Restriction map for
pF1KSDA1787
(Circular) (Six-base) MAPSORT of: pF1KSDA1787.seq Check: 8837 from: 1 to: 7823
pF1KSDA1787 7823 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3639 3718 3978 4119 3639
Size: 79 260 141 7343
Fragments arranged by size:
7343 260 141 79
AccI GT'mk_AC
Cuts at: 4861 4861
Size: 7823
Acc65I G'GTAC_C
Cuts at: 4839 4839
Size: 7823
AclI AA'CG_TT
Cuts at: 7663 7663
Size: 7823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 741 830 2504 2943 3869 4719 5648 6080
Size: 227 89 1674 439 926 850 929 432
Cuts at: 6080 6827 7095 514
Size: 747 268 1242
Fragments arranged by size:
1674 1242 929 926 850 747 439 432
268 227 89
AfeI AGC'GCT
Cuts at: 449 449
Size: 7823
AflIII A'CryG_T
Cuts at: 378 3326 3839 5175 6279 7256 378
Size: 2948 513 1336 1104 977 945
Fragments arranged by size:
2948 1336 1104 977 945 513
AgeI A'CCGG_T
Cuts at: 6211 6211
Size: 7823
AhdI GACnn_n'nnGTC
Cuts at: 2442 2442
Size: 7823
AleI CACnn'nnGTG
Cuts at: 2173 3583 2173
Size: 1410 6413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1819 1819
Size: 7823
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 988 1020 988
Size: 32 7791
AlwNI CAG_nnn'CTG
Cuts at: 573 622 1678 2203 2854 3310 3642 4018 4447
Size: 49 1056 525 651 456 332 376 429
Cuts at: 4447 5128 6695 573
Size: 681 1567 1701
Fragments arranged by size:
1701 1567 1056 681 651 525 456 429
376 332 49
ApaI G_GGCC'C
Cuts at: 199 2101 2391 3830 199
Size: 1902 290 1439 4192
Fragments arranged by size:
4192 1902 1439 290
ApaLI G'TGCA_C
Cuts at: 720 2618 4520 6593 720
Size: 1898 1902 2073 1950
Fragments arranged by size:
2073 1950 1902 1898
ApoI r'AATT_y
Cuts at: 1159 1396 2494 4827 7176 7432 1159
Size: 237 1098 2333 2349 256 1550
Fragments arranged by size:
2349 2333 1550 1098 256 237
AseI AT'TA_AT
Cuts at: 20 1475 20
Size: 1455 6368
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7823
AvaI C'yCGr_G
Cuts at: 790 1633 2121 2697 3079 3520 4286 4529 4689
Size: 843 488 576 382 441 766 243 160
Cuts at: 4689 4843 790
Size: 154 3770
Fragments arranged by size:
3770 843 766 576 488 441 382 243
160 154
BamHI G'GATC_C
Cuts at: 4848 4848
Size: 7823
BanI G'GyrC_C
Cuts at: 1330 1375 3406 3630 3853 4839 5253 5501 5536
Size: 45 2031 224 223 986 414 248 35
Cuts at: 5536 1330
Size: 3617
Fragments arranged by size:
3617 2031 986 414 248 224 223 45
35
BanII G_rGCy'C
Cuts at: 157 199 505 797 986 1006 1803 2101 2269
Size: 42 306 292 189 20 797 298 168
Cuts at: 2269 2391 3128 3830 4837 5867 157
Size: 122 737 702 1007 1030 2113
Fragments arranged by size:
2113 1030 1007 797 737 702 306 298
292 189 168 122 42 20
BbeI G_GCGC'C
Cuts at: 5257 5505 5257
Size: 248 7575
BbsI GAAGACnn'nnnn_
Cuts at: 375 818 2492 2949 3142 3783 375
Size: 443 1674 457 193 641 4415
Fragments arranged by size:
4415 1674 641 457 443 193
BbvCI CC'TCA_GC
Cuts at: 2974 2981 2974
Size: 7 7816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2907 2941 4126 4160 4202 4236 4501 4535 2907
Size: 34 1185 34 42 34 265 34 6195
Fragments arranged by size:
6195 1185 265 42 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3162 5721 6482 7823 3162
Size: 2559 761 1341 3162
Fragments arranged by size:
3162 2559 1341 761
BclI T'GATC_A
Cuts at: 5343 5343
Size: 7823
BglI GCCn_nnn'nGGC
Cuts at: 1669 3485 3723 3851 1669
Size: 1816 238 128 5641
Fragments arranged by size:
5641 1816 238 128
BglII A'GATC_T
Cuts at: 3809 5338 7375 3809
Size: 1529 2037 4257
Fragments arranged by size:
4257 2037 1529
BlpI GC'TnA_GC
Cuts at: 1136 1789 4935 1136
Size: 653 3146 4024
Fragments arranged by size:
4024 3146 653
Bme1580I G_kGCm'C
Cuts at: 199 724 1378 2006 2101 2120 2391 2614 2622
Size: 525 654 628 95 19 271 223 8
Cuts at: 2622 3635 3753 3830 3847 3944 4524 4794 5448
Size: 1013 118 77 17 97 580 270 654
Cuts at: 5448 5541 6597 199
Size: 93 1056 1425
Fragments arranged by size:
1425 1056 1013 654 654 628 580 525
271 270 223 118 97 95 93 77
19 17 8
BmgBI CAC'GTC
Cuts at: 2184 2184
Size: 7823
BmrI ACTGGGnnnn_n'
Cuts at: 278 452 1510 1964 2842 3419 5035 5116 5451
Size: 174 1058 454 878 577 1616 81 335
Cuts at: 5451 7595 278
Size: 2144 506
Fragments arranged by size:
2144 1616 1058 878 577 506 454 335
174 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6190 6190
Size: 7823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 1306 1888 2180 2483 3169 3412 3550 4366
Size: 868 582 292 303 686 243 138 816
Cuts at: 4366 438
Size: 3895
Fragments arranged by size:
3895 868 816 686 582 303 292 243
138
Bpu10I CC'TnA_GC
Cuts at: 213 746 1000 1679 2974 2981 4007 4459 4646
Size: 533 254 679 1295 7 1026 452 187
Cuts at: 4646 213
Size: 3390
Fragments arranged by size:
3390 1295 1026 679 533 452 254 187
7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 2005 3790 4771 4999 5878 6370 6668 925
Size: 1080 1785 981 228 879 492 298 2080
Fragments arranged by size:
2080 1785 1080 981 879 492 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 3808 4582 7527 3808
Size: 774 2945 4104
Fragments arranged by size:
4104 2945 774
BsaAI yAC'GTr
Cuts at: 3842 4815 5176 3842
Size: 973 361 6489
Fragments arranged by size:
6489 973 361
BsaBI GATnn'nnATC
Cuts at: 6 1222 3271 5337 7374 6
Size: 1216 2049 2066 2037 455
Fragments arranged by size:
2066 2049 2037 1216 455
BsaHI Gr'CG_yC
Cuts at: 1510 2126 2366 5254 5502 6204 7700 1510
Size: 616 240 2888 248 702 1496 1633
Fragments arranged by size:
2888 1633 1496 702 616 248 240
BsaWI w'CCGG_w
Cuts at: 3128 5012 5533 6211 6485 6632 7043 3128
Size: 1884 521 678 274 147 411 3908
Fragments arranged by size:
3908 1884 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 988 1018 3399 3429 988
Size: 30 2381 30 5382
Fragments arranged by size:
5382 2381 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 571 2920 3409 3466 3484 4265 4516 4517 4549
Size: 2349 489 57 18 781 251 1 32
Cuts at: 4549 4774 571
Size: 225 3620
Fragments arranged by size:
3620 2349 781 489 251 225 57 32
18 1
BseYI C'CCAG_C
Cuts at: 199 622 867 1302 2607 3042 3219 3410 3635
Size: 423 245 435 1305 435 177 191 225
Cuts at: 3635 3944 4097 4351 4434 4579 4628 5248 6006
Size: 309 153 254 83 145 49 620 758
Cuts at: 6006 6583 7079 199
Size: 577 496 943
Fragments arranged by size:
1305 943 758 620 577 496 435 435
423 309 254 245 225 191 177 153
145 83 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 2275 2641 2824 3667 4335 241
Size: 2034 366 183 843 668 3729
Fragments arranged by size:
3729 2034 843 668 366 183
BsiEI CG_ry'CG
Cuts at: 104 402 883 5365 5411 6619 104
Size: 298 481 4482 46 1208 1308
Fragments arranged by size:
4482 1308 1208 481 298 46
BsiHKAI G_wGCw'C
Cuts at: 724 1006 1644 2622 3128 4524 4837 5615 5805
Size: 282 638 978 506 1396 313 778 190
Cuts at: 5805 6597 724
Size: 792 1950
Fragments arranged by size:
1950 1396 978 792 778 638 506 313
282 190
BsiWI C'GTAC_G
Cuts at: 301 3571 301
Size: 3270 4553
BsmBI CGTCTCn'nnnn_
Cuts at: 880 1600 4247 880
Size: 720 2647 4456
Fragments arranged by size:
4456 2647 720
Bsp1286I G_dGCh'C
Cuts at: 157 199 505 724 797 986 1006 1378 1644
Size: 42 306 219 73 189 20 372 266
Cuts at: 1644 1803 2006 2101 2120 2269 2391 2614 2622
Size: 159 203 95 19 149 122 223 8
Cuts at: 2622 3128 3635 3753 3830 3847 3944 4524 4794
Size: 506 507 118 77 17 97 580 270
Cuts at: 4794 4837 5448 5541 5615 5805 5867 6597 157
Size: 43 611 93 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 580 507 506 372 306
270 266 223 219 203 190 189 159
149 122 118 97 95 93 77 74
73 62 43 42 20 19 17 8
BspEI T'CCGG_A
Cuts at: 3128 7043 3128
Size: 3915 3908
BspHI T'CATG_A
Cuts at: 1086 1178 1337 6999 1086
Size: 92 159 5662 1910
Fragments arranged by size:
5662 1910 159 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2282 2663 3019 3639 3718 3978 4119 4307 4432
Size: 381 356 620 79 260 141 188 125
Cuts at: 4432 4873 5087 5389 5770 2282
Size: 441 214 302 381 4335
Fragments arranged by size:
4335 620 441 381 381 356 302 260
214 188 141 125 79
BsrBI CCG'CTC
Cuts at: 50 209 422 1346 6115 6169 7653 7818 50
Size: 159 213 924 4769 54 1484 165 55
Fragments arranged by size:
4769 1484 924 213 165 159 55 54
BsrDI GCAATG_nn'
Cuts at: 3314 4401 5735 3314
Size: 1087 1334 5402
Fragments arranged by size:
5402 1334 1087
BsrFI r'CCGG_y
Cuts at: 1011 1142 2779 3436 5821 6002 6211 1011
Size: 131 1637 657 2385 181 209 2623
Fragments arranged by size:
2623 2385 1637 657 209 181 131
BssHII G'CGCG_C
Cuts at: 3713 3713
Size: 7823
BssSI C'ACGA_G
Cuts at: 504 686 1729 2916 3384 6094 6452 504
Size: 182 1043 1187 468 2710 358 1875
Fragments arranged by size:
2710 1875 1187 1043 468 358 182
BstAPI GCAn_nnn'nTGC
Cuts at: 2929 4447 5200 2929
Size: 1518 753 5552
Fragments arranged by size:
5552 1518 753
BstBI TT'CG_AA
Cuts at: 110 6184 110
Size: 6074 1749
BstEII G'GTnAC_C
Cuts at: 1269 1423 1560 1269
Size: 154 137 7532
Fragments arranged by size:
7532 154 137
BstXI CCAn_nnnn'nTGG
Cuts at: 465 1293 2175 3147 3390 3585 465
Size: 828 882 972 243 195 4703
Fragments arranged by size:
4703 972 882 828 243 195
BstYI r'GATC_y
Cuts at: 1 1030 1217 3342 3809 4848 5315 5338 5673
Size: 1029 187 2125 467 1039 467 23 335
Cuts at: 5673 5919 6931 7017 7029 7375 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2125 1039 1029 1012 467 467 449 346
335 246 187 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1747 2216 2253 1747
Size: 469 37 7317
Fragments arranged by size:
7317 469 37
BtgI C'CryG_G
Cuts at: 125 339 485 2786 3360 3537 3783 125
Size: 214 146 2301 574 177 246 4165
Fragments arranged by size:
4165 2301 574 246 214 177 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 386 5773 5954 6117 386
Size: 5387 181 163 2092
Fragments arranged by size:
5387 2092 181 163
BtsI GCAGTG_nn'
Cuts at: 486 1559 1739 2813 2836 2930 3447 3985 4297
Size: 1073 180 1074 23 94 517 538 312
Cuts at: 4297 5066 486
Size: 769 3243
Fragments arranged by size:
3243 1074 1073 769 538 517 312 180
94 23
ClaI AT'CG_AT
Cuts at: 1484 1484
Size: 7823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2863 2898 3297 3332 2863
Size: 35 399 35 7354
Fragments arranged by size:
7354 399 35 35
DraI TTT'AAA
Cuts at: 4822 4822
Size: 7823
DraIII CAC_nnn'GTG
Cuts at: 1782 1782
Size: 7823
DrdI GACnn_nn'nnGTC
Cuts at: 406 2371 5529 6387 406
Size: 1965 3158 858 1842
Fragments arranged by size:
3158 1965 1842 858
EaeI y'GGCC_r
Cuts at: 880 2151 2673 2745 3201 3575 3587 5408 5582
Size: 1271 522 72 456 374 12 1821 174
Cuts at: 5582 5973 6000 7345 880
Size: 391 27 1345 1358
Fragments arranged by size:
1821 1358 1345 1271 522 456 391 374
174 72 27 12
EagI C'GGCC_G
Cuts at: 880 5408 880
Size: 4528 3295
EarI CTCTTCn'nnn_
Cuts at: 1696 3002 5846 6056 1696
Size: 1306 2844 210 3463
Fragments arranged by size:
3463 2844 1306 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1588 1636 2326 4193 4600 6031 6341 6487 7633
Size: 48 690 1867 407 1431 310 146 1146
Cuts at: 7633 1588
Size: 1778
Fragments arranged by size:
1867 1778 1431 1146 690 407 310 146
48
EcoICRI GAG'CTC
Cuts at: 1004 3126 4835 1004
Size: 2122 1709 3992
Fragments arranged by size:
3992 2122 1709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 514 741 830 1306 1888 2180 2483 2504
Size: 76 227 89 476 582 292 303 21
Cuts at: 2504 2943 3169 3412 3550 3869 4366 4719 5648
Size: 439 226 243 138 319 497 353 929
Cuts at: 5648 6080 6827 7095 438
Size: 432 747 268 1166
Fragments arranged by size:
1166 929 747 582 497 476 439 432
353 319 303 292 268 243 227 226
138 89 76 21
EcoNI CCTnn'n_nnAGG
Cuts at: 749 4010 4490 749
Size: 3261 480 4082
Fragments arranged by size:
4082 3261 480
EcoO109I rG'GnC_Cy
Cuts at: 166 195 196 570 836 1100 1751 1940 2097
Size: 29 1 374 266 264 651 189 157
Cuts at: 2097 2098 2388 3076 3298 4617 4693 4962 166
Size: 1 290 688 222 1319 76 269 3027
Fragments arranged by size:
3027 1319 688 651 374 290 269 266
264 222 189 157 76 29 1 1
EcoRI G'AATT_C
Cuts at: 1159 1396 2494 4827 1159
Size: 237 1098 2333 4155
Fragments arranged by size:
4155 2333 1098 237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5842 5842
Size: 7823
FspI TGC'GCA
Cuts at: 1831 2578 5604 1831
Size: 747 3026 4050
Fragments arranged by size:
4050 3026 747
FspAI rTGC'GCAy
Cuts at: 1831 1831
Size: 7823
HaeII r_GCGC'y
Cuts at: 276 451 1143 1627 1686 2690 5257 5505 6527
Size: 175 692 484 59 1004 2567 248 1022
Cuts at: 6527 7506 276
Size: 979 593
Fragments arranged by size:
2567 1022 1004 979 692 593 484 248
175 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 1231 1263 2428 2460 4452 4484 4609
Size: 32 670 32 1165 32 1992 32 125
Cuts at: 4609 4641 5665 5695 5697 5727 529
Size: 32 1024 30 2 30 2625
Fragments arranged by size:
2625 1992 1165 1024 670 125 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 411 4862 411
Size: 4451 3372
HindIII A'AGCT_T
Cuts at: 1231 1231
Size: 7823
KasI G'GCGC_C
Cuts at: 5253 5501 5253
Size: 248 7575
KpnI G_GTAC'C
Cuts at: 4843 4843
Size: 7823
MfeI C'AATT_G
Cuts at: 3371 3371
Size: 7823
MluI A'CGCG_T
Cuts at: 378 7256 378
Size: 6878 945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 251 533 1091 1475 2405 3320 6494 6678 251
Size: 282 558 384 930 915 3174 184 1396
Fragments arranged by size:
3174 1396 930 915 558 384 282 184
MscI TGG'CCA
Cuts at: 2153 2747 3589 5584 2153
Size: 594 842 1995 4392
Fragments arranged by size:
4392 1995 842 594
MslI CAynn'nnrTG
Cuts at: 1336 1339 2173 2533 2629 3145 3277 3583 3899
Size: 3 834 360 96 516 132 306 316
Cuts at: 3899 3905 5812 5939 1336
Size: 6 1907 127 3220
Fragments arranged by size:
3220 1907 834 516 360 316 306 132
127 96 6 3
MspA1I CmG'CkG
Cuts at: 203 245 2289 2441 2935 3158 3362 3639 4405
Size: 42 2044 152 494 223 204 277 766
Cuts at: 4405 4632 4705 4934 5248 5608 6621 6866 203
Size: 227 73 229 314 360 1013 245 1160
Fragments arranged by size:
2044 1160 1013 766 494 360 314 277
245 229 227 223 204 152 73 42
NaeI GCC'GGC
Cuts at: 1144 6004 1144
Size: 4860 2963
NarI GG'CG_CC
Cuts at: 5254 5502 5254
Size: 248 7575
NcoI C'CATG_G
Cuts at: 125 2786 3537 125
Size: 2661 751 4411
Fragments arranged by size:
4411 2661 751
NgoMIV G'CCGG_C
Cuts at: 1142 6002 1142
Size: 4860 2963
NspI r_CATG'y
Cuts at: 4876 5376 6283 4876
Size: 500 907 6416
Fragments arranged by size:
6416 907 500
PasI CC'CwG_GG
Cuts at: 3100 3100
Size: 7823
PciI A'CATG_T
Cuts at: 6279 6279
Size: 7823
PflMI CCAn_nnn'nTGG
Cuts at: 345 2854 3627 3642 4370 345
Size: 2509 773 15 728 3798
Fragments arranged by size:
3798 2509 773 728 15
PfoI T'CCnGG_A
Cuts at: 415 1988 3527 4613 415
Size: 1573 1539 1086 3625
Fragments arranged by size:
3625 1573 1539 1086
PmeI GTTT'AAAC
Cuts at: 4822 4822
Size: 7823
PmlI CAC'GTG
Cuts at: 3842 3842
Size: 7823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 988 1020 6962 6994 988
Size: 32 5942 32 1817
Fragments arranged by size:
5942 1817 32 32
PpuMI rG'GwC_Cy
Cuts at: 570 836 1100 1940 3076 4617 4693 570
Size: 266 264 840 1136 1541 76 3700
Fragments arranged by size:
3700 1541 1136 840 266 264 76
PshAI GACnn'nnGTC
Cuts at: 222 1567 222
Size: 1345 6478
PspOMI G'GGCC_C
Cuts at: 195 2097 2387 3826 195
Size: 1902 290 1439 4192
Fragments arranged by size:
4192 1902 1439 290
PspXI vC'TCGA_Gb
Cuts at: 790 3520 790
Size: 2730 5093
PstI C_TGCA'G
Cuts at: 846 3306 4870 5555 846
Size: 2460 1564 685 3114
Fragments arranged by size:
3114 2460 1564 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7823
PvuII CAG'CTG
Cuts at: 2289 3158 3639 5248 5608 2289
Size: 869 481 1609 360 4504
Fragments arranged by size:
4504 1609 869 481 360
RsrII CG'GwC_CG
Cuts at: 6018 6018
Size: 7823
SacI G_AGCT'C
Cuts at: 1006 3128 4837 1006
Size: 2122 1709 3992
Fragments arranged by size:
3992 2122 1709
SacII CC_GC'GG
Cuts at: 3363 3363
Size: 7823
SalI G'TCGA_C
Cuts at: 4860 4860
Size: 7823
SanDI GG'GwC_CC
Cuts at: 1100 3076 4617 4693 1100
Size: 1976 1541 76 4230
Fragments arranged by size:
4230 1976 1541 76
SapI GCTCTTCn'nnn_
Cuts at: 1696 3002 5846 6056 1696
Size: 1306 2844 210 3463
Fragments arranged by size:
3463 2844 1306 210
SbfI CC_TGCA'GG
Cuts at: 4870 4870
Size: 7823
ScaI AGT'ACT
Cuts at: 1390 2880 7340 1390
Size: 1490 4460 1873
Fragments arranged by size:
4460 1873 1490
SexAI A'CCwGG_T
Cuts at: 2228 2228
Size: 7823
SfcI C'TryA_G
Cuts at: 33 842 3302 4866 5551 6544 6735 33
Size: 809 2460 1564 685 993 191 1121
Fragments arranged by size:
2460 1564 1121 993 809 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3485 3485
Size: 7823
SfoI GGC'GCC
Cuts at: 5255 5503 5255
Size: 248 7575
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7823
SgrAI Cr'CCGG_yG
Cuts at: 1142 1142
Size: 7823
SmaI CCC'GGG
Cuts at: 2123 3081 4288 4691 4845 2123
Size: 958 1207 403 154 5101
Fragments arranged by size:
5101 1207 958 403 154
SmlI C'TyrA_G
Cuts at: 790 904 2020 3520 3805 4750 4978 5893 6385
Size: 114 1116 1500 285 945 228 915 492
Cuts at: 6385 6647 790
Size: 262 1966
Fragments arranged by size:
1966 1500 1116 945 915 492 285 262
228 114
SnaBI TAC'GTA
Cuts at: 4815 4815
Size: 7823
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7823
SphI G_CATG'C
Cuts at: 4876 5376 4876
Size: 500 7323
StuI AGG'CCT
Cuts at: 677 1025 677
Size: 348 7475
StyI C'CwwG_G
Cuts at: 125 797 821 2645 2682 2786 2966 3537 4044
Size: 672 24 1824 37 104 180 571 507
Cuts at: 4044 4710 4957 5311 125
Size: 666 247 354 2637
Fragments arranged by size:
2637 1824 672 666 571 507 354 247
180 104 37 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 630 739 1291 1741 1900 2579 3229 3424 4439
Size: 109 552 450 159 679 650 195 1015
Cuts at: 4439 5406 5539 5998 6207 630
Size: 967 133 459 209 2246
Fragments arranged by size:
2246 1015 967 679 650 552 459 450
209 195 159 133 109
TatI w'GTAC_w
Cuts at: 1388 2878 7338 1388
Size: 1490 4460 1873
Fragments arranged by size:
4460 1873 1490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 533 565 2526 2558 3399 3431 7340 7372 533
Size: 32 1961 32 841 32 3909 32 984
Fragments arranged by size:
3909 1961 984 841 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5620 5620
Size: 7823
XbaI T'CTAG_A
Cuts at: 2901 4854 2901
Size: 1953 5870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 1702 2784 5318 234
Size: 1468 1082 2534 2739
Fragments arranged by size:
2739 2534 1468 1082
XhoI C'TCGA_G
Cuts at: 790 3520 790
Size: 2730 5093
XmaI C'CCGG_G
Cuts at: 2121 3079 4286 4689 4843 2121
Size: 958 1207 403 154 5101
Fragments arranged by size:
5101 1207 958 403 154
XmnI GAAnn'nnTTC
Cuts at: 2830 2830
Size: 7823
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BfrBI BmtI BsmI
BsrGI BstZ17I EcoRV FseI HpaI NdeI NheI NotI
NruI NsiI PacI PsiI PsrI SrfI SspI SwaI
ZraI