Restriction map for
pF1KSDA1715
(Circular) (Six-base) MAPSORT of: pF1KSDA1715.seq Check: 2293 from: 1 to: 4421
pF1KSDA1715 4421 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1092 1092
Size: 4421
AccI GT'mk_AC
Cuts at: 1459 1459
Size: 4421
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 2246 2678 3425 3693 800
Size: 1446 432 747 268 1528
Fragments arranged by size:
1528 1446 747 432 268
AflIII A'CryG_T
Cuts at: 1773 2877 3854 1773
Size: 1104 977 2340
Fragments arranged by size:
2340 1104 977
AgeI A'CCGG_T
Cuts at: 2809 2809
Size: 4421
AlwNI CAG_nnn'CTG
Cuts at: 823 1354 1726 3293 823
Size: 531 372 1567 1951
Fragments arranged by size:
1951 1567 531 372
ApaI G_GGCC'C
Cuts at: 685 685
Size: 4421
ApaLI G'TGCA_C
Cuts at: 3191 3191
Size: 4421
ApoI r'AATT_y
Cuts at: 197 214 331 994 1425 3774 4030 197
Size: 17 117 663 431 2349 256 588
Fragments arranged by size:
2349 663 588 431 256 117 17
AseI AT'TA_AT
Cuts at: 20 262 526 20
Size: 242 264 3915
Fragments arranged by size:
3915 264 242
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 875 1441 875
Size: 566 3855
BamHI G'GATC_C
Cuts at: 802 1446 802
Size: 644 3777
BanI G'GyrC_C
Cuts at: 1437 1851 2099 2134 1437
Size: 414 248 35 3724
Fragments arranged by size:
3724 414 248 35
BanII G_rGCy'C
Cuts at: 685 1435 2465 685
Size: 750 1030 2641
Fragments arranged by size:
2641 1030 750
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BbsI GAAGACnn'nnnn_
Cuts at: 826 826
Size: 4421
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3080 4421 2319
Size: 761 1341 2319
Fragments arranged by size:
2319 1341 761
BclI T'GATC_A
Cuts at: 380 1941 380
Size: 1561 2860
BglII A'GATC_T
Cuts at: 1936 3973 1936
Size: 2037 2384
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 685 2046 2139 3195 685
Size: 1361 93 1056 1911
Fragments arranged by size:
1911 1361 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1633 1714 2049 4193 1633
Size: 81 335 2144 1861
Fragments arranged by size:
2144 1861 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1341 2788 1341
Size: 1447 2974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 608 749 827 1376 408
Size: 200 141 78 549 3453
Fragments arranged by size:
3453 549 200 141 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2476 2968 3266 1597
Size: 879 492 298 2752
Fragments arranged by size:
2752 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4125 4125
Size: 4421
BsaAI yAC'GTr
Cuts at: 1413 1774 1413
Size: 361 4060
BsaBI GATnn'nnATC
Cuts at: 6 1935 3972 6
Size: 1929 2037 455
Fragments arranged by size:
2037 1929 455
BsaHI Gr'CG_yC
Cuts at: 1852 2100 2802 4298 1852
Size: 248 702 1496 1975
Fragments arranged by size:
1975 1496 702 248
BsaWI w'CCGG_w
Cuts at: 546 1610 2131 2809 3083 3230 3641 546
Size: 1064 521 678 274 147 411 1326
Fragments arranged by size:
1326 1064 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 581 611 747 777 854 884 581
Size: 30 136 30 77 30 4118
Fragments arranged by size:
4118 136 77 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 677 1309 677
Size: 632 3789
BseYI C'CCAG_C
Cuts at: 1846 2604 3181 3677 1846
Size: 758 577 496 2590
Fragments arranged by size:
2590 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1963 2009 3217 104
Size: 1859 46 1208 1308
Fragments arranged by size:
1859 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1435 2213 2403 3195 1435
Size: 778 190 792 2661
Fragments arranged by size:
2661 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 685 1435 2046 2139 2213 2403 2465 3195 685
Size: 750 611 93 74 190 62 730 1911
Fragments arranged by size:
1911 750 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 546 3641 546
Size: 3095 1326
BspHI T'CATG_A
Cuts at: 3597 3597
Size: 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1092 1471 1685 1987 2368 1092
Size: 379 214 302 381 3145
Fragments arranged by size:
3145 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 2713 2767 4251 4416 50
Size: 2663 54 1484 165 55
Fragments arranged by size:
2663 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1004 2333 1004
Size: 1329 3092
BsrFI r'CCGG_y
Cuts at: 2419 2600 2809 2419
Size: 181 209 4031
Fragments arranged by size:
4031 209 181
BssSI C'ACGA_G
Cuts at: 2692 3050 2692
Size: 358 4063
BstAPI GCAn_nnn'nTGC
Cuts at: 346 1798 346
Size: 1452 2969
BstBI TT'CG_AA
Cuts at: 110 2782 110
Size: 2672 1749
BstYI r'GATC_y
Cuts at: 1 802 1446 1913 1936 2271 2517 3529 3615
Size: 801 644 467 23 335 246 1012 86
Cuts at: 3615 3627 3973 1
Size: 12 346 449
Fragments arranged by size:
1012 801 644 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1061 1061
Size: 4421
BtgI C'CryG_G
Cuts at: 125 125
Size: 4421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2371 2552 2715 2371
Size: 181 163 4077
Fragments arranged by size:
4077 181 163
BtsI GCAGTG_nn'
Cuts at: 964 1664 964
Size: 700 3721
DraI TTT'AAA
Cuts at: 461 1420 461
Size: 959 3462
DrdI GACnn_nn'nnGTC
Cuts at: 2127 2985 2127
Size: 858 3563
EaeI y'GGCC_r
Cuts at: 2006 2180 2571 2598 3943 2006
Size: 174 391 27 1345 2484
Fragments arranged by size:
2484 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 1090 2444 2654 1090
Size: 1354 210 2857
Fragments arranged by size:
2857 1354 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2629 2939 3085 4231 2629
Size: 310 146 1146 2819
Fragments arranged by size:
2819 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1433 1433
Size: 4421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 608 749 800 827 1376 2246 2678 3425
Size: 200 141 51 27 549 870 432 747
Cuts at: 3425 3693 408
Size: 268 1136
Fragments arranged by size:
1136 870 747 549 432 268 200 141
51 27
EcoO109I rG'GnC_Cy
Cuts at: 681 682 1560 681
Size: 1 878 3542
Fragments arranged by size:
3542 878 1
EcoRI G'AATT_C
Cuts at: 1425 1425
Size: 4421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4421
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 1855 2103 3125 4104 1855
Size: 248 1022 979 2172
Fragments arranged by size:
2172 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 535 565 567 597 747 779 854
Size: 32 55 30 2 30 150 32 75
Cuts at: 854 886 2263 2293 2295 2325 448
Size: 32 1377 30 2 30 2544
Fragments arranged by size:
2544 1377 150 75 55 32 32 32
30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 1460 1460
Size: 4421
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 234 682 3092 3276 234
Size: 448 2410 184 1379
Fragments arranged by size:
2410 1379 448 184
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 948 2410 2537 948
Size: 1462 127 2832
Fragments arranged by size:
2832 1462 127
MspA1I CmG'CkG
Cuts at: 595 1532 1846 2206 3219 3464 595
Size: 937 314 360 1013 245 1552
Fragments arranged by size:
1552 1013 937 360 314 245
NaeI GCC'GGC
Cuts at: 2602 2602
Size: 4421
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NcoI C'CATG_G
Cuts at: 125 125
Size: 4421
NgoMIV G'CCGG_C
Cuts at: 2600 2600
Size: 4421
NspI r_CATG'y
Cuts at: 299 1474 1974 2881 299
Size: 1175 500 907 1839
Fragments arranged by size:
1839 1175 907 500
PciI A'CATG_T
Cuts at: 2877 2877
Size: 4421
PflMI CCAn_nnn'nTGG
Cuts at: 586 586
Size: 4421
PfoI T'CCnGG_A
Cuts at: 708 1353 708
Size: 645 3776
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 624 656 3560 3592 624
Size: 32 2904 32 1453
Fragments arranged by size:
2904 1453 32 32
PspOMI G'GGCC_C
Cuts at: 681 681
Size: 4421
PstI C_TGCA'G
Cuts at: 639 1468 2153 639
Size: 829 685 2907
Fragments arranged by size:
2907 829 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 595 1846 2206 595
Size: 1251 360 2810
Fragments arranged by size:
2810 1251 360
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 1435 1435
Size: 4421
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 3938 3938
Size: 4421
SfcI C'TryA_G
Cuts at: 33 167 635 1241 1464 2149 3142 3333 33
Size: 134 468 606 223 685 993 191 1121
Fragments arranged by size:
1121 993 685 606 468 223 191 134
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 1443 1443
Size: 4421
SmlI C'TyrA_G
Cuts at: 1576 2491 2983 3245 1576
Size: 915 492 262 2752
Fragments arranged by size:
2752 915 492 262
SnaBI TAC'GTA
Cuts at: 1413 1413
Size: 4421
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
SspI AAT'ATT
Cuts at: 910 910
Size: 4421
StyI C'CwwG_G
Cuts at: 125 1555 1909 125
Size: 1430 354 2637
Fragments arranged by size:
2637 1430 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 118 2004 2137 2596 2805 118
Size: 1886 133 459 209 1734
Fragments arranged by size:
1886 1734 459 209 133
TatI w'GTAC_w
Cuts at: 3936 3936
Size: 4421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4389
Tth111I GACn'n_nGTC
Cuts at: 2218 2218
Size: 4421
XbaI T'CTAG_A
Cuts at: 177 1452 177
Size: 1275 3146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 383 1916 383
Size: 1533 2888
XmaI C'CCGG_G
Cuts at: 1441 1441
Size: 4421
XmnI GAAnn'nnTTC
Cuts at: 651 651
Size: 4421
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI