Restriction map for
pF1KSDA1792
(Circular) (Six-base) MAPSORT of: pF1KSDA1792.seq Check: 9985 from: 1 to: 5431
pF1KSDA1792 5431 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1547 1555 1547
Size: 8 5423
AccI GT'mk_AC
Cuts at: 2467 2467
Size: 5431
Acc65I G'GTAC_C
Cuts at: 1081 2445 1081
Size: 1364 4067
AclI AA'CG_TT
Cuts at: 5271 5271
Size: 5431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1720 1939 3254 3686 4435 4703 1720
Size: 219 1315 432 749 268 2448
Fragments arranged by size:
2448 1315 749 432 268 219
AflII C'TTAA_G
Cuts at: 1657 1657
Size: 5431
AflIII A'CryG_T
Cuts at: 2781 3887 4864 2781
Size: 1106 977 3348
Fragments arranged by size:
3348 1106 977
AgeI A'CCGG_T
Cuts at: 3819 3819
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1225 1225
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 540 1176 1369 2074 2734 4303 540
Size: 636 193 705 660 1569 1668
Fragments arranged by size:
1668 1569 705 660 636 193
ApaI G_GGCC'C
Cuts at: 1844 1844
Size: 5431
ApaLI G'TGCA_C
Cuts at: 4201 4201
Size: 5431
ApoI r'AATT_y
Cuts at: 361 1126 1277 1471 1516 1785 1798 2433 4784
Size: 765 151 194 45 269 13 635 2351
Cuts at: 4784 5040 361
Size: 256 752
Fragments arranged by size:
2351 765 752 635 269 256 194 151
45 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 5431
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5431
AvaI C'yCGr_G
Cuts at: 2449 2449
Size: 5431
AvrII C'CTAG_G
Cuts at: 901 901
Size: 5431
BamHI G'GATC_C
Cuts at: 2454 2454
Size: 5431
BanI G'GyrC_C
Cuts at: 1081 2445 2859 3107 3142 1081
Size: 1364 414 248 35 3370
Fragments arranged by size:
3370 1364 414 248 35
BanII G_rGCy'C
Cuts at: 409 640 1844 2443 3473 409
Size: 231 1204 599 1030 2367
Fragments arranged by size:
2367 1204 1030 599 231
BbeI G_GCGC'C
Cuts at: 2863 3111 2863
Size: 248 5183
BbvCI CC'TCA_GC
Cuts at: 1271 1271
Size: 5431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 155 189 2284 2318 155
Size: 34 2095 34 3268
Fragments arranged by size:
3268 2095 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1017 3327 4090 5431 1017
Size: 2310 763 1341 1017
Fragments arranged by size:
2310 1341 1017 763
BclI T'GATC_A
Cuts at: 743 1061 2949 743
Size: 318 1888 3225
Fragments arranged by size:
3225 1888 318
BfrBI ATG'CAT
Cuts at: 520 520
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 1556 1951 1556
Size: 395 5036
BglII A'GATC_T
Cuts at: 738 2944 4983 738
Size: 2206 2039 1186
Fragments arranged by size:
2206 2039 1186
BlpI GC'TnA_GC
Cuts at: 634 2541 634
Size: 1907 3524
Bme1580I G_kGCm'C
Cuts at: 348 1844 2182 3054 3147 4205 348
Size: 1496 338 872 93 1058 1574
Fragments arranged by size:
1574 1496 1058 872 338 93
BmrI ACTGGGnnnn_n'
Cuts at: 1839 2346 2641 2722 3057 5203 1839
Size: 507 295 81 335 2146 2067
Fragments arranged by size:
2146 2067 507 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3796 3796
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 583 897 1464 1843 2241 239
Size: 344 314 567 379 398 3429
Fragments arranged by size:
3429 567 398 379 344 314
Bpu10I CC'TnA_GC
Cuts at: 1271 1631 1271
Size: 360 5071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 590 1999 2605 3484 3978 4276 124
Size: 466 1409 606 879 494 298 1279
Fragments arranged by size:
1409 1279 879 606 494 466 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 1819 5135 805
Size: 1014 3316 1101
Fragments arranged by size:
3316 1101 1014
BsaAI yAC'GTr
Cuts at: 1227 2421 2782 1227
Size: 1194 361 3876
Fragments arranged by size:
3876 1194 361
BsaBI GATnn'nnATC
Cuts at: 6 2943 4982 6
Size: 2937 2039 455
Fragments arranged by size:
2937 2039 455
BsaHI Gr'CG_yC
Cuts at: 2860 3108 3812 5308 2860
Size: 248 704 1496 2983
Fragments arranged by size:
2983 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2618 3139 3819 4093 4240 4651 2618
Size: 521 680 274 147 411 3398
Fragments arranged by size:
3398 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1087 1259 1087
Size: 172 5259
BseYI C'CCAG_C
Cuts at: 402 669 1554 1833 2854 3612 4191 4687 402
Size: 267 885 279 1021 758 579 496 1146
Fragments arranged by size:
1146 1021 885 758 579 496 279 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 2260 215
Size: 2045 3386
BsiEI CG_ry'CG
Cuts at: 104 2971 3017 4227 104
Size: 2867 46 1210 1308
Fragments arranged by size:
2867 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 640 2443 3221 3411 4205 640
Size: 1803 778 190 794 1866
Fragments arranged by size:
1866 1803 794 778 190
BsmI GAATG_Cn'
Cuts at: 1365 1365
Size: 5431
Bsp1286I G_dGCh'C
Cuts at: 348 409 640 1844 2182 2443 3054 3147 3221
Size: 61 231 1204 338 261 611 93 74
Cuts at: 3221 3411 3473 4205 348
Size: 190 62 732 1574
Fragments arranged by size:
1574 1204 732 611 338 261 231 190
93 74 62 61
BspEI T'CCGG_A
Cuts at: 4651 4651
Size: 5431
BspHI T'CATG_A
Cuts at: 1244 2391 4607 1244
Size: 1147 2216 2068
Fragments arranged by size:
2216 2068 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1547 1555 2479 2693 2995 3376 1547
Size: 8 924 214 302 381 3602
Fragments arranged by size:
3602 924 381 302 214 8
BsrBI CCG'CTC
Cuts at: 50 3721 3775 5261 5426 50
Size: 3671 54 1486 165 55
Fragments arranged by size:
3671 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 394 2142 3341 394
Size: 1748 1199 2484
Fragments arranged by size:
2484 1748 1199
BsrFI r'CCGG_y
Cuts at: 1950 3427 3608 3819 1950
Size: 1477 181 211 3562
Fragments arranged by size:
3562 1477 211 181
BssSI C'ACGA_G
Cuts at: 3700 4060 3700
Size: 360 5071
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 2806 1369
Size: 1437 3994
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5431
BstXI CCAn_nnnn'nTGG
Cuts at: 1092 2094 2166 1092
Size: 1002 72 4357
Fragments arranged by size:
4357 1002 72
BstYI r'GATC_y
Cuts at: 1 738 948 2262 2454 2921 2944 3279 3525
Size: 737 210 1314 192 467 23 335 246
Cuts at: 3525 4539 4625 4637 4983 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1314 1014 737 467 449 346 335 246
210 192 86 23 12
BtgI C'CryG_G
Cuts at: 125 2087 2159 125
Size: 1962 72 3397
Fragments arranged by size:
3397 1962 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3379 3560 3723 3379
Size: 181 163 5087
Fragments arranged by size:
5087 181 163
BtsI GCAGTG_nn'
Cuts at: 348 1370 2672 348
Size: 1022 1302 3107
Fragments arranged by size:
3107 1302 1022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 408 443 408
Size: 35 5396
DraI TTT'AAA
Cuts at: 911 1104 2428 911
Size: 193 1324 3914
Fragments arranged by size:
3914 1324 193
DrdI GACnn_nn'nnGTC
Cuts at: 1897 3135 3995 1897
Size: 1238 860 3333
Fragments arranged by size:
3333 1238 860
EaeI y'GGCC_r
Cuts at: 3014 3188 3579 3606 4953 3014
Size: 174 391 27 1347 3492
Fragments arranged by size:
3492 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3014 3014
Size: 5431
EarI CTCTTCn'nnn_
Cuts at: 321 428 2281 3452 3662 321
Size: 107 1853 1171 210 2090
Fragments arranged by size:
2090 1853 1171 210 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3637 3949 4095 5241 3637
Size: 312 146 1146 3827
Fragments arranged by size:
3827 1146 312 146
EcoICRI GAG'CTC
Cuts at: 638 2441 638
Size: 1803 3628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 583 897 1464 1720 1843 1939 2241 3254
Size: 344 314 567 256 123 96 302 1013
Cuts at: 3254 3686 4435 4703 239
Size: 432 749 268 967
Fragments arranged by size:
1013 967 749 567 432 344 314 302
268 256 123 96
EcoNI CCTnn'n_nnAGG
Cuts at: 1657 1657
Size: 5431
EcoO109I rG'GnC_Cy
Cuts at: 249 1014 1840 1841 2568 249
Size: 765 826 1 727 3112
Fragments arranged by size:
3112 826 765 727 1
EcoRI G'AATT_C
Cuts at: 361 1277 1516 1785 1798 2433 361
Size: 916 239 269 13 635 3359
Fragments arranged by size:
3359 916 635 269 239 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3448 3448
Size: 5431
FspI TGC'GCA
Cuts at: 3210 3210
Size: 5431
HaeII r_GCGC'y
Cuts at: 2863 3111 4135 5114 2863
Size: 248 1024 979 3180
Fragments arranged by size:
3180 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1254 1286 1857 1889 3271 3301 3303 3333 1254
Size: 32 571 32 1382 30 2 30 3352
Fragments arranged by size:
3352 1382 571 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1914 2468 1914
Size: 554 4877
HindIII A'AGCT_T
Cuts at: 758 758
Size: 5431
HpaI GTT'AAC
Cuts at: 1914 1914
Size: 5431
KasI G'GCGC_C
Cuts at: 2859 3107 2859
Size: 248 5183
KpnI G_GTAC'C
Cuts at: 1085 2449 1085
Size: 1364 4067
MluI A'CGCG_T
Cuts at: 4864 4864
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2075 4102 4286 2075
Size: 2027 184 3220
Fragments arranged by size:
3220 2027 184
MscI TGG'CCA
Cuts at: 3190 3190
Size: 5431
MslI CAynn'nnrTG
Cuts at: 1225 2164 3418 3545 1225
Size: 939 1254 127 3111
Fragments arranged by size:
3111 1254 939 127
MspA1I CmG'CkG
Cuts at: 402 702 2110 2298 2540 2854 3214 4229 4474
Size: 300 1408 188 242 314 360 1015 245
Cuts at: 4474 402
Size: 1359
Fragments arranged by size:
1408 1359 1015 360 314 300 245 242
188
NaeI GCC'GGC
Cuts at: 1952 3610 1952
Size: 1658 3773
NarI GG'CG_CC
Cuts at: 2860 3108 2860
Size: 248 5183
NcoI C'CATG_G
Cuts at: 125 2087 2159 125
Size: 1962 72 3397
Fragments arranged by size:
3397 1962 72
NdeI CA'TA_TG
Cuts at: 301 1862 301
Size: 1561 3870
NgoMIV G'CCGG_C
Cuts at: 1950 3608 1950
Size: 1658 3773
NruI TCG'CGA
Cuts at: 3792 3792
Size: 5431
NsiI A_TGCA'T
Cuts at: 522 522
Size: 5431
