Restriction map for
pF1KSDA1730
(Circular) (Six-base) MAPSORT of: pF1KSDA1730.seq Check: 6830 from: 1 to: 5369
pF1KSDA1730 5369 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1722 2407 1722
Size: 685 4684
Acc65I G'GTAC_C
Cuts at: 2385 2385
Size: 5369
AclI AA'CG_TT
Cuts at: 5209 5209
Size: 5369
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 686 1138 1853 3194 3626 4373 4641 582
Size: 104 452 715 1341 432 747 268 1310
Fragments arranged by size:
1341 1310 747 715 452 432 268 104
AfeI AGC'GCT
Cuts at: 592 592
Size: 5369
AflIII A'CryG_T
Cuts at: 2151 2721 3825 4802 2151
Size: 570 1104 977 2718
Fragments arranged by size:
2718 1104 977 570
AgeI A'CCGG_T
Cuts at: 3757 3757
Size: 5369
AhdI GACnn_n'nnGTC
Cuts at: 1982 1982
Size: 5369
AleI CACnn'nnGTG
Cuts at: 466 466
Size: 5369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 382 1286 1325 382
Size: 904 39 4426
Fragments arranged by size:
4426 904 39
AlwNI CAG_nnn'CTG
Cuts at: 546 813 2674 4241 546
Size: 267 1861 1567 1674
Fragments arranged by size:
1861 1674 1567 267
ApaLI G'TGCA_C
Cuts at: 1682 4139 1682
Size: 2457 2912
ApoI r'AATT_y
Cuts at: 195 555 1048 1208 1251 1647 1669 1998 2373
Size: 360 493 160 43 396 22 329 375
Cuts at: 2373 4722 4978 195
Size: 2349 256 586
Fragments arranged by size:
2349 586 493 396 375 360 329 256
160 43 22
AseI AT'TA_AT
Cuts at: 20 1858 20
Size: 1838 3531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5369
AvaI C'yCGr_G
Cuts at: 130 574 2389 130
Size: 444 1815 3110
Fragments arranged by size:
3110 1815 444
BamHI G'GATC_C
Cuts at: 524 579 1433 2394 524
Size: 55 854 961 3499
Fragments arranged by size:
3499 961 854 55
BanI G'GyrC_C
Cuts at: 2385 2799 3047 3082 2385
Size: 414 248 35 4672
Fragments arranged by size:
4672 414 248 35
BanII G_rGCy'C
Cuts at: 1598 2383 3413 1598
Size: 785 1030 3554
Fragments arranged by size:
3554 1030 785
BbeI G_GCGC'C
Cuts at: 2803 3051 2803
Size: 248 5121
BbsI GAAGACnn'nnnn_
Cuts at: 662 662
Size: 5369
BciVI GTATCCnnnnn_n'
Cuts at: 3267 4028 5369 3267
Size: 761 1341 3267
Fragments arranged by size:
3267 1341 761
BclI T'GATC_A
Cuts at: 2889 2889
Size: 5369
BglII A'GATC_T
Cuts at: 401 2884 4921 401
Size: 2483 2037 849
Fragments arranged by size:
2483 2037 849
BlpI GC'TnA_GC
Cuts at: 2481 2481
Size: 5369
Bme1580I G_kGCm'C
Cuts at: 1686 2994 3087 4143 1686
Size: 1308 93 1056 2912
Fragments arranged by size:
2912 1308 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1238 2581 2662 2997 5141 1238
Size: 1343 81 335 2144 1466
Fragments arranged by size:
2144 1466 1343 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2339 3736 2018
Size: 321 1397 3651
Fragments arranged by size:
3651 1397 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 523 2030 287
Size: 236 1507 3626
Fragments arranged by size:
3626 1507 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 742 2545 3424 3916 4214 235
Size: 507 1803 879 492 298 1390
Fragments arranged by size:
1803 1390 879 507 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5073 5073
Size: 5369
BsaAI yAC'GTr
Cuts at: 960 2361 2722 960
Size: 1401 361 3607
Fragments arranged by size:
3607 1401 361
BsaBI GATnn'nnATC
Cuts at: 6 2883 4920 6
Size: 2877 2037 455
Fragments arranged by size:
2877 2037 455
BsaHI Gr'CG_yC
Cuts at: 163 2800 3048 3750 5246 163
Size: 2637 248 702 1496 286
Fragments arranged by size:
2637 1496 702 286 248
BsaWI w'CCGG_w
Cuts at: 419 1430 2558 3079 3757 4031 4178 4589 419
Size: 1011 1128 521 678 274 147 411 1199
Fragments arranged by size:
1199 1128 1011 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 148
Size: 30 5339
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 172
Size: 5369
BseYI C'CCAG_C
Cuts at: 2794 3552 4129 4625 2794
Size: 758 577 496 3538
Fragments arranged by size:
3538 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1562
Size: 5369
BsiEI CG_ry'CG
Cuts at: 104 2911 2957 4165 104
Size: 2807 46 1208 1308
Fragments arranged by size:
2807 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1598 1686 2383 3161 3351 4143 1598
Size: 88 697 778 190 792 2824
Fragments arranged by size:
2824 792 778 697 190 88
BsmI GAATG_Cn'
Cuts at: 386 477 1138 2019 386
Size: 91 661 881 3736
Fragments arranged by size:
3736 881 661 91
BsmBI CGTCTCn'nnnn_
Cuts at: 170 170
Size: 5369
Bsp1286I G_dGCh'C
Cuts at: 1598 1686 2383 2994 3087 3161 3351 3413 4143
Size: 88 697 611 93 74 190 62 730
Cuts at: 4143 1598
Size: 2824
Fragments arranged by size:
2824 730 697 611 190 93 88 74
62
BspEI T'CCGG_A
Cuts at: 419 4589 419
Size: 4170 1199
BspHI T'CATG_A
Cuts at: 1757 2235 4545 1757
Size: 478 2310 2581
Fragments arranged by size:
2581 2310 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2419 2633 2935 3316 2419
Size: 214 302 381 4472
Fragments arranged by size:
4472 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3661 3715 5199 5364 50
Size: 3611 54 1484 165 55
Fragments arranged by size:
3611 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 676 3281 676
Size: 2605 2764
BsrFI r'CCGG_y
Cuts at: 142 602 3367 3548 3757 142
Size: 460 2765 181 209 1754
Fragments arranged by size:
2765 1754 460 209 181
BssSI C'ACGA_G
Cuts at: 3640 3998 3640
Size: 358 5011
BstAPI GCAn_nnn'nTGC
Cuts at: 2746 2746
Size: 5369
BstBI TT'CG_AA
Cuts at: 110 3730 110
Size: 3620 1749
BstYI r'GATC_y
Cuts at: 1 401 524 579 1305 1433 2394 2861 2884
Size: 400 123 55 726 128 961 467 23
Cuts at: 2884 3219 3465 4477 4563 4575 4921 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 961 726 467 449 400 346 335
246 128 123 86 55 23 12
BtgI C'CryG_G
Cuts at: 152 308 1367 2310 152
Size: 156 1059 943 3211
Fragments arranged by size:
3211 1059 943 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3319 3500 3663 3319
Size: 181 163 5025
Fragments arranged by size:
5025 181 163
BtsI GCAGTG_nn'
Cuts at: 728 1431 1492 2612 728
Size: 703 61 1120 3485
Fragments arranged by size:
3485 1120 703 61
DraI TTT'AAA
Cuts at: 791 1214 2368 791
Size: 423 1154 3792
Fragments arranged by size:
3792 1154 423
DraIII CAC_nnn'GTG
Cuts at: 1905 2304 1905
Size: 399 4970
DrdI GACnn_nn'nnGTC
Cuts at: 3075 3933 3075
Size: 858 4511
EaeI y'GGCC_r
Cuts at: 2038 2273 2954 3128 3519 3546 4891 2038
Size: 235 681 174 391 27 1345 2516
Fragments arranged by size:
2516 1345 681 391 235 174 27
EagI C'GGCC_G
Cuts at: 2954 2954
Size: 5369
EarI CTCTTCn'nnn_
Cuts at: 896 1603 1759 3392 3602 896
Size: 707 156 1633 210 2663
Fragments arranged by size:
2663 1633 707 210 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3577 3887 4033 5179 3577
Size: 310 146 1146 3767
Fragments arranged by size:
3767 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1596 2381 1596
Size: 785 4584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 523 582 686 1138 1853 2030 3194 3626
Size: 236 59 104 452 715 177 1164 432
Cuts at: 3626 4373 4641 287
Size: 747 268 1015
Fragments arranged by size:
1164 1015 747 715 452 432 268 236
177 104 59
EcoO109I rG'GnC_Cy
Cuts at: 2072 2508 2072
Size: 436 4933
EcoRI G'AATT_C
Cuts at: 555 1048 1998 2373 555
Size: 493 950 375 3551
Fragments arranged by size:
3551 950 493 375
EcoRV GAT'ATC
Cuts at: 186 480 855 186
Size: 294 375 4700
Fragments arranged by size:
4700 375 294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3388 3388
Size: 5369
FspI TGC'GCA
Cuts at: 3150 3150
Size: 5369
HaeII r_GCGC'y
Cuts at: 594 1280 2803 3051 4073 5052 594
Size: 686 1523 248 1022 979 911
Fragments arranged by size:
1523 1022 979 911 686 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 1968 2000 3211 3241 3243 3273 148
Size: 32 1788 32 1211 30 2 30 2244
Fragments arranged by size:
2244 1788 1211 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2408 2408
Size: 5369
HindIII A'AGCT_T
Cuts at: 425 1216 1474 1558 1970 425
Size: 791 258 84 412 3824
Fragments arranged by size:
3824 791 412 258 84
KasI G'GCGC_C
Cuts at: 2799 3047 2799
Size: 248 5121
KpnI G_GTAC'C
Cuts at: 2389 2389
Size: 5369
MluI A'CGCG_T
Cuts at: 4802 4802
Size: 5369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1353 1735 2283 4040 4224 1353
Size: 382 548 1757 184 2498
Fragments arranged by size:
2498 1757 548 382 184
MscI TGG'CCA
Cuts at: 2040 2275 3130 2040
Size: 235 855 4279
Fragments arranged by size:
4279 855 235
MslI CAynn'nnrTG
Cuts at: 466 3358 3485 466
Size: 2892 127 2350
Fragments arranged by size:
2892 2350 127
MspA1I CmG'CkG
Cuts at: 1933 2480 2794 3154 4167 4412 1933
Size: 547 314 360 1013 245 2890
Fragments arranged by size:
2890 1013 547 360 314 245
NaeI GCC'GGC
Cuts at: 144 604 3550 144
Size: 460 2946 1963
Fragments arranged by size:
2946 1963 460
NarI GG'CG_CC
Cuts at: 2800 3048 2800
Size: 248 5121
NcoI C'CATG_G
Cuts at: 152 308 1367 2310 152
Size: 156 1059 943 3211
Fragments arranged by size:
3211 1059 943 156
NgoMIV G'CCGG_C
Cuts at: 142 602 3548 142
Size: 460 2946 1963
Fragments arranged by size:
2946 1963 460
NspI r_CATG'y
Cuts at: 1008 1518 2155 2190 2422 2922 3829 1008
Size: 510 637 35 232 500 907 2548
Fragments arranged by size:
2548 907 637 510 500 232 35
PciI A'CATG_T
Cuts at: 2151 3825 2151
Size: 1674 3695
PflMI CCAn_nnn'nTGG
Cuts at: 546 813 2098 546
Size: 267 1285 3817
Fragments arranged by size:
3817 1285 267
PmeI GTTT'AAAC
Cuts at: 2368 2368
Size: 5369
PmlI CAC'GTG
Cuts at: 960 960
Size: 5369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4508 4540 4508
Size: 32 5337
PpuMI rG'GwC_Cy
Cuts at: 2072 2072
Size: 5369
PshAI GACnn'nnGTC
Cuts at: 759 759
Size: 5369
PspXI vC'TCGA_Gb
Cuts at: 574 574
Size: 5369
PstI C_TGCA'G
Cuts at: 260 1317 2161 2416 3101 260
Size: 1057 844 255 685 2528
Fragments arranged by size:
2528 1057 844 685 255
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5369
PvuII CAG'CTG
Cuts at: 1933 2794 3154 1933
Size: 861 360 4148
Fragments arranged by size:
4148 861 360
RsrII CG'GwC_CG
Cuts at: 3564 3564
Size: 5369
SacI G_AGCT'C
Cuts at: 1598 2383 1598
Size: 785 4584
SalI G'TCGA_C
Cuts at: 2406 2406
Size: 5369
SapI GCTCTTCn'nnn_
Cuts at: 896 1603 3392 3602 896
Size: 707 1789 210 2663
Fragments arranged by size:
2663 1789 707 210
SbfI CC_TGCA'GG
Cuts at: 2416 2416
Size: 5369
ScaI AGT'ACT
Cuts at: 4886 4886
Size: 5369
SfcI C'TryA_G
Cuts at: 33 256 1313 1565 2157 2412 3097 4090 4281
Size: 223 1057 252 592 255 685 993 191
Cuts at: 4281 33
Size: 1121
Fragments arranged by size:
1121 1057 993 685 592 255 252 223
191
SfoI GGC'GCC
Cuts at: 2801 3049 2801
Size: 248 5121
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5369
SmaI CCC'GGG
Cuts at: 2391 2391
Size: 5369
SmlI C'TyrA_G
Cuts at: 214 574 757 2524 3439 3931 4193 214
Size: 360 183 1767 915 492 262 1390
Fragments arranged by size:
1767 1390 915 492 360 262 183
SnaBI TAC'GTA
Cuts at: 2361 2361
Size: 5369
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5369
SphI G_CATG'C
Cuts at: 1008 2190 2422 2922 1008
Size: 1182 232 500 3455
Fragments arranged by size:
3455 1182 500 232
StyI C'CwwG_G
Cuts at: 152 308 1367 1740 2310 2503 2857 152
Size: 156 1059 373 570 193 354 2664
Fragments arranged by size:
2664 1059 570 373 354 193 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 767 2952 3085 3544 3753 767
Size: 2185 133 459 209 2383
Fragments arranged by size:
2383 2185 459 209 133
TatI w'GTAC_w
Cuts at: 1836 4884 1836
Size: 3048 2321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4886 4918 4886
Size: 32 5337
Tth111I GACn'n_nGTC
Cuts at: 3166 3166
Size: 5369
XbaI T'CTAG_A
Cuts at: 1767 2400 1767
Size: 633 4736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2864 2864
Size: 5369
XhoI C'TCGA_G
Cuts at: 574 574
Size: 5369
XmaI C'CCGG_G
Cuts at: 2389 2389
Size: 5369
XmnI GAAnn'nnTTC
Cuts at: 427 1376 427
Size: 949 4420
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI ZraI