Restriction map for pF1KSDA1663
 (Circular) (Six-base) MAPSORT of: pF1KSDA1663.seq  Check: 6916  from: 1  to: 4169



pF1KSDA1663  4169 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1012    1012 
   Size:       4169
 
AccI GT'mk_AC

Cuts at:   1207    1207 
   Size:       4169
 
Acc65I G'GTAC_C

Cuts at:    257    1185     257 
   Size:        928    3241
 
AclI AA'CG_TT

Cuts at:   4009    4009 
   Size:       4169
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    829    1994    2426    3173    3441     829 
   Size:       1165     432     747     268    1557

  Fragments arranged by size: 

                1557    1165     747     432     268
 
AflIII A'CryG_T

Cuts at:   1521    2625    3602    1521 
   Size:       1104     977    2088

  Fragments arranged by size: 

                2088    1104     977
 
AgeI A'CCGG_T

Cuts at:   2557    2557 
   Size:       4169
 
AlwNI CAG_nnn'CTG

Cuts at:   1474    3041    1474 
   Size:       1567    2602
 
ApaLI G'TGCA_C

Cuts at:   2939    2939 
   Size:       4169
 
ApoI r'AATT_y

Cuts at:    679    1173    3522    3778     679 
   Size:        494    2349     256    1070

  Fragments arranged by size: 

                2349    1070     494     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4169
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4169
 
AvaI C'yCGr_G

Cuts at:   1189    1189 
   Size:       4169
 
BamHI G'GATC_C

Cuts at:   1194    1194 
   Size:       4169
 
BanI G'GyrC_C

Cuts at:    191     257     487     967    1000    1048    1185    1599    1847 
   Size:         66     230     480      33      48     137     414     248

Cuts at:   1847    1882     191 
   Size:         35    2478

  Fragments arranged by size: 

                2478     480     414     248     230     137      66      48
                  35      33
 
BanII G_rGCy'C

Cuts at:   1183    2213    1183 
   Size:       1030    3139
 
BbeI G_GCGC'C

Cuts at:    195    1603    1851     195 
   Size:       1408     248    2513

  Fragments arranged by size: 

                2513    1408     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1078    1078 
   Size:       4169
 
BbvCI CC'TCA_GC

Cuts at:   1093    1093 
   Size:       4169
 
BciVI GTATCCnnnnn_n'

Cuts at:   1130    2067    2828    4169    1130 
   Size:        937     761    1341    1130

  Fragments arranged by size: 

                1341    1130     937     761
 
BclI T'GATC_A

Cuts at:   1689    1689 
   Size:       4169
 
BfrBI ATG'CAT

Cuts at:    837     837 
   Size:       4169
 
BglI GCCn_nnn'nGGC

Cuts at:    865     865 
   Size:       4169
 
BglII A'GATC_T

Cuts at:   1684    3721    1684 
   Size:       2037    2132
 
BlpI GC'TnA_GC

Cuts at:    638    1281     638 
   Size:        643    3526
 
Bme1580I G_kGCm'C

Cuts at:    492     551    1794    1887    2943     492 
   Size:         59    1243      93    1056    1718

  Fragments arranged by size: 

                1718    1243    1056      93      59
 
BmrI ACTGGGnnnn_n'

Cuts at:   1381    1462    1797    3941    1381 
   Size:         81     335    2144    1609

  Fragments arranged by size: 

                2144    1609     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    842    2536     842 
   Size:       1694    2475
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1087    1087 
   Size:       4169
 
Bpu10I CC'TnA_GC

Cuts at:   1093    1093 
   Size:       4169
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1345    2224    2716    3014    1345 
   Size:        879     492     298    2500

  Fragments arranged by size: 

                2500     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3873    3873 
   Size:       4169
 
BsaAI yAC'GTr

Cuts at:   1161    1522    1161 
   Size:        361    3808
 
BsaBI GATnn'nnATC

Cuts at:      6    1683    3720       6 
   Size:       1677    2037     455

  Fragments arranged by size: 

                2037    1677     455
 
BsaHI Gr'CG_yC

Cuts at:    192     533    1600    1848    2550    4046     192 
   Size:        341    1067     248     702    1496     315

  Fragments arranged by size: 

                1496    1067     702     341     315     248
 
BsaWI w'CCGG_w

Cuts at:   1358    1879    2557    2831    2978    3389    1358 
   Size:        521     678     274     147     411    2138

  Fragments arranged by size: 

                2138     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    892     922     892 
   Size:         30    4139
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    229     403     229 
   Size:        174    3995
 
BseYI C'CCAG_C

Cuts at:    610     878     928    1123    1594    2352    2929    3425     610 
   Size:        268      50     195     471     758     577     496    1354

  Fragments arranged by size: 

                1354     758     577     496     471     268     195      50
            
 
BsiEI CG_ry'CG

Cuts at:    104     950    1711    1757    2965     104 
   Size:        846     761      46    1208    1308

  Fragments arranged by size: 

                1308    1208     846     761      46
 
BsiHKAI G_wGCw'C

Cuts at:   1183    1961    2151    2943    1183 
   Size:        778     190     792    2409

  Fragments arranged by size: 

                2409     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    837     837 
   Size:       4169
 
Bsp1286I G_dGCh'C

Cuts at:    492     551    1183    1794    1887    1961    2151    2213    2943 
   Size:         59     632     611      93      74     190      62     730

Cuts at:   2943     492 
   Size:       1718

  Fragments arranged by size: 

                1718     730     632     611     190      93      74      62
                  59
 
BspEI T'CCGG_A

Cuts at:   3389    3389 
   Size:       4169
 
BspHI T'CATG_A

Cuts at:   3345    3345 
   Size:       4169
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1012    1219    1433    1735    2116    1012 
   Size:        207     214     302     381    3065

  Fragments arranged by size: 

                3065     381     302     214     207
 
BsrBI CCG'CTC

Cuts at:     50     225     632     637     788    2461    2515    3999    4164 
   Size:        175     407       5     151    1673      54    1484     165

Cuts at:   4164      50 
   Size:         55

  Fragments arranged by size: 

                1673    1484     407     175     165     151      55      54
                   5
 
BsrDI GCAATG_nn'

Cuts at:   2081    2081 
   Size:       4169
 
BsrFI r'CCGG_y

Cuts at:   2167    2348    2557    2167 
   Size:        181     209    3779

  Fragments arranged by size: 

                3779     209     181
 
BsrGI T'GTAC_A

Cuts at:    173     908     173 
   Size:        735    3434
 
BssSI C'ACGA_G

Cuts at:   2440    2798    2440 
   Size:        358    3811
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1546    1546 
   Size:       4169
 
BstBI TT'CG_AA

Cuts at:    110    2530     110 
   Size:       2420    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1039    1039 
   Size:       4169
 
BstYI r'GATC_y

Cuts at:      1    1194    1661    1684    2019    2265    3277    3363    3375 
   Size:       1193     467      23     335     246    1012      86      12

Cuts at:   3375    3721       1 
   Size:        346     449

  Fragments arranged by size: 

                1193    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    386     386 
   Size:       4169
 
BtgI C'CryG_G

Cuts at:    323    1142     323 
   Size:        819    3350
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    572    2119    2300    2463     572 
   Size:       1547     181     163    2278

  Fragments arranged by size: 

                2278    1547     181     163
 
BtsI GCAGTG_nn'

Cuts at:    747     996    1412     747 
   Size:        249     416    3504

  Fragments arranged by size: 

                3504     416     249
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    330     365     663     698     900     935     330 
   Size:         35     298      35     202      35    3564

  Fragments arranged by size: 

                3564     298     202      35      35      35
 
DraI TTT'AAA

Cuts at:   1168    1168 
   Size:       4169
 
DrdI GACnn_nn'nnGTC

Cuts at:   1875    2733    1875 
   Size:        858    3311
 
EaeI y'GGCC_r

Cuts at:    335     600     753     857     947     954    1151    1754    1928 
   Size:        265     153     104      90       7     197     603     174

Cuts at:   1928    2319    2346    3691     335 
   Size:        391      27    1345     813

  Fragments arranged by size: 

                1345     813     603     391     265     197     174     153
                 104      90      27       7
 
EagI C'GGCC_G

Cuts at:    947    1754     947 
   Size:        807    3362
 
EarI CTCTTCn'nnn_

Cuts at:    940    1066    2192    2402     940 
   Size:        126    1126     210    2707

  Fragments arranged by size: 

                2707    1126     210     126
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2377    2687    2833    3979    2377 
   Size:        310     146    1146    2567

  Fragments arranged by size: 

                2567    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1181    1181 
   Size:       4169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    829    1087    1994    2426    3173    3441     829 
   Size:        258     907     432     747     268    1557

  Fragments arranged by size: 

                1557     907     747     432     268     258
 
EcoO109I rG'GnC_Cy

Cuts at:   1308    1308 
   Size:       4169
 
EcoRI G'AATT_C

Cuts at:   1173    1173 
   Size:       4169
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    537    1095    2188     537 
   Size:        558    1093    2518

  Fragments arranged by size: 

                2518    1093     558
 
FspI TGC'GCA

Cuts at:   1950    1950 
   Size:       4169
 
HaeII r_GCGC'y

Cuts at:    195    1603    1851    2873    3852     195 
   Size:       1408     248    1022     979     512

  Fragments arranged by size: 

                1408    1022     979     512     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2011    2041    2043    2073    2011 
   Size:         30       2      30    4107

  Fragments arranged by size: 

                4107      30      30       2
 
HincII GTy'rAC

Cuts at:   1208    1208 
   Size:       4169
 
KasI G'GCGC_C

Cuts at:    191    1599    1847     191 
   Size:       1408     248    2513

  Fragments arranged by size: 

                2513    1408     248
 
KpnI G_GTAC'C

Cuts at:    261    1189     261 
   Size:        928    3241
 
MluI A'CGCG_T

Cuts at:   3602    3602 
   Size:       4169
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    602    2840    3024     602 
   Size:       2238     184    1747

  Fragments arranged by size: 

                2238    1747     184
 
MscI TGG'CCA

Cuts at:    602     755     956    1153    1930     602 
   Size:        153     201     197     777    2841

  Fragments arranged by size: 

                2841     777     201     197     153
 
MslI CAynn'nnrTG

Cuts at:    694    2158    2285     694 
   Size:       1464     127    2578

  Fragments arranged by size: 

                2578    1464     127
 
MspA1I CmG'CkG

Cuts at:    293     965    1280    1594    1954    2967    3212     293 
   Size:        672     315     314     360    1013     245    1250

  Fragments arranged by size: 

                1250    1013     672     360     315     314     245
 
NaeI GCC'GGC

Cuts at:   2350    2350 
   Size:       4169
 
NarI GG'CG_CC

Cuts at:    192    1600    1848     192 
   Size:       1408     248    2513

  Fragments arranged by size: 

                2513    1408     248
 
NgoMIV G'CCGG_C

Cuts at:   2348    2348 
   Size:       4169
 
NsiI A_TGCA'T

Cuts at:    839     839 
   Size:       4169
 
NspI r_CATG'y

Cuts at:   1222    1722    2629    1222 
   Size:        500     907    2762

  Fragments arranged by size: 

                2762     907     500
 
PciI A'CATG_T

Cuts at:   2625    2625 
   Size:       4169
 
PflMI CCAn_nnn'nTGG

Cuts at:    598    1143     598 
   Size:        545    3624
 
PfoI T'CCnGG_A

Cuts at:   1064    1064 
   Size:       4169
 
PmeI GTTT'AAAC

Cuts at:   1168    1168 
   Size:       4169
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3308    3340    3308 
   Size:         32    4137
 
PstI C_TGCA'G

Cuts at:   1216    1901    1216 
   Size:        685    3484
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4169
 
PvuII CAG'CTG

Cuts at:   1594    1954    1594 
   Size:        360    3809
 
RsrII CG'GwC_CG

Cuts at:   2364    2364 
   Size:       4169
 
SacI G_AGCT'C

Cuts at:   1183    1183 
   Size:       4169
 
SalI G'TCGA_C

Cuts at:   1206    1206 
   Size:       4169
 
SapI GCTCTTCn'nnn_

Cuts at:   2192    2402    2192 
   Size:        210    3959
 
SbfI CC_TGCA'GG

Cuts at:   1216    1216 
   Size:       4169
 
ScaI AGT'ACT

Cuts at:   3686    3686 
   Size:       4169
 
SfcI C'TryA_G

Cuts at:     33    1212    1897    2890    3081      33 
   Size:       1179     685     993     191    1121

  Fragments arranged by size: 

                1179    1121     993     685     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    865     865 
   Size:       4169
 
SfoI GGC'GCC

Cuts at:    193    1601    1849     193 
   Size:       1408     248    2513

  Fragments arranged by size: 

                2513    1408     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4169
 
SmaI CCC'GGG

Cuts at:   1191    1191 
   Size:       4169
 
SmlI C'TyrA_G

Cuts at:   1324    2239    2731    2993    1324 
   Size:        915     492     262    2500

  Fragments arranged by size: 

                2500     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1161    1161 
   Size:       4169
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4169
 
SphI G_CATG'C

Cuts at:   1222    1722    1222 
   Size:        500    3669
 
StuI AGG'CCT

Cuts at:   1091    1091 
   Size:       4169
 
StyI C'CwwG_G

Cuts at:    896    1303    1657     896 
   Size:        407     354    3408

  Fragments arranged by size: 

                3408     407     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    624     807     903     942    1752    1885    2344    2553     624 
   Size:        183      96      39     810     133     459     209    2240

  Fragments arranged by size: 

                2240     810     459     209     183     133      96      39
            
 
TatI w'GTAC_w

Cuts at:    173     908    3684     173 
   Size:        735    2776     658

  Fragments arranged by size: 

                2776     735     658
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3686    3718    3686 
   Size:         32    4137
 
Tth111I GACn'n_nGTC

Cuts at:   1966    1966 
   Size:       4169
 
XbaI T'CTAG_A

Cuts at:   1200    1200 
   Size:       4169
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1664    1664 
   Size:       4169
 
XmaI C'CCGG_G

Cuts at:   1189    1189 
   Size:       4169
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI     PciI
    PflMI     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BcgI    BmgBI     BmtI     BsgI    BsiWI
    BsmBI   BssHII   BstEII  BstZ17I     ClaI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     PacI     PasI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI    SgrAI     SrfI
     SspI     SwaI     XhoI     XmnI     ZraI
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