NspI r_CATG'y
Cuts at: 471 1987 2196 2482 2982 3891 471
Size: 1516 209 286 500 909 2011
Fragments arranged by size:
2011 1516 909 500 286 209
PasI CC'CwG_GG
Cuts at: 413 1287 413
Size: 874 4557
PciI A'CATG_T
Cuts at: 3887 3887
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2428 2428
Size: 5431
PmlI CAC'GTG
Cuts at: 1227 1227
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4570 4602 4570
Size: 32 5399
PpuMI rG'GwC_Cy
Cuts at: 249 1014 249
Size: 765 4666
PsiI TTA'TAA
Cuts at: 1451 1451
Size: 5431
PspOMI G'GGCC_C
Cuts at: 1840 1840
Size: 5431
PstI C_TGCA'G
Cuts at: 651 2476 3161 651
Size: 1825 685 2921
Fragments arranged by size:
2921 1825 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5431
PvuII CAG'CTG
Cuts at: 402 702 2110 2298 2854 3214 402
Size: 300 1408 188 556 360 2619
Fragments arranged by size:
2619 1408 556 360 300 188
RsrII CG'GwC_CG
Cuts at: 3624 3624
Size: 5431
SacI G_AGCT'C
Cuts at: 640 2443 640
Size: 1803 3628
SalI G'TCGA_C
Cuts at: 2466 2466
Size: 5431
SapI GCTCTTCn'nnn_
Cuts at: 3452 3662 3452
Size: 210 5221
SbfI CC_TGCA'GG
Cuts at: 2476 2476
Size: 5431
ScaI AGT'ACT
Cuts at: 1113 1576 1728 4948 1113
Size: 463 152 3220 1596
Fragments arranged by size:
3220 1596 463 152
SfcI C'TryA_G
Cuts at: 33 481 647 2472 3157 4152 4343 33
Size: 448 166 1825 685 995 191 1121
Fragments arranged by size:
1825 1121 995 685 448 191 166
SfoI GGC'GCC
Cuts at: 2861 3109 2861
Size: 248 5183
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5431
SmaI CCC'GGG
Cuts at: 2451 2451
Size: 5431
SmlI C'TyrA_G
Cuts at: 139 605 1657 1978 2584 3499 3993 4255 139
Size: 466 1052 321 606 915 494 262 1315
Fragments arranged by size:
1315 1052 915 606 494 466 321 262
SnaBI TAC'GTA
Cuts at: 2421 2421
Size: 5431
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5431
SphI G_CATG'C
Cuts at: 2196 2482 2982 2196
Size: 286 500 4645
Fragments arranged by size:
4645 500 286
SspI AAT'ATT
Cuts at: 1200 1200
Size: 5431
StyI C'CwwG_G
Cuts at: 125 901 2087 2159 2268 2563 2917 125
Size: 776 1186 72 109 295 354 2639
Fragments arranged by size:
2639 1186 776 354 295 109 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 365 426 1473 3012 3145 3604 3815 365
Size: 61 1047 1539 133 459 211 1981
Fragments arranged by size:
1981 1539 1047 459 211 133 61
TatI w'GTAC_w
Cuts at: 391 1111 1574 1726 4946 391
Size: 720 463 152 3220 876
Fragments arranged by size:
3220 876 720 463 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4948 4980 4948
Size: 32 5399
Tth111I GACn'n_nGTC
Cuts at: 3226 3226
Size: 5431
XbaI T'CTAG_A
Cuts at: 2460 2460
Size: 5431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1551 2248 2924 1551
Size: 697 676 4058
Fragments arranged by size:
4058 697 676
XmaI C'CCGG_G
Cuts at: 2449 2449
Size: 5431
XmnI GAAnn'nnTTC
Cuts at: 1281 2401 1281
Size: 1120 4311
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NsiI NspI PasI
PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI BaeI BbsI
BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI MfeI
NheI NotI PacI PflMI PfoI PshAI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI
XhoI ZraI