Restriction map for
pF1KSDA1722
(Circular) (Six-base) MAPSORT of: pF1KSDA1722.seq Check: 2971 from: 1 to: 7634
pF1KSDA1722 7634 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1052 1541 4672 1052
Size: 489 3131 4014
Fragments arranged by size:
4014 3131 489
Acc65I G'GTAC_C
Cuts at: 4650 4650
Size: 7634
AclI AA'CG_TT
Cuts at: 7474 7474
Size: 7634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2293 2521 3633 5459 5891 6638 6906 2293
Size: 228 1112 1826 432 747 268 3021
Fragments arranged by size:
3021 1826 1112 747 432 268 228
AflII C'TTAA_G
Cuts at: 777 2823 4195 777
Size: 2046 1372 4216
Fragments arranged by size:
4216 2046 1372
AflIII A'CryG_T
Cuts at: 4986 6090 7067 4986
Size: 1104 977 5553
Fragments arranged by size:
5553 1104 977
AgeI A'CCGG_T
Cuts at: 6022 6022
Size: 7634
AleI CACnn'nnGTG
Cuts at: 853 853
Size: 7634
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 478 871 3607 478
Size: 393 2736 4505
Fragments arranged by size:
4505 2736 393
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4394 4426 4394
Size: 32 7602
AlwNI CAG_nnn'CTG
Cuts at: 1527 4587 4939 6506 1527
Size: 3060 352 1567 2655
Fragments arranged by size:
3060 2655 1567 352
ApaI G_GGCC'C
Cuts at: 3680 3680
Size: 7634
ApaLI G'TGCA_C
Cuts at: 6404 6404
Size: 7634
ApoI r'AATT_y
Cuts at: 155 418 1249 1597 1887 4213 4638 6987 7243
Size: 263 831 348 290 2326 425 2349 256
Cuts at: 7243 155
Size: 546
Fragments arranged by size:
2349 2326 831 546 425 348 290 263
256
AseI AT'TA_AT
Cuts at: 20 2224 2363 20
Size: 2204 139 5291
Fragments arranged by size:
5291 2204 139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7634
AvaI C'yCGr_G
Cuts at: 258 1952 3038 4654 258
Size: 1694 1086 1616 3238
Fragments arranged by size:
3238 1694 1616 1086
BamHI G'GATC_C
Cuts at: 4659 4659
Size: 7634
BanI G'GyrC_C
Cuts at: 3870 4081 4462 4650 5064 5312 5347 3870
Size: 211 381 188 414 248 35 6157
Fragments arranged by size:
6157 414 381 248 211 188 35
BanII G_rGCy'C
Cuts at: 1980 3680 4457 4648 5678 1980
Size: 1700 777 191 1030 3936
Fragments arranged by size:
3936 1700 1030 777 191
BbeI G_GCGC'C
Cuts at: 4466 5068 5316 4466
Size: 602 248 6784
Fragments arranged by size:
6784 602 248
BbsI GAAGACnn'nnnn_
Cuts at: 3125 3332 3764 4230 3125
Size: 207 432 466 6529
Fragments arranged by size:
6529 466 432 207
BbvCI CC'TCA_GC
Cuts at: 298 810 1393 298
Size: 512 583 6539
Fragments arranged by size:
6539 583 512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 231 265 2977 3011 231
Size: 34 2712 34 4854
Fragments arranged by size:
4854 2712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1341 5532 6293 7634 1341
Size: 4191 761 1341 1341
Fragments arranged by size:
4191 1341 1341 761
BclI T'GATC_A
Cuts at: 429 4023 5154 429
Size: 3594 1131 2909
Fragments arranged by size:
3594 2909 1131
BglI GCCn_nnn'nGGC
Cuts at: 2725 2725
Size: 7634
BglII A'GATC_T
Cuts at: 2921 5149 7186 2921
Size: 2228 2037 3369
Fragments arranged by size:
3369 2228 2037
BlpI GC'TnA_GC
Cuts at: 1655 4746 1655
Size: 3091 4543
Bme1580I G_kGCm'C
Cuts at: 3449 3680 3875 4423 5259 5352 6408 3449
Size: 231 195 548 836 93 1056 4675
Fragments arranged by size:
4675 1056 836 548 231 195 93
BmrI ACTGGGnnnn_n'
Cuts at: 360 3495 4563 4846 4927 5262 7406 360
Size: 3135 1068 283 81 335 2144 588
Fragments arranged by size:
3135 2144 1068 588 335 283 81
BmtI G_CTAG'C
Cuts at: 2303 2444 2303
Size: 141 7493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4329 6001 4329
Size: 1672 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 568 778 1016 1465 1549 1663 3193 3878
Size: 174 210 238 449 84 114 1530 685
Cuts at: 3878 394
Size: 4150
Fragments arranged by size:
4150 1530 685 449 238 210 174 114
84
Bpu10I CC'TnA_GC
Cuts at: 298 810 1207 1393 298
Size: 512 397 186 6539
Fragments arranged by size:
6539 512 397 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 898 1093 2846 2938 4810 5689 6181 6479
Size: 255 195 1753 92 1872 879 492 298
Cuts at: 6479 643
Size: 1798
Fragments arranged by size:
1872 1798 1753 879 492 298 255 195
92
BsaI GGTCTCn'nnnn_
Cuts at: 799 1273 4335 7338 799
Size: 474 3062 3003 1095
Fragments arranged by size:
3062 3003 1095 474
BsaAI yAC'GTr
Cuts at: 2737 4626 4987 2737
Size: 1889 361 5384
Fragments arranged by size:
5384 1889 361
BsaBI GATnn'nnATC
Cuts at: 6 2465 5148 7185 6
Size: 2459 2683 2037 455
Fragments arranged by size:
2683 2459 2037 455
BsaHI Gr'CG_yC
Cuts at: 2083 4364 4463 5065 5313 6015 7511 2083
Size: 2281 99 602 248 702 1496 2206
Fragments arranged by size:
2281 2206 1496 702 602 248 99
BsaWI w'CCGG_w
Cuts at: 1874 3638 3683 4823 5344 6022 6296 6443 6854
Size: 1764 45 1140 521 678 274 147 411
Cuts at: 6854 1874
Size: 2654
Fragments arranged by size:
2654 1764 1140 678 521 411 274 147
45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 308 338 1715 1745 2635 2665 3424
Size: 30 4 30 1377 30 890 30 759
Cuts at: 3424 3454 4168 4198 274
Size: 30 714 30 3710
Fragments arranged by size:
3710 1377 890 759 714 30 30 30
30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2258 2866 3709 3783 2258
Size: 608 843 74 6109
Fragments arranged by size:
6109 843 608 74
BseYI C'CCAG_C
Cuts at: 540 1660 1804 1812 2181 2906 3618 3672 4471
Size: 1120 144 8 369 725 712 54 799
Cuts at: 4471 4598 5059 5817 6394 6890 540
Size: 127 461 758 577 496 1284
Fragments arranged by size:
1284 1120 799 758 725 712 577 496
461 369 144 127 54 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2593 2776 2531
Size: 62 183 7389
Fragments arranged by size:
7389 183 62
BsiEI CG_ry'CG
Cuts at: 104 1867 4173 5176 5222 6430 104
Size: 1763 2306 1003 46 1208 1308
Fragments arranged by size:
2306 1763 1308 1208 1003 46
BsiHKAI G_wGCw'C
Cuts at: 1841 1980 3961 4648 5426 5616 6408 1841
Size: 139 1981 687 778 190 792 3067
Fragments arranged by size:
3067 1981 792 778 687 190 139
Bsp1286I G_dGCh'C
Cuts at: 1841 1980 3449 3680 3875 3961 4423 4457 4648
Size: 139 1469 231 195 86 462 34 191
Cuts at: 4648 5259 5352 5426 5616 5678 6408 1841
Size: 611 93 74 190 62 730 3067
Fragments arranged by size:
3067 1469 730 611 462 231 195 191
190 139 93 86 74 62 34
BspEI T'CCGG_A
Cuts at: 3683 6854 3683
Size: 3171 4463
BspHI T'CATG_A
Cuts at: 4295 6810 4295
Size: 2515 5119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3077 4684 4898 5200 5581 3077
Size: 1607 214 302 381 5130
Fragments arranged by size:
5130 1607 381 302 214
BsrBI CCG'CTC
Cuts at: 50 368 747 3054 5926 5980 7464 7629 50
Size: 318 379 2307 2872 54 1484 165 55
Fragments arranged by size:
2872 2307 1484 379 318 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5546 5546
Size: 7634
BsrFI r'CCGG_y
Cuts at: 2186 2672 5632 5813 6022 2186
Size: 486 2960 181 209 3798
Fragments arranged by size:
3798 2960 486 209 181
BsrGI T'GTAC_A
Cuts at: 1984 3596 1984
Size: 1612 6022
BssSI C'ACGA_G
Cuts at: 5905 6263 5905
Size: 358 7276
BstAPI GCAn_nnn'nTGC
Cuts at: 4185 4587 5011 4185
Size: 402 424 6808
Fragments arranged by size:
6808 424 402
BstBI TT'CG_AA
Cuts at: 110 5995 110
Size: 5885 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 714 3319 132
Size: 582 2605 4447
Fragments arranged by size:
4447 2605 582
BstYI r'GATC_y
Cuts at: 1 817 2499 2921 3288 4659 5126 5149 5484
Size: 816 1682 422 367 1371 467 23 335
Cuts at: 5484 5730 6742 6828 6840 7186 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1682 1371 1012 816 467 449 422 367
346 335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1542 1542
Size: 7634
Bsu36I CC'TnA_GG
Cuts at: 2035 3359 3790 2035
Size: 1324 431 5879
Fragments arranged by size:
5879 1324 431
BtgI C'CryG_G
Cuts at: 1285 2100 3395 3763 4073 4487 1285
Size: 815 1295 368 310 414 4432
Fragments arranged by size:
4432 1295 815 414 368 310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5584 5765 5928 5584
Size: 181 163 7290
Fragments arranged by size:
7290 181 163
BtsI GCAGTG_nn'
Cuts at: 3127 4423 4585 4877 3127
Size: 1296 162 292 5884
Fragments arranged by size:
5884 1296 292 162
DraI TTT'AAA
Cuts at: 1434 1716 2940 4633 1434
Size: 282 1224 1693 4435
Fragments arranged by size:
4435 1693 1224 282
DraIII CAC_nnn'GTG
Cuts at: 405 405
Size: 7634
DrdI GACnn_nn'nnGTC
Cuts at: 3733 4120 5340 6198 3733
Size: 387 1220 858 5169
Fragments arranged by size:
5169 1220 858 387
EaeI y'GGCC_r
Cuts at: 1864 1961 3826 5219 5393 5784 5811 7156 1864
Size: 97 1865 1393 174 391 27 1345 2342
Fragments arranged by size:
2342 1865 1393 1345 391 174 97 27
EagI C'GGCC_G
Cuts at: 1864 5219 1864
Size: 3355 4279
EarI CTCTTCn'nnn_
Cuts at: 1271 1478 2158 3014 4084 5657 5867 1271
Size: 207 680 856 1070 1573 210 3038
Fragments arranged by size:
3038 1573 1070 856 680 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3230 5842 6152 6298 7444 3230
Size: 2612 310 146 1146 3420
Fragments arranged by size:
3420 2612 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1978 4646 1978
Size: 2668 4966
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 568 778 1016 1465 1549 1663 2293 2521
Size: 174 210 238 449 84 114 630 228
Cuts at: 2521 3193 3633 3878 5459 5891 6638 6906 394
Size: 672 440 245 1581 432 747 268 1122
Fragments arranged by size:
1581 1122 747 672 630 449 440 432
268 245 238 228 210 174 114 84
EcoNI CCTnn'n_nnAGG
Cuts at: 4198 4264 4198
Size: 66 7568
EcoO109I rG'GnC_Cy
Cuts at: 3676 4773 3676
Size: 1097 6537
EcoRI G'AATT_C
Cuts at: 155 1249 4638 155
Size: 1094 3389 3151
Fragments arranged by size:
3389 3151 1094
EcoRV GAT'ATC
Cuts at: 3090 3090
Size: 7634
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1963 2433 5653 1963
Size: 470 3220 3944
Fragments arranged by size:
3944 3220 470
FspI TGC'GCA
Cuts at: 5415 5415
Size: 7634
HaeII r_GCGC'y
Cuts at: 2801 4466 5068 5316 6338 7317 2801
Size: 1665 602 248 1022 979 3118
Fragments arranged by size:
3118 1665 1022 979 602 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1836 1868 1962 1994 3038 3070 3175
Size: 32 1530 32 94 32 1044 32 105
Cuts at: 3175 3207 3532 3564 3691 3723 3990 4022 5476
Size: 32 325 32 127 32 267 32 1454
Cuts at: 5476 5506 5508 5538 274
Size: 30 2 30 2370
Fragments arranged by size:
2370 1530 1454 1044 325 267 127 105
94 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2235 2290 4673 2235
Size: 55 2383 5196
Fragments arranged by size:
5196 2383 55
HindIII A'AGCT_T
Cuts at: 496 496
Size: 7634
HpaI GTT'AAC
Cuts at: 2235 2235
Size: 7634
KasI G'GCGC_C
Cuts at: 4462 5064 5312 4462
Size: 602 248 6784
Fragments arranged by size:
6784 602 248
KpnI G_GTAC'C
Cuts at: 4654 4654
Size: 7634
MluI A'CGCG_T
Cuts at: 7067 7067
Size: 7634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3530 6305 6489 3530
Size: 2775 184 4675
Fragments arranged by size:
4675 2775 184
MscI TGG'CCA
Cuts at: 1963 5395 1963
Size: 3432 4202
MslI CAynn'nnrTG
Cuts at: 130 175 712 853 3317 4336 5623 5750 130
Size: 45 537 141 2464 1019 1287 127 2014
Fragments arranged by size:
2464 2014 1287 1019 537 141 127 45
MspA1I CmG'CkG
Cuts at: 508 540 1808 3515 3765 3983 4745 5059 5419
Size: 32 1268 1707 250 218 762 314 360
Cuts at: 5419 6432 6677 508
Size: 1013 245 1465
Fragments arranged by size:
1707 1465 1268 1013 762 360 314 250
245 218 32
NaeI GCC'GGC
Cuts at: 2188 5815 2188
Size: 3627 4007
NarI GG'CG_CC
Cuts at: 4463 5065 5313 4463
Size: 602 248 6784
Fragments arranged by size:
6784 602 248
NcoI C'CATG_G
Cuts at: 1285 3395 1285
Size: 2110 5524
NdeI CA'TA_TG
Cuts at: 2981 2981
Size: 7634
NgoMIV G'CCGG_C
Cuts at: 2186 5813 2186
Size: 3627 4007
NheI G'CTAG_C
Cuts at: 2299 2440 2299
Size: 141 7493
NspI r_CATG'y
Cuts at: 1802 4137 4192 4687 5187 6094 1802
Size: 2335 55 495 500 907 3342
Fragments arranged by size:
3342 2335 907 500 495 55
PasI CC'CwG_GG
Cuts at: 3711 3711
Size: 7634
PciI A'CATG_T
Cuts at: 6090 6090
Size: 7634
PflMI CCAn_nnn'nTGG
Cuts at: 801 1527 4038 801
Size: 726 2511 4397
Fragments arranged by size:
4397 2511 726
PfoI T'CCnGG_A
Cuts at: 1467 2399 2810 1467
Size: 932 411 6291
Fragments arranged by size:
6291 932 411
PmeI GTTT'AAAC
Cuts at: 4633 4633
Size: 7634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6773 6805 6773
Size: 32 7602
PsiI TTA'TAA
Cuts at: 3719 3719
Size: 7634
PspOMI G'GGCC_C
Cuts at: 3676 3676
Size: 7634
PstI C_TGCA'G
Cuts at: 467 1362 3074 4013 4681 5366 467
Size: 895 1712 939 668 685 2735
Fragments arranged by size:
2735 1712 939 895 685 668
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7634
PvuII CAG'CTG
Cuts at: 508 540 1808 5059 5419 508
Size: 32 1268 3251 360 2723
Fragments arranged by size:
3251 2723 1268 360 32
RsrII CG'GwC_CG
Cuts at: 5829 5829
Size: 7634
SacI G_AGCT'C
Cuts at: 1980 4648 1980
Size: 2668 4966
SacII CC_GC'GG
Cuts at: 3766 3766
Size: 7634
SalI G'TCGA_C
Cuts at: 4671 4671
Size: 7634
SapI GCTCTTCn'nnn_
Cuts at: 4084 5657 5867 4084
Size: 1573 210 5851
Fragments arranged by size:
5851 1573 210
SbfI CC_TGCA'GG
Cuts at: 3074 4681 3074
Size: 1607 6027
ScaI AGT'ACT
Cuts at: 7151 7151
Size: 7634
SexAI A'CCwGG_T
Cuts at: 2423 2423
Size: 7634
SfcI C'TryA_G
Cuts at: 33 463 938 1090 1358 2801 2895 3006 3070
Size: 430 475 152 268 1443 94 111 64
Cuts at: 3070 4009 4677 5362 6355 6546 33
Size: 939 668 685 993 191 1121
Fragments arranged by size:
1443 1121 993 939 685 668 475 430
268 191 152 111 94 64
SfoI GGC'GCC
Cuts at: 4464 5066 5314 4464
Size: 602 248 6784
Fragments arranged by size:
6784 602 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7634
SmaI CCC'GGG
Cuts at: 3040 4656 3040
Size: 1616 6018
SmlI C'TyrA_G
Cuts at: 658 777 913 1108 2823 2861 2917 4195 4789
Size: 119 136 195 1715 38 56 1278 594
Cuts at: 4789 5704 6196 6458 658
Size: 915 492 262 1834
Fragments arranged by size:
1834 1715 1278 915 594 492 262 195
136 119 56 38
SnaBI TAC'GTA
Cuts at: 4626 4626
Size: 7634
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7634
SphI G_CATG'C
Cuts at: 4192 4687 5187 4192
Size: 495 500 6639
Fragments arranged by size:
6639 500 495
SspI AAT'ATT
Cuts at: 1621 3777 1621
Size: 2156 5478
StuI AGG'CCT
Cuts at: 1027 2719 3004 1027
Size: 1692 285 5657
Fragments arranged by size:
5657 1692 285
StyI C'CwwG_G
Cuts at: 1285 1477 3306 3395 3415 3462 4768 5122 1285
Size: 192 1829 89 20 47 1306 354 3797
Fragments arranged by size:
3797 1829 1306 354 192 89 47 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 211 1666 1799 2945 3475 4575 5217 5350 5809
Size: 1455 133 1146 530 1100 642 133 459
Cuts at: 5809 6018 211
Size: 209 1827
Fragments arranged by size:
1827 1455 1146 1100 642 530 459 209
133 133
TatI w'GTAC_w
Cuts at: 521 1984 3596 3935 7149 521
Size: 1463 1612 339 3214 1006
Fragments arranged by size:
3214 1612 1463 1006 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 340 7151 7183 308
Size: 32 6811 32 759
Fragments arranged by size:
6811 759 32 32
Tth111I GACn'n_nGTC
Cuts at: 1048 5431 1048
Size: 4383 3251
XbaI T'CTAG_A
Cuts at: 1188 3552 4665 1188
Size: 2364 1113 4157
Fragments arranged by size:
4157 2364 1113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 220 828 3402 3651 5129 220
Size: 608 2574 249 1478 2725
Fragments arranged by size:
2725 2574 1478 608 249
XmaI C'CCGG_G
Cuts at: 3038 4654 3038
Size: 1616 6018
XmnI GAAnn'nnTTC
Cuts at: 1328 1598 2074 1328
Size: 270 476 6888
Fragments arranged by size:
6888 476 270
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AscI AvrII BaeI BfrBI
BmgBI BsiWI BsmI BsmBI BssHII BstEII ClaI CspCI
FseI FspAI MfeI NotI NruI NsiI PacI PmlI
PpuMI PshAI PspXI PsrI SanDI SfiI SgrAI SrfI
SwaI XhoI ZraI