Restriction map for
pF1KSDA1820
(Circular) (Six-base) MAPSORT of: pF1KSDA1820.seq Check: 5810 from: 1 to: 8057
pF1KSDA1820 8057 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2581 4806 2581
Size: 2225 5832
AccI GT'mk_AC
Cuts at: 5095 5095
Size: 8057
Acc65I G'GTAC_C
Cuts at: 742 2251 5073 742
Size: 1509 2822 3726
Fragments arranged by size:
3726 2822 1509
AclI AA'CG_TT
Cuts at: 7897 7897
Size: 8057
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 988 1122 1360 2027 2154 4126 5882 6314
Size: 525 134 238 667 127 1972 1756 432
Cuts at: 6314 7061 7329 463
Size: 747 268 1191
Fragments arranged by size:
1972 1756 1191 747 667 525 432 268
238 134 127
AfeI AGC'GCT
Cuts at: 3550 5044 3550
Size: 1494 6563
AflIII A'CryG_T
Cuts at: 1814 5409 6513 7490 1814
Size: 3595 1104 977 2381
Fragments arranged by size:
3595 2381 1104 977
AgeI A'CCGG_T
Cuts at: 2630 6445 2630
Size: 3815 4242
AhdI GACnn_n'nnGTC
Cuts at: 1815 2234 2796 1815
Size: 419 562 7076
Fragments arranged by size:
7076 562 419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 8057
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1516 1548 1516
Size: 32 8025
AlwNI CAG_nnn'CTG
Cuts at: 258 672 702 1479 1482 2069 4670 4821 4843
Size: 414 30 777 3 587 2601 151 22
Cuts at: 4843 5362 6929 258
Size: 519 1567 1386
Fragments arranged by size:
2601 1567 1386 777 587 519 414 151
30 22 3
ApaI G_GGCC'C
Cuts at: 495 825 2914 3224 4491 495
Size: 330 2089 310 1267 4061
Fragments arranged by size:
4061 2089 1267 330 310
ApaLI G'TGCA_C
Cuts at: 3133 4995 6827 3133
Size: 1862 1832 4363
Fragments arranged by size:
4363 1862 1832
ApoI r'AATT_y
Cuts at: 5061 7410 7666 5061
Size: 2349 256 5452
Fragments arranged by size:
5452 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8057
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8057
AvaI C'yCGr_G
Cuts at: 294 900 1774 2545 2611 2767 3070 3148 4043
Size: 606 874 771 66 156 303 78 895
Cuts at: 4043 4556 4748 5077 294
Size: 513 192 329 3274
Fragments arranged by size:
3274 895 874 771 606 513 329 303
192 156 78 66
BamHI G'GATC_C
Cuts at: 4830 5082 4830
Size: 252 7805
BanI G'GyrC_C
Cuts at: 607 615 742 1013 2251 2923 3075 3174 3813
Size: 8 127 271 1238 672 152 99 639
Cuts at: 3813 4282 4452 5073 5487 5735 5770 607
Size: 469 170 621 414 248 35 2894
Fragments arranged by size:
2894 1238 672 639 621 469 414 271
248 170 152 127 99 35 8
BanII G_rGCy'C
Cuts at: 495 825 1601 2756 2872 2914 3009 3224 3255
Size: 330 776 1155 116 42 95 215 31
Cuts at: 3255 3281 3323 3502 4201 4229 4491 4925 5071
Size: 26 42 179 699 28 262 434 146
Cuts at: 5071 6101 495
Size: 1030 2451
Fragments arranged by size:
2451 1155 1030 776 699 434 330 262
215 179 146 116 95 42 42 31
28 26
BbeI G_GCGC'C
Cuts at: 611 3079 3817 4456 5491 5739 611
Size: 2468 738 639 1035 248 2929
Fragments arranged by size:
2929 2468 1035 738 639 248
BbsI GAAGACnn'nnnn_
Cuts at: 2195 3938 4075 4373 2195
Size: 1743 137 298 5879
Fragments arranged by size:
5879 1743 298 137
BbvCI CC'TCA_GC
Cuts at: 755 946 2726 4882 755
Size: 191 1780 2156 3930
Fragments arranged by size:
3930 2156 1780 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 118 152 4976 5010 118
Size: 34 4824 34 3165
Fragments arranged by size:
4824 3165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5955 6716 8057 5955
Size: 761 1341 5955
Fragments arranged by size:
5955 1341 761
BclI T'GATC_A
Cuts at: 5577 5577
Size: 8057
BglI GCCn_nnn'nGGC
Cuts at: 343 1853 3040 3497 3811 4045 4475 343
Size: 1510 1187 457 314 234 430 3925
Fragments arranged by size:
3925 1510 1187 457 430 314 234
BglII A'GATC_T
Cuts at: 3587 5572 7609 3587
Size: 1985 2037 4035
Fragments arranged by size:
4035 2037 1985
BlpI GC'TnA_GC
Cuts at: 238 1255 1921 2334 5169 238
Size: 1017 666 413 2835 3126
Fragments arranged by size:
3126 2835 1017 666 413
Bme1580I G_kGCm'C
Cuts at: 495 581 620 825 2611 2914 2926 3071 3137
Size: 86 39 205 1786 303 12 145 66
Cuts at: 3137 3177 3224 4285 4491 4692 4999 5682 5775
Size: 40 47 1061 206 201 307 683 93
Cuts at: 5775 6831 495
Size: 1056 1721
Fragments arranged by size:
1786 1721 1061 1056 683 307 303 206
205 201 145 93 86 66 47 40
39 12
BmgBI CAC'GTC
Cuts at: 642 642
Size: 8057
BmrI ACTGGGnnnn_n'
Cuts at: 759 3385 4243 5269 5350 5685 7829 759
Size: 2626 858 1026 81 335 2144 987
Fragments arranged by size:
2626 2144 1026 987 858 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1476 2754 2757 3537 4614 6424 1476
Size: 1278 3 780 1077 1810 3109
Fragments arranged by size:
3109 1810 1278 1077 780 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 1134 1246 1720 2074 2264 2452 2659 2993
Size: 261 112 474 354 190 188 207 334
Cuts at: 2993 3269 3429 3678 4534 4939 873
Size: 276 160 249 856 405 3991
Fragments arranged by size:
3991 856 474 405 354 334 276 261
249 207 190 188 160 112
Bpu10I CC'TnA_GC
Cuts at: 755 946 1678 2726 4882 755
Size: 191 732 1048 2156 3930
Fragments arranged by size:
3930 2156 1048 732 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4015 4132 4213 4840 5233 6112 6604 6902 4015
Size: 117 81 627 393 879 492 298 5170
Fragments arranged by size:
5170 879 627 492 393 298 117 81
BsaI GGTCTCn'nnnn_
Cuts at: 3696 4006 7761 3696
Size: 310 3755 3992
Fragments arranged by size:
3992 3755 310
BsaAI yAC'GTr
Cuts at: 5049 5410 5049
Size: 361 7696
BsaBI GATnn'nnATC
Cuts at: 6 5571 7608 6
Size: 5565 2037 455
Fragments arranged by size:
5565 2037 455
BsaHI Gr'CG_yC
Cuts at: 428 608 3032 3076 3814 4453 5488 5736 6438
Size: 180 2424 44 738 639 1035 248 702
Cuts at: 6438 7934 428
Size: 1496 551
Fragments arranged by size:
2424 1496 1035 738 702 639 551 248
180 44
BsaWI w'CCGG_w
Cuts at: 1109 2630 5246 5767 6445 6719 6866 7277 1109
Size: 1521 2616 521 678 274 147 411 1889
Fragments arranged by size:
2616 1889 1521 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 703 733 1861 1891 1933 1963 3535 3565 703
Size: 30 1128 30 42 30 1572 30 5195
Fragments arranged by size:
5195 1572 1128 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 484 771 783 786 2524 2578 2599 2730 2743
Size: 287 12 3 1738 54 21 131 13
Cuts at: 2743 2746 2845 2854 3646 3919 4479 4622 4645
Size: 3 99 9 792 273 560 143 23
Cuts at: 4645 484
Size: 3896
Fragments arranged by size:
3896 1738 792 560 287 273 143 131
99 54 23 21 13 12 9 3
3
BseYI C'CCAG_C
Cuts at: 583 662 857 1144 2014 2864 2907 3451 3526
Size: 79 195 287 870 850 43 544 75
Cuts at: 3526 4141 4237 4635 4649 4924 5482 6240 6817
Size: 615 96 398 14 275 558 758 577
Cuts at: 6817 7313 583
Size: 496 1327
Fragments arranged by size:
1327 870 850 758 615 577 558 544
496 398 287 275 195 96 79 75
43 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 2805 3198 3344 3773 4144 5009 622
Size: 2183 393 146 429 371 865 3670
Fragments arranged by size:
3670 2183 865 429 393 371 146
BsiEI CG_ry'CG
Cuts at: 104 249 3464 4990 5599 5645 6853 104
Size: 145 3215 1526 609 46 1208 1308
Fragments arranged by size:
3215 1526 1308 1208 609 145 46
BsiHKAI G_wGCw'C
Cuts at: 2756 3009 3137 4999 5071 5849 6039 6831 2756
Size: 253 128 1862 72 778 190 792 3982
Fragments arranged by size:
3982 1862 792 778 253 190 128 72
BsmI GAATG_Cn'
Cuts at: 759 759
Size: 8057
Bsp1286I G_dGCh'C
Cuts at: 495 581 620 825 1601 2611 2756 2872 2914
Size: 86 39 205 776 1010 145 116 42
Cuts at: 2914 2926 3009 3071 3137 3177 3224 3255 3281
Size: 12 83 62 66 40 47 31 26
Cuts at: 3281 3323 3502 4201 4229 4285 4491 4692 4925
Size: 42 179 699 28 56 206 201 233
Cuts at: 4925 4999 5071 5682 5775 5849 6039 6101 6831
Size: 74 72 611 93 74 190 62 730
Cuts at: 6831 495
Size: 1721
Fragments arranged by size:
1721 1010 776 730 699 611 233 206
205 201 190 179 145 116 93 86
83 74 74 72 66 62 62 56
47 42 42 40 39 31 28 26
12
BspEI T'CCGG_A
Cuts at: 1109 7277 1109
Size: 6168 1889
BspHI T'CATG_A
Cuts at: 4361 7233 4361
Size: 2872 5185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 1409 2581 4806 5107 5321 5623 6004 537
Size: 872 1172 2225 301 214 302 381 2590
Fragments arranged by size:
2590 2225 1172 872 381 302 301 214
BsrBI CCG'CTC
Cuts at: 50 1169 1181 1254 1583 1682 1866 1899 6349
Size: 1119 12 73 329 99 184 33 4450
Cuts at: 6349 6403 7887 8052 50
Size: 54 1484 165 55
Fragments arranged by size:
4450 1484 1119 329 184 165 99 73
55 54 33 12
BsrDI GCAATG_nn'
Cuts at: 713 2163 3891 3984 5969 713
Size: 1450 1728 93 1985 2801
Fragments arranged by size:
2801 1985 1728 1450 93
BsrFI r'CCGG_y
Cuts at: 248 408 1010 2630 2648 2860 3623 3773 3816
Size: 160 602 1620 18 212 763 150 43
Cuts at: 3816 6055 6236 6445 248
Size: 2239 181 209 1860
Fragments arranged by size:
2239 1860 1620 763 602 212 209 181
160 150 43 18
BssSI C'ACGA_G
Cuts at: 1528 6328 6686 1528
Size: 4800 358 2899
Fragments arranged by size:
4800 2899 358
BstAPI GCAn_nnn'nTGC
Cuts at: 258 396 702 853 1482 4670 4818 4821 5434
Size: 138 306 151 629 3188 148 3 613
Cuts at: 5434 258
Size: 2881
Fragments arranged by size:
3188 2881 629 613 306 151 148 138
3
BstBI TT'CG_AA
Cuts at: 110 6418 110
Size: 6308 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 811 1344 1732 1887 2430 811
Size: 533 388 155 543 6438
Fragments arranged by size:
6438 543 533 388 155
BstYI r'GATC_y
Cuts at: 1 1410 1955 3587 4342 4830 5082 5549 5572
Size: 1409 545 1632 755 488 252 467 23
Cuts at: 5572 5907 6153 7165 7251 7263 7609 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1632 1409 1012 755 545 488 467 449
346 335 252 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3914 3914
Size: 8057
BtgI C'CryG_G
Cuts at: 156 338 1172 1731 1909 2198 3103 4221 4491
Size: 182 834 559 178 289 905 1118 270
Cuts at: 4491 156
Size: 3722
Fragments arranged by size:
3722 1118 905 834 559 289 270 182
178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3066 6007 6188 6351 3066
Size: 2941 181 163 4772
Fragments arranged by size:
4772 2941 181 163
BtsI GCAGTG_nn'
Cuts at: 329 581 1563 2611 2652 2686 5300 329
Size: 252 982 1048 41 34 2614 3086
Fragments arranged by size:
3086 2614 1048 982 252 41 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3861 3896 4203 4238 4584 4619 3861
Size: 35 307 35 346 35 7299
Fragments arranged by size:
7299 346 307 35 35 35
DraI TTT'AAA
Cuts at: 5056 5056
Size: 8057
DrdI GACnn_nn'nnGTC
Cuts at: 1105 5763 6621 1105
Size: 4658 858 2541
Fragments arranged by size:
4658 2541 858
EaeI y'GGCC_r
Cuts at: 344 539 2084 2362 2618 3461 4953 5642 5816
Size: 195 1545 278 256 843 1492 689 174
Cuts at: 5816 6207 6234 7579 344
Size: 391 27 1345 822
Fragments arranged by size:
1545 1492 1345 843 822 689 391 278
256 195 174 27
EagI C'GGCC_G
Cuts at: 3461 5642 3461
Size: 2181 5876
EarI CTCTTCn'nnn_
Cuts at: 2978 3937 4456 4779 6080 6290 2978
Size: 959 519 323 1301 210 4745
Fragments arranged by size:
4745 1301 959 519 323 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3621 4840 4993 6265 6575 6721 7867 3621
Size: 1219 153 1272 310 146 1146 3811
Fragments arranged by size:
3811 1272 1219 1146 310 153 146
EcoICRI GAG'CTC
Cuts at: 2754 3007 5069 2754
Size: 253 2062 5742
Fragments arranged by size:
5742 2062 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 873 988 1122 1134 1246 1360 1720 2027
Size: 410 115 134 12 112 114 360 307
Cuts at: 2027 2074 2154 2264 2452 2659 2993 3269 3429
Size: 47 80 110 188 207 334 276 160
Cuts at: 3429 3678 4126 4534 4939 5882 6314 7061 7329
Size: 249 448 408 405 943 432 747 268
Cuts at: 7329 463
Size: 1191
Fragments arranged by size:
1191 943 747 448 432 410 408 405
360 334 307 276 268 249 207 188
160 134 115 114 112 110 80 47
12
EcoNI CCTnn'n_nnAGG
Cuts at: 1378 1933 2023 2434 3752 1378
Size: 555 90 411 1318 5683
Fragments arranged by size:
5683 1318 555 411 90
EcoO109I rG'GnC_Cy
Cuts at: 375 491 492 821 1265 1538 1692 1941 2121
Size: 116 1 329 444 273 154 249 180
Cuts at: 2121 2427 2514 2679 2715 2790 2808 2911 2989
Size: 306 87 165 36 75 18 103 78
Cuts at: 2989 3220 3221 3266 3611 3738 4101 4175 4425
Size: 231 1 45 345 127 363 74 250
Cuts at: 4425 4487 4667 5196 375
Size: 62 180 529 3236
Fragments arranged by size:
3236 529 444 363 345 329 306 273
250 249 231 180 180 165 154 127
116 103 87 78 75 74 62 45
36 18 1 1
EcoRI G'AATT_C
Cuts at: 5061 5061
Size: 8057
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2061 3822 6076 2061
Size: 1761 2254 4042
Fragments arranged by size:
4042 2254 1761
FspI TGC'GCA
Cuts at: 5838 5838
Size: 8057
HaeII r_GCGC'y
Cuts at: 314 611 1438 1928 2069 2348 3079 3552 3817
Size: 297 827 490 141 279 731 473 265
Cuts at: 3817 4171 4456 4743 5046 5491 5739 6761 7740
Size: 354 285 287 303 445 248 1022 979
Cuts at: 7740 314
Size: 631
Fragments arranged by size:
1022 979 827 731 631 490 473 445
354 303 297 287 285 279 265 248
141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 1768 1800 1801 1833 1861 1893 1933
Size: 32 1033 32 1 32 28 32 40
Cuts at: 1933 1965 2220 2252 2782 2814 3279 3311 3535
Size: 32 255 32 530 32 465 32 224
Cuts at: 3535 3567 3933 3965 5899 5929 5931 5961 703
Size: 32 366 32 1934 30 2 30 2799
Fragments arranged by size:
2799 1934 1033 530 465 366 255 224
40 32 32 32 32 32 32 32
32 32 32 30 30 28 2 1
HincII GTy'rAC
Cuts at: 1752 5096 1752
Size: 3344 4713
HindIII A'AGCT_T
Cuts at: 1997 3114 1997
Size: 1117 6940
KasI G'GCGC_C
Cuts at: 607 3075 3813 4452 5487 5735 607
Size: 2468 738 639 1035 248 2929
Fragments arranged by size:
2929 2468 1035 738 639 248
KpnI G_GTAC'C
Cuts at: 746 2255 5077 746
Size: 1509 2822 3726
Fragments arranged by size:
3726 2822 1509
MluI A'CGCG_T
Cuts at: 7490 7490
Size: 8057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1388 1487 3506 4235 4357 6728 6912 1388
Size: 99 2019 729 122 2371 184 2533
Fragments arranged by size:
2533 2371 2019 729 184 122 99
MscI TGG'CCA
Cuts at: 541 2086 2364 2620 5818 541
Size: 1545 278 256 3198 2780
Fragments arranged by size:
3198 2780 1545 278 256
MslI CAynn'nnrTG
Cuts at: 6046 6173 6046
Size: 127 7930
MspA1I CmG'CkG
Cuts at: 255 340 876 909 1212 1479 1614 1911 2142
Size: 85 536 33 303 267 135 297 231
Cuts at: 2142 2326 2338 2796 3729 4512 5168 5482 5842
Size: 184 12 458 933 783 656 314 360
Cuts at: 5842 6855 7100 255
Size: 1013 245 1212
Fragments arranged by size:
1212 1013 933 783 656 536 458 360
314 303 297 267 245 231 184 135
85 33 12
NaeI GCC'GGC
Cuts at: 410 1012 2650 2862 3625 6238 410
Size: 602 1638 212 763 2613 2229
Fragments arranged by size:
2613 2229 1638 763 602 212
NarI GG'CG_CC
Cuts at: 608 3076 3814 4453 5488 5736 608
Size: 2468 738 639 1035 248 2929
Fragments arranged by size:
2929 2468 1035 738 639 248
NcoI C'CATG_G
Cuts at: 156 4491 156
Size: 4335 3722
NgoMIV G'CCGG_C
Cuts at: 408 1010 2648 2860 3623 6236 408
Size: 602 1638 212 763 2613 2229
Fragments arranged by size:
2613 2229 1638 763 602 212
NspI r_CATG'y
Cuts at: 1654 1818 2787 4163 5110 5610 6517 1654
Size: 164 969 1376 947 500 907 3194
Fragments arranged by size:
3194 1376 969 947 907 500 164
PasI CC'CwG_GG
Cuts at: 738 2176 2521 3228 738
Size: 1438 345 707 5567
Fragments arranged by size:
5567 1438 707 345
PciI A'CATG_T
Cuts at: 1814 6513 1814
Size: 4699 3358
PflMI CCAn_nnn'nTGG
Cuts at: 4306 4306
Size: 8057
PfoI T'CCnGG_A
Cuts at: 431 1534 2312 2504 431
Size: 1103 778 192 5984
Fragments arranged by size:
5984 1103 778 192
PmeI GTTT'AAAC
Cuts at: 5056 5056
Size: 8057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4756 4788 7196 7228 4756
Size: 32 2408 32 5585
Fragments arranged by size:
5585 2408 32 32
PpuMI rG'GwC_Cy
Cuts at: 1538 1692 1941 2514 2679 2715 2790 3738 1538
Size: 154 249 573 165 36 75 948 5857
Fragments arranged by size:
5857 948 573 249 165 154 75 36
PsiI TTA'TAA
Cuts at: 278 278
Size: 8057
PspOMI G'GGCC_C
Cuts at: 491 821 2910 3220 4487 491
Size: 330 2089 310 1267 4061
Fragments arranged by size:
4061 2089 1267 330 310
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1222 1254 1222
Size: 32 8025
PstI C_TGCA'G
Cuts at: 336 401 534 707 1424 1570 1970 2606 4433
Size: 65 133 173 717 146 400 636 1827
Cuts at: 4433 5104 5789 336
Size: 671 685 2604
Fragments arranged by size:
2604 1827 717 685 671 636 400 173
146 133 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8057
PvuII CAG'CTG
Cuts at: 1479 2142 5482 5842 1479
Size: 663 3340 360 3694
Fragments arranged by size:
3694 3340 663 360
RsrII CG'GwC_CG
Cuts at: 6252 6252
Size: 8057
SacI G_AGCT'C
Cuts at: 2756 3009 5071 2756
Size: 253 2062 5742
Fragments arranged by size:
5742 2062 253
SacII CC_GC'GG
Cuts at: 341 1912 341
Size: 1571 6486
SalI G'TCGA_C
Cuts at: 5094 5094
Size: 8057
SanDI GG'GwC_CC
Cuts at: 3738 3738
Size: 8057
SapI GCTCTTCn'nnn_
Cuts at: 6080 6290 6080
Size: 210 7847
SbfI CC_TGCA'GG
Cuts at: 401 534 5104 401
Size: 133 4570 3354
Fragments arranged by size:
4570 3354 133
ScaI AGT'ACT
Cuts at: 1126 2737 7574 1126
Size: 1611 4837 1609
Fragments arranged by size:
4837 1611 1609
SexAI A'CCwGG_T
Cuts at: 3747 3839 3747
Size: 92 7965
SfcI C'TryA_G
Cuts at: 33 332 397 530 703 1191 1239 1420 1566
Size: 299 65 133 173 488 48 181 146
Cuts at: 1566 1966 2291 2602 2915 4429 4716 5100 5785
Size: 400 325 311 313 1514 287 384 685
Cuts at: 5785 6778 6969 33
Size: 993 191 1121
Fragments arranged by size:
1514 1121 993 685 488 400 384 325
313 311 299 287 191 181 173 146
133 65 48
SfoI GGC'GCC
Cuts at: 609 3077 3815 4454 5489 5737 609
Size: 2468 738 639 1035 248 2929
Fragments arranged by size:
2929 2468 1035 738 639 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8057
SmaI CCC'GGG
Cuts at: 902 2547 5079 902
Size: 1645 2532 3880
Fragments arranged by size:
3880 2532 1645
SmlI C'TyrA_G
Cuts at: 4030 4147 4228 4855 5212 6127 6619 6881 4030
Size: 117 81 627 357 915 492 262 5206
Fragments arranged by size:
5206 915 627 492 357 262 117 81
SnaBI TAC'GTA
Cuts at: 5049 5049
Size: 8057
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8057
SphI G_CATG'C
Cuts at: 1654 2787 5110 5610 1654
Size: 1133 2323 500 4101
Fragments arranged by size:
4101 2323 1133 500
StuI AGG'CCT
Cuts at: 395 2449 2778 3344 3912 4268 4408 395
Size: 2054 329 566 568 356 140 4044
Fragments arranged by size:
4044 2054 568 566 356 329 140
StyI C'CwwG_G
Cuts at: 156 266 1490 2162 2265 2423 2621 2804 2825
Size: 110 1224 672 103 158 198 183 21
Cuts at: 2825 2855 3434 3509 3971 4073 4491 5191 5545
Size: 30 579 75 462 102 418 700 354
Cuts at: 5545 156
Size: 2668
Fragments arranged by size:
2668 1224 700 672 579 462 418 354
198 183 158 110 103 102 75 30
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1366 2028 2491 2560 2712 2817 3447 3540 3554
Size: 662 463 69 152 105 630 93 14
Cuts at: 3554 5640 5773 6232 6441 1366
Size: 2086 133 459 209 2982
Fragments arranged by size:
2982 2086 662 630 463 459 209 152
133 105 93 69 14
TatI w'GTAC_w
Cuts at: 1124 2735 3292 4859 7572 1124
Size: 1611 557 1567 2713 1609
Fragments arranged by size:
2713 1611 1609 1567 557
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2644 2676 7574 7606 2644
Size: 32 4898 32 3095
Fragments arranged by size:
4898 3095 32 32
Tth111I GACn'n_nGTC
Cuts at: 5854 5854
Size: 8057
XbaI T'CTAG_A
Cuts at: 5088 5088
Size: 8057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 891 1341 2832 4509 5552 891
Size: 450 1491 1677 1043 3396
Fragments arranged by size:
3396 1677 1491 1043 450
XmaI C'CCGG_G
Cuts at: 900 2545 5077 900
Size: 1645 2532 3880
Fragments arranged by size:
3880 2532 1645
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PspXI SfiI SgrAI
SrfI SspI SwaI XhoI XmnI ZraI