Restriction map for
pF1KSDA1754
(Circular) (Six-base) MAPSORT of: pF1KSDA1754.seq Check: 2597 from: 1 to: 4778
pF1KSDA1754 4778 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 327 717 327
Size: 390 4388
AccI GT'mk_AC
Cuts at: 1816 1816
Size: 4778
Acc65I G'GTAC_C
Cuts at: 1794 1794
Size: 4778
AclI AA'CG_TT
Cuts at: 4618 4618
Size: 4778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1444 2603 3035 3782 4050 1147
Size: 297 1159 432 747 268 1875
Fragments arranged by size:
1875 1159 747 432 297 268
AfeI AGC'GCT
Cuts at: 568 1712 568
Size: 1144 3634
AflIII A'CryG_T
Cuts at: 372 1734 2130 3234 4211 372
Size: 1362 396 1104 977 939
Fragments arranged by size:
1362 1104 977 939 396
AgeI A'CCGG_T
Cuts at: 1393 3166 1393
Size: 1773 3005
AleI CACnn'nnGTG
Cuts at: 465 744 465
Size: 279 4499
AlwNI CAG_nnn'CTG
Cuts at: 1058 2083 3650 1058
Size: 1025 1567 2186
Fragments arranged by size:
2186 1567 1025
ApaI G_GGCC'C
Cuts at: 458 458
Size: 4778
ApaLI G'TGCA_C
Cuts at: 3548 3548
Size: 4778
ApoI r'AATT_y
Cuts at: 1782 4131 4387 1782
Size: 2349 256 2173
Fragments arranged by size:
2349 2173 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4778
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
AvaI C'yCGr_G
Cuts at: 217 605 775 910 1116 1324 1798 217
Size: 388 170 135 206 208 474 3197
Fragments arranged by size:
3197 474 388 208 206 170 135
BamHI G'GATC_C
Cuts at: 1803 1803
Size: 4778
BanI G'GyrC_C
Cuts at: 508 954 1240 1330 1575 1794 2208 2456 2491
Size: 446 286 90 245 219 414 248 35
Cuts at: 2491 508
Size: 2795
Fragments arranged by size:
2795 446 414 286 248 245 219 90
35
BanII G_rGCy'C
Cuts at: 140 458 782 803 1232 1409 1622 1792 2822
Size: 318 324 21 429 177 213 170 1030
Cuts at: 2822 140
Size: 2096
Fragments arranged by size:
2096 1030 429 324 318 213 177 170
21
BbeI G_GCGC'C
Cuts at: 512 958 1334 2212 2460 512
Size: 446 376 878 248 2830
Fragments arranged by size:
2830 878 446 376 248
BbsI GAAGACnn'nnnn_
Cuts at: 923 1432 923
Size: 509 4269
BbvCI CC'TCA_GC
Cuts at: 1342 1342
Size: 4778
BciVI GTATCCnnnnn_n'
Cuts at: 2676 3437 4778 2676
Size: 761 1341 2676
Fragments arranged by size:
2676 1341 761
BclI T'GATC_A
Cuts at: 2298 2298
Size: 4778
BglII A'GATC_T
Cuts at: 2293 4330 2293
Size: 2037 2741
BlpI GC'TnA_GC
Cuts at: 1408 1890 1408
Size: 482 4296
Bme1580I G_kGCm'C
Cuts at: 458 764 827 1243 2403 2496 3552 458
Size: 306 63 416 1160 93 1056 1684
Fragments arranged by size:
1684 1160 1056 416 306 93 63
BmrI ACTGGGnnnn_n'
Cuts at: 1990 2071 2406 4550 1990
Size: 81 335 2144 2218
Fragments arranged by size:
2218 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 474 3145 474
Size: 2671 2107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 310 352 411 1236 1519 1540 295
Size: 15 42 59 825 283 21 3533
Fragments arranged by size:
3533 825 283 59 42 21 15
Bpu10I CC'TnA_GC
Cuts at: 1342 1609 1342
Size: 267 4511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 1954 2833 3325 3623 1681
Size: 273 879 492 298 2836
Fragments arranged by size:
2836 879 492 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 4482 4482
Size: 4778
BsaAI yAC'GTr
Cuts at: 1770 2131 1770
Size: 361 4417
BsaBI GATnn'nnATC
Cuts at: 6 2292 4329 6
Size: 2286 2037 455
Fragments arranged by size:
2286 2037 455
BsaHI Gr'CG_yC
Cuts at: 509 955 1331 2209 2457 3159 4655 509
Size: 446 376 878 248 702 1496 632
Fragments arranged by size:
1496 878 702 632 446 376 248
BsaWI w'CCGG_w
Cuts at: 1393 1967 2488 3166 3440 3587 3998 1393
Size: 574 521 678 274 147 411 2173
Fragments arranged by size:
2173 678 574 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1195 1225 1427 1457 1195
Size: 30 202 30 4516
Fragments arranged by size:
4516 202 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 244 307 310 361 496 666 1441 241
Size: 3 63 3 51 135 170 775 3578
Fragments arranged by size:
3578 775 170 135 63 51 3 3
BseYI C'CCAG_C
Cuts at: 501 1245 1399 1707 2203 2961 3538 4034 501
Size: 744 154 308 496 758 577 496 1245
Fragments arranged by size:
1245 758 744 577 496 496 308 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1423 1483 919
Size: 504 60 4214
Fragments arranged by size:
4214 504 60
BsiEI CG_ry'CG
Cuts at: 104 317 590 816 2320 2366 3574 104
Size: 213 273 226 1504 46 1208 1308
Fragments arranged by size:
1504 1308 1208 273 226 213 46
BsiHKAI G_wGCw'C
Cuts at: 397 803 811 1610 1792 2570 2760 3552 397
Size: 406 8 799 182 778 190 792 1623
Fragments arranged by size:
1623 799 792 778 406 190 182 8
BsmI GAATG_Cn'
Cuts at: 1707 1707
Size: 4778
Bsp1286I G_dGCh'C
Cuts at: 140 397 458 764 782 803 811 827 1232
Size: 257 61 306 18 21 8 16 405
Cuts at: 1232 1243 1409 1610 1622 1792 2403 2496 2570
Size: 11 166 201 12 170 611 93 74
Cuts at: 2570 2760 2822 3552 140
Size: 190 62 730 1366
Fragments arranged by size:
1366 730 611 405 306 257 201 190
170 166 93 74 62 61 21 18
16 12 11 8
BspEI T'CCGG_A
Cuts at: 3998 3998
Size: 4778
BspHI T'CATG_A
Cuts at: 1025 3954 1025
Size: 2929 1849
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 717 989 1011 1828 2042 2344 2725 327
Size: 390 272 22 817 214 302 381 2380
Fragments arranged by size:
2380 817 390 381 302 272 214 22
BsrBI CCG'CTC
Cuts at: 50 818 3070 3124 4608 4773 50
Size: 768 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 768 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2690 2690
Size: 4778
BsrFI r'CCGG_y
Cuts at: 1393 2776 2957 3166 1393
Size: 1383 181 209 3005
Fragments arranged by size:
3005 1383 209 181
BssSI C'ACGA_G
Cuts at: 1501 3049 3407 1501
Size: 1548 358 2872
Fragments arranged by size:
2872 1548 358
BstAPI GCAn_nnn'nTGC
Cuts at: 401 1506 2155 401
Size: 1105 649 3024
Fragments arranged by size:
3024 1105 649
BstBI TT'CG_AA
Cuts at: 110 3139 110
Size: 3029 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 803 132
Size: 671 4107
BstYI r'GATC_y
Cuts at: 1 1803 2270 2293 2628 2874 3886 3972 3984
Size: 1802 467 23 335 246 1012 86 12
Cuts at: 3984 4330 1
Size: 346 449
Fragments arranged by size:
1802 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 649 1300 1580 1598 649
Size: 651 280 18 3829
Fragments arranged by size:
3829 651 280 18
BtgI C'CryG_G
Cuts at: 125 131 196 584 1586 125
Size: 6 65 388 1002 3317
Fragments arranged by size:
3317 1002 388 65 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1053 2728 2909 3072 1053
Size: 1675 181 163 2759
Fragments arranged by size:
2759 1675 181 163
BtsI GCAGTG_nn'
Cuts at: 739 1318 2021 739
Size: 579 703 3496
Fragments arranged by size:
3496 703 579
DraI TTT'AAA
Cuts at: 1777 1777
Size: 4778
DrdI GACnn_nn'nnGTC
Cuts at: 2484 3342 2484
Size: 858 3920
EaeI y'GGCC_r
Cuts at: 128 167 314 552 587 813 846 1101 2363
Size: 39 147 238 35 226 33 255 1262
Cuts at: 2363 2537 2928 2955 4300 128
Size: 174 391 27 1345 606
Fragments arranged by size:
1345 1262 606 391 255 238 226 174
147 39 35 33 27
EagI C'GGCC_G
Cuts at: 314 587 813 2363 314
Size: 273 226 1550 2729
Fragments arranged by size:
2729 1550 273 226
EarI CTCTTCn'nnn_
Cuts at: 145 418 1519 1636 2801 3011 145
Size: 273 1101 117 1165 210 1912
Fragments arranged by size:
1912 1165 1101 273 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 358 2986 3296 3442 4588 358
Size: 2628 310 146 1146 548
Fragments arranged by size:
2628 1146 548 310 146
EcoICRI GAG'CTC
Cuts at: 801 1790 801
Size: 989 3789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 310 352 411 1147 1236 1444 1519 1540
Size: 15 42 59 736 89 208 75 21
Cuts at: 1540 2603 3035 3782 4050 295
Size: 1063 432 747 268 1023
Fragments arranged by size:
1063 1023 747 736 432 268 208 89
75 59 42 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 292 1456 292
Size: 1164 3614
EcoO109I rG'GnC_Cy
Cuts at: 384 454 455 522 1121 1917 384
Size: 70 1 67 599 796 3245
Fragments arranged by size:
3245 796 599 70 67 1
EcoRI G'AATT_C
Cuts at: 1782 1782
Size: 4778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 4778
FspI TGC'GCA
Cuts at: 2559 2559
Size: 4778
HaeII r_GCGC'y
Cuts at: 512 570 958 1334 1714 2212 2460 3482 4461
Size: 58 388 376 380 498 248 1022 979
Cuts at: 4461 512
Size: 829
Fragments arranged by size:
1022 979 829 498 388 380 376 248
58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1283 1315 2620 2650 2652 2682 1283
Size: 32 1305 30 2 30 3379
Fragments arranged by size:
3379 1305 32 30 30 2
HincII GTy'rAC
Cuts at: 1817 1817
Size: 4778
KasI G'GCGC_C
Cuts at: 508 954 1330 2208 2456 508
Size: 446 376 878 248 2830
Fragments arranged by size:
2830 878 446 376 248
KpnI G_GTAC'C
Cuts at: 1798 1798
Size: 4778
MluI A'CGCG_T
Cuts at: 372 4211 372
Size: 3839 939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3449 3633 3449
Size: 184 4594
MscI TGG'CCA
Cuts at: 130 554 1103 2539 130
Size: 424 549 1436 2369
Fragments arranged by size:
2369 1436 549 424
MslI CAynn'nnrTG
Cuts at: 130 465 560 676 682 700 744 2767 2894
Size: 335 95 116 6 18 44 2023 127
Cuts at: 2894 130
Size: 2014
Fragments arranged by size:
2023 2014 335 127 116 95 44 18
6
MspA1I CmG'CkG
Cuts at: 186 198 273 1107 1346 1403 1485 1889 2203
Size: 12 75 834 239 57 82 404 314
Cuts at: 2203 2563 3576 3821 186
Size: 360 1013 245 1143
Fragments arranged by size:
1143 1013 834 404 360 314 245 239
82 75 57 12
NaeI GCC'GGC
Cuts at: 2959 2959
Size: 4778
NarI GG'CG_CC
Cuts at: 509 955 1331 2209 2457 509
Size: 446 376 878 248 2830
Fragments arranged by size:
2830 878 446 376 248
NcoI C'CATG_G
Cuts at: 125 131 1586 125
Size: 6 1455 3317
Fragments arranged by size:
3317 1455 6
NgoMIV G'CCGG_C
Cuts at: 2957 2957
Size: 4778
NspI r_CATG'y
Cuts at: 714 924 1738 1831 2331 3238 714
Size: 210 814 93 500 907 2254
Fragments arranged by size:
2254 907 814 500 210 93
PasI CC'CwG_GG
Cuts at: 1223 1223
Size: 4778
PciI A'CATG_T
Cuts at: 1734 3234 1734
Size: 1500 3278
PflMI CCAn_nnn'nTGG
Cuts at: 1058 1058
Size: 4778
PfoI T'CCnGG_A
Cuts at: 614 1517 614
Size: 903 3875
PmeI GTTT'AAAC
Cuts at: 1777 1777
Size: 4778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 3917
Size: 32 4746
PpuMI rG'GwC_Cy
Cuts at: 384 1121 384
Size: 737 4041
PshAI GACnn'nnGTC
Cuts at: 1493 1493
Size: 4778
PspOMI G'GGCC_C
Cuts at: 454 454
Size: 4778
PstI C_TGCA'G
Cuts at: 272 1355 1652 1825 2510 272
Size: 1083 297 173 685 2540
Fragments arranged by size:
2540 1083 685 297 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4778
PvuII CAG'CTG
Cuts at: 273 1107 1346 1485 2203 2563 273
Size: 834 239 139 718 360 2488
Fragments arranged by size:
2488 834 718 360 239 139
RsrII CG'GwC_CG
Cuts at: 2973 2973
Size: 4778
SacI G_AGCT'C
Cuts at: 803 1792 803
Size: 989 3789
SacII CC_GC'GG
Cuts at: 199 199
Size: 4778
SalI G'TCGA_C
Cuts at: 1815 1815
Size: 4778
SanDI GG'GwC_CC
Cuts at: 1121 1121
Size: 4778
SapI GCTCTTCn'nnn_
Cuts at: 145 1519 2801 3011 145
Size: 1374 1282 210 1912
Fragments arranged by size:
1912 1374 1282 210
SbfI CC_TGCA'GG
Cuts at: 1825 1825
Size: 4778
ScaI AGT'ACT
Cuts at: 4295 4295
Size: 4778
SfcI C'TryA_G
Cuts at: 33 268 1351 1648 1821 2506 3499 3690 33
Size: 235 1083 297 173 685 993 191 1121
Fragments arranged by size:
1121 1083 993 685 297 235 191 173
SfoI GGC'GCC
Cuts at: 510 956 1332 2210 2458 510
Size: 446 376 878 248 2830
Fragments arranged by size:
2830 878 446 376 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
SmaI CCC'GGG
Cuts at: 607 1118 1800 607
Size: 511 682 3585
Fragments arranged by size:
3585 682 511
SmlI C'TyrA_G
Cuts at: 1696 1933 2848 3340 3602 1696
Size: 237 915 492 262 2872
Fragments arranged by size:
2872 915 492 262 237
SnaBI TAC'GTA
Cuts at: 1770 1770
Size: 4778
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4778
SphI G_CATG'C
Cuts at: 1831 2331 1831
Size: 500 4278
StyI C'CwwG_G
Cuts at: 125 131 1586 1912 2266 125
Size: 6 1455 326 354 2637
Fragments arranged by size:
2637 1455 354 326 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 809 2361 2494 2953 3162 809
Size: 1552 133 459 209 2425
Fragments arranged by size:
2425 1552 459 209 133
TatI w'GTAC_w
Cuts at: 1202 4293 1202
Size: 3091 1687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4295 4327 4295
Size: 32 4746
Tth111I GACn'n_nGTC
Cuts at: 2575 2575
Size: 4778
XbaI T'CTAG_A
Cuts at: 1809 1809
Size: 4778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 2273
Size: 4778
XmaI C'CCGG_G
Cuts at: 605 1116 1798 605
Size: 511 682 3585
Fragments arranged by size:
3585 682 511
XmnI GAAnn'nnTTC
Cuts at: 1033 1033
Size: 4778
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PspXI PsrI SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI ZraI