Restriction map for
pF1KSDA1860
(Circular) (Six-base) MAPSORT of: pF1KSDA1860.seq Check: 4714 from: 1 to: 5203
pF1KSDA1860 5203 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 2239 2239
Size: 5203
Acc65I G'GTAC_C
Cuts at: 2217 2217
Size: 5203
AclI AA'CG_TT
Cuts at: 5043 5043
Size: 5203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 714 1486 3026 3458 4207 4475 691
Size: 23 772 1540 432 749 268 1419
Fragments arranged by size:
1540 1419 772 749 432 268 23
AflIII A'CryG_T
Cuts at: 764 959 2553 3659 4636 764
Size: 195 1594 1106 977 1331
Fragments arranged by size:
1594 1331 1106 977 195
AgeI A'CCGG_T
Cuts at: 3591 3591
Size: 5203
AhdI GACnn_n'nnGTC
Cuts at: 2118 2118
Size: 5203
AlwNI CAG_nnn'CTG
Cuts at: 432 2506 4075 432
Size: 2074 1569 1560
Fragments arranged by size:
2074 1569 1560
ApaI G_GGCC'C
Cuts at: 1898 1898
Size: 5203
ApaLI G'TGCA_C
Cuts at: 631 3973 631
Size: 3342 1861
ApoI r'AATT_y
Cuts at: 2205 4556 4812 2205
Size: 2351 256 2596
Fragments arranged by size:
2596 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5203
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5203
AvaI C'yCGr_G
Cuts at: 150 274 599 1027 1099 1677 2221 150
Size: 124 325 428 72 578 544 3132
Fragments arranged by size:
3132 578 544 428 325 124 72
BamHI G'GATC_C
Cuts at: 2071 2226 2071
Size: 155 5048
BanI G'GyrC_C
Cuts at: 181 241 1258 1290 1330 1729 1740 1773 2053
Size: 60 1017 32 40 399 11 33 280
Cuts at: 2053 2217 2631 2879 2914 181
Size: 164 414 248 35 2470
Fragments arranged by size:
2470 1017 414 399 280 248 164 60
40 35 33 32 11
BanII G_rGCy'C
Cuts at: 781 1100 1898 2215 3245 781
Size: 319 798 317 1030 2739
Fragments arranged by size:
2739 1030 798 319 317
BbeI G_GCGC'C
Cuts at: 1262 1294 2057 2635 2883 1262
Size: 32 763 578 248 3582
Fragments arranged by size:
3582 763 578 248 32
BbsI GAAGACnn'nnnn_
Cuts at: 122 517 1240 122
Size: 395 723 4085
Fragments arranged by size:
4085 723 395
BbvCI CC'TCA_GC
Cuts at: 310 310
Size: 5203
BciVI GTATCCnnnnn_n'
Cuts at: 3099 3862 5203 3099
Size: 763 1341 3099
Fragments arranged by size:
3099 1341 763
BclI T'GATC_A
Cuts at: 2721 2721
Size: 5203
BglI GCCn_nnn'nGGC
Cuts at: 1924 1944 1924
Size: 20 5183
BglII A'GATC_T
Cuts at: 2716 4755 2716
Size: 2039 3164
BlpI GC'TnA_GC
Cuts at: 1685 2313 1685
Size: 628 4575
Bme1580I G_kGCm'C
Cuts at: 246 635 902 1745 1898 2826 2919 3977 246
Size: 389 267 843 153 928 93 1058 1472
Fragments arranged by size:
1472 1058 928 843 389 267 153 93
BmrI ACTGGGnnnn_n'
Cuts at: 247 2413 2494 2829 4975 247
Size: 2166 81 335 2146 475
Fragments arranged by size:
2166 2146 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3568 3568
Size: 5203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 864 1524 1743 2204 566
Size: 298 660 219 461 3565
Fragments arranged by size:
3565 660 461 298 219
Bpu10I CC'TnA_GC
Cuts at: 310 1725 310
Size: 1415 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 2377 3256 3750 4048 892
Size: 1485 879 494 298 2047
Fragments arranged by size:
2047 1485 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4907 4907
Size: 5203
BsaAI yAC'GTr
Cuts at: 291 2193 2554 291
Size: 1902 361 2940
Fragments arranged by size:
2940 1902 361
BsaBI GATnn'nnATC
Cuts at: 6 1057 2715 4754 6
Size: 1051 1658 2039 455
Fragments arranged by size:
2039 1658 1051 455
BsaHI Gr'CG_yC
Cuts at: 1259 1291 2054 2632 2880 3584 5080 1259
Size: 32 763 578 248 704 1496 1382
Fragments arranged by size:
1496 1382 763 704 578 248 32
BsaWI w'CCGG_w
Cuts at: 2390 2911 3591 3865 4012 4423 2390
Size: 521 680 274 147 411 3170
Fragments arranged by size:
3170 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1506 1536 2177 2207 1506
Size: 30 641 30 4502
Fragments arranged by size:
4502 641 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 292 1090 1117 1255 1486 192
Size: 100 798 27 138 231 3909
Fragments arranged by size:
3909 798 231 138 100 27
BseYI C'CCAG_C
Cuts at: 404 415 747 1008 1224 1294 2626 3384 3963
Size: 11 332 261 216 70 1332 758 579
Cuts at: 3963 4459 404
Size: 496 1148
Fragments arranged by size:
1332 1148 758 579 496 332 261 216
70 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1400 1408 1906 1913 1400
Size: 8 498 7 4690
Fragments arranged by size:
4690 498 8 7
BsiEI CG_ry'CG
Cuts at: 104 162 2743 2789 3999 104
Size: 58 2581 46 1210 1308
Fragments arranged by size:
2581 1308 1210 58 46
BsiHKAI G_wGCw'C
Cuts at: 635 1450 1615 2215 2993 3183 3977 635
Size: 815 165 600 778 190 794 1861
Fragments arranged by size:
1861 815 794 778 600 190 165
Bsp1286I G_dGCh'C
Cuts at: 246 635 781 902 1100 1450 1615 1745 1898
Size: 389 146 121 198 350 165 130 153
Cuts at: 1898 2215 2826 2919 2993 3183 3245 3977 246
Size: 317 611 93 74 190 62 732 1472
Fragments arranged by size:
1472 732 611 389 350 317 198 190
165 153 146 130 121 93 74 62
BspEI T'CCGG_A
Cuts at: 4423 4423
Size: 5203
BspHI T'CATG_A
Cuts at: 452 1037 4379 452
Size: 585 3342 1276
Fragments arranged by size:
3342 1276 585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2251 2465 2767 3148 2251
Size: 214 302 381 4306
Fragments arranged by size:
4306 381 302 214
BsrBI CCG'CTC
Cuts at: 50 200 233 1476 1593 1791 3493 3547 5033
Size: 150 33 1243 117 198 1702 54 1486
Cuts at: 5033 5198 50
Size: 165 55
Fragments arranged by size:
1702 1486 1243 198 165 150 117 55
54 33
BsrDI GCAATG_nn'
Cuts at: 3113 3113
Size: 5203
BsrFI r'CCGG_y
Cuts at: 1853 3199 3380 3591 1853
Size: 1346 181 211 3465
Fragments arranged by size:
3465 1346 211 181
BsrGI T'GTAC_A
Cuts at: 657 863 657
Size: 206 4997
BssSI C'ACGA_G
Cuts at: 565 1608 3472 3832 565
Size: 1043 1864 360 1936
Fragments arranged by size:
1936 1864 1043 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2578 2578
Size: 5203
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5203
BstEII G'GTnAC_C
Cuts at: 2004 2004
Size: 5203
BstXI CCAn_nnnn'nTGG
Cuts at: 1548 1548
Size: 5203
BstYI r'GATC_y
Cuts at: 1 1583 2071 2226 2693 2716 3051 3297 4311
Size: 1582 488 155 467 23 335 246 1014
Cuts at: 4311 4397 4409 4755 1
Size: 86 12 346 449
Fragments arranged by size:
1582 1014 488 467 449 346 335 246
155 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2116 2116
Size: 5203
BtgI C'CryG_G
Cuts at: 1508 1547 1735 1833 1508
Size: 39 188 98 4878
Fragments arranged by size:
4878 188 98 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 246 3151 3332 3495 246
Size: 2905 181 163 1954
Fragments arranged by size:
2905 1954 181 163
BtsI GCAGTG_nn'
Cuts at: 198 2444 198
Size: 2246 2957
DraI TTT'AAA
Cuts at: 2200 2200
Size: 5203
DraIII CAC_nnn'GTG
Cuts at: 241 241
Size: 5203
DrdI GACnn_nn'nnGTC
Cuts at: 2907 3767 2907
Size: 860 4343
EaeI y'GGCC_r
Cuts at: 195 576 1839 2786 2960 3351 3378 4725 195
Size: 381 1263 947 174 391 27 1347 673
Fragments arranged by size:
1347 1263 947 673 391 381 174 27
EagI C'GGCC_G
Cuts at: 2786 2786
Size: 5203
EarI CTCTTCn'nnn_
Cuts at: 1368 1975 3224 3434 1368
Size: 607 1249 210 3137
Fragments arranged by size:
3137 1249 607 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1474 1611 3409 3721 3867 5013 1474
Size: 137 1798 312 146 1146 1664
Fragments arranged by size:
1798 1664 1146 312 146 137
EcoICRI GAG'CTC
Cuts at: 2213 2213
Size: 5203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 691 714 864 1486 1524 1743 2204 3026
Size: 125 23 150 622 38 219 461 822
Cuts at: 3026 3458 4207 4475 566
Size: 432 749 268 1294
Fragments arranged by size:
1294 822 749 622 461 432 268 219
150 125 38 23
EcoO109I rG'GnC_Cy
Cuts at: 459 666 948 1895 2112 2340 459
Size: 207 282 947 217 228 3322
Fragments arranged by size:
3322 947 282 228 217 207
EcoRI G'AATT_C
Cuts at: 2205 2205
Size: 5203
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 348 3220 348
Size: 2872 2331
FspI TGC'GCA
Cuts at: 2982 2982
Size: 5203
HaeII r_GCGC'y
Cuts at: 1262 1294 1562 1692 2057 2635 2883 3907 4886
Size: 32 268 130 365 578 248 1024 979
Cuts at: 4886 1262
Size: 1579
Fragments arranged by size:
1579 1024 979 578 365 268 248 130
32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 1506 1538 1678 1710 2063 2095 2104
Size: 32 1323 32 140 32 353 32 9
Cuts at: 2104 2136 3043 3073 3075 3105 151
Size: 32 907 30 2 30 2249
Fragments arranged by size:
2249 1323 907 353 140 32 32 32
32 32 30 30 9 2
HincII GTy'rAC
Cuts at: 965 2240 965
Size: 1275 3928
KasI G'GCGC_C
Cuts at: 1258 1290 2053 2631 2879 1258
Size: 32 763 578 248 3582
Fragments arranged by size:
3582 763 578 248 32
KpnI G_GTAC'C
Cuts at: 2221 2221
Size: 5203
MluI A'CGCG_T
Cuts at: 4636 4636
Size: 5203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 636 3874 4058 636
Size: 3238 184 1781
Fragments arranged by size:
3238 1781 184
MscI TGG'CCA
Cuts at: 1841 2962 1841
Size: 1121 4082
MslI CAynn'nnrTG
Cuts at: 3190 3317 3190
Size: 127 5076
MspA1I CmG'CkG
Cuts at: 408 878 1353 1510 2042 2312 2626 2986 4001
Size: 470 475 157 532 270 314 360 1015
Cuts at: 4001 4246 408
Size: 245 1365
Fragments arranged by size:
1365 1015 532 475 470 360 314 270
245 157
NaeI GCC'GGC
Cuts at: 3382 3382
Size: 5203
NarI GG'CG_CC
Cuts at: 1259 1291 2054 2632 2880 1259
Size: 32 763 578 248 3582
Fragments arranged by size:
3582 763 578 248 32
NcoI C'CATG_G
Cuts at: 1547 1833 1547
Size: 286 4917
NdeI CA'TA_TG
Cuts at: 786 786
Size: 5203
NgoMIV G'CCGG_C
Cuts at: 3380 3380
Size: 5203
NruI TCG'CGA
Cuts at: 3564 3564
Size: 5203
NspI r_CATG'y
Cuts at: 963 2254 2754 3663 963
Size: 1291 500 909 2503
Fragments arranged by size:
2503 1291 909 500
PasI CC'CwG_GG
Cuts at: 1264 2067 1264
Size: 803 4400
PciI A'CATG_T
Cuts at: 959 3659 959
Size: 2700 2503
PfoI T'CCnGG_A
Cuts at: 1847 1847
Size: 5203
PmeI GTTT'AAAC
Cuts at: 2200 2200
Size: 5203
PmlI CAC'GTG
Cuts at: 291 291
Size: 5203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4342 4374 4342
Size: 32 5171
PpuMI rG'GwC_Cy
Cuts at: 666 948 2112 666
Size: 282 1164 3757
Fragments arranged by size:
3757 1164 282
PspOMI G'GGCC_C
Cuts at: 1894 1894
Size: 5203
PspXI vC'TCGA_Gb
Cuts at: 599 599
Size: 5203
PstI C_TGCA'G
Cuts at: 428 1504 2248 2933 428
Size: 1076 744 685 2698
Fragments arranged by size:
2698 1076 744 685
PvuI CG_AT'CG
Cuts at: 104 162 104
Size: 58 5145
PvuII CAG'CTG
Cuts at: 408 2626 2986 408
Size: 2218 360 2625
Fragments arranged by size:
2625 2218 360
RsrII CG'GwC_CG
Cuts at: 3396 3396
Size: 5203
SacI G_AGCT'C
Cuts at: 2215 2215
Size: 5203
SacII CC_GC'GG
Cuts at: 1511 1511
Size: 5203
SalI G'TCGA_C
Cuts at: 2238 2238
Size: 5203
SanDI GG'GwC_CC
Cuts at: 948 948
Size: 5203
SapI GCTCTTCn'nnn_
Cuts at: 3224 3434 3224
Size: 210 4993
SbfI CC_TGCA'GG
Cuts at: 2248 2248
Size: 5203
ScaI AGT'ACT
Cuts at: 929 4720 929
Size: 3791 1412
SexAI A'CCwGG_T
Cuts at: 521 521
Size: 5203
SfcI C'TryA_G
Cuts at: 33 424 1500 2244 2929 3924 4115 33
Size: 391 1076 744 685 995 191 1121
Fragments arranged by size:
1121 1076 995 744 685 391 191
SfoI GGC'GCC
Cuts at: 1260 1292 2055 2633 2881 1260
Size: 32 763 578 248 3582
Fragments arranged by size:
3582 763 578 248 32
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5203
SmaI CCC'GGG
Cuts at: 152 1679 2223 152
Size: 1527 544 3132
Fragments arranged by size:
3132 1527 544
SmlI C'TyrA_G
Cuts at: 599 907 1027 2356 3271 3765 4027 599
Size: 308 120 1329 915 494 262 1775
Fragments arranged by size:
1775 1329 915 494 308 262 120
SnaBI TAC'GTA
Cuts at: 2193 2193
Size: 5203
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5203
SphI G_CATG'C
Cuts at: 2254 2754 2254
Size: 500 4703
SspI AAT'ATT
Cuts at: 654 654
Size: 5203
StyI C'CwwG_G
Cuts at: 185 1547 1833 2139 2335 2689 185
Size: 1362 286 306 196 354 2699
Fragments arranged by size:
2699 1362 354 306 286 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 2784 2917 3376 3587 229
Size: 2555 133 459 211 1845
Fragments arranged by size:
2555 1845 459 211 133
TatI w'GTAC_w
Cuts at: 657 863 927 1021 1193 4718 657
Size: 206 64 94 172 3525 1142
Fragments arranged by size:
3525 1142 206 172 94 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4720 4752 4720
Size: 32 5171
Tth111I GACn'n_nGTC
Cuts at: 2998 2998
Size: 5203
XbaI T'CTAG_A
Cuts at: 2232 2232
Size: 5203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 345 1767 2696 234
Size: 111 1422 929 2741
Fragments arranged by size:
2741 1422 929 111
XhoI C'TCGA_G
Cuts at: 599 1027 599
Size: 428 4775
XmaI C'CCGG_G
Cuts at: 150 1677 2221 150
Size: 1527 544 3132
Fragments arranged by size:
3132 1527 544
XmnI GAAnn'nnTTC
Cuts at: 433 1317 433
Size: 884 4319
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BssHII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NsiI PacI
PflMI PshAI PsiI PsrI SfiI SgrAI SrfI StuI
SwaI ZraI