Restriction map for
pF1KSDA1748
(Circular) (Six-base) MAPSORT of: pF1KSDA1748.seq Check: 6887 from: 1 to: 5282
pF1KSDA1748 5282 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 267 457 267
Size: 190 5092
AccI GT'mk_AC
Cuts at: 2320 2320
Size: 5282
Acc65I G'GTAC_C
Cuts at: 2248 2298 2248
Size: 50 5232
AclI AA'CG_TT
Cuts at: 5122 5122
Size: 5282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 1538 2143 3107 3539 4286 4554 294
Size: 1244 605 964 432 747 268 1022
Fragments arranged by size:
1244 1022 964 747 605 432 268
AflIII A'CryG_T
Cuts at: 229 2634 3738 4715 229
Size: 2405 1104 977 796
Fragments arranged by size:
2405 1104 977 796
AgeI A'CCGG_T
Cuts at: 3670 3670
Size: 5282
AhdI GACnn_n'nnGTC
Cuts at: 1731 1731
Size: 5282
AleI CACnn'nnGTG
Cuts at: 485 485
Size: 5282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 5282
AlwNI CAG_nnn'CTG
Cuts at: 139 491 1054 1591 2587 4154 139
Size: 352 563 537 996 1567 1267
Fragments arranged by size:
1567 1267 996 563 537 352
ApaLI G'TGCA_C
Cuts at: 4052 4052
Size: 5282
ApoI r'AATT_y
Cuts at: 511 796 2286 4635 4891 511
Size: 285 1490 2349 256 902
Fragments arranged by size:
2349 1490 902 285 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
AvaI C'yCGr_G
Cuts at: 347 1797 1854 2302 347
Size: 1450 57 448 3327
Fragments arranged by size:
3327 1450 448 57
BamHI G'GATC_C
Cuts at: 2307 2307
Size: 5282
BanI G'GyrC_C
Cuts at: 1474 1667 1680 2248 2298 2712 2960 2995 1474
Size: 193 13 568 50 414 248 35 3761
Fragments arranged by size:
3761 568 414 248 193 50 35 13
BanII G_rGCy'C
Cuts at: 390 1325 1709 2296 3326 390
Size: 935 384 587 1030 2346
Fragments arranged by size:
2346 1030 935 587 384
BbeI G_GCGC'C
Cuts at: 2716 2964 2716
Size: 248 5034
BbsI GAAGACnn'nnnn_
Cuts at: 910 2131 910
Size: 1221 4061
BbvCI CC'TCA_GC
Cuts at: 353 997 353
Size: 644 4638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1444 1478 1444
Size: 34 5248
BciVI GTATCCnnnnn_n'
Cuts at: 3180 3941 5282 3180
Size: 761 1341 3180
Fragments arranged by size:
3180 1341 761
BclI T'GATC_A
Cuts at: 516 2802 516
Size: 2286 2996
BglI GCCn_nnn'nGGC
Cuts at: 1642 1962 1642
Size: 320 4962
BglII A'GATC_T
Cuts at: 2797 4834 2797
Size: 2037 3245
BlpI GC'TnA_GC
Cuts at: 2169 2394 2169
Size: 225 5057
Bme1580I G_kGCm'C
Cuts at: 269 1432 1477 2907 3000 4056 269
Size: 1163 45 1430 93 1056 1495
Fragments arranged by size:
1495 1430 1163 1056 93 45
BmrI ACTGGGnnnn_n'
Cuts at: 1933 2494 2575 2910 5054 1933
Size: 561 81 335 2144 2161
Fragments arranged by size:
2161 2144 561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 675 1296 3649 675
Size: 621 2353 2308
Fragments arranged by size:
2353 2308 621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 1045 1221 1392 1741 851
Size: 194 176 171 349 4392
Fragments arranged by size:
4392 349 194 176 171
Bpu10I CC'TnA_GC
Cuts at: 353 997 1073 1084 1592 1966 2163 353
Size: 644 76 11 508 374 197 3472
Fragments arranged by size:
3472 644 508 374 197 76 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2458 3337 3829 4127 1399
Size: 1059 879 492 298 2554
Fragments arranged by size:
2554 1059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 180 882 4986 180
Size: 702 4104 476
Fragments arranged by size:
4104 702 476
BsaAI yAC'GTr
Cuts at: 230 747 2274 2635 230
Size: 517 1527 361 2877
Fragments arranged by size:
2877 1527 517 361
BsaBI GATnn'nnATC
Cuts at: 6 2796 4833 6
Size: 2790 2037 455
Fragments arranged by size:
2790 2037 455
BsaHI Gr'CG_yC
Cuts at: 1929 2713 2961 3663 5159 1929
Size: 784 248 702 1496 2052
Fragments arranged by size:
2052 1496 784 702 248
BsaWI w'CCGG_w
Cuts at: 2471 2992 3670 3944 4091 4502 2471
Size: 521 678 274 147 411 3251
Fragments arranged by size:
3251 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1778 1808 1778
Size: 30 5252
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 1014 1058 1395 1872 2234 370
Size: 644 44 337 477 362 3418
Fragments arranged by size:
3418 644 477 362 337 44
BseYI C'CCAG_C
Cuts at: 157 1306 1324 1443 1646 1932 2707 3465 4042
Size: 1149 18 119 203 286 775 758 577
Cuts at: 4042 4538 157
Size: 496 901
Fragments arranged by size:
1149 901 775 758 577 496 286 203
119 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1600 1600
Size: 5282
BsiEI CG_ry'CG
Cuts at: 104 1192 2026 2824 2870 4078 104
Size: 1088 834 798 46 1208 1308
Fragments arranged by size:
1308 1208 1088 834 798 46
BsiHKAI G_wGCw'C
Cuts at: 390 2296 3074 3264 4056 390
Size: 1906 778 190 792 1616
Fragments arranged by size:
1906 1616 792 778 190
BsmI GAATG_Cn'
Cuts at: 1230 1835 1230
Size: 605 4677
Bsp1286I G_dGCh'C
Cuts at: 269 390 1325 1432 1477 1709 2296 2907 3000
Size: 121 935 107 45 232 587 611 93
Cuts at: 3000 3074 3264 3326 4056 269
Size: 74 190 62 730 1495
Fragments arranged by size:
1495 935 730 611 587 232 190 121
107 93 74 62 45
BspEI T'CCGG_A
Cuts at: 4502 4502
Size: 5282
BspHI T'CATG_A
Cuts at: 4458 4458
Size: 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 267 457 2332 2546 2848 3229 267
Size: 190 1875 214 302 381 2320
Fragments arranged by size:
2320 1875 381 302 214 190
BsrBI CCG'CTC
Cuts at: 50 1316 3574 3628 5112 5277 50
Size: 1266 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 1266 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1524 3194 1524
Size: 1670 3612
BsrFI r'CCGG_y
Cuts at: 3280 3461 3670 3280
Size: 181 209 4892
Fragments arranged by size:
4892 209 181
BsrGI T'GTAC_A
Cuts at: 1178 1178
Size: 5282
BssSI C'ACGA_G
Cuts at: 2211 3553 3911 2211
Size: 1342 358 3582
Fragments arranged by size:
3582 1342 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2659 2659
Size: 5282
BstBI TT'CG_AA
Cuts at: 110 3643 110
Size: 3533 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 327 882 327
Size: 555 4727
BstYI r'GATC_y
Cuts at: 1 2307 2774 2797 3132 3378 4390 4476 4488
Size: 2306 467 23 335 246 1012 86 12
Cuts at: 4488 4834 1
Size: 346 449
Fragments arranged by size:
2306 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3232 3413 3576 3232
Size: 181 163 4938
Fragments arranged by size:
4938 181 163
BtsI GCAGTG_nn'
Cuts at: 269 480 492 522 2525 269
Size: 211 12 30 2003 3026
Fragments arranged by size:
3026 2003 211 30 12
ClaI AT'CG_AT
Cuts at: 2026 2026
Size: 5282
DraI TTT'AAA
Cuts at: 2281 2281
Size: 5282
DraIII CAC_nnn'GTG
Cuts at: 1746 1746
Size: 5282
DrdI GACnn_nn'nnGTC
Cuts at: 2988 3846 2988
Size: 858 4424
EaeI y'GGCC_r
Cuts at: 302 317 758 1148 1189 1502 2867 3041 3432
Size: 15 441 390 41 313 1365 174 391
Cuts at: 3432 3459 4804 302
Size: 27 1345 780
Fragments arranged by size:
1365 1345 780 441 391 390 313 174
41 27 15
EagI C'GGCC_G
Cuts at: 1189 2867 1189
Size: 1678 3604
EarI CTCTTCn'nnn_
Cuts at: 220 3305 3515 220
Size: 3085 210 1987
Fragments arranged by size:
3085 1987 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3490 3800 3946 5092 3490
Size: 310 146 1146 3680
Fragments arranged by size:
3680 1146 310 146
EcoICRI GAG'CTC
Cuts at: 388 2294 388
Size: 1906 3376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 851 1045 1221 1392 1538 1741 2143 3107
Size: 557 194 176 171 146 203 402 964
Cuts at: 3107 3539 4286 4554 294
Size: 432 747 268 1022
Fragments arranged by size:
1022 964 747 557 432 402 268 203
194 176 171 146
EcoNI CCTnn'n_nnAGG
Cuts at: 1994 2183 1994
Size: 189 5093
EcoO109I rG'GnC_Cy
Cuts at: 1588 1985 2421 1588
Size: 397 436 4449
Fragments arranged by size:
4449 436 397
EcoRI G'AATT_C
Cuts at: 2286 2286
Size: 5282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1547 3301 1547
Size: 1754 3528
FspI TGC'GCA
Cuts at: 430 3063 430
Size: 2633 2649
FspAI rTGC'GCAy
Cuts at: 430 430
Size: 5282
HaeII r_GCGC'y
Cuts at: 1244 2716 2964 3986 4965 1244
Size: 1472 248 1022 979 1561
Fragments arranged by size:
1561 1472 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 1027 1059 1284 1316 1372 1404 1558
Size: 32 665 32 225 32 56 32 154
Cuts at: 1558 1590 1717 1749 3124 3154 3156 3186 330
Size: 32 127 32 1375 30 2 30 2426
Fragments arranged by size:
2426 1375 665 225 154 127 56 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1844 2321 1844
Size: 477 4805
KasI G'GCGC_C
Cuts at: 2712 2960 2712
Size: 248 5034
KpnI G_GTAC'C
Cuts at: 2252 2302 2252
Size: 50 5232
MfeI C'AATT_G
Cuts at: 908 1283 908
Size: 375 4907
MluI A'CGCG_T
Cuts at: 4715 4715
Size: 5282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3953 4137 2168
Size: 1785 184 3313
Fragments arranged by size:
3313 1785 184
MscI TGG'CCA
Cuts at: 304 319 760 1150 1504 3043 304
Size: 15 441 390 354 1539 2543
Fragments arranged by size:
2543 1539 441 390 354 15
MslI CAynn'nnrTG
Cuts at: 325 424 485 3271 3398 325
Size: 99 61 2786 127 2209
Fragments arranged by size:
2786 2209 127 99 61
MspA1I CmG'CkG
Cuts at: 1219 1936 2121 2393 2707 3067 4080 4325 1219
Size: 717 185 272 314 360 1013 245 2176
Fragments arranged by size:
2176 1013 717 360 314 272 245 185
NaeI GCC'GGC
Cuts at: 3463 3463
Size: 5282
NarI GG'CG_CC
Cuts at: 2713 2961 2713
Size: 248 5034
NgoMIV G'CCGG_C
Cuts at: 3461 3461
Size: 5282
NspI r_CATG'y
Cuts at: 2335 2835 3742 2335
Size: 500 907 3875
Fragments arranged by size:
3875 907 500
PasI CC'CwG_GG
Cuts at: 334 530 1425 334
Size: 196 895 4191
Fragments arranged by size:
4191 895 196
PciI A'CATG_T
Cuts at: 3738 3738
Size: 5282
PflMI CCAn_nnn'nTGG
Cuts at: 606 1746 606
Size: 1140 4142
PfoI T'CCnGG_A
Cuts at: 234 1757 234
Size: 1523 3759
PmeI GTTT'AAAC
Cuts at: 2281 2281
Size: 5282
PmlI CAC'GTG
Cuts at: 747 747
Size: 5282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1332 1364 4421 4453 1332
Size: 32 3057 32 2161
Fragments arranged by size:
3057 2161 32 32
PpuMI rG'GwC_Cy
Cuts at: 1985 1985
Size: 5282
PspXI vC'TCGA_Gb
Cuts at: 347 347
Size: 5282
PstI C_TGCA'G
Cuts at: 183 264 487 862 1050 2329 3014 183
Size: 81 223 375 188 1279 685 2451
Fragments arranged by size:
2451 1279 685 375 223 188 81
PvuI CG_AT'CG
Cuts at: 104 2026 104
Size: 1922 3360
PvuII CAG'CTG
Cuts at: 1219 1936 2121 2707 3067 1219
Size: 717 185 586 360 3434
Fragments arranged by size:
3434 717 586 360 185
RsrII CG'GwC_CG
Cuts at: 3477 3477
Size: 5282
SacI G_AGCT'C
Cuts at: 390 2296 390
Size: 1906 3376
SalI G'TCGA_C
Cuts at: 2319 2319
Size: 5282
SanDI GG'GwC_CC
Cuts at: 1985 1985
Size: 5282
SapI GCTCTTCn'nnn_
Cuts at: 3305 3515 3305
Size: 210 5072
SbfI CC_TGCA'GG
Cuts at: 2329 2329
Size: 5282
ScaI AGT'ACT
Cuts at: 4799 4799
Size: 5282
SexAI A'CCwGG_T
Cuts at: 2055 2055
Size: 5282
SfcI C'TryA_G
Cuts at: 33 179 260 483 722 858 1046 2133 2325
Size: 146 81 223 239 136 188 1087 192
Cuts at: 2325 3010 4003 4194 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1087 993 685 239 223 192 191
188 146 136 81
SfoI GGC'GCC
Cuts at: 2714 2962 2714
Size: 248 5034
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
SmaI CCC'GGG
Cuts at: 1799 2304 1799
Size: 505 4777
SmlI C'TyrA_G
Cuts at: 347 1414 2437 3352 3844 4106 347
Size: 1067 1023 915 492 262 1523
Fragments arranged by size:
1523 1067 1023 915 492 262
SnaBI TAC'GTA
Cuts at: 2274 2274
Size: 5282
SpeI A'CTAG_T
Cuts at: 67 1873 67
Size: 1806 3476
SphI G_CATG'C
Cuts at: 2335 2835 2335
Size: 500 4782
StyI C'CwwG_G
Cuts at: 1151 2416 2770 1151
Size: 1265 354 3663
Fragments arranged by size:
3663 1265 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 522 598 888 1685 2118 2865 2998 3457 3666
Size: 76 290 797 433 747 133 459 209
Cuts at: 3666 522
Size: 2138
Fragments arranged by size:
2138 797 747 459 433 290 209 133
76
TatI w'GTAC_w
Cuts at: 1178 2115 4797 1178
Size: 937 2682 1663
Fragments arranged by size:
2682 1663 937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 731 763 1778 1810 4799 4831 731
Size: 32 1015 32 2989 32 1182
Fragments arranged by size:
2989 1182 1015 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3079 3079
Size: 5282
XbaI T'CTAG_A
Cuts at: 2313 2313
Size: 5282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 195 312 1516 2777 195
Size: 117 1204 1261 2700
Fragments arranged by size:
2700 1261 1204 117
XhoI C'TCGA_G
Cuts at: 347 347
Size: 5282
XmaI C'CCGG_G
Cuts at: 1797 2302 1797
Size: 505 4777
XmnI GAAnn'nnTTC
Cuts at: 817 817
Size: 5282
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I BtgI CspCI EcoRV FseI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspOMI PsrI SacII SfiI SgrAI SrfI SspI StuI
SwaI ZraI