Restriction map for
pF1KSDA1899
(Circular) (Six-base) MAPSORT of: pF1KSDA1899.seq Check: 4089 from: 1 to: 6209
pF1KSDA1899 6209 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1439 1870 1439
Size: 431 5778
AccI GT'mk_AC
Cuts at: 683 3247 683
Size: 2564 3645
Acc65I G'GTAC_C
Cuts at: 3225 3225
Size: 6209
AclI AA'CG_TT
Cuts at: 6049 6049
Size: 6209
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 641 1208 1813 1852 2459 4034 4466 5213
Size: 389 567 605 39 607 1575 432 747
Cuts at: 5213 5481 252
Size: 268 980
Fragments arranged by size:
1575 980 747 607 605 567 432 389
268 39
AflII C'TTAA_G
Cuts at: 654 2781 654
Size: 2127 4082
AflIII A'CryG_T
Cuts at: 1661 2342 3561 4665 5642 1661
Size: 681 1219 1104 977 2228
Fragments arranged by size:
2228 1219 1104 977 681
AgeI A'CCGG_T
Cuts at: 2942 4597 2942
Size: 1655 4554
AleI CACnn'nnGTG
Cuts at: 1768 1768
Size: 6209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1837 2033 1837
Size: 196 6013
AlwNI CAG_nnn'CTG
Cuts at: 1830 3514 5081 1830
Size: 1684 1567 2958
Fragments arranged by size:
2958 1684 1567
ApaI G_GGCC'C
Cuts at: 1227 1748 1227
Size: 521 5688
ApaLI G'TGCA_C
Cuts at: 1567 4979 1567
Size: 3412 2797
ApoI r'AATT_y
Cuts at: 326 337 394 674 2298 2582 2909 3213 5562
Size: 11 57 280 1624 284 327 304 2349
Cuts at: 5562 5818 326
Size: 256 717
Fragments arranged by size:
2349 1624 717 327 304 284 280 256
57 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 6209
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6209
AvaI C'yCGr_G
Cuts at: 3229 3229
Size: 6209
BamHI G'GATC_C
Cuts at: 726 3139 3234 726
Size: 2413 95 3701
Fragments arranged by size:
3701 2413 95
BanI G'GyrC_C
Cuts at: 1332 1574 1588 3225 3639 3887 3922 1332
Size: 242 14 1637 414 248 35 3619
Fragments arranged by size:
3619 1637 414 248 242 35 14
BanII G_rGCy'C
Cuts at: 191 1227 1748 3223 4253 191
Size: 1036 521 1475 1030 2147
Fragments arranged by size:
2147 1475 1036 1030 521
BbeI G_GCGC'C
Cuts at: 1592 3643 3891 1592
Size: 2051 248 3910
Fragments arranged by size:
3910 2051 248
BbsI GAAGACnn'nnnn_
Cuts at: 1927 1927
Size: 6209
BbvCI CC'TCA_GC
Cuts at: 2527 2527
Size: 6209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1809 1843 2098 2132 2749 2783 1809
Size: 34 255 34 617 34 5235
Fragments arranged by size:
5235 617 255 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4107 4868 6209 4107
Size: 761 1341 4107
Fragments arranged by size:
4107 1341 761
BclI T'GATC_A
Cuts at: 642 3729 642
Size: 3087 3122
BfrBI ATG'CAT
Cuts at: 2799 2799
Size: 6209
BglII A'GATC_T
Cuts at: 3724 5761 3724
Size: 2037 4172
BlpI GC'TnA_GC
Cuts at: 3321 3321
Size: 6209
Bme1580I G_kGCm'C
Cuts at: 1227 1571 1577 1748 3046 3834 3927 4983 1227
Size: 344 6 171 1298 788 93 1056 2453
Fragments arranged by size:
2453 1298 1056 788 344 171 93 6
BmgBI CAC'GTC
Cuts at: 180 2345 2452 180
Size: 2165 107 3937
Fragments arranged by size:
3937 2165 107
BmrI ACTGGGnnnn_n'
Cuts at: 410 561 810 3421 3502 3837 5981 410
Size: 151 249 2611 81 335 2144 638
Fragments arranged by size:
2611 2144 638 335 249 151 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 4576 2809
Size: 1767 4442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1090 1400 1734 2878 196
Size: 894 310 334 1144 3527
Fragments arranged by size:
3527 1144 894 334 310
Bpu10I CC'TnA_GC
Cuts at: 2527 2527
Size: 6209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 3385 4264 4756 5054 1336
Size: 2049 879 492 298 2491
Fragments arranged by size:
2491 2049 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5913 5913
Size: 6209
BsaAI yAC'GTr
Cuts at: 3201 3562 3201
Size: 361 5848
BsaBI GATnn'nnATC
Cuts at: 6 2894 3723 5760 6
Size: 2888 829 2037 455
Fragments arranged by size:
2888 2037 829 455
BsaHI Gr'CG_yC
Cuts at: 1589 3640 3888 4590 6086 1589
Size: 2051 248 702 1496 1712
Fragments arranged by size:
2051 1712 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2942 3398 3919 4597 4871 5018 5429 2942
Size: 456 521 678 274 147 411 3722
Fragments arranged by size:
3722 678 521 456 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1176 1206 1176
Size: 30 6179
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2333 2333
Size: 6209
BseYI C'CCAG_C
Cuts at: 3634 4392 4969 5465 3634
Size: 758 577 496 4378
Fragments arranged by size:
4378 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 1345
Size: 6209
BsiEI CG_ry'CG
Cuts at: 104 3751 3797 5005 104
Size: 3647 46 1208 1308
Fragments arranged by size:
3647 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 191 1571 1858 3223 4001 4191 4983 191
Size: 1380 287 1365 778 190 792 1417
Fragments arranged by size:
1417 1380 1365 792 778 287 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1503 1503
Size: 6209
Bsp1286I G_dGCh'C
Cuts at: 191 1227 1571 1577 1748 1858 3046 3223 3834
Size: 1036 344 6 171 110 1188 177 611
Cuts at: 3834 3927 4001 4191 4253 4983 191
Size: 93 74 190 62 730 1417
Fragments arranged by size:
1417 1188 1036 730 611 344 190 177
171 110 93 74 62 6
BspEI T'CCGG_A
Cuts at: 5429 5429
Size: 6209
BspHI T'CATG_A
Cuts at: 2049 2828 2987 5385 2049
Size: 779 159 2398 2873
Fragments arranged by size:
2873 2398 779 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1439 1550 1847 1870 3259 3473 3775 4156 1439
Size: 111 297 23 1389 214 302 381 3492
Fragments arranged by size:
3492 1389 381 302 297 214 111 23
BsrBI CCG'CTC
Cuts at: 50 4501 4555 6039 6204 50
Size: 4451 54 1484 165 55
Fragments arranged by size:
4451 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 4121 2015
Size: 2106 4103
BsrFI r'CCGG_y
Cuts at: 205 2942 4207 4388 4597 205
Size: 2737 1265 181 209 1817
Fragments arranged by size:
2737 1817 1265 209 181
BsrGI T'GTAC_A
Cuts at: 875 1229 1445 1923 875
Size: 354 216 478 5161
Fragments arranged by size:
5161 478 354 216
BssSI C'ACGA_G
Cuts at: 2107 4480 4838 2107
Size: 2373 358 3478
Fragments arranged by size:
3478 2373 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1862 3586 1862
Size: 1724 4485
BstBI TT'CG_AA
Cuts at: 110 4570 110
Size: 4460 1749
BstYI r'GATC_y
Cuts at: 1 726 2188 3139 3234 3701 3724 4059 4305
Size: 725 1462 951 95 467 23 335 246
Cuts at: 4305 5317 5403 5415 5761 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1462 1012 951 725 467 449 346 335
246 95 86 23 12
BtgI C'CryG_G
Cuts at: 125 145 1766 2542 2549 125
Size: 20 1621 776 7 3785
Fragments arranged by size:
3785 1621 776 20 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2752 4159 4340 4503 2752
Size: 1407 181 163 4458
Fragments arranged by size:
4458 1407 181 163
BtsI GCAGTG_nn'
Cuts at: 888 1740 1849 2056 3452 888
Size: 852 109 207 1396 3645
Fragments arranged by size:
3645 1396 852 207 109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1301 1336 2602 2637 1301
Size: 35 1266 35 4873
Fragments arranged by size:
4873 1266 35 35
DraI TTT'AAA
Cuts at: 3087 3119 3208 3087
Size: 32 89 6088
Fragments arranged by size:
6088 89 32
DraIII CAC_nnn'GTG
Cuts at: 1368 1368
Size: 6209
DrdI GACnn_nn'nnGTC
Cuts at: 3915 4773 3915
Size: 858 5351
EaeI y'GGCC_r
Cuts at: 1086 3794 3968 4359 4386 5731 1086
Size: 2708 174 391 27 1345 1564
Fragments arranged by size:
2708 1564 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3794 3794
Size: 6209
EarI CTCTTCn'nnn_
Cuts at: 373 626 4232 4442 373
Size: 253 3606 210 2140
Fragments arranged by size:
3606 2140 253 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4417 4727 4873 6019 4417
Size: 310 146 1146 4607
Fragments arranged by size:
4607 1146 310 146
EcoICRI GAG'CTC
Cuts at: 189 3221 189
Size: 3032 3177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 252 641 1090 1208 1400 1734 1813 1852
Size: 56 389 449 118 192 334 79 39
Cuts at: 1852 2459 2878 4034 4466 5213 5481 196
Size: 607 419 1156 432 747 268 924
Fragments arranged by size:
1156 924 747 607 449 432 419 389
334 268 192 118 79 56 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1447 1752 1447
Size: 305 5904
EcoO109I rG'GnC_Cy
Cuts at: 809 1224 1744 1745 2538 3348 809
Size: 415 520 1 793 810 3670
Fragments arranged by size:
3670 810 793 520 415 1
EcoRI G'AATT_C
Cuts at: 674 3213 674
Size: 2539 3670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1276 2617 2716 2985 4228 1276
Size: 1341 99 269 1243 3257
Fragments arranged by size:
3257 1341 1243 269 99
FspI TGC'GCA
Cuts at: 3990 3990
Size: 6209
HaeII r_GCGC'y
Cuts at: 1592 3643 3891 4913 5892 1592
Size: 2051 248 1022 979 1909
Fragments arranged by size:
2051 1909 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2131 2163 2180 2212 2948 2980 4051 4081 4083
Size: 32 17 32 736 32 1071 30 2
Cuts at: 4083 4113 2131
Size: 30 4227
Fragments arranged by size:
4227 1071 736 32 32 32 30 30
17 2
HincII GTy'rAC
Cuts at: 294 1184 2478 2930 3248 294
Size: 890 1294 452 318 3255
Fragments arranged by size:
3255 1294 890 452 318
KasI G'GCGC_C
Cuts at: 1588 3639 3887 1588
Size: 2051 248 3910
Fragments arranged by size:
3910 2051 248
KpnI G_GTAC'C
Cuts at: 3229 3229
Size: 6209
MfeI C'AATT_G
Cuts at: 2899 2899
Size: 6209
MluI A'CGCG_T
Cuts at: 5642 5642
Size: 6209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 287 355 721 1455 2430 4880 5064 137
Size: 150 68 366 734 975 2450 184 1282
Fragments arranged by size:
2450 1282 975 734 366 184 150 68
MscI TGG'CCA
Cuts at: 1088 3970 1088
Size: 2882 3327
MslI CAynn'nnrTG
Cuts at: 1768 2992 4198 4325 1768
Size: 1224 1206 127 3652
Fragments arranged by size:
3652 1224 1206 127
MspA1I CmG'CkG
Cuts at: 1827 3320 3634 3994 5007 5252 1827
Size: 1493 314 360 1013 245 2784
Fragments arranged by size:
2784 1493 1013 360 314 245
NaeI GCC'GGC
Cuts at: 207 4390 207
Size: 4183 2026
NarI GG'CG_CC
Cuts at: 1589 3640 3888 1589
Size: 2051 248 3910
Fragments arranged by size:
3910 2051 248
NcoI C'CATG_G
Cuts at: 125 1766 2542 125
Size: 1641 776 3792
Fragments arranged by size:
3792 1641 776
NdeI CA'TA_TG
Cuts at: 1083 3098 1083
Size: 2015 4194
NgoMIV G'CCGG_C
Cuts at: 205 4388 205
Size: 4183 2026
NsiI A_TGCA'T
Cuts at: 2801 2801
Size: 6209
NspI r_CATG'y
Cuts at: 1152 2165 2775 3262 3762 4669 1152
Size: 1013 610 487 500 907 2692
Fragments arranged by size:
2692 1013 907 610 500 487
PciI A'CATG_T
Cuts at: 4665 4665
Size: 6209
PfoI T'CCnGG_A
Cuts at: 212 1106 212
Size: 894 5315
PmeI GTTT'AAAC
Cuts at: 3208 3208
Size: 6209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5348 5380 5348
Size: 32 6177
PpuMI rG'GwC_Cy
Cuts at: 2538 2538
Size: 6209
PshAI GACnn'nnGTC
Cuts at: 590 2945 590
Size: 2355 3854
PsiI TTA'TAA
Cuts at: 2563 2588 2563
Size: 25 6184
PspOMI G'GGCC_C
Cuts at: 1223 1744 1223
Size: 521 5688
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1376 1408 1376
Size: 32 6177
PstI C_TGCA'G
Cuts at: 1547 1844 2676 3256 3941 1547
Size: 297 832 580 685 3815
Fragments arranged by size:
3815 832 685 580 297
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6209
PvuII CAG'CTG
Cuts at: 1827 3634 3994 1827
Size: 1807 360 4042
Fragments arranged by size:
4042 1807 360
RsrII CG'GwC_CG
Cuts at: 4404 4404
Size: 6209
SacI G_AGCT'C
Cuts at: 191 3223 191
Size: 3032 3177
SalI G'TCGA_C
Cuts at: 3246 3246
Size: 6209
SapI GCTCTTCn'nnn_
Cuts at: 4232 4442 4232
Size: 210 5999
SbfI CC_TGCA'GG
Cuts at: 3256 3256
Size: 6209
ScaI AGT'ACT
Cuts at: 796 1377 2670 5726 796
Size: 581 1293 3056 1279
Fragments arranged by size:
3056 1293 1279 581
SfcI C'TryA_G
Cuts at: 33 1543 1840 2672 2916 3062 3132 3252 3937
Size: 1510 297 832 244 146 70 120 685
Cuts at: 3937 4930 5121 33
Size: 993 191 1121
Fragments arranged by size:
1510 1121 993 832 685 297 244 191
146 120 70
SfoI GGC'GCC
Cuts at: 1590 3641 3889 1590
Size: 2051 248 3910
Fragments arranged by size:
3910 2051 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6209
SmaI CCC'GGG
Cuts at: 3231 3231
Size: 6209
SmlI C'TyrA_G
Cuts at: 654 1351 2781 3364 4279 4771 5033 654
Size: 697 1430 583 915 492 262 1830
Fragments arranged by size:
1830 1430 915 697 583 492 262
SnaBI TAC'GTA
Cuts at: 3201 3201
Size: 6209
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6209
SphI G_CATG'C
Cuts at: 1152 3262 3762 1152
Size: 2110 500 3599
Fragments arranged by size:
3599 2110 500
SspI AAT'ATT
Cuts at: 1023 1237 1023
Size: 214 5995
StuI AGG'CCT
Cuts at: 2699 3038 2699
Size: 339 5870
StyI C'CwwG_G
Cuts at: 125 1766 1988 2542 2885 3343 3697 125
Size: 1641 222 554 343 458 354 2637
Fragments arranged by size:
2637 1641 554 458 354 343 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 776 1509 3792 3925 4384 4593 776
Size: 733 2283 133 459 209 2392
Fragments arranged by size:
2392 2283 733 459 209 133
TatI w'GTAC_w
Cuts at: 794 875 1229 1375 1445 1923 2668 2848 5724
Size: 81 354 146 70 478 745 180 2876
Cuts at: 5724 794
Size: 1279
Fragments arranged by size:
2876 1279 745 478 354 180 146 81
70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5726 5758 5726
Size: 32 6177
Tth111I GACn'n_nGTC
Cuts at: 4006 4006
Size: 6209
XbaI T'CTAG_A
Cuts at: 2223 3240 2223
Size: 1017 5192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3704 3704
Size: 6209
XmaI C'CCGG_G
Cuts at: 3229 3229
Size: 6209
XmnI GAAnn'nnTTC
Cuts at: 2302 2302
Size: 6209
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AvrII BaeI BglI
BmtI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI EcoRV FseI FspAI HindIII HpaI NheI NotI
NruI PacI PasI PflMI PmlI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XhoI ZraI