Restriction map for
pF1KSDA1759
(Circular) (Six-base) MAPSORT of: pF1KSDA1759.seq Check: 3456 from: 1 to: 6053
pF1KSDA1759 6053 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2021 2508 2021
Size: 487 5566
AccI GT'mk_AC
Cuts at: 180 2900 3091 180
Size: 2720 191 3142
Fragments arranged by size:
3142 2720 191
Acc65I G'GTAC_C
Cuts at: 3069 3069
Size: 6053
AclI AA'CG_TT
Cuts at: 5893 5893
Size: 6053
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1326 2755 2975 3878 4310 5057 5325 1326
Size: 1429 220 903 432 747 268 2054
Fragments arranged by size:
2054 1429 903 747 432 268 220
AflIII A'CryG_T
Cuts at: 3405 4509 5486 3405
Size: 1104 977 3972
Fragments arranged by size:
3972 1104 977
AgeI A'CCGG_T
Cuts at: 4441 4441
Size: 6053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1933 2317 1933
Size: 384 5669
AlwNI CAG_nnn'CTG
Cuts at: 304 403 558 1216 1656 2454 2523 2656 3358
Size: 99 155 658 440 798 69 133 702
Cuts at: 3358 4925 304
Size: 1567 1432
Fragments arranged by size:
1567 1432 798 702 658 440 155 133
99 69
ApaI G_GGCC'C
Cuts at: 169 410 2005 2644 2748 2859 3032 169
Size: 241 1595 639 104 111 173 3190
Fragments arranged by size:
3190 1595 639 241 173 111 104
ApaLI G'TGCA_C
Cuts at: 4823 4823
Size: 6053
ApoI r'AATT_y
Cuts at: 2923 3057 5406 5662 2923
Size: 134 2349 256 3314
Fragments arranged by size:
3314 2349 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 6053
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6053
AvaI C'yCGr_G
Cuts at: 161 223 293 706 2371 2846 3073 161
Size: 62 70 413 1665 475 227 3141
Fragments arranged by size:
3141 1665 475 413 227 70 62
BamHI G'GATC_C
Cuts at: 3078 3078
Size: 6053
BanI G'GyrC_C
Cuts at: 173 796 1119 1511 2079 3069 3483 3731 3766
Size: 623 323 392 568 990 414 248 35
Cuts at: 3766 173
Size: 2460
Fragments arranged by size:
2460 990 623 568 414 392 323 248
35
BanII G_rGCy'C
Cuts at: 169 410 859 920 1880 2005 2159 2276 2530
Size: 241 449 61 960 125 154 117 254
Cuts at: 2530 2644 2748 2815 2859 3032 3067 4097 169
Size: 114 104 67 44 173 35 1030 2125
Fragments arranged by size:
2125 1030 960 449 254 241 173 154
125 117 114 104 67 61 44 35
BbeI G_GCGC'C
Cuts at: 3487 3735 3487
Size: 248 5805
BbsI GAAGACnn'nnnn_
Cuts at: 234 745 998 1775 2481 234
Size: 511 253 777 706 3806
Fragments arranged by size:
3806 777 706 511 253
BbvCI CC'TCA_GC
Cuts at: 1360 2290 2636 1360
Size: 930 346 4777
Fragments arranged by size:
4777 930 346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 307 308 341 368 402 274
Size: 33 1 33 27 34 5925
Fragments arranged by size:
5925 34 33 33 27 1
BciVI GTATCCnnnnn_n'
Cuts at: 3951 4712 6053 3951
Size: 761 1341 3951
Fragments arranged by size:
3951 1341 761
BclI T'GATC_A
Cuts at: 3573 3573
Size: 6053
BglI GCCn_nnn'nGGC
Cuts at: 1552 1661 2434 2752 1552
Size: 109 773 318 4853
Fragments arranged by size:
4853 773 318 109
BglII A'GATC_T
Cuts at: 3568 5605 3568
Size: 2037 4016
BlpI GC'TnA_GC
Cuts at: 145 509 1426 3165 145
Size: 364 917 1739 3033
Fragments arranged by size:
3033 1739 917 364
Bme1580I G_kGCm'C
Cuts at: 169 176 410 626 799 927 1557 2005 2644
Size: 7 234 216 173 128 630 448 639
Cuts at: 2644 2748 2859 3032 3678 3771 4827 169
Size: 104 111 173 646 93 1056 1395
Fragments arranged by size:
1395 1056 646 639 630 448 234 216
173 173 128 111 104 93 7
BmgBI CAC'GTC
Cuts at: 1801 1801
Size: 6053
BmrI ACTGGGnnnn_n'
Cuts at: 202 620 1874 2492 2640 3265 3346 3681 5825
Size: 418 1254 618 148 625 81 335 2144
Cuts at: 5825 202
Size: 430
Fragments arranged by size:
2144 1254 625 618 430 418 335 148
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 563 1563 1884 2133 4420 563
Size: 1000 321 249 2287 2196
Fragments arranged by size:
2287 2196 1000 321 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 436 758 1057 1658 1704 1778 1801 2816
Size: 138 322 299 601 46 74 23 1015
Cuts at: 2816 298
Size: 3535
Fragments arranged by size:
3535 1015 601 322 299 138 74 46
23
Bpu10I CC'TnA_GC
Cuts at: 731 1360 1874 2270 2290 2636 731
Size: 629 514 396 20 346 4148
Fragments arranged by size:
4148 629 514 396 346 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 2251 2914 3229 4108 4600 4898 170
Size: 2081 663 315 879 492 298 1325
Fragments arranged by size:
2081 1325 879 663 492 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 220 1332 5757 220
Size: 1112 4425 516
Fragments arranged by size:
4425 1112 516
BsaAI yAC'GTr
Cuts at: 3045 3406 3045
Size: 361 5692
BsaBI GATnn'nnATC
Cuts at: 6 3567 5604 6
Size: 3561 2037 455
Fragments arranged by size:
3561 2037 455
BsaHI Gr'CG_yC
Cuts at: 3484 3732 4434 5930 3484
Size: 248 702 1496 3607
Fragments arranged by size:
3607 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3242 3763 4441 4715 4862 5273 3242
Size: 521 678 274 147 411 4022
Fragments arranged by size:
4022 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1174 1575 1765 1939 1969 1986 2248 2251 2254
Size: 401 190 174 30 17 262 3 3
Cuts at: 2254 1174
Size: 4973
Fragments arranged by size:
4973 401 262 190 174 30 17 3
3
BseYI C'CCAG_C
Cuts at: 633 850 999 1522 1680 1965 1984 2024 2095
Size: 217 149 523 158 285 19 40 71
Cuts at: 2095 2429 2447 2523 2577 3478 4236 4813 5309
Size: 334 18 76 54 901 758 577 496
Cuts at: 5309 633
Size: 1377
Fragments arranged by size:
1377 901 758 577 523 496 334 285
217 158 149 76 71 54 40 19
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 398 412 1099 2012 2927 332
Size: 66 14 687 913 915 3458
Fragments arranged by size:
3458 915 913 687 66 14
BsiEI CG_ry'CG
Cuts at: 104 3595 3641 4849 104
Size: 3491 46 1208 1308
Fragments arranged by size:
3491 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2159 2360 3067 3845 4035 4827 2159
Size: 201 707 778 190 792 3385
Fragments arranged by size:
3385 792 778 707 201 190
BsmI GAATG_Cn'
Cuts at: 2898 2898
Size: 6053
BsmBI CGTCTCn'nnnn_
Cuts at: 2777 2777
Size: 6053
Bsp1286I G_dGCh'C
Cuts at: 169 176 410 626 799 859 920 927 1557
Size: 7 234 216 173 60 61 7 630
Cuts at: 1557 1880 2005 2159 2276 2360 2530 2644 2748
Size: 323 125 154 117 84 170 114 104
Cuts at: 2748 2815 2859 3032 3067 3678 3771 3845 4035
Size: 67 44 173 35 611 93 74 190
Cuts at: 4035 4097 4827 169
Size: 62 730 1395
Fragments arranged by size:
1395 730 630 611 323 234 216 190
173 173 170 154 125 117 114 104
93 84 74 67 62 61 60 44
35 7 7
BspEI T'CCGG_A
Cuts at: 5273 5273
Size: 6053
BspHI T'CATG_A
Cuts at: 1025 5229 1025
Size: 4204 1849
BspMI ACCTGCnnnn'nnnn_
Cuts at: 238 2021 2508 2664 3103 3317 3619 4000 238
Size: 1783 487 156 439 214 302 381 2291
Fragments arranged by size:
2291 1783 487 439 381 302 214 156
BsrBI CCG'CTC
Cuts at: 50 282 745 1863 2462 4345 4399 5883 6048
Size: 232 463 1118 599 1883 54 1484 165
Cuts at: 6048 50
Size: 55
Fragments arranged by size:
1883 1484 1118 599 463 232 165 55
54
BsrDI GCAATG_nn'
Cuts at: 475 700 1053 2190 2609 3965 475
Size: 225 353 1137 419 1356 2563
Fragments arranged by size:
2563 1356 1137 419 353 225
BsrFI r'CCGG_y
Cuts at: 1256 1437 4051 4232 4441 1256
Size: 181 2614 181 209 2868
Fragments arranged by size:
2868 2614 209 181 181
BssSI C'ACGA_G
Cuts at: 430 1556 4324 4682 430
Size: 1126 2768 358 1801
Fragments arranged by size:
2768 1801 1126 358
BstAPI GCAn_nnn'nTGC
Cuts at: 981 2656 3430 981
Size: 1675 774 3604
Fragments arranged by size:
3604 1675 774
BstBI TT'CG_AA
Cuts at: 110 4414 110
Size: 4304 1749
BstEII G'GTnAC_C
Cuts at: 2008 2008
Size: 6053
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2048 2032
Size: 16 6037
BstYI r'GATC_y
Cuts at: 1 435 3001 3078 3545 3568 3903 4149 5161
Size: 434 2566 77 467 23 335 246 1012
Cuts at: 5161 5247 5259 5605 1
Size: 86 12 346 449
Fragments arranged by size:
2566 1012 467 449 434 346 335 246
86 77 23 12
BstZ17I GTA'TAC
Cuts at: 181 181
Size: 6053
Bsu36I CC'TnA_GG
Cuts at: 1922 2054 1922
Size: 132 5921
BtgI C'CryG_G
Cuts at: 125 2616 125
Size: 2491 3562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 861 4003 4184 4347 861
Size: 3142 181 163 2567
Fragments arranged by size:
3142 2567 181 163
BtsI GCAGTG_nn'
Cuts at: 358 937 2221 2581 3296 358
Size: 579 1284 360 715 3115
Fragments arranged by size:
3115 1284 715 579 360
DraI TTT'AAA
Cuts at: 3052 3052
Size: 6053
DraIII CAC_nnn'GTG
Cuts at: 1804 1804
Size: 6053
DrdI GACnn_nn'nnGTC
Cuts at: 1819 3759 4617 1819
Size: 1940 858 3255
Fragments arranged by size:
3255 1940 858
EaeI y'GGCC_r
Cuts at: 612 908 1728 3638 3812 4203 4230 5575 612
Size: 296 820 1910 174 391 27 1345 1090
Fragments arranged by size:
1910 1345 1090 820 391 296 174 27
EagI C'GGCC_G
Cuts at: 3638 3638
Size: 6053
EarI CTCTTCn'nnn_
Cuts at: 1027 1345 1593 1642 1955 2680 2885 4076 4286
Size: 318 248 49 313 725 205 1191 210
Cuts at: 4286 1027
Size: 2794
Fragments arranged by size:
2794 1191 725 318 313 248 210 205
49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4261 4571 4717 5863 4261
Size: 310 146 1146 4451
Fragments arranged by size:
4451 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2157 3065 2157
Size: 908 5145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 436 758 1057 1326 1658 1704 1778 1801
Size: 138 322 299 269 332 46 74 23
Cuts at: 1801 2755 2816 2975 3878 4310 5057 5325 298
Size: 954 61 159 903 432 747 268 1026
Fragments arranged by size:
1026 954 903 747 432 332 322 299
269 268 159 138 74 61 46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2233 2515 2648 2233
Size: 282 133 5638
Fragments arranged by size:
5638 282 133
EcoO109I rG'GnC_Cy
Cuts at: 165 166 289 400 407 1132 1346 1561 2001
Size: 1 123 111 7 725 214 215 440
Cuts at: 2001 2002 2640 2641 2653 2744 2855 2856 3029
Size: 1 638 1 12 91 111 1 173
Cuts at: 3029 3192 165
Size: 163 3026
Fragments arranged by size:
3026 725 638 440 215 214 173 163
123 111 111 91 12 7 1 1
1 1
EcoRI G'AATT_C
Cuts at: 3057 3057
Size: 6053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4072 4072
Size: 6053
FspI TGC'GCA
Cuts at: 334 3834 334
Size: 3500 2553
HaeII r_GCGC'y
Cuts at: 3487 3735 4757 5736 3487
Size: 248 1022 979 3804
Fragments arranged by size:
3804 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2986 3018 3895 3925 3927 3957 2986
Size: 32 877 30 2 30 5082
Fragments arranged by size:
5082 877 32 30 30 2
HincII GTy'rAC
Cuts at: 356 455 2403 3092 356
Size: 99 1948 689 3317
Fragments arranged by size:
3317 1948 689 99
KasI G'GCGC_C
Cuts at: 3483 3731 3483
Size: 248 5805
KpnI G_GTAC'C
Cuts at: 3073 3073
Size: 6053
MfeI C'AATT_G
Cuts at: 2761 2761
Size: 6053
MluI A'CGCG_T
Cuts at: 5486 5486
Size: 6053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2761 2963 4724 4908 2606
Size: 155 202 1761 184 3751
Fragments arranged by size:
3751 1761 202 184 155
MscI TGG'CCA
Cuts at: 910 3814 910
Size: 2904 3149
MslI CAynn'nnrTG
Cuts at: 1259 2353 2482 4042 4169 1259
Size: 1094 129 1560 127 3143
Fragments arranged by size:
3143 1560 1094 129 127
MspA1I CmG'CkG
Cuts at: 465 672 850 1364 1386 1474 1817 2319 2433
Size: 207 178 514 22 88 343 502 114
Cuts at: 2433 3164 3478 3838 4851 5096 465
Size: 731 314 360 1013 245 1422
Fragments arranged by size:
1422 1013 731 514 502 360 343 314
245 207 178 114 88 22
NaeI GCC'GGC
Cuts at: 1439 4234 1439
Size: 2795 3258
NarI GG'CG_CC
Cuts at: 3484 3732 3484
Size: 248 5805
NcoI C'CATG_G
Cuts at: 125 125
Size: 6053
NgoMIV G'CCGG_C
Cuts at: 1437 4232 1437
Size: 2795 3258
NspI r_CATG'y
Cuts at: 1546 2382 2728 3106 3606 4513 1546
Size: 836 346 378 500 907 3086
Fragments arranged by size:
3086 907 836 500 378 346
PasI CC'CwG_GG
Cuts at: 169 403 801 2818 169
Size: 234 398 2017 3404
Fragments arranged by size:
3404 2017 398 234
PciI A'CATG_T
Cuts at: 4509 4509
Size: 6053
PfoI T'CCnGG_A
Cuts at: 275 1563 1894 2159 275
Size: 1288 331 265 4169
Fragments arranged by size:
4169 1288 331 265
PmeI GTTT'AAAC
Cuts at: 3052 3052
Size: 6053
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5192 5224 5192
Size: 32 6021
PpuMI rG'GwC_Cy
Cuts at: 1132 1346 1561 2653 1132
Size: 214 215 1092 4532
Fragments arranged by size:
4532 1092 215 214
PspOMI G'GGCC_C
Cuts at: 165 406 2001 2640 2744 2855 3028 165
Size: 241 1595 639 104 111 173 3190
Fragments arranged by size:
3190 1595 639 241 173 111 104
PspXI vC'TCGA_Gb
Cuts at: 706 706
Size: 6053
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1181 1213 1181
Size: 32 6021
PstI C_TGCA'G
Cuts at: 722 1385 1749 2387 2519 2649 3100 3785 722
Size: 663 364 638 132 130 451 685 2990
Fragments arranged by size:
2990 685 663 638 451 364 132 130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6053
PvuII CAG'CTG
Cuts at: 465 672 850 1364 1386 1474 2319 2433 3478
Size: 207 178 514 22 88 845 114 1045
Cuts at: 3478 3838 465
Size: 360 2680
Fragments arranged by size:
2680 1045 845 514 360 207 178 114
88 22
RsrII CG'GwC_CG
Cuts at: 4248 4248
Size: 6053
SacI G_AGCT'C
Cuts at: 2159 3067 2159
Size: 908 5145
SalI G'TCGA_C
Cuts at: 3090 3090
Size: 6053
SapI GCTCTTCn'nnn_
Cuts at: 1955 2680 2885 4076 4286 1955
Size: 725 205 1191 210 3722
Fragments arranged by size:
3722 1191 725 210 205
SbfI CC_TGCA'GG
Cuts at: 3100 3100
Size: 6053
ScaI AGT'ACT
Cuts at: 5570 5570
Size: 6053
SexAI A'CCwGG_T
Cuts at: 1837 1837
Size: 6053
SfcI C'TryA_G
Cuts at: 33 718 1381 1745 2383 2515 2645 3096 3781
Size: 685 663 364 638 132 130 451 685
Cuts at: 3781 4774 4965 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 685 663 638 451 364
191 132 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 2434 2434
Size: 6053
SfoI GGC'GCC
Cuts at: 3485 3733 3485
Size: 248 5805
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6053
SmaI CCC'GGG
Cuts at: 163 2848 3075 163
Size: 2685 227 3141
Fragments arranged by size:
3141 2685 227
SmlI C'TyrA_G
Cuts at: 185 706 2230 2371 2929 3208 4123 4615 4877
Size: 521 1524 141 558 279 915 492 262
Cuts at: 4877 185
Size: 1361
Fragments arranged by size:
1524 1361 915 558 521 492 279 262
141
SnaBI TAC'GTA
Cuts at: 3045 3045
Size: 6053
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6053
SphI G_CATG'C
Cuts at: 1546 2728 3106 3606 1546
Size: 1182 378 500 3993
Fragments arranged by size:
3993 1182 500 378
StuI AGG'CCT
Cuts at: 2245 2245
Size: 6053
StyI C'CwwG_G
Cuts at: 125 2702 2868 3187 3541 125
Size: 2577 166 319 354 2637
Fragments arranged by size:
2637 2577 354 319 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1282 1648 3636 3769 4228 4437 1282
Size: 366 1988 133 459 209 2898
Fragments arranged by size:
2898 1988 459 366 209 133
TatI w'GTAC_w
Cuts at: 929 5568 929
Size: 4639 1414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2750 2782 5570 5602 2750
Size: 32 2788 32 3201
Fragments arranged by size:
3201 2788 32 32
Tth111I GACn'n_nGTC
Cuts at: 3850 3850
Size: 6053
XbaI T'CTAG_A
Cuts at: 3084 3084
Size: 6053
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 968 2335 3548 968
Size: 1367 1213 3473
Fragments arranged by size:
3473 1367 1213
XhoI C'TCGA_G
Cuts at: 706 2371 706
Size: 1665 4388
XmaI C'CCGG_G
Cuts at: 161 2846 3073 161
Size: 2685 227 3141
Fragments arranged by size:
3141 2685 227
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmtI BsaXI BsiWI BsrGI BssHII ClaI
CspCI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
SacII SanDI SgrAI SrfI SspI SwaI XmnI ZraI