Restriction map for
pF1KSDA1716
(Circular) (Six-base) MAPSORT of: pF1KSDA1716.seq Check: 8774 from: 1 to: 5414
pF1KSDA1716 5414 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2351 2351
Size: 5414
AccI GT'mk_AC
Cuts at: 896 2452 896
Size: 1556 3858
Acc65I G'GTAC_C
Cuts at: 2430 2430
Size: 5414
AclI AA'CG_TT
Cuts at: 5254 5254
Size: 5414
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 3239 3671 4418 4686 2188
Size: 1051 432 747 268 2916
Fragments arranged by size:
2916 1051 747 432 268
AfeI AGC'GCT
Cuts at: 1431 1431
Size: 5414
AflIII A'CryG_T
Cuts at: 2766 3870 4847 2766
Size: 1104 977 3333
Fragments arranged by size:
3333 1104 977
AgeI A'CCGG_T
Cuts at: 3802 3802
Size: 5414
AleI CACnn'nnGTG
Cuts at: 928 928
Size: 5414
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1708 1740 2338 2370 1708
Size: 32 598 32 4752
Fragments arranged by size:
4752 598 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1801 2719 4286 1801
Size: 918 1567 2929
Fragments arranged by size:
2929 1567 918
ApaI G_GGCC'C
Cuts at: 468 468
Size: 5414
ApaLI G'TGCA_C
Cuts at: 4184 4184
Size: 5414
ApoI r'AATT_y
Cuts at: 2418 4767 5023 2418
Size: 2349 256 2809
Fragments arranged by size:
2809 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5414
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5414
AvaI C'yCGr_G
Cuts at: 426 1184 1902 2319 2434 426
Size: 758 718 417 115 3406
Fragments arranged by size:
3406 758 718 417 115
BamHI G'GATC_C
Cuts at: 2439 2439
Size: 5414
BanI G'GyrC_C
Cuts at: 431 1560 1569 1708 2112 2323 2430 2844 3092
Size: 1129 9 139 404 211 107 414 248
Cuts at: 3092 3127 431
Size: 35 2718
Fragments arranged by size:
2718 1129 414 404 248 211 139 107
35 9
BanII G_rGCy'C
Cuts at: 468 1108 1673 1910 2044 2351 2428 3458 468
Size: 640 565 237 134 307 77 1030 2424
Fragments arranged by size:
2424 1030 640 565 307 237 134 77
BbeI G_GCGC'C
Cuts at: 1564 2116 2327 2848 3096 1564
Size: 552 211 521 248 3882
Fragments arranged by size:
3882 552 521 248 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 150 184 1927 1961 150
Size: 34 1743 34 3603
Fragments arranged by size:
3603 1743 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2377 3312 4073 5414 2377
Size: 935 761 1341 2377
Fragments arranged by size:
2377 1341 935 761
BclI T'GATC_A
Cuts at: 2934 2934
Size: 5414
BglI GCCn_nnn'nGGC
Cuts at: 142 463 1253 1582 1798 1972 2147 142
Size: 321 790 329 216 174 175 3409
Fragments arranged by size:
3409 790 329 321 216 175 174
BglII A'GATC_T
Cuts at: 1442 2929 4966 1442
Size: 1487 2037 1890
Fragments arranged by size:
2037 1890 1487
BlpI GC'TnA_GC
Cuts at: 2526 2526
Size: 5414
Bme1580I G_kGCm'C
Cuts at: 468 1711 1935 3039 3132 4188 468
Size: 1243 224 1104 93 1056 1694
Fragments arranged by size:
1694 1243 1104 1056 224 93
BmgBI CAC'GTC
Cuts at: 786 1887 2085 786
Size: 1101 198 4115
Fragments arranged by size:
4115 1101 198
BmrI ACTGGGnnnn_n'
Cuts at: 582 791 1449 1995 2626 2707 3042 5186 582
Size: 209 658 546 631 81 335 2144 810
Fragments arranged by size:
2144 810 658 631 546 335 209 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1756 1779 3781 1756
Size: 23 2002 3389
Fragments arranged by size:
3389 2002 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 415 502 1600 1617 2335 304
Size: 111 87 1098 17 718 3383
Fragments arranged by size:
3383 1098 718 111 87 17
Bpu10I CC'TnA_GC
Cuts at: 1394 1394
Size: 5414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1687 2590 3469 3961 4259 895
Size: 792 903 879 492 298 2050
Fragments arranged by size:
2050 903 879 792 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5118 5118
Size: 5414
BsaAI yAC'GTr
Cuts at: 1536 2406 2767 1536
Size: 870 361 4183
Fragments arranged by size:
4183 870 361
BsaBI GATnn'nnATC
Cuts at: 6 2928 4965 6
Size: 2922 2037 455
Fragments arranged by size:
2922 2037 455
BsaHI Gr'CG_yC
Cuts at: 179 1561 2014 2113 2324 2845 3093 3795 5291
Size: 1382 453 99 211 521 248 702 1496
Cuts at: 5291 179
Size: 302
Fragments arranged by size:
1496 1382 702 521 453 302 248 211
99
BsaWI w'CCGG_w
Cuts at: 2603 3124 3802 4076 4223 4634 2603
Size: 521 678 274 147 411 3383
Fragments arranged by size:
3383 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1329 1359 2069 2099 2338 2368 1329
Size: 30 710 30 239 30 4375
Fragments arranged by size:
4375 710 239 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1843 1843
Size: 5414
BseYI C'CCAG_C
Cuts at: 194 1280 2136 2839 3597 4174 4670 194
Size: 1086 856 703 758 577 496 938
Fragments arranged by size:
1086 938 856 758 703 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1093 1246 1487 1624 1838 1882 2044 2104
Size: 486 153 241 137 214 44 162 60
Cuts at: 2104 2251 607
Size: 147 3770
Fragments arranged by size:
3770 486 241 214 162 153 147 137
60 44
BsiEI CG_ry'CG
Cuts at: 104 2956 3002 4210 104
Size: 2852 46 1208 1308
Fragments arranged by size:
2852 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 518 955 1324 2351 2428 3206 3396 4188 518
Size: 437 369 1027 77 778 190 792 1744
Fragments arranged by size:
1744 1027 792 778 437 369 190 77
Bsp1286I G_dGCh'C
Cuts at: 468 518 955 1108 1324 1673 1711 1910 1935
Size: 50 437 153 216 349 38 199 25
Cuts at: 1935 2044 2351 2428 3039 3132 3206 3396 3458
Size: 109 307 77 611 93 74 190 62
Cuts at: 3458 4188 468
Size: 730 1694
Fragments arranged by size:
1694 730 611 437 349 307 216 199
190 153 109 93 77 74 62 50
38 25
BspEI T'CCGG_A
Cuts at: 4634 4634
Size: 5414
BspHI T'CATG_A
Cuts at: 4590 4590
Size: 5414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2351 2464 2678 2980 3361 2351
Size: 113 214 302 381 4404
Fragments arranged by size:
4404 381 302 214 113
BsrBI CCG'CTC
Cuts at: 50 176 981 1693 2080 3706 3760 5244 5409
Size: 126 805 712 387 1626 54 1484 165
Cuts at: 5409 50
Size: 55
Fragments arranged by size:
1626 1484 805 712 387 165 126 55
54
BsrDI GCAATG_nn'
Cuts at: 3326 3326
Size: 5414
BsrFI r'CCGG_y
Cuts at: 136 434 860 1237 2146 3412 3593 3802 136
Size: 298 426 377 909 1266 181 209 1748
Fragments arranged by size:
1748 1266 909 426 377 298 209 181
BssHII G'CGCG_C
Cuts at: 2272 2272
Size: 5414
BssSI C'ACGA_G
Cuts at: 442 1155 1383 3685 4043 442
Size: 713 228 2302 358 1813
Fragments arranged by size:
2302 1813 713 358 228
BstAPI GCAn_nnn'nTGC
Cuts at: 1224 1319 1930 2031 2791 1224
Size: 95 611 101 760 3847
Fragments arranged by size:
3847 760 611 101 95
BstBI TT'CG_AA
Cuts at: 110 3775 110
Size: 3665 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 804 1595 2136 804
Size: 791 541 4082
Fragments arranged by size:
4082 791 541
BstYI r'GATC_y
Cuts at: 1 554 1442 2439 2906 2929 3264 3510 4522
Size: 553 888 997 467 23 335 246 1012
Cuts at: 4522 4608 4620 4966 1
Size: 86 12 346 449
Fragments arranged by size:
1012 997 888 553 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 926 1565 1968 2342 125
Size: 801 639 403 374 3197
Fragments arranged by size:
3197 801 639 403 374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1068 2208 3364 3545 3708 1068
Size: 1140 1156 181 163 2774
Fragments arranged by size:
2774 1156 1140 181 163
BtsI GCAGTG_nn'
Cuts at: 205 1350 1643 2234 2257 2657 205
Size: 1145 293 591 23 400 2962
Fragments arranged by size:
2962 1145 591 400 293 23
DraI TTT'AAA
Cuts at: 2413 2413
Size: 5414
DraIII CAC_nnn'GTG
Cuts at: 447 447
Size: 5414
DrdI GACnn_nn'nnGTC
Cuts at: 3120 3978 3120
Size: 858 4556
EaeI y'GGCC_r
Cuts at: 1235 1263 1688 2148 2219 2237 2999 3173 3564
Size: 28 425 460 71 18 762 174 391
Cuts at: 3564 3591 4936 1235
Size: 27 1345 1713
Fragments arranged by size:
1713 1345 762 460 425 391 174 71
28 27 18
EagI C'GGCC_G
Cuts at: 2999 2999
Size: 5414
EarI CTCTTCn'nnn_
Cuts at: 829 1747 1774 2390 3437 3647 829
Size: 918 27 616 1047 210 2596
Fragments arranged by size:
2596 1047 918 616 210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 481 934 1080 1161 1717 1897 2005 2296 3622
Size: 453 146 81 556 180 108 291 1326
Cuts at: 3622 3932 4078 5224 481
Size: 310 146 1146 671
Fragments arranged by size:
1326 1146 671 556 453 310 291 180
146 146 108 81
EcoICRI GAG'CTC
Cuts at: 2349 2426 2349
Size: 77 5337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 415 502 1600 1617 2188 2335 3239 3671
Size: 111 87 1098 17 571 147 904 432
Cuts at: 3671 4418 4686 304
Size: 747 268 1032
Fragments arranged by size:
1098 1032 904 747 571 432 268 147
111 87 17
EcoNI CCTnn'n_nnAGG
Cuts at: 256 256
Size: 5414
EcoO109I rG'GnC_Cy
Cuts at: 390 423 464 465 1584 1875 2211 2553 390
Size: 33 41 1 1119 291 336 342 3251
Fragments arranged by size:
3251 1119 342 336 291 41 33 1
EcoRI G'AATT_C
Cuts at: 2418 2418
Size: 5414
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3433 3433
Size: 5414
FspI TGC'GCA
Cuts at: 3195 3195
Size: 5414
HaeII r_GCGC'y
Cuts at: 867 1433 1564 1963 2116 2327 2848 3096 4118
Size: 566 131 399 153 211 521 248 1022
Cuts at: 4118 5097 867
Size: 979 1184
Fragments arranged by size:
1184 1022 979 566 521 399 248 211
153 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 988 1020 3256 3286 3288 3318 988
Size: 32 2236 30 2 30 3084
Fragments arranged by size:
3084 2236 32 30 30 2
HincII GTy'rAC
Cuts at: 1983 2453 1983
Size: 470 4944
KasI G'GCGC_C
Cuts at: 1560 2112 2323 2844 3092 1560
Size: 552 211 521 248 3882
Fragments arranged by size:
3882 552 521 248 211
KpnI G_GTAC'C
Cuts at: 2434 2434
Size: 5414
MluI A'CGCG_T
Cuts at: 4847 4847
Size: 5414
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1805 4085 4269 1805
Size: 2280 184 2950
Fragments arranged by size:
2950 2280 184
MscI TGG'CCA
Cuts at: 2221 3175 2221
Size: 954 4460
MslI CAynn'nnrTG
Cuts at: 928 2227 3403 3530 928
Size: 1299 1176 127 2812
Fragments arranged by size:
2812 1299 1176 127
MspA1I CmG'CkG
Cuts at: 630 672 735 891 1280 1838 2019 2208 2525
Size: 42 63 156 389 558 181 189 317
Cuts at: 2525 2839 3199 4212 4457 630
Size: 314 360 1013 245 1587
Fragments arranged by size:
1587 1013 558 389 360 317 314 245
189 181 156 63 42
NaeI GCC'GGC
Cuts at: 1239 3595 1239
Size: 2356 3058
NarI GG'CG_CC
Cuts at: 1561 2113 2324 2845 3093 1561
Size: 552 211 521 248 3882
Fragments arranged by size:
3882 552 521 248 211
NcoI C'CATG_G
Cuts at: 125 1565 125
Size: 1440 3974
NgoMIV G'CCGG_C
Cuts at: 1237 3593 1237
Size: 2356 3058
NspI r_CATG'y
Cuts at: 1026 2467 2967 3874 1026
Size: 1441 500 907 2566
Fragments arranged by size:
2566 1441 907 500
PciI A'CATG_T
Cuts at: 3870 3870
Size: 5414
PflMI CCAn_nnn'nTGG
Cuts at: 447 447
Size: 5414
PfoI T'CCnGG_A
Cuts at: 557 2369 557
Size: 1812 3602
PmeI GTTT'AAAC
Cuts at: 2413 2413
Size: 5414
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 970 1002 2338 2370 4553 4585 970
Size: 32 1336 32 2183 32 1799
Fragments arranged by size:
2183 1799 1336 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 390 2211 390
Size: 1821 3593
PspOMI G'GGCC_C
Cuts at: 464 464
Size: 5414
PstI C_TGCA'G
Cuts at: 236 536 749 1031 1220 2461 3146 236
Size: 300 213 282 189 1241 685 2504
Fragments arranged by size:
2504 1241 685 300 282 213 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5414
PvuII CAG'CTG
Cuts at: 630 672 891 2839 3199 630
Size: 42 219 1948 360 2845
Fragments arranged by size:
2845 1948 360 219 42
RsrII CG'GwC_CG
Cuts at: 3609 3609
Size: 5414
SacI G_AGCT'C
Cuts at: 2351 2428 2351
Size: 77 5337
SalI G'TCGA_C
Cuts at: 2451 2451
Size: 5414
SanDI GG'GwC_CC
Cuts at: 2211 2211
Size: 5414
SapI GCTCTTCn'nnn_
Cuts at: 1774 3437 3647 1774
Size: 1663 210 3541
Fragments arranged by size:
3541 1663 210
SbfI CC_TGCA'GG
Cuts at: 2461 2461
Size: 5414
ScaI AGT'ACT
Cuts at: 4931 4931
Size: 5414
SfcI C'TryA_G
Cuts at: 33 232 253 532 612 745 1027 1119 1216
Size: 199 21 279 80 133 282 92 97
Cuts at: 1216 2457 3142 4135 4326 33
Size: 1241 685 993 191 1121
Fragments arranged by size:
1241 1121 993 685 282 279 199 191
133 97 92 80 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 1972 2147 1972
Size: 175 5239
SfoI GGC'GCC
Cuts at: 1562 2114 2325 2846 3094 1562
Size: 552 211 521 248 3882
Fragments arranged by size:
3882 552 521 248 211
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5414
SmaI CCC'GGG
Cuts at: 1904 2321 2436 1904
Size: 417 115 4882
Fragments arranged by size:
4882 417 115
SmlI C'TyrA_G
Cuts at: 910 1666 2569 3484 3976 4238 910
Size: 756 903 915 492 262 2086
Fragments arranged by size:
2086 915 903 756 492 262
SnaBI TAC'GTA
Cuts at: 2406 2406
Size: 5414
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5414
SphI G_CATG'C
Cuts at: 1026 2467 2967 1026
Size: 1441 500 3473
Fragments arranged by size:
3473 1441 500
StuI AGG'CCT
Cuts at: 145 1516 1870 145
Size: 1371 354 3689
Fragments arranged by size:
3689 1371 354
StyI C'CwwG_G
Cuts at: 125 1358 1565 2548 2902 125
Size: 1233 207 983 354 2637
Fragments arranged by size:
2637 1233 983 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1023 1333 2997 3130 3589 3798 1023
Size: 310 1664 133 459 209 2639
Fragments arranged by size:
2639 1664 459 310 209 133
TatI w'GTAC_w
Cuts at: 4929 4929
Size: 5414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 574 606 1329 1361 2069 2101 4931 4963 574
Size: 32 723 32 708 32 2830 32 1025
Fragments arranged by size:
2830 1025 723 708 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 214 3211 214
Size: 2997 2417
XbaI T'CTAG_A
Cuts at: 2445 2445
Size: 5414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1293 2909 1293
Size: 1616 3798
XmaI C'CCGG_G
Cuts at: 1902 2319 2434 1902
Size: 417 115 4882
Fragments arranged by size:
4882 417 115
XmnI GAAnn'nnTTC
Cuts at: 487 487
Size: 5414
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AscI AvrII BaeI BbsI
BbvCI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SgrAI SrfI SspI SwaI XhoI ZraI