Restriction map for
pF1KSDA1696
(Circular) (Six-base) MAPSORT of: pF1KSDA1696.seq Check: 9573 from: 1 to: 5039
pF1KSDA1696 5039 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1191 1191
Size: 5039
AatII G_ACGT'C
Cuts at: 673 673
Size: 5039
AccI GT'mk_AC
Cuts at: 1325 1889 2077 1325
Size: 564 188 4287
Fragments arranged by size:
4287 564 188
Acc65I G'GTAC_C
Cuts at: 2055 2055
Size: 5039
AclI AA'CG_TT
Cuts at: 1018 4879 1018
Size: 3861 1178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 2864 3296 4043 4311 1618
Size: 1246 432 747 268 2346
Fragments arranged by size:
2346 1246 747 432 268
AflIII A'CryG_T
Cuts at: 1501 2391 3495 4472 1501
Size: 890 1104 977 2068
Fragments arranged by size:
2068 1104 977 890
AgeI A'CCGG_T
Cuts at: 3427 3427
Size: 5039
AleI CACnn'nnGTG
Cuts at: 640 640
Size: 5039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 470 658 470
Size: 188 4851
AlwNI CAG_nnn'CTG
Cuts at: 183 550 703 1488 1596 2344 3911 183
Size: 367 153 785 108 748 1567 1311
Fragments arranged by size:
1567 1311 785 748 367 153 108
ApaLI G'TGCA_C
Cuts at: 3809 3809
Size: 5039
ApoI r'AATT_y
Cuts at: 164 963 2043 4392 4648 164
Size: 799 1080 2349 256 555
Fragments arranged by size:
2349 1080 799 555 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
AvaI C'yCGr_G
Cuts at: 2059 2059
Size: 5039
BamHI G'GATC_C
Cuts at: 204 2064 204
Size: 1860 3179
BanI G'GyrC_C
Cuts at: 2055 2469 2717 2752 2055
Size: 414 248 35 4342
Fragments arranged by size:
4342 414 248 35
BanII G_rGCy'C
Cuts at: 2053 3083 2053
Size: 1030 4009
BbeI G_GCGC'C
Cuts at: 2473 2721 2473
Size: 248 4791
BbsI GAAGACnn'nnnn_
Cuts at: 493 493
Size: 5039
BbvCI CC'TCA_GC
Cuts at: 758 899 758
Size: 141 4898
BciVI GTATCCnnnnn_n'
Cuts at: 2937 3698 5039 2937
Size: 761 1341 2937
Fragments arranged by size:
2937 1341 761
BclI T'GATC_A
Cuts at: 2559 2559
Size: 5039
BfrBI ATG'CAT
Cuts at: 1778 1778
Size: 5039
BglII A'GATC_T
Cuts at: 2554 4591 2554
Size: 2037 3002
BlpI GC'TnA_GC
Cuts at: 631 1096 1756 2151 631
Size: 465 660 395 3519
Fragments arranged by size:
3519 660 465 395
Bme1580I G_kGCm'C
Cuts at: 2664 2757 3813 2664
Size: 93 1056 3890
Fragments arranged by size:
3890 1056 93
BmgBI CAC'GTC
Cuts at: 808 808
Size: 5039
BmrI ACTGGGnnnn_n'
Cuts at: 695 2251 2332 2667 4811 695
Size: 1556 81 335 2144 923
Fragments arranged by size:
2144 1556 923 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3406 3406
Size: 5039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1043 1852 649
Size: 394 809 3836
Fragments arranged by size:
3836 809 394
Bpu10I CC'TnA_GC
Cuts at: 758 899 758
Size: 141 4898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2215 3094 3586 3884 2215
Size: 879 492 298 3370
Fragments arranged by size:
3370 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 576 4743 576
Size: 4167 872
BsaAI yAC'GTr
Cuts at: 2031 2392 2031
Size: 361 4678
BsaBI GATnn'nnATC
Cuts at: 6 2553 4590 6
Size: 2547 2037 455
Fragments arranged by size:
2547 2037 455
BsaHI Gr'CG_yC
Cuts at: 670 2470 2718 3420 4916 670
Size: 1800 248 702 1496 793
Fragments arranged by size:
1800 1496 793 702 248
BsaWI w'CCGG_w
Cuts at: 330 1320 2228 2749 3427 3701 3848 4259 330
Size: 990 908 521 678 274 147 411 1110
Fragments arranged by size:
1110 990 908 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 746 887 1222 1971 746
Size: 141 335 749 3814
Fragments arranged by size:
3814 749 335 141
BseYI C'CCAG_C
Cuts at: 443 870 1031 2464 3222 3799 4295 443
Size: 427 161 1433 758 577 496 1187
Fragments arranged by size:
1433 1187 758 577 496 427 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1172 1385 1917 1977 664
Size: 508 213 532 60 3726
Fragments arranged by size:
3726 532 508 213 60
BsiEI CG_ry'CG
Cuts at: 104 2581 2627 3835 104
Size: 2477 46 1208 1308
Fragments arranged by size:
2477 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 632 2053 2831 3021 3813 632
Size: 1421 778 190 792 1858
Fragments arranged by size:
1858 1421 792 778 190
BsmI GAATG_Cn'
Cuts at: 219 219
Size: 5039
Bsp1286I G_dGCh'C
Cuts at: 632 2053 2664 2757 2831 3021 3083 3813 632
Size: 1421 611 93 74 190 62 730 1858
Fragments arranged by size:
1858 1421 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4259 4259
Size: 5039
BspHI T'CATG_A
Cuts at: 237 1446 4215 237
Size: 1209 2769 1061
Fragments arranged by size:
2769 1209 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 729 1191 2089 2303 2605 2986 729
Size: 462 898 214 302 381 2782
Fragments arranged by size:
2782 898 462 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3331 3385 4869 5034 50
Size: 3281 54 1484 165 55
Fragments arranged by size:
3281 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 352 590 2951 352
Size: 238 2361 2440
Fragments arranged by size:
2440 2361 238
BsrFI r'CCGG_y
Cuts at: 1965 3037 3218 3427 1965
Size: 1072 181 209 3577
Fragments arranged by size:
3577 1072 209 181
BsrGI T'GTAC_A
Cuts at: 1089 1089
Size: 5039
BssSI C'ACGA_G
Cuts at: 3310 3668 3310
Size: 358 4681
BstAPI GCAn_nnn'nTGC
Cuts at: 550 1466 2416 550
Size: 916 950 3173
Fragments arranged by size:
3173 950 916
BstBI TT'CG_AA
Cuts at: 110 3400 110
Size: 3290 1749
BstEII G'GTnAC_C
Cuts at: 600 600
Size: 5039
BstXI CCAn_nnnn'nTGG
Cuts at: 597 843 597
Size: 246 4793
BstYI r'GATC_y
Cuts at: 1 204 1568 2064 2531 2554 2889 3135 4147
Size: 203 1364 496 467 23 335 246 1012
Cuts at: 4147 4233 4245 4591 1
Size: 86 12 346 449
Fragments arranged by size:
1364 1012 496 467 449 346 335 246
203 86 23 12
BtgI C'CryG_G
Cuts at: 125 1025 1618 1863 125
Size: 900 593 245 3301
Fragments arranged by size:
3301 900 593 245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2989 3170 3333 2989
Size: 181 163 4695
Fragments arranged by size:
4695 181 163
BtsI GCAGTG_nn'
Cuts at: 1373 2282 1373
Size: 909 4130
DraI TTT'AAA
Cuts at: 1715 2038 1715
Size: 323 4716
DrdI GACnn_nn'nnGTC
Cuts at: 2745 3603 2745
Size: 858 4181
EaeI y'GGCC_r
Cuts at: 1429 1479 2624 2798 3189 3216 4561 1429
Size: 50 1145 174 391 27 1345 1907
Fragments arranged by size:
1907 1345 1145 391 174 50 27
EagI C'GGCC_G
Cuts at: 2624 2624
Size: 5039
EarI CTCTTCn'nnn_
Cuts at: 1354 1402 1673 2012 3062 3272 1354
Size: 48 271 339 1050 210 3121
Fragments arranged by size:
3121 1050 339 271 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3247 3557 3703 4849 3247
Size: 310 146 1146 3437
Fragments arranged by size:
3437 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2051 2051
Size: 5039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1043 1618 1852 2864 3296 4043 4311 649
Size: 394 575 234 1012 432 747 268 1377
Fragments arranged by size:
1377 1012 747 575 432 394 268 234
EcoNI CCTnn'n_nnAGG
Cuts at: 1834 1834
Size: 5039
EcoO109I rG'GnC_Cy
Cuts at: 1400 2178 1400
Size: 778 4261
EcoRI G'AATT_C
Cuts at: 963 2043 963
Size: 1080 3959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 5039
FspI TGC'GCA
Cuts at: 2820 2820
Size: 5039
HaeII r_GCGC'y
Cuts at: 2473 2721 3743 4722 2473
Size: 248 1022 979 2790
Fragments arranged by size:
2790 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 513 545 1041 1073 2881 2911 2913 2943 513
Size: 32 496 32 1808 30 2 30 2609
Fragments arranged by size:
2609 1808 496 32 32 30 30 2
HincII GTy'rAC
Cuts at: 660 2078 660
Size: 1418 3621
KasI G'GCGC_C
Cuts at: 2469 2717 2469
Size: 248 4791
KpnI G_GTAC'C
Cuts at: 2059 2059
Size: 5039
MfeI C'AATT_G
Cuts at: 1637 1637
Size: 5039
MluI A'CGCG_T
Cuts at: 4472 4472
Size: 5039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3710 3894 3710
Size: 184 4855
MscI TGG'CCA
Cuts at: 1431 1481 2800 1431
Size: 50 1319 3670
Fragments arranged by size:
3670 1319 50
MslI CAynn'nnrTG
Cuts at: 595 640 1783 3028 3155 595
Size: 45 1143 1245 127 2479
Fragments arranged by size:
2479 1245 1143 127 45
MspA1I CmG'CkG
Cuts at: 454 1848 2150 2464 2824 3837 4082 454
Size: 1394 302 314 360 1013 245 1411
Fragments arranged by size:
1411 1394 1013 360 314 302 245
NaeI GCC'GGC
Cuts at: 1967 3220 1967
Size: 1253 3786
NarI GG'CG_CC
Cuts at: 2470 2718 2470
Size: 248 4791
NcoI C'CATG_G
Cuts at: 125 1025 1618 125
Size: 900 593 3546
Fragments arranged by size:
3546 900 593
NgoMIV G'CCGG_C
Cuts at: 1965 3218 1965
Size: 1253 3786
NsiI A_TGCA'T
Cuts at: 1780 1780
Size: 5039
NspI r_CATG'y
Cuts at: 1505 1566 2092 2592 3499 1505
Size: 61 526 500 907 3045
Fragments arranged by size:
3045 907 526 500 61
PasI CC'CwG_GG
Cuts at: 1396 1396
Size: 5039
PciI A'CATG_T
Cuts at: 1501 3495 1501
Size: 1994 3045
PflMI CCAn_nnn'nTGG
Cuts at: 183 714 1624 183
Size: 531 910 3598
Fragments arranged by size:
3598 910 531
PmeI GTTT'AAAC
Cuts at: 2038 2038
Size: 5039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 4178
Size: 32 5007
PpuMI rG'GwC_Cy
Cuts at: 1400 1400
Size: 5039
PstI C_TGCA'G
Cuts at: 459 740 2086 2771 459
Size: 281 1346 685 2727
Fragments arranged by size:
2727 1346 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5039
PvuII CAG'CTG
Cuts at: 454 1848 2464 2824 454
Size: 1394 616 360 2669
Fragments arranged by size:
2669 1394 616 360
RsrII CG'GwC_CG
Cuts at: 3234 3234
Size: 5039
SacI G_AGCT'C
Cuts at: 2053 2053
Size: 5039
SalI G'TCGA_C
Cuts at: 2076 2076
Size: 5039
SanDI GG'GwC_CC
Cuts at: 1400 1400
Size: 5039
SapI GCTCTTCn'nnn_
Cuts at: 3062 3272 3062
Size: 210 4829
SbfI CC_TGCA'GG
Cuts at: 2086 2086
Size: 5039
ScaI AGT'ACT
Cuts at: 4556 4556
Size: 5039
SfcI C'TryA_G
Cuts at: 33 142 400 455 497 736 1326 1886 2082
Size: 109 258 55 42 239 590 560 196
Cuts at: 2082 2767 3760 3951 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 590 560 258 239 196
191 109 55 42
SfoI GGC'GCC
Cuts at: 2471 2719 2471
Size: 248 4791
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
SmaI CCC'GGG
Cuts at: 2061 2061
Size: 5039
SmlI C'TyrA_G
Cuts at: 2194 3109 3601 3863 2194
Size: 915 492 262 3370
Fragments arranged by size:
3370 915 492 262
SnaBI TAC'GTA
Cuts at: 2031 2031
Size: 5039
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5039
SphI G_CATG'C
Cuts at: 2092 2592 2092
Size: 500 4539
StyI C'CwwG_G
Cuts at: 125 1025 1556 1618 2173 2527 125
Size: 900 531 62 555 354 2637
Fragments arranged by size:
2637 900 555 531 354 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1206 2622 2755 3214 3423 1206
Size: 1416 133 459 209 2822
Fragments arranged by size:
2822 1416 459 209 133
TatI w'GTAC_w
Cuts at: 1089 4554 1089
Size: 3465 1574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 767 792 799 824 933 965 4556 4588 767
Size: 25 7 25 109 32 3591 32 1218
Fragments arranged by size:
3591 1218 109 32 32 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 2836 2836
Size: 5039
XbaI T'CTAG_A
Cuts at: 1266 2070 1266
Size: 804 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2534 2534
Size: 5039
XmaI C'CCGG_G
Cuts at: 2059 2059
Size: 5039
XmnI GAAnn'nnTTC
Cuts at: 1613 1613
Size: 5039
ZraI GAC'GTC
Cuts at: 671 671
Size: 5039
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BcgI BglI BmtI BsaXI BsiWI BsmBI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI