Restriction map for
pF1KSDA1691
(Circular) (Six-base) MAPSORT of: pF1KSDA1691.seq Check: 8928 from: 1 to: 4706
pF1KSDA1691 4706 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 717 717
Size: 4706
AatII G_ACGT'C
Cuts at: 788 788
Size: 4706
AccI GT'mk_AC
Cuts at: 1744 1744
Size: 4706
Acc65I G'GTAC_C
Cuts at: 1722 1722
Size: 4706
AclI AA'CG_TT
Cuts at: 4546 4546
Size: 4706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2963 3710 3978 2531
Size: 432 747 268 3259
Fragments arranged by size:
3259 747 432 268
AfeI AGC'GCT
Cuts at: 1253 1253
Size: 4706
AflIII A'CryG_T
Cuts at: 2058 3162 4139 2058
Size: 1104 977 2625
Fragments arranged by size:
2625 1104 977
AgeI A'CCGG_T
Cuts at: 3094 3094
Size: 4706
AleI CACnn'nnGTG
Cuts at: 409 544 700 1186 409
Size: 135 156 486 3929
Fragments arranged by size:
3929 486 156 135
AlwNI CAG_nnn'CTG
Cuts at: 252 585 636 645 1056 2011 3578 252
Size: 333 51 9 411 955 1567 1380
Fragments arranged by size:
1567 1380 955 411 333 51 9
ApaLI G'TGCA_C
Cuts at: 3476 3476
Size: 4706
ApoI r'AATT_y
Cuts at: 1710 4059 4315 1710
Size: 2349 256 2101
Fragments arranged by size:
2349 2101 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4706
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
AvaI C'yCGr_G
Cuts at: 226 610 1726 226
Size: 384 1116 3206
Fragments arranged by size:
3206 1116 384
BamHI G'GATC_C
Cuts at: 1731 1731
Size: 4706
BanI G'GyrC_C
Cuts at: 147 1722 2136 2384 2419 147
Size: 1575 414 248 35 2434
Fragments arranged by size:
2434 1575 414 248 35
BanII G_rGCy'C
Cuts at: 572 617 925 1720 2750 572
Size: 45 308 795 1030 2528
Fragments arranged by size:
2528 1030 795 308 45
BbeI G_GCGC'C
Cuts at: 151 2140 2388 151
Size: 1989 248 2469
Fragments arranged by size:
2469 1989 248
BciVI GTATCCnnnnn_n'
Cuts at: 2604 3365 4706 2604
Size: 761 1341 2604
Fragments arranged by size:
2604 1341 761
BclI T'GATC_A
Cuts at: 2226 2226
Size: 4706
BglI GCCn_nnn'nGGC
Cuts at: 277 277
Size: 4706
BglII A'GATC_T
Cuts at: 2221 4258 2221
Size: 2037 2669
BlpI GC'TnA_GC
Cuts at: 1103 1818 1103
Size: 715 3991
Bme1580I G_kGCm'C
Cuts at: 2331 2424 3480 2331
Size: 93 1056 3557
Fragments arranged by size:
3557 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1918 1999 2334 4478 1918
Size: 81 335 2144 2146
Fragments arranged by size:
2146 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 996 1685 3073 996
Size: 689 1388 2629
Fragments arranged by size:
2629 1388 689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 670 730 909 1394 1617 1665 607
Size: 63 60 179 485 223 48 3648
Fragments arranged by size:
3648 485 223 179 63 60 48
Bpu10I CC'TnA_GC
Cuts at: 193 193
Size: 4706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 2761 3253 3551 1882
Size: 879 492 298 3037
Fragments arranged by size:
3037 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 448 1486 4410 448
Size: 1038 2924 744
Fragments arranged by size:
2924 1038 744
BsaAI yAC'GTr
Cuts at: 957 1245 1698 2059 957
Size: 288 453 361 3604
Fragments arranged by size:
3604 453 361 288
BsaBI GATnn'nnATC
Cuts at: 6 2220 4257 6
Size: 2214 2037 455
Fragments arranged by size:
2214 2037 455
BsaHI Gr'CG_yC
Cuts at: 148 365 785 950 2137 2385 3087 4583 148
Size: 217 420 165 1187 248 702 1496 271
Fragments arranged by size:
1496 1187 702 420 271 248 217 165
BsaWI w'CCGG_w
Cuts at: 1895 2416 3094 3368 3515 3926 1895
Size: 521 678 274 147 411 2675
Fragments arranged by size:
2675 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 367 988 1144 1411 1414 289
Size: 78 621 156 267 3 3581
Fragments arranged by size:
3581 621 267 156 78 3
BseYI C'CCAG_C
Cuts at: 197 571 660 756 858 884 894 1456 2131
Size: 374 89 96 102 26 10 562 675
Cuts at: 2131 2889 3466 3962 197
Size: 758 577 496 941
Fragments arranged by size:
941 758 675 577 562 496 374 102
96 89 26 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 363 435 154
Size: 209 72 4425
Fragments arranged by size:
4425 209 72
BsiEI CG_ry'CG
Cuts at: 104 674 1508 2248 2294 3502 104
Size: 570 834 740 46 1208 1308
Fragments arranged by size:
1308 1208 834 740 570 46
BsiHKAI G_wGCw'C
Cuts at: 359 1720 2498 2688 3480 359
Size: 1361 778 190 792 1585
Fragments arranged by size:
1585 1361 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 646 646
Size: 4706
Bsp1286I G_dGCh'C
Cuts at: 359 572 617 925 1720 2331 2424 2498 2688
Size: 213 45 308 795 611 93 74 190
Cuts at: 2688 2750 3480 359
Size: 62 730 1585
Fragments arranged by size:
1585 795 730 611 308 213 190 93
74 62 45
BspEI T'CCGG_A
Cuts at: 3926 3926
Size: 4706
BspHI T'CATG_A
Cuts at: 3882 3882
Size: 4706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 717 1202 1756 1970 2272 2653 717
Size: 485 554 214 302 381 2770
Fragments arranged by size:
2770 554 485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 591 1408 2998 3052 4536 4701 50
Size: 541 817 1590 54 1484 165 55
Fragments arranged by size:
1590 1484 817 541 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2618 2618
Size: 4706
BsrFI r'CCGG_y
Cuts at: 173 335 421 1134 1258 2704 2885 3094 173
Size: 162 86 713 124 1446 181 209 1785
Fragments arranged by size:
1785 1446 713 209 181 162 124 86
BsrGI T'GTAC_A
Cuts at: 1463 1463
Size: 4706
BssHII G'CGCG_C
Cuts at: 890 890
Size: 4706
BssSI C'ACGA_G
Cuts at: 1659 2977 3335 1659
Size: 1318 358 3030
Fragments arranged by size:
3030 1318 358
BstAPI GCAn_nnn'nTGC
Cuts at: 177 252 645 2083 177
Size: 75 393 1438 2800
Fragments arranged by size:
2800 1438 393 75
BstBI TT'CG_AA
Cuts at: 110 3067 110
Size: 2957 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 969 969
Size: 4706
BstYI r'GATC_y
Cuts at: 1 732 1731 2198 2221 2556 2802 3814 3900
Size: 731 999 467 23 335 246 1012 86
Cuts at: 3900 3912 4258 1
Size: 12 346 449
Fragments arranged by size:
1012 999 731 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 917 1417 1508 125
Size: 792 500 91 3323
Fragments arranged by size:
3323 792 500 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 384 411 2656 2837 3000 384
Size: 27 2245 181 163 2090
Fragments arranged by size:
2245 2090 181 163 27
BtsI GCAGTG_nn'
Cuts at: 437 1494 1949 437
Size: 1057 455 3194
Fragments arranged by size:
3194 1057 455
DraI TTT'AAA
Cuts at: 1705 1705
Size: 4706
DrdI GACnn_nn'nnGTC
Cuts at: 2412 3270 2412
Size: 858 3848
EaeI y'GGCC_r
Cuts at: 128 512 599 671 1136 1505 2291 2465 2856
Size: 384 87 72 465 369 786 174 391
Cuts at: 2856 2883 4228 128
Size: 27 1345 606
Fragments arranged by size:
1345 786 606 465 391 384 369 174
87 72 27
EagI C'GGCC_G
Cuts at: 671 1505 2291 671
Size: 834 786 3086
Fragments arranged by size:
3086 834 786
EarI CTCTTCn'nnn_
Cuts at: 307 316 1309 2729 2939 307
Size: 9 993 1420 210 2074
Fragments arranged by size:
2074 1420 993 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 481 580 736 2914 3224 3370 4516 481
Size: 99 156 2178 310 146 1146 671
Fragments arranged by size:
2178 1146 671 310 156 146 99
EcoICRI GAG'CTC
Cuts at: 1718 1718
Size: 4706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 670 730 909 1394 1617 1665 2531 2963
Size: 63 60 179 485 223 48 866 432
Cuts at: 2963 3710 3978 607
Size: 747 268 1335
Fragments arranged by size:
1335 866 747 485 432 268 223 179
63 60 48
EcoNI CCTnn'n_nnAGG
Cuts at: 687 687
Size: 4706
EcoO109I rG'GnC_Cy
Cuts at: 249 642 1146 1284 1378 1845 249
Size: 393 504 138 94 467 3110
Fragments arranged by size:
3110 504 467 393 138 94
EcoRI G'AATT_C
Cuts at: 1710 1710
Size: 4706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4706
FspI TGC'GCA
Cuts at: 2487 2487
Size: 4706
HaeII r_GCGC'y
Cuts at: 151 422 1255 2140 2388 3410 4389 151
Size: 271 833 885 248 1022 979 468
Fragments arranged by size:
1022 979 885 833 468 271 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2548 2578 2580 2610 2548
Size: 30 2 30 4644
Fragments arranged by size:
4644 30 30 2
HincII GTy'rAC
Cuts at: 1745 1745
Size: 4706
KasI G'GCGC_C
Cuts at: 147 2136 2384 147
Size: 1989 248 2469
Fragments arranged by size:
2469 1989 248
KpnI G_GTAC'C
Cuts at: 1726 1726
Size: 4706
MluI A'CGCG_T
Cuts at: 4139 4139
Size: 4706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3377 3561 3377
Size: 184 4522
MscI TGG'CCA
Cuts at: 2467 2467
Size: 4706
MslI CAynn'nnrTG
Cuts at: 409 430 544 700 841 1186 2695 2822 409
Size: 21 114 156 141 345 1509 127 2293
Fragments arranged by size:
2293 1509 345 156 141 127 114 21
MspA1I CmG'CkG
Cuts at: 597 884 1419 1469 1510 1580 1685 1817 2131
Size: 287 535 50 41 70 105 132 314
Cuts at: 2131 2491 3504 3749 597
Size: 360 1013 245 1554
Fragments arranged by size:
1554 1013 535 360 314 287 245 132
105 70 50 41
NaeI GCC'GGC
Cuts at: 337 423 1136 2887 337
Size: 86 713 1751 2156
Fragments arranged by size:
2156 1751 713 86
NarI GG'CG_CC
Cuts at: 148 2137 2385 148
Size: 1989 248 2469
Fragments arranged by size:
2469 1989 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4706
NgoMIV G'CCGG_C
Cuts at: 335 421 1134 2885 335
Size: 86 713 1751 2156
Fragments arranged by size:
2156 1751 713 86
NspI r_CATG'y
Cuts at: 1455 1759 2259 3166 1455
Size: 304 500 907 2995
Fragments arranged by size:
2995 907 500 304
PasI CC'CwG_GG
Cuts at: 1124 1124
Size: 4706
PciI A'CATG_T
Cuts at: 3162 3162
Size: 4706
PflMI CCAn_nnn'nTGG
Cuts at: 1600 1600
Size: 4706
PfoI T'CCnGG_A
Cuts at: 521 1163 521
Size: 642 4064
PmeI GTTT'AAAC
Cuts at: 1705 1705
Size: 4706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 4674
PpuMI rG'GwC_Cy
Cuts at: 1146 1146
Size: 4706
PstI C_TGCA'G
Cuts at: 581 641 1007 1199 1348 1753 2438 581
Size: 60 366 192 149 405 685 2849
Fragments arranged by size:
2849 685 405 366 192 149 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4706
PvuII CAG'CTG
Cuts at: 597 884 1469 2131 2491 597
Size: 287 585 662 360 2812
Fragments arranged by size:
2812 662 585 360 287
RsrII CG'GwC_CG
Cuts at: 2901 2901
Size: 4706
SacI G_AGCT'C
Cuts at: 1720 1720
Size: 4706
SacII CC_GC'GG
Cuts at: 1420 1511 1420
Size: 91 4615
SalI G'TCGA_C
Cuts at: 1743 1743
Size: 4706
SapI GCTCTTCn'nnn_
Cuts at: 316 2729 2939 316
Size: 2413 210 2083
Fragments arranged by size:
2413 2083 210
SbfI CC_TGCA'GG
Cuts at: 1753 1753
Size: 4706
ScaI AGT'ACT
Cuts at: 979 1009 4223 979
Size: 30 3214 1462
Fragments arranged by size:
3214 1462 30
SfcI C'TryA_G
Cuts at: 33 577 637 1003 1195 1344 1749 2434 3427
Size: 544 60 366 192 149 405 685 993
Cuts at: 3427 3618 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 544 405 366 192 191
149 60
SfoI GGC'GCC
Cuts at: 149 2138 2386 149
Size: 1989 248 2469
Fragments arranged by size:
2469 1989 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
SmaI CCC'GGG
Cuts at: 1728 1728
Size: 4706
SmlI C'TyrA_G
Cuts at: 1861 2776 3268 3530 1861
Size: 915 492 262 3037
Fragments arranged by size:
3037 915 492 262
SnaBI TAC'GTA
Cuts at: 1698 1698
Size: 4706
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4706
SphI G_CATG'C
Cuts at: 1759 2259 1759
Size: 500 4206
StyI C'CwwG_G
Cuts at: 125 830 1840 2194 125
Size: 705 1010 354 2637
Fragments arranged by size:
2637 1010 705 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 147 2289 2422 2881 3090 147
Size: 2142 133 459 209 1763
Fragments arranged by size:
2142 1763 459 209 133
TatI w'GTAC_w
Cuts at: 977 1007 1463 1544 4221 977
Size: 30 456 81 2677 1462
Fragments arranged by size:
2677 1462 456 81 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 4223 4255 517
Size: 32 3674 32 968
Fragments arranged by size:
3674 968 32 32
Tth111I GACn'n_nGTC
Cuts at: 2503 2503
Size: 4706
XbaI T'CTAG_A
Cuts at: 1737 1737
Size: 4706
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 507 837 2201 507
Size: 330 1364 3012
Fragments arranged by size:
3012 1364 330
XmaI C'CCGG_G
Cuts at: 1726 1726
Size: 4706
ZraI GAC'GTC
Cuts at: 786 786
Size: 4706
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI