Restriction map for
pF1KSDA1711
(Circular) (Six-base) MAPSORT of: pF1KSDA1711.seq Check: 5710 from: 1 to: 6230
pF1KSDA1711 6230 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 1916 1916
Size: 6230
AccI GT'mk_AC
Cuts at: 956 3268 956
Size: 2312 3918
Acc65I G'GTAC_C
Cuts at: 3246 3246
Size: 6230
AclI AA'CG_TT
Cuts at: 3087 6070 3087
Size: 2983 3247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 359 1433 1691 2762 3134 4055 4487 5234
Size: 232 1074 258 1071 372 921 432 747
Cuts at: 5234 5502 127
Size: 268 855
Fragments arranged by size:
1074 1071 921 855 747 432 372 268
258 232
AflII C'TTAA_G
Cuts at: 3075 3075
Size: 6230
AflIII A'CryG_T
Cuts at: 676 1066 1473 2493 3582 4686 5663 676
Size: 390 407 1020 1089 1104 977 1243
Fragments arranged by size:
1243 1104 1089 1020 977 407 390
AgeI A'CCGG_T
Cuts at: 4618 4618
Size: 6230
AleI CACnn'nnGTG
Cuts at: 884 884
Size: 6230
AlwNI CAG_nnn'CTG
Cuts at: 2431 3007 3535 5102 2431
Size: 576 528 1567 3559
Fragments arranged by size:
3559 1567 576 528
ApaLI G'TGCA_C
Cuts at: 2970 5000 2970
Size: 2030 4200
ApoI r'AATT_y
Cuts at: 219 427 1545 1579 2476 2625 2777 2912 3234
Size: 208 1118 34 897 149 152 135 322
Cuts at: 3234 5583 5839 219
Size: 2349 256 610
Fragments arranged by size:
2349 1118 897 610 322 256 208 152
149 135 34
AseI AT'TA_AT
Cuts at: 20 349 1928 20
Size: 329 1579 4322
Fragments arranged by size:
4322 1579 329
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6230
AvaI C'yCGr_G
Cuts at: 479 3250 479
Size: 2771 3459
AvrII C'CTAG_G
Cuts at: 1312 1312
Size: 6230
BamHI G'GATC_C
Cuts at: 3255 3255
Size: 6230
BanI G'GyrC_C
Cuts at: 309 2043 3246 3660 3908 3943 309
Size: 1734 1203 414 248 35 2596
Fragments arranged by size:
2596 1734 1203 414 248 35
BanII G_rGCy'C
Cuts at: 1488 3244 4274 1488
Size: 1756 1030 3444
Fragments arranged by size:
3444 1756 1030
BbeI G_GCGC'C
Cuts at: 3664 3912 3664
Size: 248 5982
BbsI GAAGACnn'nnnn_
Cuts at: 1211 1457 1679 1211
Size: 246 222 5762
Fragments arranged by size:
5762 246 222
BbvCI CC'TCA_GC
Cuts at: 1946 3192 1946
Size: 1246 4984
BciVI GTATCCnnnnn_n'
Cuts at: 922 1759 4128 4889 6230 922
Size: 837 2369 761 1341 922
Fragments arranged by size:
2369 1341 922 837 761
BclI T'GATC_A
Cuts at: 1029 2376 3750 1029
Size: 1347 1374 3509
Fragments arranged by size:
3509 1374 1347
BfrBI ATG'CAT
Cuts at: 2221 2248 2221
Size: 27 6203
BglI GCCn_nnn'nGGC
Cuts at: 1868 1868
Size: 6230
BglII A'GATC_T
Cuts at: 3745 5782 3745
Size: 2037 4193
BlpI GC'TnA_GC
Cuts at: 3342 3342
Size: 6230
Bme1580I G_kGCm'C
Cuts at: 2974 3116 3855 3948 5004 2974
Size: 142 739 93 1056 4200
Fragments arranged by size:
4200 1056 739 142 93
BmgBI CAC'GTC
Cuts at: 1455 1455
Size: 6230
BmrI ACTGGGnnnn_n'
Cuts at: 1394 3442 3523 3858 6002 1394
Size: 2048 81 335 2144 1622
Fragments arranged by size:
2144 2048 1622 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4597 4597
Size: 6230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 2260
Size: 6230
Bpu10I CC'TnA_GC
Cuts at: 1946 3192 1946
Size: 1246 4984
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 3406 4285 4777 5075 1501
Size: 1905 879 492 298 2656
Fragments arranged by size:
2656 1905 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5934 5934
Size: 6230
BsaAI yAC'GTr
Cuts at: 1069 3222 3583 1069
Size: 2153 361 3716
Fragments arranged by size:
3716 2153 361
BsaBI GATnn'nnATC
Cuts at: 6 2585 3744 5781 6
Size: 2579 1159 2037 455
Fragments arranged by size:
2579 2037 1159 455
BsaHI Gr'CG_yC
Cuts at: 1403 1913 3661 3909 4611 6107 1403
Size: 510 1748 248 702 1496 1526
Fragments arranged by size:
1748 1526 1496 702 510 248
BsaWI w'CCGG_w
Cuts at: 3419 3940 4618 4892 5039 5450 3419
Size: 521 678 274 147 411 4199
Fragments arranged by size:
4199 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1034 1064 2502 2532 1034
Size: 30 1438 30 4732
Fragments arranged by size:
4732 1438 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1288 1291 1309 1312 1863 2835 1288
Size: 3 18 3 551 972 4683
Fragments arranged by size:
4683 972 551 18 3 3
BseYI C'CCAG_C
Cuts at: 3206 3655 4413 4990 5486 3206
Size: 449 758 577 496 3950
Fragments arranged by size:
3950 758 577 496 449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1647 1647
Size: 6230
BsiEI CG_ry'CG
Cuts at: 104 3772 3818 5026 104
Size: 3668 46 1208 1308
Fragments arranged by size:
3668 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1090 1488 2974 3244 4022 4212 5004 1090
Size: 398 1486 270 778 190 792 2316
Fragments arranged by size:
2316 1486 792 778 398 270 190
BsmI GAATG_Cn'
Cuts at: 672 3133 672
Size: 2461 3769
Bsp1286I G_dGCh'C
Cuts at: 1090 1488 2974 3116 3244 3855 3948 4022 4212
Size: 398 1486 142 128 611 93 74 190
Cuts at: 4212 4274 5004 1090
Size: 62 730 2316
Fragments arranged by size:
2316 1486 730 611 398 190 142 128
93 74 62
BspEI T'CCGG_A
Cuts at: 5450 5450
Size: 6230
BspHI T'CATG_A
Cuts at: 2801 5406 2801
Size: 2605 3625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3280 3494 3796 4177 3280
Size: 214 302 381 5333
Fragments arranged by size:
5333 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4522 4576 6060 6225 50
Size: 4472 54 1484 165 55
Fragments arranged by size:
4472 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 509 4142 509
Size: 3633 2597
BsrFI r'CCGG_y
Cuts at: 4228 4409 4618 4228
Size: 181 209 5840
Fragments arranged by size:
5840 209 181
BsrGI T'GTAC_A
Cuts at: 723 723
Size: 6230
BssSI C'ACGA_G
Cuts at: 1796 4501 4859 1796
Size: 2705 358 3167
Fragments arranged by size:
3167 2705 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1634 3607 1634
Size: 1973 4257
BstBI TT'CG_AA
Cuts at: 110 4591 110
Size: 4481 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 772 772
Size: 6230
BstYI r'GATC_y
Cuts at: 1 522 2571 3026 3255 3722 3745 4080 4326
Size: 521 2049 455 229 467 23 335 246
Cuts at: 4326 5338 5424 5436 5782 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2049 1012 521 467 455 449 346 335
246 229 86 23 12
BstZ17I GTA'TAC
Cuts at: 957 957
Size: 6230
Bsu36I CC'TnA_GG
Cuts at: 3031 3031
Size: 6230
BtgI C'CryG_G
Cuts at: 125 125
Size: 6230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4180 4361 4524 4180
Size: 181 163 5886
Fragments arranged by size:
5886 181 163
BtsI GCAGTG_nn'
Cuts at: 3473 3473
Size: 6230
DraI TTT'AAA
Cuts at: 1727 2487 3141 3229 1727
Size: 760 654 88 4728
Fragments arranged by size:
4728 760 654 88
DrdI GACnn_nn'nnGTC
Cuts at: 1912 3936 4794 1912
Size: 2024 858 3348
Fragments arranged by size:
3348 2024 858
EaeI y'GGCC_r
Cuts at: 128 3815 3989 4380 4407 5752 128
Size: 3687 174 391 27 1345 606
Fragments arranged by size:
3687 1345 606 391 174 27
EagI C'GGCC_G
Cuts at: 3815 3815
Size: 6230
EarI CTCTTCn'nnn_
Cuts at: 299 629 1265 1286 1409 2652 3049 3099 3153
Size: 330 636 21 123 1243 397 50 54
Cuts at: 3153 3193 4253 4463 299
Size: 40 1060 210 2066
Fragments arranged by size:
2066 1243 1060 636 397 330 210 123
54 50 40 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2136 4438 4748 4894 6040 2136
Size: 2302 310 146 1146 2326
Fragments arranged by size:
2326 2302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1486 3242 1486
Size: 1756 4474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 359 1433 1691 2260 2762 3134 4055 4487
Size: 232 1074 258 569 502 372 921 432
Cuts at: 4487 5234 5502 127
Size: 747 268 855
Fragments arranged by size:
1074 921 855 747 569 502 432 372
268 258 232
EcoO109I rG'GnC_Cy
Cuts at: 769 3203 3369 769
Size: 2434 166 3630
Fragments arranged by size:
3630 2434 166
EcoRI G'AATT_C
Cuts at: 1579 3234 1579
Size: 1655 4575
EcoRV GAT'ATC
Cuts at: 2091 2091
Size: 6230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 178 4249 178
Size: 4071 2159
FspI TGC'GCA
Cuts at: 4011 4011
Size: 6230
HaeII r_GCGC'y
Cuts at: 3664 3912 4934 5913 3664
Size: 248 1022 979 3981
Fragments arranged by size:
3981 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 514 546 913 945 1014 1046 1393 1425 2061
Size: 32 367 32 69 32 347 32 636
Cuts at: 2061 2093 2502 2534 2686 2718 4072 4102 4104
Size: 32 409 32 152 32 1354 30 2
Cuts at: 4104 4134 514
Size: 30 2610
Fragments arranged by size:
2610 1354 636 409 367 347 152 69
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 568 927 1022 2738 2945 3269 568
Size: 359 95 1716 207 324 3529
Fragments arranged by size:
3529 1716 359 324 207 95
HpaI GTT'AAC
Cuts at: 927 2738 927
Size: 1811 4419
KasI G'GCGC_C
Cuts at: 3660 3908 3660
Size: 248 5982
KpnI G_GTAC'C
Cuts at: 3250 3250
Size: 6230
MfeI C'AATT_G
Cuts at: 2048 2048
Size: 6230
MluI A'CGCG_T
Cuts at: 5663 5663
Size: 6230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 978 1798 4901 5085 978
Size: 820 3103 184 2123
Fragments arranged by size:
3103 2123 820 184
MscI TGG'CCA
Cuts at: 130 3991 130
Size: 3861 2369
MslI CAynn'nnrTG
Cuts at: 719 884 4219 4346 719
Size: 165 3335 127 2603
Fragments arranged by size:
3335 2603 165 127
MspA1I CmG'CkG
Cuts at: 790 3004 3341 3655 4015 5028 5273 790
Size: 2214 337 314 360 1013 245 1747
Fragments arranged by size:
2214 1747 1013 360 337 314 245
NaeI GCC'GGC
Cuts at: 4411 4411
Size: 6230
NarI GG'CG_CC
Cuts at: 3661 3909 3661
Size: 248 5982
NcoI C'CATG_G
Cuts at: 125 125
Size: 6230
NgoMIV G'CCGG_C
Cuts at: 4409 4409
Size: 6230
NsiI A_TGCA'T
Cuts at: 2223 2250 2223
Size: 27 6203
NspI r_CATG'y
Cuts at: 680 1051 1477 2497 3283 3783 4690 680
Size: 371 426 1020 786 500 907 2220
Fragments arranged by size:
2220 1020 907 786 500 426 371
PciI A'CATG_T
Cuts at: 676 1473 2493 4686 676
Size: 797 1020 2193 2220
Fragments arranged by size:
2220 2193 1020 797
PfoI T'CCnGG_A
Cuts at: 1845 1845
Size: 6230
PmeI GTTT'AAAC
Cuts at: 3229 3229
Size: 6230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1301 1333 5369 5401 1301
Size: 32 4036 32 2130
Fragments arranged by size:
4036 2130 32 32
PpuMI rG'GwC_Cy
Cuts at: 769 3203 769
Size: 2434 3796
PshAI GACnn'nnGTC
Cuts at: 342 342
Size: 6230
PsiI TTA'TAA
Cuts at: 1760 2632 1760
Size: 872 5358
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2854 2886 2854
Size: 32 6198
PstI C_TGCA'G
Cuts at: 282 3277 3962 282
Size: 2995 685 2550
Fragments arranged by size:
2995 2550 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6230
PvuII CAG'CTG
Cuts at: 790 3004 3655 4015 790
Size: 2214 651 360 3005
Fragments arranged by size:
3005 2214 651 360
RsrII CG'GwC_CG
Cuts at: 4425 4425
Size: 6230
SacI G_AGCT'C
Cuts at: 1488 3244 1488
Size: 1756 4474
SalI G'TCGA_C
Cuts at: 3267 3267
Size: 6230
SapI GCTCTTCn'nnn_
Cuts at: 629 3153 4253 4463 629
Size: 2524 1100 210 2396
Fragments arranged by size:
2524 2396 1100 210
SbfI CC_TGCA'GG
Cuts at: 3277 3277
Size: 6230
ScaI AGT'ACT
Cuts at: 5747 5747
Size: 6230
SfcI C'TryA_G
Cuts at: 33 278 3273 3958 4951 5142 33
Size: 245 2995 685 993 191 1121
Fragments arranged by size:
2995 1121 993 685 245 191
SfoI GGC'GCC
Cuts at: 3662 3910 3662
Size: 248 5982
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6230
SmaI CCC'GGG
Cuts at: 3252 3252
Size: 6230
SmlI C'TyrA_G
Cuts at: 1516 3075 3385 4300 4792 5054 1516
Size: 1559 310 915 492 262 2692
Fragments arranged by size:
2692 1559 915 492 310 262
SnaBI TAC'GTA
Cuts at: 3222 3222
Size: 6230
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6230
SphI G_CATG'C
Cuts at: 3283 3783 3283
Size: 500 5730
SspI AAT'ATT
Cuts at: 353 2345 2364 353
Size: 1992 19 4219
Fragments arranged by size:
4219 1992 19
StyI C'CwwG_G
Cuts at: 125 765 1312 3364 3718 125
Size: 640 547 2052 354 2637
Fragments arranged by size:
2637 2052 640 547 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 387 1731 3813 3946 4405 4614 387
Size: 1344 2082 133 459 209 2003
Fragments arranged by size:
2082 2003 1344 459 209 133
TatI w'GTAC_w
Cuts at: 723 1180 1233 2924 5745 723
Size: 457 53 1691 2821 1208
Fragments arranged by size:
2821 1691 1208 457 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5747 5779 5747
Size: 32 6198
Tth111I GACn'n_nGTC
Cuts at: 1960 4027 1960
Size: 2067 4163
XbaI T'CTAG_A
Cuts at: 2224 3261 2224
Size: 1037 5193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 3047 3725 2071
Size: 976 678 4576
Fragments arranged by size:
4576 976 678
XmaI C'CCGG_G
Cuts at: 3250 3250
Size: 6230
XmnI GAAnn'nnTTC
Cuts at: 1256 1805 2001 2059 1256
Size: 549 196 58 5427
Fragments arranged by size:
5427 549 196 58
ZraI GAC'GTC
Cuts at: 1914 1914
Size: 6230
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AlfI AloI ApaI AscI BaeI
BcgI BmtI BsiWI BsmBI BssHII BstEII ClaI CspCI
DraIII EcoNI FseI FspAI HindIII NdeI NheI NotI
NruI PacI PasI PflMI PmlI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI