Restriction map for
pF1KSDA1798
(Circular) (Six-base) MAPSORT of: pF1KSDA1798.seq Check: 5210 from: 1 to: 5477
pF1KSDA1798 5477 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 387 387
Size: 5477
AccI GT'mk_AC
Cuts at: 1265 1577 2515 1265
Size: 312 938 4227
Fragments arranged by size:
4227 938 312
Acc65I G'GTAC_C
Cuts at: 321 2493 321
Size: 2172 3305
AclI AA'CG_TT
Cuts at: 5317 5317
Size: 5477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1729 1766 3302 3734 4481 4749 460
Size: 1269 37 1536 432 747 268 1188
Fragments arranged by size:
1536 1269 1188 747 432 268 37
AflII C'TTAA_G
Cuts at: 1162 1923 1162
Size: 761 4716
AflIII A'CryG_T
Cuts at: 1168 2829 3933 4910 1168
Size: 1661 1104 977 1735
Fragments arranged by size:
1735 1661 1104 977
AgeI A'CCGG_T
Cuts at: 3865 3865
Size: 5477
AhdI GACnn_n'nnGTC
Cuts at: 2262 2262
Size: 5477
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 2096
Size: 32 5445
AlwNI CAG_nnn'CTG
Cuts at: 2782 4349 2782
Size: 1567 3910
ApaI G_GGCC'C
Cuts at: 1855 2398 1855
Size: 543 4934
ApaLI G'TGCA_C
Cuts at: 4247 4247
Size: 5477
ApoI r'AATT_y
Cuts at: 1141 2269 2408 2448 2481 4830 5086 1141
Size: 1128 139 40 33 2349 256 1532
Fragments arranged by size:
2349 1532 1128 256 139 40 33
AseI AT'TA_AT
Cuts at: 20 1771 20
Size: 1751 3726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
AvaI C'yCGr_G
Cuts at: 354 949 1806 2096 2497 354
Size: 595 857 290 401 3334
Fragments arranged by size:
3334 857 595 401 290
BamHI G'GATC_C
Cuts at: 453 945 2502 453
Size: 492 1557 3428
Fragments arranged by size:
3428 1557 492
BanI G'GyrC_C
Cuts at: 321 2092 2493 2907 3155 3190 321
Size: 1771 401 414 248 35 2608
Fragments arranged by size:
2608 1771 414 401 248 35
BanII G_rGCy'C
Cuts at: 252 361 1762 1855 2398 2491 3521 252
Size: 109 1401 93 543 93 1030 2208
Fragments arranged by size:
2208 1401 1030 543 109 93 93
BbeI G_GCGC'C
Cuts at: 2911 3159 2911
Size: 248 5229
BbsI GAAGACnn'nnnn_
Cuts at: 433 611 2045 433
Size: 178 1434 3865
Fragments arranged by size:
3865 1434 178
BciVI GTATCCnnnnn_n'
Cuts at: 991 3375 4136 5477 991
Size: 2384 761 1341 991
Fragments arranged by size:
2384 1341 991 761
BclI T'GATC_A
Cuts at: 1629 2997 1629
Size: 1368 4109
BglII A'GATC_T
Cuts at: 2992 5029 2992
Size: 2037 3440
BlpI GC'TnA_GC
Cuts at: 2589 2589
Size: 5477
Bme1580I G_kGCm'C
Cuts at: 205 1855 2097 2398 3102 3195 4251 205
Size: 1650 242 301 704 93 1056 1431
Fragments arranged by size:
1650 1431 1056 704 301 242 93
BmrI ACTGGGnnnn_n'
Cuts at: 440 1072 1356 2401 2689 2770 3105 5249 440
Size: 632 284 1045 288 81 335 2144 668
Fragments arranged by size:
2144 1045 668 632 335 288 284 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3844 3844
Size: 5477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 548 345
Size: 203 5274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1163 1276 1588 2653 3532 4024 4322 317
Size: 846 113 312 1065 879 492 298 1472
Fragments arranged by size:
1472 1065 879 846 492 312 298 113
BsaI GGTCTCn'nnnn_
Cuts at: 2006 5181 2006
Size: 3175 2302
BsaAI yAC'GTr
Cuts at: 2469 2830 2469
Size: 361 5116
BsaBI GATnn'nnATC
Cuts at: 6 575 2991 5028 6
Size: 569 2416 2037 455
Fragments arranged by size:
2416 2037 569 455
BsaHI Gr'CG_yC
Cuts at: 2908 3156 3858 5354 2908
Size: 248 702 1496 3031
Fragments arranged by size:
3031 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1001 2666 3187 3865 4139 4286 4697 1001
Size: 1665 521 678 274 147 411 1781
Fragments arranged by size:
1781 1665 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 859 1529 859
Size: 670 4807
BseYI C'CCAG_C
Cuts at: 824 2183 2902 3660 4237 4733 824
Size: 1359 719 758 577 496 1568
Fragments arranged by size:
1568 1359 758 719 577 496
BsiEI CG_ry'CG
Cuts at: 104 3019 3065 4273 104
Size: 2915 46 1208 1308
Fragments arranged by size:
2915 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 361 558 753 2254 2491 3269 3459 4251 361
Size: 197 195 1501 237 778 190 792 1587
Fragments arranged by size:
1587 1501 792 778 237 197 195 190
BsmI GAATG_Cn'
Cuts at: 1059 1059
Size: 5477
Bsp1286I G_dGCh'C
Cuts at: 205 252 361 558 753 1762 1855 2097 2254
Size: 47 109 197 195 1009 93 242 157
Cuts at: 2254 2398 2491 3102 3195 3269 3459 3521 4251
Size: 144 93 611 93 74 190 62 730
Cuts at: 4251 205
Size: 1431
Fragments arranged by size:
1431 1009 730 611 242 197 195 190
157 144 109 93 93 93 74 62
47
BspEI T'CCGG_A
Cuts at: 4697 4697
Size: 5477
BspHI T'CATG_A
Cuts at: 4653 4653
Size: 5477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 387 1515 2527 2741 3043 3424 387
Size: 1128 1012 214 302 381 2440
Fragments arranged by size:
2440 1128 1012 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3769 3823 5307 5472 50
Size: 3719 54 1484 165 55
Fragments arranged by size:
3719 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3389 3389
Size: 5477
BsrFI r'CCGG_y
Cuts at: 864 1099 3475 3656 3865 864
Size: 235 2376 181 209 2476
Fragments arranged by size:
2476 2376 235 209 181
BssSI C'ACGA_G
Cuts at: 3748 4106 3748
Size: 358 5119
BstAPI GCAn_nnn'nTGC
Cuts at: 795 2854 795
Size: 2059 3418
BstBI TT'CG_AA
Cuts at: 110 3838 110
Size: 3728 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 318 1081 1085 1406 318
Size: 763 4 321 4389
Fragments arranged by size:
4389 763 321 4
BstYI r'GATC_y
Cuts at: 1 453 621 945 2502 2969 2992 3327 3573
Size: 452 168 324 1557 467 23 335 246
Cuts at: 3573 4585 4671 4683 5029 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1557 1012 467 452 449 346 335 324
246 168 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 733 733
Size: 5477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3427 3608 3771 3427
Size: 181 163 5133
Fragments arranged by size:
5133 181 163
BtsI GCAGTG_nn'
Cuts at: 866 2720 866
Size: 1854 3623
DraI TTT'AAA
Cuts at: 2316 2476 2316
Size: 160 5317
DrdI GACnn_nn'nnGTC
Cuts at: 3183 4041 3183
Size: 858 4619
EaeI y'GGCC_r
Cuts at: 1101 1824 3062 3236 3627 3654 4999 1101
Size: 723 1238 174 391 27 1345 1579
Fragments arranged by size:
1579 1345 1238 723 391 174 27
EagI C'GGCC_G
Cuts at: 3062 3062
Size: 5477
EarI CTCTTCn'nnn_
Cuts at: 683 836 1998 2042 3500 3710 683
Size: 153 1162 44 1458 210 2450
Fragments arranged by size:
2450 1458 1162 210 153 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3685 3995 4141 5287 3685
Size: 310 146 1146 3875
Fragments arranged by size:
3875 1146 310 146
EcoICRI GAG'CTC
Cuts at: 359 2489 359
Size: 2130 3347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 460 548 1729 1766 3302 3734 4481 4749
Size: 115 88 1181 37 1536 432 747 268
Cuts at: 4749 345
Size: 1073
Fragments arranged by size:
1536 1181 1073 747 432 268 115 88
37
EcoNI CCTnn'n_nnAGG
Cuts at: 803 841 1121 803
Size: 38 280 5159
Fragments arranged by size:
5159 280 38
EcoO109I rG'GnC_Cy
Cuts at: 1852 2395 2616 1852
Size: 543 221 4713
Fragments arranged by size:
4713 543 221
EcoRI G'AATT_C
Cuts at: 1141 2448 2481 1141
Size: 1307 33 4137
Fragments arranged by size:
4137 1307 33
EcoRV GAT'ATC
Cuts at: 1071 1071
Size: 5477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1032 2012 3496 1032
Size: 980 1484 3013
Fragments arranged by size:
3013 1484 980
FspI TGC'GCA
Cuts at: 3258 3258
Size: 5477
HaeII r_GCGC'y
Cuts at: 2911 3159 4181 5160 2911
Size: 248 1022 979 3228
Fragments arranged by size:
3228 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 874 906 943 975 2248 2280 3319 3349 3351
Size: 32 37 32 1273 32 1039 30 2
Cuts at: 3351 3381 874
Size: 30 2970
Fragments arranged by size:
2970 1273 1039 37 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2516 2516
Size: 5477
HindIII A'AGCT_T
Cuts at: 1009 1252 1564 1778 1009
Size: 243 312 214 4708
Fragments arranged by size:
4708 312 243 214
KasI G'GCGC_C
Cuts at: 2907 3155 2907
Size: 248 5229
KpnI G_GTAC'C
Cuts at: 325 2497 325
Size: 2172 3305
MfeI C'AATT_G
Cuts at: 1146 1665 1146
Size: 519 4958
MluI A'CGCG_T
Cuts at: 4910 4910
Size: 5477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 616 2233 4148 4332 616
Size: 1617 1915 184 1761
Fragments arranged by size:
1915 1761 1617 184
MscI TGG'CCA
Cuts at: 1826 3238 1826
Size: 1412 4065
MslI CAynn'nnrTG
Cuts at: 316 402 1019 1652 2024 2425 3466 3593 316
Size: 86 617 633 372 401 1041 127 2200
Fragments arranged by size:
2200 1041 633 617 401 372 127 86
MspA1I CmG'CkG
Cuts at: 2137 2588 2902 3262 4275 4520 2137
Size: 451 314 360 1013 245 3094
Fragments arranged by size:
3094 1013 451 360 314 245
NaeI GCC'GGC
Cuts at: 866 3658 866
Size: 2792 2685
NarI GG'CG_CC
Cuts at: 2908 3156 2908
Size: 248 5229
NgoMIV G'CCGG_C
Cuts at: 864 3656 864
Size: 2792 2685
NspI r_CATG'y
Cuts at: 1172 2023 2530 3030 3937 1172
Size: 851 507 500 907 2712
Fragments arranged by size:
2712 907 851 507 500
PciI A'CATG_T
Cuts at: 1168 3933 1168
Size: 2765 2712
PflMI CCAn_nnn'nTGG
Cuts at: 314 314
Size: 5477
PmeI GTTT'AAAC
Cuts at: 2476 2476
Size: 5477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 4616
Size: 32 5445
PspOMI G'GGCC_C
Cuts at: 1851 2394 1851
Size: 543 4934
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1419 1451 1419
Size: 32 5445
PstI C_TGCA'G
Cuts at: 2442 2524 3209 2442
Size: 82 685 4710
Fragments arranged by size:
4710 685 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5477
PvuII CAG'CTG
Cuts at: 2137 2902 3262 2137
Size: 765 360 4352
Fragments arranged by size:
4352 765 360
RsrII CG'GwC_CG
Cuts at: 3672 3672
Size: 5477
SacI G_AGCT'C
Cuts at: 361 2491 361
Size: 2130 3347
SalI G'TCGA_C
Cuts at: 2514 2514
Size: 5477
SapI GCTCTTCn'nnn_
Cuts at: 1998 3500 3710 1998
Size: 1502 210 3765
Fragments arranged by size:
3765 1502 210
SbfI CC_TGCA'GG
Cuts at: 2524 2524
Size: 5477
ScaI AGT'ACT
Cuts at: 4994 4994
Size: 5477
SexAI A'CCwGG_T
Cuts at: 1443 1443
Size: 5477
SfcI C'TryA_G
Cuts at: 33 1712 2438 2520 3205 4198 4389 33
Size: 1679 726 82 685 993 191 1121
Fragments arranged by size:
1679 1121 993 726 685 191 82
SfoI GGC'GCC
Cuts at: 2909 3157 2909
Size: 248 5229
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
SmaI CCC'GGG
Cuts at: 1808 2098 2499 1808
Size: 290 401 4786
Fragments arranged by size:
4786 401 290
SmlI C'TyrA_G
Cuts at: 332 1162 1178 1255 1567 1923 2632 3547 4039
Size: 830 16 77 312 356 709 915 492
Cuts at: 4039 4301 332
Size: 262 1508
Fragments arranged by size:
1508 915 830 709 492 356 312 262
77 16
SnaBI TAC'GTA
Cuts at: 2469 2469
Size: 5477
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5477
SphI G_CATG'C
Cuts at: 2023 2530 3030 2023
Size: 507 500 4470
Fragments arranged by size:
4470 507 500
StyI C'CwwG_G
Cuts at: 2611 2965 2611
Size: 354 5123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1038 1073 1091 2120 2279 3060 3193 3652 3861
Size: 35 18 1029 159 781 133 459 209
Cuts at: 3861 1038
Size: 2654
Fragments arranged by size:
2654 1029 781 459 209 159 133 35
18
TatI w'GTAC_w
Cuts at: 780 965 4992 780
Size: 185 4027 1265
Fragments arranged by size:
4027 1265 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4994 5026 4994
Size: 32 5445
Tth111I GACn'n_nGTC
Cuts at: 3274 3274
Size: 5477
XbaI T'CTAG_A
Cuts at: 2508 2508
Size: 5477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 315 383 2972 315
Size: 68 2589 2820
Fragments arranged by size:
2820 2589 68
XmaI C'CCGG_G
Cuts at: 1806 2096 2497 1806
Size: 290 401 4786
Fragments arranged by size:
4786 401 290
XmnI GAAnn'nnTTC
Cuts at: 473 874 2342 473
Size: 401 1468 3608
Fragments arranged by size:
3608 1468 401
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AlfI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI
CspCI DraIII FseI FspAI HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspXI SacII SanDI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI