Restriction map for
pF1KSDA1823
(Circular) (Six-base) MAPSORT of: pF1KSDA1823.seq Check: 8508 from: 1 to: 4232
pF1KSDA1823 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 2057 2489 3236 3504 916
Size: 1141 432 747 268 1644
Fragments arranged by size:
1644 1141 747 432 268
AflIII A'CryG_T
Cuts at: 418 1584 2688 3665 418
Size: 1166 1104 977 985
Fragments arranged by size:
1166 1104 985 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 491 901 1085 1236 3585 3841 491
Size: 410 184 151 2349 256 882
Fragments arranged by size:
2349 882 410 256 184 151
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 1252 1252
Size: 4232
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 1248 1662 1910 1945 1248
Size: 414 248 35 3535
Fragments arranged by size:
3535 414 248 35
BanII G_rGCy'C
Cuts at: 584 1246 2276 584
Size: 662 1030 2540
Fragments arranged by size:
2540 1030 662
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1182 1752 1182
Size: 570 3662
BglI GCCn_nnn'nGGC
Cuts at: 829 829
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 1857 1950 3006 1857
Size: 93 1056 3083
Fragments arranged by size:
3083 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1444 1525 1860 4004 1444
Size: 81 335 2144 1672
Fragments arranged by size:
2144 1672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 598 410
Size: 188 4044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 2779 3077 1408
Size: 879 492 298 2563
Fragments arranged by size:
2563 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 725 3936 725
Size: 3211 1021
BsaAI yAC'GTr
Cuts at: 1224 1585 1224
Size: 361 3871
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1657 2415 2992 3488 1657
Size: 758 577 496 2401
Fragments arranged by size:
2401 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 512
Size: 4232
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1246 2024 2214 3006 1246
Size: 778 190 792 2472
Fragments arranged by size:
2472 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 584 1246 1857 1950 2024 2214 2276 3006 584
Size: 662 611 93 74 190 62 730 1810
Fragments arranged by size:
1810 730 662 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1282 1496 1798 2179 1282
Size: 214 302 381 3335
Fragments arranged by size:
3335 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 448 2144 448
Size: 1696 2536
BsrFI r'CCGG_y
Cuts at: 2230 2411 2620 2230
Size: 181 209 3842
Fragments arranged by size:
3842 209 181
BsrGI T'GTAC_A
Cuts at: 964 964
Size: 4232
BssSI C'ACGA_G
Cuts at: 797 1079 2503 2861 797
Size: 282 1424 358 2168
Fragments arranged by size:
2168 1424 358 282
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 110 2593 110
Size: 2483 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 388 388
Size: 4232
BstYI r'GATC_y
Cuts at: 1 1257 1724 1747 2082 2328 3340 3426 3438
Size: 1256 467 23 335 246 1012 86 12
Cuts at: 3438 3784 1
Size: 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 678 678
Size: 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 1475 1475
Size: 4232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 189 224 189
Size: 35 4197
DraI TTT'AAA
Cuts at: 642 1231 642
Size: 589 3643
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 1817 1991 2382 2409 3754 1817
Size: 174 391 27 1345 2295
Fragments arranged by size:
2295 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 598 916 2057 2489 3236 3504 410
Size: 188 318 1141 432 747 268 1138
Fragments arranged by size:
1141 1138 747 432 318 268 188
EcoNI CCTnn'n_nnAGG
Cuts at: 485 485
Size: 4232
EcoO109I rG'GnC_Cy
Cuts at: 154 1371 154
Size: 1217 3015
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1666 1914 2936 3915 1666
Size: 248 1022 979 1983
Fragments arranged by size:
1983 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 649 681 2074 2104 2106 2136 571
Size: 32 46 32 1393 30 2 30 2667
Fragments arranged by size:
2667 1393 46 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MfeI C'AATT_G
Cuts at: 398 398
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 1993 1993
Size: 4232
MslI CAynn'nnrTG
Cuts at: 2221 2348 2221
Size: 127 4105
MspA1I CmG'CkG
Cuts at: 835 1343 1657 2017 3030 3275 835
Size: 508 314 360 1013 245 1792
Fragments arranged by size:
1792 1013 508 360 314 245
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 678 678
Size: 4232
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NspI r_CATG'y
Cuts at: 264 422 855 1285 1785 2692 264
Size: 158 433 430 500 907 1804
Fragments arranged by size:
1804 907 500 433 430 158
PciI A'CATG_T
Cuts at: 418 2688 418
Size: 2270 1962
PflMI CCAn_nnn'nTGG
Cuts at: 240 240
Size: 4232
PfoI T'CCnGG_A
Cuts at: 387 387
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PsiI TTA'TAA
Cuts at: 845 845
Size: 4232
PstI C_TGCA'G
Cuts at: 1279 1964 1279
Size: 685 3547
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 835 1657 2017 835
Size: 822 360 3050
Fragments arranged by size:
3050 822 360
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SexAI A'CCwGG_T
Cuts at: 661 661
Size: 4232
SfcI C'TryA_G
Cuts at: 33 708 1275 1960 2953 3144 33
Size: 675 567 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 567 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 1387 2302 2794 3056 1387
Size: 915 492 262 2563
Fragments arranged by size:
2563 915 492 262
SnaBI TAC'GTA
Cuts at: 1224 1224
Size: 4232
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 264 1285 1785 264
Size: 1021 500 2711
Fragments arranged by size:
2711 1021 500
StuI AGG'CCT
Cuts at: 753 753
Size: 4232
StyI C'CwwG_G
Cuts at: 678 1366 1720 678
Size: 688 354 3190
Fragments arranged by size:
3190 688 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2616 1815
Size: 133 459 209 3431
Fragments arranged by size:
3431 459 209 133
TatI w'GTAC_w
Cuts at: 927 964 1044 3747 927
Size: 37 80 2703 1412
Fragments arranged by size:
2703 1412 80 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1036 1727 1036
Size: 691 3541
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI