Restriction map for
pF1KSDA1737
(Circular) (Six-base) MAPSORT of: pF1KSDA1737.seq Check: 668 from: 1 to: 4334
pF1KSDA1737 4334 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 823 823
Size: 4334
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 2159 2591 3338 3606 838
Size: 1321 432 747 268 1566
Fragments arranged by size:
1566 1321 747 432 268
AflIII A'CryG_T
Cuts at: 1686 2790 3767 1686
Size: 1104 977 2253
Fragments arranged by size:
2253 1104 977
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AleI CACnn'nnGTG
Cuts at: 554 554
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 1639 3206 1639
Size: 1567 2767
ApaI G_GGCC'C
Cuts at: 1230 1230
Size: 4334
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 597 1338 3687 3943 597
Size: 741 2349 256 988
Fragments arranged by size:
2349 988 741 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 1354 1354
Size: 4334
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 1350 1764 2012 2047 1350
Size: 414 248 35 3637
Fragments arranged by size:
3637 414 248 35
BanII G_rGCy'C
Cuts at: 157 271 286 1230 1348 2378 157
Size: 114 15 944 118 1030 2113
Fragments arranged by size:
2113 1030 944 118 114 15
BbeI G_GCGC'C
Cuts at: 1768 2016 1768
Size: 248 4086
BbsI GAAGACnn'nnnn_
Cuts at: 168 347 710 168
Size: 179 363 3792
Fragments arranged by size:
3792 363 179
BciVI GTATCCnnnnn_n'
Cuts at: 873 2232 2993 4334 873
Size: 1359 761 1341 873
Fragments arranged by size:
1359 1341 873 761
BclI T'GATC_A
Cuts at: 1308 1854 1308
Size: 546 3788
BglI GCCn_nnn'nGGC
Cuts at: 1234 1234
Size: 4334
BglII A'GATC_T
Cuts at: 1849 3886 1849
Size: 2037 2297
BlpI GC'TnA_GC
Cuts at: 316 1446 316
Size: 1130 3204
Bme1580I G_kGCm'C
Cuts at: 821 1230 1959 2052 3108 821
Size: 409 729 93 1056 2047
Fragments arranged by size:
2047 1056 729 409 93
BmrI ACTGGGnnnn_n'
Cuts at: 884 1546 1627 1962 4106 884
Size: 662 81 335 2144 1112
Fragments arranged by size:
2144 1112 662 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 978 980 2701 978
Size: 2 1721 2611
Fragments arranged by size:
2611 1721 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 436 558 1135 351
Size: 85 122 577 3550
Fragments arranged by size:
3550 577 122 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4038 4038
Size: 4334
BsaAI yAC'GTr
Cuts at: 876 1326 1687 876
Size: 450 361 3523
Fragments arranged by size:
3523 450 361
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 766 1765 2013 2715 4211 766
Size: 999 248 702 1496 889
Fragments arranged by size:
1496 999 889 702 248
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 378 408 378
Size: 30 4304
BseYI C'CCAG_C
Cuts at: 446 778 1196 1314 1759 2517 3094 3590 446
Size: 332 418 118 445 758 577 496 1190
Fragments arranged by size:
1190 758 577 496 445 418 332 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 697
Size: 4334
BsiEI CG_ry'CG
Cuts at: 104 1876 1922 3130 104
Size: 1772 46 1208 1308
Fragments arranged by size:
1772 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 271 286 1019 1348 2126 2316 3108 271
Size: 15 733 329 778 190 792 1497
Fragments arranged by size:
1497 792 778 733 329 190 15
BsmI GAATG_Cn'
Cuts at: 381 1300 381
Size: 919 3415
Bsp1286I G_dGCh'C
Cuts at: 157 271 286 821 1019 1230 1348 1959 2052
Size: 114 15 535 198 211 118 611 93
Cuts at: 2052 2126 2316 2378 3108 157
Size: 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 535 211 198 190 118
114 93 74 62 15
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 407 3510 407
Size: 3103 1231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 823 1384 1598 1900 2281 823
Size: 561 214 302 381 2876
Fragments arranged by size:
2876 561 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 858 2332 2513 2722 858
Size: 1474 181 209 2470
Fragments arranged by size:
2470 1474 209 181
BssSI C'ACGA_G
Cuts at: 2605 2963 2605
Size: 358 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 376 816 1711 376
Size: 440 895 2999
Fragments arranged by size:
2999 895 440
BstBI TT'CG_AA
Cuts at: 110 2695 110
Size: 2585 1749
BstYI r'GATC_y
Cuts at: 1 1359 1826 1849 2184 2430 3442 3528 3540
Size: 1358 467 23 335 246 1012 86 12
Cuts at: 3540 3886 1
Size: 346 449
Fragments arranged by size:
1358 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 324 1232 324
Size: 908 3426
BtgI C'CryG_G
Cuts at: 125 398 945 125
Size: 273 547 3514
Fragments arranged by size:
3514 547 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 1293 1577 1293
Size: 284 4050
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 1919 2093 2484 2511 3856 1919
Size: 174 391 27 1345 2397
Fragments arranged by size:
2397 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 857 2357 2567 857
Size: 1500 210 2624
Fragments arranged by size:
2624 1500 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 269 284 1346 269
Size: 15 1062 3257
Fragments arranged by size:
3257 1062 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 436 558 838 1135 2159 2591 3338 3606
Size: 85 122 280 297 1024 432 747 268
Cuts at: 3606 351
Size: 1079
Fragments arranged by size:
1079 1024 747 432 297 280 268 122
85
EcoO109I rG'GnC_Cy
Cuts at: 636 1226 1227 1473 636
Size: 590 1 246 3497
Fragments arranged by size:
3497 590 246 1
EcoRI G'AATT_C
Cuts at: 597 1338 597
Size: 741 3593
EcoRV GAT'ATC
Cuts at: 1038 1038
Size: 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 396 2353 396
Size: 1957 2377
FspI TGC'GCA
Cuts at: 927 2115 927
Size: 1188 3146
HaeII r_GCGC'y
Cuts at: 323 785 1074 1768 2016 3038 4017 323
Size: 462 289 694 248 1022 979 640
Fragments arranged by size:
1022 979 694 640 462 289 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 253 264 285 550 582 2176 2206 2208
Size: 21 11 21 265 32 1594 30 2
Cuts at: 2208 2238 232
Size: 30 2328
Fragments arranged by size:
2328 1594 265 32 30 30 21 21
11 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 1764 2012 1764
Size: 248 4086
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 3005 3189 593
Size: 2412 184 1738
Fragments arranged by size:
2412 1738 184
MscI TGG'CCA
Cuts at: 2095 2095
Size: 4334
MslI CAynn'nnrTG
Cuts at: 193 554 1002 2323 2450 193
Size: 361 448 1321 127 2077
Fragments arranged by size:
2077 1321 448 361 127
MspA1I CmG'CkG
Cuts at: 1053 1200 1445 1759 2119 3132 3377 1053
Size: 147 245 314 360 1013 245 2010
Fragments arranged by size:
2010 1013 360 314 245 245 147
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 1765 2013 1765
Size: 248 4086
NcoI C'CATG_G
Cuts at: 125 398 945 125
Size: 273 547 3514
Fragments arranged by size:
3514 547 273
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NspI r_CATG'y
Cuts at: 315 1387 1887 2794 315
Size: 1072 500 907 1855
Fragments arranged by size:
1855 1072 907 500
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PfoI T'CCnGG_A
Cuts at: 574 574
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PmlI CAC'GTG
Cuts at: 876 876
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PspOMI G'GGCC_C
Cuts at: 1226 1226
Size: 4334
PstI C_TGCA'G
Cuts at: 291 812 1014 1253 1381 2066 291
Size: 521 202 239 128 685 2559
Fragments arranged by size:
2559 685 521 239 202 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 1200 1759 2119 1200
Size: 559 360 3415
Fragments arranged by size:
3415 559 360
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 271 286 1348 271
Size: 15 1062 3257
Fragments arranged by size:
3257 1062 15
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 287 808 1010 1249 1377 2062 3055 3246
Size: 254 521 202 239 128 685 993 191
Cuts at: 3246 33
Size: 1121
Fragments arranged by size:
1121 993 685 521 254 239 202 191
128
SfoI GGC'GCC
Cuts at: 1766 2014 1766
Size: 248 4086
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 1356 1356
Size: 4334
SmlI C'TyrA_G
Cuts at: 1489 2404 2896 3158 1489
Size: 915 492 262 2665
Fragments arranged by size:
2665 915 492 262
SnaBI TAC'GTA
Cuts at: 1326 1326
Size: 4334
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
StyI C'CwwG_G
Cuts at: 125 398 645 734 945 1133 1468 1822 125
Size: 273 247 89 211 188 335 354 2637
Fragments arranged by size:
2637 354 335 273 247 211 188 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 792 1328 1917 2050 2509 2718 792
Size: 536 589 133 459 209 2408
Fragments arranged by size:
2408 589 536 459 209 133
TatI w'GTAC_w
Cuts at: 691 3849 691
Size: 3158 1176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4302
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 873 1829 873
Size: 956 3378
XmaI C'CCGG_G
Cuts at: 1354 1354
Size: 4334
XmnI GAAnn'nnTTC
Cuts at: 379 379
Size: 4334
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BseRI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI