnI G_GTAC'C
Cuts at: 1071 1651 1071
Size: 580 4051
MluI A'CGCG_T
Cuts at: 4064 4064
Size: 4631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1199 3302 3486 1199
Size: 2103 184 2344
Fragments arranged by size:
2344 2103 184
MscI TGG'CCA
Cuts at: 1360 2392 1360
Size: 1032 3599
MslI CAynn'nnrTG
Cuts at: 1213 2620 2747 1213
Size: 1407 127 3097
Fragments arranged by size:
3097 1407 127
MspA1I CmG'CkG
Cuts at: 216 471 479 548 944 953 1308 1383 1442
Size: 255 8 69 396 9 355 75 59
Cuts at: 1442 1742 2056 2416 3429 3674 216
Size: 300 314 360 1013 245 1173
Fragments arranged by size:
1173 1013 396 360 355 314 300 255
245 75 69 59 9 8
NaeI GCC'GGC
Cuts at: 2812 2812
Size: 4631
NarI GG'CG_CC
Cuts at: 2062 2310 2062
Size: 248 4383
NgoMIV G'CCGG_C
Cuts at: 2810 2810
Size: 4631
NspI r_CATG'y
Cuts at: 1042 1182 1684 2184 3091 1042
Size: 140 502 500 907 2582
Fragments arranged by size:
2582 907 502 500 140
PasI CC'CwG_GG
Cuts at: 873 1601 873
Size: 728 3903
PciI A'CATG_T
Cuts at: 3087 3087
Size: 4631
PflMI CCAn_nnn'nTGG
Cuts at: 924 924
Size: 4631
PmeI GTTT'AAAC
Cuts at: 1630 1630
Size: 4631
PmlI CAC'GTG
Cuts at: 902 902
Size: 4631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 4599
PpuMI rG'GwC_Cy
Cuts at: 1401 1482 1401
Size: 81 4550
PshAI GACnn'nnGTC
Cuts at: 1010 1010
Size: 4631
PspOMI G'GGCC_C
Cuts at: 163 778 854 1320 163
Size: 615 76 466 3474
Fragments arranged by size:
3474 615 466 76
PstI C_TGCA'G
Cuts at: 134 212 380 742 842 1037 1382 1678 2363
Size: 78 168 362 100 195 345 296 685
Cuts at: 2363 134
Size: 2402
Fragments arranged by size:
2402 685 362 345 296 195 168 100
78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4631
PvuII CAG'CTG
Cuts at: 216 471 479 2056 2416 216
Size: 255 8 1577 360 2431
Fragments arranged by size:
2431 1577 360 255 8
RsrII CG'GwC_CG
Cuts at: 2826 2826
Size: 4631
SacI G_AGCT'C
Cuts at: 914 1645 914
Size: 731 3900
SalI G'TCGA_C
Cuts at: 1668 1668
Size: 4631
SapI GCTCTTCn'nnn_
Cuts at: 256 292 2654 2864 256
Size: 36 2362 210 2023
Fragments arranged by size:
2362 2023 210 36
SbfI CC_TGCA'GG
Cuts at: 842 1678 842
Size: 836 3795
ScaI AGT'ACT
Cuts at: 4148 4148
Size: 4631
SexAI A'CCwGG_T
Cuts at: 1403 1403
Size: 4631
SfcI C'TryA_G
Cuts at: 33 130 208 376 418 738 838 1033 1378
Size: 97 78 168 42 320 100 195 345
Cuts at: 1378 1674 2359 3352 3543 33
Size: 296 685 993 191 1121
Fragments arranged by size:
1121 993 685 345 320 296 195 191
168 100 97 78 42
SfoI GGC'GCC
Cuts at: 2063 2311 2063
Size: 248 4383
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4631
SmaI CCC'GGG
Cuts at: 169 1653 169
Size: 1484 3147
SmlI C'TyrA_G
Cuts at: 1786 2701 3193 3455 1786
Size: 915 492 262 2962
Fragments arranged by size:
2962 915 492 262
SnaBI TAC'GTA
Cuts at: 1623 1623
Size: 4631
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4631
SphI G_CATG'C
Cuts at: 1684 2184 1684
Size: 500 4131
StuI AGG'CCT
Cuts at: 265 490 688 265
Size: 225 198 4208
Fragments arranged by size:
4208 225 198
StyI C'CwwG_G
Cuts at: 1765 2119 1765
Size: 354 4277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 2214 2347 2806 3015 1503
Size: 711 133 459 209 3119
Fragments arranged by size:
3119 711 459 209 133
TatI w'GTAC_w
Cuts at: 4146 4146
Size: 4631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 628 660 1545 1577 4148 4180 628
Size: 32 885 32 2571 32 1079
Fragments arranged by size:
2571 1079 885 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2428 2428
Size: 4631
XbaI T'CTAG_A
Cuts at: 1662 1662
Size: 4631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2126 2126
Size: 4631
XmaI C'CCGG_G
Cuts at: 167 1651 167
Size: 1484 3147
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI