Restriction map for
pF1KSDA1932
(Circular) (Six-base) MAPSORT of: pF1KSDA1932.seq Check: 6054 from: 1 to: 5474
pF1KSDA1932 5474 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1681 1681
Size: 5474
AccI GT'mk_AC
Cuts at: 2512 2512
Size: 5474
Acc65I G'GTAC_C
Cuts at: 392 2490 392
Size: 2098 3376
AclI AA'CG_TT
Cuts at: 5314 5314
Size: 5474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2104 3299 3731 4478 4746 1555
Size: 549 1195 432 747 268 2283
Fragments arranged by size:
2283 1195 747 549 432 268
AfeI AGC'GCT
Cuts at: 910 910
Size: 5474
AflIII A'CryG_T
Cuts at: 341 1802 2826 3930 4907 341
Size: 1461 1024 1104 977 908
Fragments arranged by size:
1461 1104 1024 977 908
AgeI A'CCGG_T
Cuts at: 3862 3862
Size: 5474
AhdI GACnn_n'nnGTC
Cuts at: 710 710
Size: 5474
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2112 2112
Size: 5474
AlwNI CAG_nnn'CTG
Cuts at: 261 286 495 1656 1842 2779 4346 261
Size: 25 209 1161 186 937 1567 1389
Fragments arranged by size:
1567 1389 1161 937 209 186 25
ApaI G_GGCC'C
Cuts at: 608 1419 2136 608
Size: 811 717 3946
Fragments arranged by size:
3946 811 717
ApaLI G'TGCA_C
Cuts at: 332 4244 332
Size: 3912 1562
ApoI r'AATT_y
Cuts at: 2478 4827 5083 2478
Size: 2349 256 2869
Fragments arranged by size:
2869 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5474
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
AvaI C'yCGr_G
Cuts at: 848 1046 1168 1193 1591 2040 2494 848
Size: 198 122 25 398 449 454 3828
Fragments arranged by size:
3828 454 449 398 198 122 25
BamHI G'GATC_C
Cuts at: 2499 2499
Size: 5474
BanI G'GyrC_C
Cuts at: 392 1144 1180 1470 1669 2262 2490 2904 3152
Size: 752 36 290 199 593 228 414 248
Cuts at: 3152 3187 392
Size: 35 2679
Fragments arranged by size:
2679 752 593 414 290 248 228 199
36 35
BanII G_rGCy'C
Cuts at: 543 608 1031 1069 1194 1419 1598 2136 2488
Size: 65 423 38 125 225 179 538 352
Cuts at: 2488 3518 543
Size: 1030 2499
Fragments arranged by size:
2499 1030 538 423 352 225 179 125
65 38
BbeI G_GCGC'C
Cuts at: 1474 2908 3156 1474
Size: 1434 248 3792
Fragments arranged by size:
3792 1434 248
BbsI GAAGACnn'nnnn_
Cuts at: 158 1224 158
Size: 1066 4408
BbvCI CC'TCA_GC
Cuts at: 360 488 515 563 360
Size: 128 27 48 5271
Fragments arranged by size:
5271 128 48 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1876 1910 1984 2018 1876
Size: 34 74 34 5332
Fragments arranged by size:
5332 74 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3372 4133 5474 3372
Size: 761 1341 3372
Fragments arranged by size:
3372 1341 761
BclI T'GATC_A
Cuts at: 144 2246 2994 144
Size: 2102 748 2624
Fragments arranged by size:
2624 2102 748
BglI GCCn_nnn'nGGC
Cuts at: 256 2239 256
Size: 1983 3491
BglII A'GATC_T
Cuts at: 2989 5026 2989
Size: 2037 3437
BlpI GC'TnA_GC
Cuts at: 506 2586 506
Size: 2080 3394
Bme1580I G_kGCm'C
Cuts at: 336 608 1149 1419 1672 1716 2136 3099 3192
Size: 272 541 270 253 44 420 963 93
Cuts at: 3192 4248 336
Size: 1056 1562
Fragments arranged by size:
1562 1056 963 541 420 272 270 253
93 44
BmrI ACTGGGnnnn_n'
Cuts at: 439 2350 2686 2767 3102 5246 439
Size: 1911 336 81 335 2144 667
Fragments arranged by size:
2144 1911 667 336 335 81
BmtI G_CTAG'C
Cuts at: 1097 1097
Size: 5474
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3841 3841
Size: 5474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 728 1038 1272 1528 1642 563
Size: 165 310 234 256 114 4395
Fragments arranged by size:
4395 310 256 234 165 114
Bpu10I CC'TnA_GC
Cuts at: 360 488 515 563 360
Size: 128 27 48 5271
Fragments arranged by size:
5271 128 48 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 949 1939 2221 2295 2650 3529 4021 4319
Size: 249 990 282 74 355 879 492 298
Cuts at: 4319 700
Size: 1855
Fragments arranged by size:
1855 990 879 492 355 298 282 249
74
BsaI GGTCTCn'nnnn_
Cuts at: 718 1882 5178 718
Size: 1164 3296 1014
Fragments arranged by size:
3296 1164 1014
BsaAI yAC'GTr
Cuts at: 342 2466 2827 342
Size: 2124 361 2989
Fragments arranged by size:
2989 2124 361
BsaBI GATnn'nnATC
Cuts at: 6 2988 5025 6
Size: 2982 2037 455
Fragments arranged by size:
2982 2037 455
BsaHI Gr'CG_yC
Cuts at: 217 1165 1471 2905 3153 3855 5351 217
Size: 948 306 1434 248 702 1496 340
Fragments arranged by size:
1496 1434 948 702 340 306 248
BsaWI w'CCGG_w
Cuts at: 2663 3184 3862 4136 4283 4694 2663
Size: 521 678 274 147 411 3443
Fragments arranged by size:
3443 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1258 1288 1318 1348 1558 1588 1258
Size: 30 30 30 210 30 5144
Fragments arranged by size:
5144 210 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 949 1272 1275 2332 949
Size: 323 3 1057 4091
Fragments arranged by size:
4091 1057 323 3
BseYI C'CCAG_C
Cuts at: 187 803 1154 1174 2899 3657 4234 4730 187
Size: 616 351 20 1725 758 577 496 931
Fragments arranged by size:
1725 931 758 616 577 496 351 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 1870
Size: 5474
BsiEI CG_ry'CG
Cuts at: 104 1188 1410 3016 3062 4270 104
Size: 1084 222 1606 46 1208 1308
Fragments arranged by size:
1606 1308 1208 1084 222 46
BsiHKAI G_wGCw'C
Cuts at: 336 2488 3266 3456 4248 336
Size: 2152 778 190 792 1562
Fragments arranged by size:
2152 1562 792 778 190
BsmI GAATG_Cn'
Cuts at: 2404 2404
Size: 5474
BsmBI CGTCTCn'nnnn_
Cuts at: 208 2313 208
Size: 2105 3369
Bsp1286I G_dGCh'C
Cuts at: 336 543 608 1031 1069 1149 1194 1419 1598
Size: 207 65 423 38 80 45 225 179
Cuts at: 1598 1672 1716 2136 2488 3099 3192 3266 3456
Size: 74 44 420 352 611 93 74 190
Cuts at: 3456 3518 4248 336
Size: 62 730 1562
Fragments arranged by size:
1562 730 611 423 420 352 225 207
190 179 93 80 74 74 65 62
45 44 38
BspEI T'CCGG_A
Cuts at: 4694 4694
Size: 5474
BspHI T'CATG_A
Cuts at: 1235 4650 1235
Size: 3415 2059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 657 1681 1706 2524 2738 3040 3421 657
Size: 1024 25 818 214 302 381 2710
Fragments arranged by size:
2710 1024 818 381 302 214 25
BsrBI CCG'CTC
Cuts at: 50 1274 1280 3766 3820 5304 5469 50
Size: 1224 6 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 1224 165 55 54 6
BsrDI GCAATG_nn'
Cuts at: 321 1992 3386 321
Size: 1671 1394 2409
Fragments arranged by size:
2409 1671 1394
BsrFI r'CCGG_y
Cuts at: 289 1183 3472 3653 3862 289
Size: 894 2289 181 209 1901
Fragments arranged by size:
2289 1901 894 209 181
BssSI C'ACGA_G
Cuts at: 226 2075 2197 3745 4103 226
Size: 1849 122 1548 358 1597
Fragments arranged by size:
1849 1597 1548 358 122
BstAPI GCAn_nnn'nTGC
Cuts at: 1656 1698 2851 1656
Size: 42 1153 4279
Fragments arranged by size:
4279 1153 42
BstBI TT'CG_AA
Cuts at: 110 3835 110
Size: 3725 1749
BstEII G'GTnAC_C
Cuts at: 1918 1918
Size: 5474
BstXI CCAn_nnnn'nTGG
Cuts at: 625 763 790 2061 625
Size: 138 27 1271 4038
Fragments arranged by size:
4038 1271 138 27
BstYI r'GATC_y
Cuts at: 1 1839 2091 2499 2966 2989 3324 3570 4582
Size: 1838 252 408 467 23 335 246 1012
Cuts at: 4582 4668 4680 5026 1
Size: 86 12 346 449
Fragments arranged by size:
1838 1012 467 449 408 346 335 252
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1394 1394
Size: 5474
BtgI C'CryG_G
Cuts at: 125 611 2054 125
Size: 486 1443 3545
Fragments arranged by size:
3545 1443 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3424 3605 3768 3424
Size: 181 163 5130
Fragments arranged by size:
5130 181 163
BtsI GCAGTG_nn'
Cuts at: 1635 2717 1635
Size: 1082 4392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 658 693 658
Size: 35 5439
DraI TTT'AAA
Cuts at: 2473 2473
Size: 5474
DraIII CAC_nnn'GTG
Cuts at: 1371 1371
Size: 5474
DrdI GACnn_nn'nnGTC
Cuts at: 3180 4038 3180
Size: 858 4616
EaeI y'GGCC_r
Cuts at: 131 263 326 3059 3233 3624 3651 4996 131
Size: 132 63 2733 174 391 27 1345 609
Fragments arranged by size:
2733 1345 609 391 174 132 63 27
EagI C'GGCC_G
Cuts at: 3059 3059
Size: 5474
EarI CTCTTCn'nnn_
Cuts at: 1257 1450 1922 2293 2457 3497 3707 1257
Size: 193 472 371 164 1040 210 3024
Fragments arranged by size:
3024 1040 472 371 210 193 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 3682 3992 4138 5284 2290
Size: 1392 310 146 1146 2480
Fragments arranged by size:
2480 1392 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2486 2486
Size: 5474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 728 1038 1272 1528 1555 1642 2104 3299
Size: 165 310 234 256 27 87 462 1195
Cuts at: 3299 3731 4478 4746 563
Size: 432 747 268 1291
Fragments arranged by size:
1291 1195 747 462 432 310 268 256
234 165 87 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1112 1287 1112
Size: 175 5299
EcoO109I rG'GnC_Cy
Cuts at: 258 604 839 1292 1415 1518 2133 2613 258
Size: 346 235 453 123 103 615 480 3119
Fragments arranged by size:
3119 615 480 453 346 235 123 103
EcoRI G'AATT_C
Cuts at: 2478 2478
Size: 5474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 756 3493 756
Size: 2737 2737
FspI TGC'GCA
Cuts at: 3255 3255
Size: 5474
HaeII r_GCGC'y
Cuts at: 912 1474 1507 2908 3156 4178 5157 912
Size: 562 33 1401 248 1022 979 1229
Fragments arranged by size:
1401 1229 1022 979 562 248 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 696 728 1234 1258 1266 1290 1312
Size: 32 210 32 506 24 8 24 22
Cuts at: 1312 1344 1449 1481 3316 3346 3348 3378 454
Size: 32 105 32 1835 30 2 30 2550
Fragments arranged by size:
2550 1835 506 210 105 32 32 32
32 30 30 24 24 22 8 2
HincII GTy'rAC
Cuts at: 2513 2513
Size: 5474
KasI G'GCGC_C
Cuts at: 1470 2904 3152 1470
Size: 1434 248 3792
Fragments arranged by size:
3792 1434 248
KpnI G_GTAC'C
Cuts at: 396 2494 396
Size: 2098 3376
MluI A'CGCG_T
Cuts at: 1802 4907 1802
Size: 3105 2369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 911 1361 4145 4329 794
Size: 117 450 2784 184 1939
Fragments arranged by size:
2784 1939 450 184 117
MscI TGG'CCA
Cuts at: 265 328 3235 265
Size: 63 2907 2504
Fragments arranged by size:
2907 2504 63
MslI CAynn'nnrTG
Cuts at: 3463 3590 3463
Size: 127 5347
MspA1I CmG'CkG
Cuts at: 495 675 836 2585 2899 3259 4272 4517 495
Size: 180 161 1749 314 360 1013 245 1452
Fragments arranged by size:
1749 1452 1013 360 314 245 180 161
NaeI GCC'GGC
Cuts at: 291 3655 291
Size: 3364 2110
NarI GG'CG_CC
Cuts at: 1471 2905 3153 1471
Size: 1434 248 3792
Fragments arranged by size:
3792 1434 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5474
NdeI CA'TA_TG
Cuts at: 1421 1421
Size: 5474
NgoMIV G'CCGG_C
Cuts at: 289 3653 289
Size: 3364 2110
NheI G'CTAG_C
Cuts at: 1093 1093
Size: 5474
NspI r_CATG'y
Cuts at: 471 2187 2527 3027 3934 471
Size: 1716 340 500 907 2011
Fragments arranged by size:
2011 1716 907 500 340
PasI CC'CwG_GG
Cuts at: 700 2136 700
Size: 1436 4038
PciI A'CATG_T
Cuts at: 3930 3930
Size: 5474
PflMI CCAn_nnn'nTGG
Cuts at: 1016 2112 1016
Size: 1096 4378
PmeI GTTT'AAAC
Cuts at: 2473 2473
Size: 5474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4613 4645 4613
Size: 32 5442
PpuMI rG'GwC_Cy
Cuts at: 839 1292 1518 839
Size: 453 226 4795
Fragments arranged by size:
4795 453 226
PspOMI G'GGCC_C
Cuts at: 604 1415 2132 604
Size: 811 717 3946
Fragments arranged by size:
3946 811 717
PspXI vC'TCGA_Gb
Cuts at: 1591 1591
Size: 5474
PstI C_TGCA'G
Cuts at: 980 992 1610 1661 2521 3206 980
Size: 12 618 51 860 685 3248
Fragments arranged by size:
3248 860 685 618 51 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5474
PvuII CAG'CTG
Cuts at: 495 675 2899 3259 495
Size: 180 2224 360 2710
Fragments arranged by size:
2710 2224 360 180
RsrII CG'GwC_CG
Cuts at: 3669 3669
Size: 5474
SacI G_AGCT'C
Cuts at: 2488 2488
Size: 5474
SalI G'TCGA_C
Cuts at: 2511 2511
Size: 5474
SapI GCTCTTCn'nnn_
Cuts at: 1257 2293 3497 3707 1257
Size: 1036 1204 210 3024
Fragments arranged by size:
3024 1204 1036 210
SbfI CC_TGCA'GG
Cuts at: 2521 2521
Size: 5474
ScaI AGT'ACT
Cuts at: 382 4991 382
Size: 4609 865
SfcI C'TryA_G
Cuts at: 33 420 976 988 1606 1657 2517 3202 4195
Size: 387 556 12 618 51 860 685 993
Cuts at: 4195 4386 33
Size: 191 1121
Fragments arranged by size:
1121 993 860 685 618 556 387 191
51 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 256 256
Size: 5474
SfoI GGC'GCC
Cuts at: 1472 2906 3154 1472
Size: 1434 248 3792
Fragments arranged by size:
3792 1434 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
SmaI CCC'GGG
Cuts at: 850 1048 1170 1195 2496 850
Size: 198 122 25 1301 3828
Fragments arranged by size:
3828 1301 198 122 25
SmlI C'TyrA_G
Cuts at: 715 964 1591 1954 2236 2310 2629 3544 4036
Size: 249 627 363 282 74 319 915 492
Cuts at: 4036 4298 715
Size: 262 1891
Fragments arranged by size:
1891 915 627 492 363 319 282 262
249 74
SnaBI TAC'GTA
Cuts at: 2466 2466
Size: 5474
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5474
SphI G_CATG'C
Cuts at: 2527 3027 2527
Size: 500 4974
StuI AGG'CCT
Cuts at: 622 622
Size: 5474
StyI C'CwwG_G
Cuts at: 125 1907 2111 2413 2608 2962 125
Size: 1782 204 302 195 354 2637
Fragments arranged by size:
2637 1782 354 302 204 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 769 3057 3190 3649 3858 130
Size: 639 2288 133 459 209 1746
Fragments arranged by size:
2288 1746 639 459 209 133
TatI w'GTAC_w
Cuts at: 173 380 401 4989 173
Size: 207 21 4588 658
Fragments arranged by size:
4588 658 207 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 823 855 1318 1350 1558 1590 4991 5023 823
Size: 32 463 32 208 32 3401 32 1274
Fragments arranged by size:
3401 1274 463 208 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3271 3271
Size: 5474
XbaI T'CTAG_A
Cuts at: 2505 2505
Size: 5474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2969 2969
Size: 5474
XhoI C'TCGA_G
Cuts at: 1591 1591
Size: 5474
XmaI C'CCGG_G
Cuts at: 848 1046 1168 1193 2494 848
Size: 198 122 25 1301 3828
Fragments arranged by size:
3828 1301 198 122 25
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BaeI BfrBI
BmgBI BsiWI BsrGI BssHII BstZ17I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PsrI SacII SanDI SexAI
SgrAI SrfI SspI SwaI XmnI ZraI