Restriction map for
pF1KSDA1750
(Circular) (Six-base) MAPSORT of: pF1KSDA1750.seq Check: 101 from: 1 to: 4388
pF1KSDA1750 4388 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 2213 2645 3392 3660 775
Size: 1438 432 747 268 1503
Fragments arranged by size:
1503 1438 747 432 268
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 484 1693 3260 484
Size: 1209 1567 1612
Fragments arranged by size:
1612 1567 1209
ApaI G_GGCC'C
Cuts at: 399 710 399
Size: 311 4077
ApaLI G'TGCA_C
Cuts at: 3158 3158
Size: 4388
ApoI r'AATT_y
Cuts at: 1392 3741 3997 1392
Size: 2349 256 1783
Fragments arranged by size:
2349 1783 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 194 256 343 398 441 870 1408 194
Size: 62 87 55 43 429 538 3174
Fragments arranged by size:
3174 538 429 87 62 55 43
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 1404 1818 2066 2101 1404
Size: 414 248 35 3691
Fragments arranged by size:
3691 414 248 35
BanII G_rGCy'C
Cuts at: 399 710 980 1287 1402 2432 399
Size: 311 270 307 115 1030 2355
Fragments arranged by size:
2355 1030 311 307 270 115
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 269 480 269
Size: 211 4177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 443 477 443
Size: 34 4354
BciVI GTATCCnnnnn_n'
Cuts at: 726 2286 3047 4388 726
Size: 1560 761 1341 726
Fragments arranged by size:
1560 1341 761 726
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 399 710 2013 2106 3162 399
Size: 311 1303 93 1056 1625
Fragments arranged by size:
1625 1303 1056 311 93
BmrI ACTGGGnnnn_n'
Cuts at: 1600 1681 2016 4160 1600
Size: 81 335 2144 1828
Fragments arranged by size:
2144 1828 335 81
BmtI G_CTAG'C
Cuts at: 913 913
Size: 4388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 620 672 2755 620
Size: 52 2083 2253
Fragments arranged by size:
2253 2083 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 760 781 789 1117 194
Size: 566 21 8 328 3465
Fragments arranged by size:
3465 566 328 21 8
Bpu10I CC'TnA_GC
Cuts at: 1141 1141
Size: 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1564 2443 2935 3233 1564
Size: 879 492 298 2719
Fragments arranged by size:
2719 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4092 4092
Size: 4388
BsaAI yAC'GTr
Cuts at: 1380 1741 1380
Size: 361 4027
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 218 1819 2067 2769 4265 218
Size: 1601 248 702 1496 341
Fragments arranged by size:
1601 1496 702 341 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1183 1213 1183
Size: 30 4358
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 364 325
Size: 39 4349
BseYI C'CCAG_C
Cuts at: 667 711 905 1813 2571 3148 3644 667
Size: 44 194 908 758 577 496 1411
Fragments arranged by size:
1411 908 758 577 496 194 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 769 304
Size: 465 3923
BsiEI CG_ry'CG
Cuts at: 104 135 1023 1930 1976 3184 104
Size: 31 888 907 46 1208 1308
Fragments arranged by size:
1308 1208 907 888 46 31
BsiHKAI G_wGCw'C
Cuts at: 980 1052 1402 2180 2370 3162 980
Size: 72 350 778 190 792 2206
Fragments arranged by size:
2206 792 778 350 190 72
Bsp1286I G_dGCh'C
Cuts at: 399 710 980 1052 1287 1402 2013 2106 2180
Size: 311 270 72 235 115 611 93 74
Cuts at: 2180 2370 2432 3162 399
Size: 190 62 730 1625
Fragments arranged by size:
1625 730 611 311 270 235 190 115
93 74 72 62
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1438 1652 1954 2335 1438
Size: 214 302 381 3491
Fragments arranged by size:
3491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 2680 2734 4218 4383 50
Size: 81 2549 54 1484 165 55
Fragments arranged by size:
2549 1484 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 362 545 2386 2567 2776 362
Size: 183 1841 181 209 1974
Fragments arranged by size:
1974 1841 209 183 181
BssSI C'ACGA_G
Cuts at: 1286 2659 3017 1286
Size: 1373 358 2657
Fragments arranged by size:
2657 1373 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1765 1765
Size: 4388
BstBI TT'CG_AA
Cuts at: 110 2749 110
Size: 2639 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1112 1112
Size: 4388
BstYI r'GATC_y
Cuts at: 1 1413 1880 1903 2238 2484 3496 3582 3594
Size: 1412 467 23 335 246 1012 86 12
Cuts at: 3594 3940 1
Size: 346 449
Fragments arranged by size:
1412 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 605 605
Size: 4388
BtgI C'CryG_G
Cuts at: 414 425 509 590 414
Size: 11 84 81 4212
Fragments arranged by size:
4212 84 81 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 1631 1631
Size: 4388
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 132 428 473 1080 1973 2147 2538 2565 3910
Size: 296 45 607 893 174 391 27 1345
Cuts at: 3910 132
Size: 610
Fragments arranged by size:
1345 893 610 607 391 296 174 45
27
EagI C'GGCC_G
Cuts at: 132 1973 132
Size: 1841 2547
EarI CTCTTCn'nnn_
Cuts at: 680 968 2411 2621 680
Size: 288 1443 210 2447
Fragments arranged by size:
2447 1443 288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 796 2596 2906 3052 4198 190
Size: 606 1800 310 146 1146 380
Fragments arranged by size:
1800 1146 606 380 310 146
EcoICRI GAG'CTC
Cuts at: 978 1400 978
Size: 422 3966
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 760 775 781 789 1117 2213 2645 3392
Size: 566 15 6 8 328 1096 432 747
Cuts at: 3392 3660 194
Size: 268 922
Fragments arranged by size:
1096 922 747 566 432 328 268 15
8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1144 1144
Size: 4388
EcoO109I rG'GnC_Cy
Cuts at: 570 601 706 707 788 1527 570
Size: 31 105 1 81 739 3431
Fragments arranged by size:
3431 739 105 81 31 1
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1032 2407 1032
Size: 1375 3013
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 471 1822 2070 3092 4071 471
Size: 1351 248 1022 979 788
Fragments arranged by size:
1351 1022 979 788 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 685 717 1120 1152 2230 2260 2262 2292 685
Size: 32 403 32 1078 30 2 30 2781
Fragments arranged by size:
2781 1078 403 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 519 1397 3059 3243 519
Size: 878 1662 184 1664
Fragments arranged by size:
1664 1662 878 184
MscI TGG'CCA
Cuts at: 1082 2149 1082
Size: 1067 3321
MslI CAynn'nnrTG
Cuts at: 2377 2504 2377
Size: 127 4261
MspA1I CmG'CkG
Cuts at: 319 427 502 592 667 753 1499 1813 2173
Size: 108 75 90 75 86 746 314 360
Cuts at: 2173 3186 3431 319
Size: 1013 245 1276
Fragments arranged by size:
1276 1013 746 360 314 245 108 90
86 75 75
NaeI GCC'GGC
Cuts at: 364 2569 364
Size: 2205 2183
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NgoMIV G'CCGG_C
Cuts at: 362 2567 362
Size: 2205 2183
NheI G'CTAG_C
Cuts at: 909 909
Size: 4388
NspI r_CATG'y
Cuts at: 1441 1941 2848 1441
Size: 500 907 2981
Fragments arranged by size:
2981 907 500
PasI CC'CwG_GG
Cuts at: 1120 1120
Size: 4388
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PfoI T'CCnGG_A
Cuts at: 805 1326 805
Size: 521 3867
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PspOMI G'GGCC_C
Cuts at: 395 706 395
Size: 311 4077
PstI C_TGCA'G
Cuts at: 833 1435 2120 833
Size: 602 685 3101
Fragments arranged by size:
3101 685 602
PvuI CG_AT'CG
Cuts at: 104 1023 104
Size: 919 3469
PvuII CAG'CTG
Cuts at: 667 753 1813 2173 667
Size: 86 1060 360 2882
Fragments arranged by size:
2882 1060 360 86
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 980 1402 980
Size: 422 3966
SacII CC_GC'GG
Cuts at: 428 593 428
Size: 165 4223
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 968 2411 2621 968
Size: 1443 210 2735
Fragments arranged by size:
2735 1443 210
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 582 829 1431 2116 3109 3300 33
Size: 549 247 602 685 993 191 1121
Fragments arranged by size:
1121 993 685 602 549 247 191
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 443 872 1410 443
Size: 429 538 3421
Fragments arranged by size:
3421 538 429
SmlI C'TyrA_G
Cuts at: 1543 2458 2950 3212 1543
Size: 915 492 262 2719
Fragments arranged by size:
2719 915 492 262
SnaBI TAC'GTA
Cuts at: 1380 1380
Size: 4388
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
StyI C'CwwG_G
Cuts at: 1522 1876 1522
Size: 354 4034
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 1055 1169 1971 2104 2563 2772 283
Size: 772 114 802 133 459 209 1899
Fragments arranged by size:
1899 802 772 459 209 133 114
TatI w'GTAC_w
Cuts at: 3903 3903
Size: 4388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1051 1083 3905 3937 1051
Size: 32 2822 32 1502
Fragments arranged by size:
2822 1502 32 32
Tth111I GACn'n_nGTC
Cuts at: 484 2185 484
Size: 1701 2687
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XmaI C'CCGG_G
Cuts at: 441 870 1408 441
Size: 429 538 3421
Fragments arranged by size:
3421 538 429
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PasI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI