Restriction map for
pF1KSDA1892
(Circular) (Six-base) MAPSORT of: pF1KSDA1892.seq Check: 5846 from: 1 to: 4496
pF1KSDA1892 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 266 1534 266
Size: 1268 3228
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1433 2321 2753 3500 3768 382
Size: 1051 888 432 747 268 1110
Fragments arranged by size:
1110 1051 888 747 432 268
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 350 350
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 724 1801 3368 724
Size: 1077 1567 1852
Fragments arranged by size:
1852 1567 1077
ApaI G_GGCC'C
Cuts at: 199 199
Size: 4496
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 1500 3849 4105 1500
Size: 2349 256 1891
Fragments arranged by size:
2349 1891 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 317 1516 317
Size: 1199 3297
BamHI G'GATC_C
Cuts at: 645 1092 1521 645
Size: 447 429 3620
Fragments arranged by size:
3620 447 429
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 199 897 1069 1207 1233 1457 1478 1510 2540
Size: 698 172 138 26 224 21 32 1030
Cuts at: 2540 199
Size: 2155
Fragments arranged by size:
2155 1030 698 224 172 138 32 26
21
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 869 869
Size: 4496
BciVI GTATCCnnnnn_n'
Cuts at: 562 2394 3155 4496 562
Size: 1832 761 1341 562
Fragments arranged by size:
1832 1341 761 562
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 199 2121 2214 3270 199
Size: 1922 93 1056 1425
Fragments arranged by size:
1922 1425 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 347 934 1708 1789 2124 4268 347
Size: 587 774 81 335 2144 575
Fragments arranged by size:
2144 774 587 575 335 81
BmtI G_CTAG'C
Cuts at: 592 592
Size: 4496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 206 1272 1459 2863 206
Size: 1066 187 1404 1839
Fragments arranged by size:
1839 1404 1066 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 617 1365 1500 545
Size: 72 748 135 3541
Fragments arranged by size:
3541 748 135 72
Bpu10I CC'TnA_GC
Cuts at: 191 191
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 733 1488 1849 733
Size: 755 361 3380
Fragments arranged by size:
3380 755 361
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1154 1685 2206 2884 3158 3305 3716 1154
Size: 531 521 678 274 147 411 1934
Fragments arranged by size:
1934 678 531 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 358
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 614 979 614
Size: 365 4131
BseYI C'CCAG_C
Cuts at: 178 209 536 1471 1921 2679 3256 3752 178
Size: 31 327 935 450 758 577 496 922
Fragments arranged by size:
935 922 758 577 496 450 327 31
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1178 1233 1510 2288 2478 3270 1178
Size: 55 277 778 190 792 2404
Fragments arranged by size:
2404 792 778 277 190 55
Bsp1286I G_dGCh'C
Cuts at: 199 897 1069 1178 1207 1233 1457 1478 1510
Size: 698 172 109 29 26 224 21 32
Cuts at: 1510 2121 2214 2288 2478 2540 3270 199
Size: 611 93 74 190 62 730 1425
Fragments arranged by size:
1425 730 698 611 224 190 172 109
93 74 62 32 29 26 21
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 1332 3672 1332
Size: 2340 2156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 2494 2675 2884 2494
Size: 181 209 4106
Fragments arranged by size:
4106 209 181
BssSI C'ACGA_G
Cuts at: 2767 3125 2767
Size: 358 4138
BstAPI GCAn_nnn'nTGC
Cuts at: 449 1873 449
Size: 1424 3072
BstBI TT'CG_AA
Cuts at: 110 2857 110
Size: 2747 1749
BstYI r'GATC_y
Cuts at: 1 256 497 645 683 1092 1521 1988 2011
Size: 255 241 148 38 409 429 467 23
Cuts at: 2011 2346 2592 3604 3690 3702 4048 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 429 409 346 335 255
246 241 148 86 38 23 12
BstZ17I GTA'TAC
Cuts at: 267 267
Size: 4496
BtgI C'CryG_G
Cuts at: 125 1096 1458 125
Size: 971 362 3163
Fragments arranged by size:
3163 971 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 678 2446 2627 2790 678
Size: 1768 181 163 2384
Fragments arranged by size:
2384 1768 181 163
BtsI GCAGTG_nn'
Cuts at: 583 725 938 1067 1152 1395 1739 583
Size: 142 213 129 85 243 344 3340
Fragments arranged by size:
3340 344 243 213 142 129 85
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DraIII CAC_nnn'GTG
Cuts at: 801 801
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 2081 2255 2646 2673 4018 2081
Size: 174 391 27 1345 2559
Fragments arranged by size:
2559 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 247 1241 2519 2729 247
Size: 994 1278 210 2014
Fragments arranged by size:
2014 1278 994 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1231 1508 1231
Size: 277 4219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 545 617 1365 1433 1500 2321 2753 3500
Size: 163 72 748 68 67 821 432 747
Cuts at: 3500 3768 382
Size: 268 1110
Fragments arranged by size:
1110 821 748 747 432 268 163 72
68 67
EcoO109I rG'GnC_Cy
Cuts at: 195 386 810 1438 1635 195
Size: 191 424 628 197 3056
Fragments arranged by size:
3056 628 424 197 191
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 276 2515 276
Size: 2239 2257
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 163 1930 2178 3200 4179 163
Size: 1767 248 1022 979 480
Fragments arranged by size:
1767 1022 979 480 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2338 2368 2370 2400 2338
Size: 30 2 30 4434
Fragments arranged by size:
4434 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 332 411 1186 2485 2612 332
Size: 79 775 1299 127 2216
Fragments arranged by size:
2216 1299 775 127 79
MspA1I CmG'CkG
Cuts at: 1607 1921 2281 3294 3539 1607
Size: 314 360 1013 245 2564
Fragments arranged by size:
2564 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 125 1458 125
Size: 1333 3163
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NheI G'CTAG_C
Cuts at: 588 588
Size: 4496
NspI r_CATG'y
Cuts at: 1549 2049 2956 1549
Size: 500 907 3089
Fragments arranged by size:
3089 907 500
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 698 698
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PmlI CAC'GTG
Cuts at: 733 733
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 386 810 1438 386
Size: 424 628 3444
Fragments arranged by size:
3444 628 424
PspOMI G'GGCC_C
Cuts at: 195 195
Size: 4496
PstI C_TGCA'G
Cuts at: 1543 2228 1543
Size: 685 3811
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1233 1510 1233
Size: 277 4219
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SanDI GG'GwC_CC
Cuts at: 386 810 386
Size: 424 4072
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 295 1539 2224 3217 3408 33
Size: 262 1244 685 993 191 1121
Fragments arranged by size:
1244 1121 993 685 262 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 317 1651 2566 3058 3320 317
Size: 1334 915 492 262 1493
Fragments arranged by size:
1493 1334 915 492 262
SnaBI TAC'GTA
Cuts at: 1488 1488
Size: 4496
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StuI AGG'CCT
Cuts at: 841 841
Size: 4496
StyI C'CwwG_G
Cuts at: 125 381 1458 1630 1984 125
Size: 256 1077 172 354 2637
Fragments arranged by size:
2637 1077 354 256 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1408 2079 2212 2671 2880 1408
Size: 671 133 459 209 3024
Fragments arranged by size:
3024 671 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 637 669 4013 4045 637
Size: 32 3344 32 1088
Fragments arranged by size:
3344 1088 32 32
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 577 1527 577
Size: 950 3546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 544 1991 544
Size: 1447 3049
XhoI C'TCGA_G
Cuts at: 317 317
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SrfI SspI SwaI XmnI ZraI