(Circular) (Six-base) MAPSORT of: pF1KSDA1663.seq Check: 6916 from: 1 to: 4169 pF1KSDA1663 4169 bp With 182 enzymes: SgfI * June 20, 2008 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1012 1012 Size: 4169 AccI GT'mk_AC Cuts at: 1207 1207 Size: 4169 Acc65I G'GTAC_C Cuts at: 257 1185 257 Size: 928 3241 AclI AA'CG_TT Cuts at: 4009 4009 Size: 4169 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 829 1994 2426 3173 3441 829 Size: 1165 432 747 268 1557 Fragments arranged by size: 1557 1165 747 432 268 AflIII A'CryG_T Cuts at: 1521 2625 3602 1521 Size: 1104 977 2088 Fragments arranged by size: 2088 1104 977 AgeI A'CCGG_T Cuts at: 2557 2557 Size: 4169 AlwNI CAG_nnn'CTG Cuts at: 1474 3041 1474 Size: 1567 2602 ApaLI G'TGCA_C Cuts at: 2939 2939 Size: 4169 ApoI r'AATT_y Cuts at: 679 1173 3522 3778 679 Size: 494 2349 256 1070 Fragments arranged by size: 2349 1070 494 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4169 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4169 AvaI C'yCGr_G Cuts at: 1189 1189 Size: 4169 BamHI G'GATC_C Cuts at: 1194 1194 Size: 4169 BanI G'GyrC_C Cuts at: 191 257 487 967 1000 1048 1185 1599 1847 Size: 66 230 480 33 48 137 414 248 Cuts at: 1847 1882 191 Size: 35 2478 Fragments arranged by size: 2478 480 414 248 230 137 66 48 35 33 BanII G_rGCy'C Cuts at: 1183 2213 1183 Size: 1030 3139 BbeI G_GCGC'C Cuts at: 195 1603 1851 195 Size: 1408 248 2513 Fragments arranged by size: 2513 1408 248 BbsI GAAGACnn'nnnn_ Cuts at: 1078 1078 Size: 4169 BbvCI CC'TCA_GC Cuts at: 1093 1093 Size: 4169 BciVI GTATCCnnnnn_n' Cuts at: 1130 2067 2828 4169 1130 Size: 937 761 1341 1130 Fragments arranged by size: 1341 1130 937 761 BclI T'GATC_A Cuts at: 1689 1689 Size: 4169 BfrBI ATG'CAT Cuts at: 837 837 Size: 4169 BglI GCCn_nnn'nGGC Cuts at: 865 865 Size: 4169 BglII A'GATC_T Cuts at: 1684 3721 1684 Size: 2037 2132 BlpI GC'TnA_GC Cuts at: 638 1281 638 Size: 643 3526 Bme1580I G_kGCm'C Cuts at: 492 551 1794 1887 2943 492 Size: 59 1243 93 1056 1718 Fragments arranged by size: 1718 1243 1056 93 59 BmrI ACTGGGnnnn_n' Cuts at: 1381 1462 1797 3941 1381 Size: 81 335 2144 1609 Fragments arranged by size: 2144 1609 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 842 2536 842 Size: 1694 2475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1087 1087 Size: 4169 Bpu10I CC'TnA_GC Cuts at: 1093 1093 Size: 4169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1345 2224 2716 3014 1345 Size: 879 492 298 2500 Fragments arranged by size: 2500 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3873 3873 Size: 4169 BsaAI yAC'GTr Cuts at: 1161 1522 1161 Size: 361 3808 BsaBI GATnn'nnATC Cuts at: 6 1683 3720 6 Size: 1677 2037 455 Fragments arranged by size: 2037 1677 455 BsaHI Gr'CG_yC Cuts at: 192 533 1600 1848 2550 4046 192 Size: 341 1067 248 702 1496 315 Fragments arranged by size: 1496 1067 702 341 315 248 BsaWI w'CCGG_w Cuts at: 1358 1879 2557 2831 2978 3389 1358 Size: 521 678 274 147 411 2138 Fragments arranged by size: 2138 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 892 922 892 Size: 30 4139 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 229 403 229 Size: 174 3995 BseYI C'CCAG_C Cuts at: 610 878 928 1123 1594 2352 2929 3425 610 Size: 268 50 195 471 758 577 496 1354 Fragments arranged by size: 1354 758 577 496 471 268 195 50 BsiEI CG_ry'CG Cuts at: 104 950 1711 1757 2965 104 Size: 846 761 46 1208 1308 Fragments arranged by size: 1308 1208 846 761 46 BsiHKAI G_wGCw'C Cuts at: 1183 1961 2151 2943 1183 Size: 778 190 792 2409 Fragments arranged by size: 2409 792 778 190 BsmI GAATG_Cn' Cuts at: 837 837 Size: 4169 Bsp1286I G_dGCh'C Cuts at: 492 551 1183 1794 1887 1961 2151 2213 2943 Size: 59 632 611 93 74 190 62 730 Cuts at: 2943 492 Size: 1718 Fragments arranged by size: 1718 730 632 611 190 93 74 62 59 BspEI T'CCGG_A Cuts at: 3389 3389 Size: 4169 BspHI T'CATG_A Cuts at: 3345 3345 Size: 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1012 1219 1433 1735 2116 1012 Size: 207 214 302 381 3065 Fragments arranged by size: 3065 381 302 214 207 BsrBI CCG'CTC Cuts at: 50 225 632 637 788 2461 2515 3999 4164 Size: 175 407 5 151 1673 54 1484 165 Cuts at: 4164 50 Size: 55 Fragments arranged by size: 1673 1484 407 175 165 151 55 54 5 BsrDI GCAATG_nn' Cuts at: 2081 2081 Size: 4169 BsrFI r'CCGG_y Cuts at: 2167 2348 2557 2167 Size: 181 209 3779 Fragments arranged by size: 3779 209 181 BsrGI T'GTAC_A Cuts at: 173 908 173 Size: 735 3434 BssSI C'ACGA_G Cuts at: 2440 2798 2440 Size: 358 3811 BstAPI GCAn_nnn'nTGC Cuts at: 1546 1546 Size: 4169 BstBI TT'CG_AA Cuts at: 110 2530 110 Size: 2420 1749 BstXI CCAn_nnnn'nTGG Cuts at: 1039 1039 Size: 4169 BstYI r'GATC_y Cuts at: 1 1194 1661 1684 2019 2265 3277 3363 3375 Size: 1193 467 23 335 246 1012 86 12 Cuts at: 3375 3721 1 Size: 346 449 Fragments arranged by size: 1193 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 386 386 Size: 4169 BtgI C'CryG_G Cuts at: 323 1142 323 Size: 819 3350 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 572 2119 2300 2463 572 Size: 1547 181 163 2278 Fragments arranged by size: 2278 1547 181 163 BtsI GCAGTG_nn' Cuts at: 747 996 1412 747 Size: 249 416 3504 Fragments arranged by size: 3504 416 249 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 330 365 663 698 900 935 330 Size: 35 298 35 202 35 3564 Fragments arranged by size: 3564 298 202 35 35 35 DraI TTT'AAA Cuts at: 1168 1168 Size: 4169 DrdI GACnn_nn'nnGTC Cuts at: 1875 2733 1875 Size: 858 3311 EaeI y'GGCC_r Cuts at: 335 600 753 857 947 954 1151 1754 1928 Size: 265 153 104 90 7 197 603 174 Cuts at: 1928 2319 2346 3691 335 Size: 391 27 1345 813 Fragments arranged by size: 1345 813 603 391 265 197 174 153 104 90 27 7 EagI C'GGCC_G Cuts at: 947 1754 947 Size: 807 3362 EarI CTCTTCn'nnn_ Cuts at: 940 1066 2192 2402 940 Size: 126 1126 210 2707 Fragments arranged by size: 2707 1126 210 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2377 2687 2833 3979 2377 Size: 310 146 1146 2567 Fragments arranged by size: 2567 1146 310 146 EcoICRI GAG'CTC Cuts at: 1181 1181 Size: 4169 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 829 1087 1994 2426 3173 3441 829 Size: 258 907 432 747 268 1557 Fragments arranged by size: 1557 907 747 432 268 258 EcoO109I rG'GnC_Cy Cuts at: 1308 1308 Size: 4169 EcoRI G'AATT_C Cuts at: 1173 1173 Size: 4169 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 537 1095 2188 537 Size: 558 1093 2518 Fragments arranged by size: 2518 1093 558 FspI TGC'GCA Cuts at: 1950 1950 Size: 4169 HaeII r_GCGC'y Cuts at: 195 1603 1851 2873 3852 195 Size: 1408 248 1022 979 512 Fragments arranged by size: 1408 1022 979 512 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2011 2041 2043 2073 2011 Size: 30 2 30 4107 Fragments arranged by size: 4107 30 30 2 HincII GTy'rAC Cuts at: 1208 1208 Size: 4169 KasI G'GCGC_C Cuts at: 191 1599 1847 191 Size: 1408 248 2513 Fragments arranged by size: 2513 1408 248 KpnI G_GTAC'C Cuts at: 261 1189 261 Size: 928 3241 MluI A'CGCG_T Cuts at: 3602 3602 Size: 4169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 602 2840 3024 602 Size: 2238 184 1747 Fragments arranged by size: 2238 1747 184 MscI TGG'CCA Cuts at: 602 755 956 1153 1930 602 Size: 153 201 197 777 2841 Fragments arranged by size: 2841 777 201 197 153 MslI CAynn'nnrTG Cuts at: 694 2158 2285 694 Size: 1464 127 2578 Fragments arranged by size: 2578 1464 127 MspA1I CmG'CkG Cuts at: 293 965 1280 1594 1954 2967 3212 293 Size: 672 315 314 360 1013 245 1250 Fragments arranged by size: 1250 1013 672 360 315 314 245 NaeI GCC'GGC Cuts at: 2350 2350 Size: 4169 NarI GG'CG_CC Cuts at: 192 1600 1848 192 Size: 1408 248 2513 Fragments arranged by size: 2513 1408 248 NgoMIV G'CCGG_C Cuts at: 2348 2348 Size: 4169 NsiI A_TGCA'T Cuts at: 839 839 Size: 4169 NspI r_CATG'y Cuts at: 1222 1722 2629 1222 Size: 500 907 2762 Fragments arranged by size: 2762 907 500 PciI A'CATG_T Cuts at: 2625 2625 Size: 4169 PflMI CCAn_nnn'nTGG Cuts at: 598 1143 598 Size: 545 3624 PfoI T'CCnGG_A Cuts at: 1064 1064 Size: 4169 PmeI GTTT'AAAC Cuts at: 1168 1168 Size: 4169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3308 3340 3308 Size: 32 4137 PstI C_TGCA'G Cuts at: 1216 1901 1216 Size: 685 3484 PvuI CG_AT'CG Cuts at: 104 104 Size: 4169 PvuII CAG'CTG Cuts at: 1594 1954 1594 Size: 360 3809 RsrII CG'GwC_CG Cuts at: 2364 2364 Size: 4169 SacI G_AGCT'C Cuts at: 1183 1183 Size: 4169 SalI G'TCGA_C Cuts at: 1206 1206 Size: 4169 SapI GCTCTTCn'nnn_ Cuts at: 2192 2402 2192 Size: 210 3959 SbfI CC_TGCA'GG Cuts at: 1216 1216 Size: 4169 ScaI AGT'ACT Cuts at: 3686 3686 Size: 4169 SfcI C'TryA_G Cuts at: 33 1212 1897 2890 3081 33 Size: 1179 685 993 191 1121 Fragments arranged by size: 1179 1121 993 685 191 SfiI GGCCn_nnn'nGGCC Cuts at: 865 865 Size: 4169 SfoI GGC'GCC Cuts at: 193 1601 1849 193 Size: 1408 248 2513 Fragments arranged by size: 2513 1408 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4169 SmaI CCC'GGG Cuts at: 1191 1191 Size: 4169 SmlI C'TyrA_G Cuts at: 1324 2239 2731 2993 1324 Size: 915 492 262 2500 Fragments arranged by size: 2500 915 492 262 SnaBI TAC'GTA Cuts at: 1161 1161 Size: 4169 SpeI A'CTAG_T Cuts at: 67 67 Size: 4169 SphI G_CATG'C Cuts at: 1222 1722 1222 Size: 500 3669 StuI AGG'CCT Cuts at: 1091 1091 Size: 4169 StyI C'CwwG_G Cuts at: 896 1303 1657 896 Size: 407 354 3408 Fragments arranged by size: 3408 407 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 624 807 903 942 1752 1885 2344 2553 624 Size: 183 96 39 810 133 459 209 2240 Fragments arranged by size: 2240 810 459 209 183 133 96 39 TatI w'GTAC_w Cuts at: 173 908 3684 173 Size: 735 2776 658 Fragments arranged by size: 2776 735 658 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3686 3718 3686 Size: 32 4137 Tth111I GACn'n_nGTC Cuts at: 1966 1966 Size: 4169 XbaI T'CTAG_A Cuts at: 1200 1200 Size: 4169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1664 1664 Size: 4169 XmaI C'CCGG_G Cuts at: 1189 1189 Size: 4169 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BmgBI BmtI BsgI BsiWI BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SrfI SspI SwaI XhoI XmnI ZraI