Restriction map for
pF1KSDA1627
(Circular) (Six-base) MAPSORT of: pF1KSDA1627.seq Check: 8200 from: 1 to: 4505
pF1KSDA1627 4505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 497 1543 497
Size: 1046 3459
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2762 3509 3777 2330
Size: 432 747 268 3058
Fragments arranged by size:
3058 747 432 268
AflII C'TTAA_G
Cuts at: 445 445
Size: 4505
AflIII A'CryG_T
Cuts at: 1857 2961 3938 1857
Size: 1104 977 2424
Fragments arranged by size:
2424 1104 977
AgeI A'CCGG_T
Cuts at: 2893 2893
Size: 4505
AleI CACnn'nnGTG
Cuts at: 1475 1475
Size: 4505
AlwNI CAG_nnn'CTG
Cuts at: 1810 3377 1810
Size: 1567 2938
ApaLI G'TGCA_C
Cuts at: 3275 3275
Size: 4505
ApoI r'AATT_y
Cuts at: 399 519 679 1112 1509 3858 4114 399
Size: 120 160 433 397 2349 256 790
Fragments arranged by size:
2349 790 433 397 256 160 120
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 1525 1525
Size: 4505
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 304 337 304
Size: 33 4472
BamHI G'GATC_C
Cuts at: 1530 1530
Size: 4505
BanI G'GyrC_C
Cuts at: 220 1521 1935 2183 2218 220
Size: 1301 414 248 35 2507
Fragments arranged by size:
2507 1301 414 248 35
BanII G_rGCy'C
Cuts at: 581 1362 1519 2549 581
Size: 781 157 1030 2537
Fragments arranged by size:
2537 1030 781 157
BbeI G_GCGC'C
Cuts at: 1939 2187 1939
Size: 248 4257
BbsI GAAGACnn'nnnn_
Cuts at: 353 943 1155 353
Size: 590 212 3703
Fragments arranged by size:
3703 590 212
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3164 4505 2403
Size: 761 1341 2403
Fragments arranged by size:
2403 1341 761
BclI T'GATC_A
Cuts at: 2025 2025
Size: 4505
BfrBI ATG'CAT
Cuts at: 1249 1249
Size: 4505
BglII A'GATC_T
Cuts at: 1417 2020 4057 1417
Size: 603 2037 1865
Fragments arranged by size:
2037 1865 603
BlpI GC'TnA_GC
Cuts at: 1617 1617
Size: 4505
Bme1580I G_kGCm'C
Cuts at: 2130 2223 3279 2130
Size: 93 1056 3356
Fragments arranged by size:
3356 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1717 1798 2133 4277 1717
Size: 81 335 2144 1945
Fragments arranged by size:
2144 1945 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2560 3052 3350 1681
Size: 879 492 298 2836
Fragments arranged by size:
2836 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4209 4209
Size: 4505
BsaAI yAC'GTr
Cuts at: 1497 1858 1497
Size: 361 4144
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 1152 1936 2184 2886 4382 1152
Size: 784 248 702 1496 1275
Fragments arranged by size:
1496 1275 784 702 248
BsaWI w'CCGG_w
Cuts at: 1694 2215 2893 3167 3314 3725 1694
Size: 521 678 274 147 411 2474
Fragments arranged by size:
2474 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 285 315 285
Size: 30 4475
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 611 1316 1364 1445 1478 169
Size: 442 705 48 81 33 3196
Fragments arranged by size:
3196 705 442 81 48 33
BseYI C'CCAG_C
Cuts at: 1930 2688 3265 3761 1930
Size: 758 577 496 2674
Fragments arranged by size:
2674 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2047 2093 3301 104
Size: 1943 46 1208 1308
Fragments arranged by size:
1943 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 581 1266 1362 1470 1519 2297 2487 3279 581
Size: 685 96 108 49 778 190 792 1807
Fragments arranged by size:
1807 792 778 685 190 108 96 49
Bsp1286I G_dGCh'C
Cuts at: 581 1266 1362 1470 1519 2130 2223 2297 2487
Size: 685 96 108 49 611 93 74 190
Cuts at: 2487 2549 3279 581
Size: 62 730 1807
Fragments arranged by size:
1807 730 685 611 190 108 96 93
74 62 49
BspEI T'CCGG_A
Cuts at: 3725 3725
Size: 4505
BspHI T'CATG_A
Cuts at: 3681 3681
Size: 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1555 1769 2071 2452 1555
Size: 214 302 381 3608
Fragments arranged by size:
3608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 156 2797 2851 4335 4500 50
Size: 106 2641 54 1484 165 55
Fragments arranged by size:
2641 1484 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 2417 2417
Size: 4505
BsrFI r'CCGG_y
Cuts at: 2503 2684 2893 2503
Size: 181 209 4115
Fragments arranged by size:
4115 209 181
BsrGI T'GTAC_A
Cuts at: 1288 1288
Size: 4505
BssSI C'ACGA_G
Cuts at: 2776 3134 2776
Size: 358 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 1882 1882
Size: 4505
BstBI TT'CG_AA
Cuts at: 110 2866 110
Size: 2756 1749
BstYI r'GATC_y
Cuts at: 1 146 951 1417 1530 1997 2020 2355 2601
Size: 145 805 466 113 467 23 335 246
Cuts at: 2601 3613 3699 3711 4057 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 805 467 466 449 346 335 246
145 113 86 23 12
BtgI C'CryG_G
Cuts at: 125 1398 1458 125
Size: 1273 60 3172
Fragments arranged by size:
3172 1273 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2455 2636 2799 2455
Size: 181 163 4161
Fragments arranged by size:
4161 181 163
BtsI GCAGTG_nn'
Cuts at: 737 984 1748 737
Size: 247 764 3494
Fragments arranged by size:
3494 764 247
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3069 2211
Size: 858 3647
EaeI y'GGCC_r
Cuts at: 1395 2090 2264 2655 2682 4027 1395
Size: 695 174 391 27 1345 1873
Fragments arranged by size:
1873 1345 695 391 174 27
EagI C'GGCC_G
Cuts at: 2090 2090
Size: 4505
EarI CTCTTCn'nnn_
Cuts at: 410 852 1164 1371 2528 2738 410
Size: 442 312 207 1157 210 2177
Fragments arranged by size:
2177 1157 442 312 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2713 3023 3169 4315 2713
Size: 310 146 1146 2903
Fragments arranged by size:
2903 1146 310 146
EcoICRI GAG'CTC
Cuts at: 579 1360 1517 579
Size: 781 157 3567
Fragments arranged by size:
3567 781 157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2762 3509 3777 2330
Size: 432 747 268 3058
Fragments arranged by size:
3058 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1644 1644
Size: 4505
EcoRI G'AATT_C
Cuts at: 1509 1509
Size: 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 290 981 2524 290
Size: 691 1543 2271
Fragments arranged by size:
2271 1543 691
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 1939 2187 3209 4188 1939
Size: 248 1022 979 2256
Fragments arranged by size:
2256 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 450 482 936 968 2347 2377 2379
Size: 32 254 32 454 32 1379 30 2
Cuts at: 2379 2409 164
Size: 30 2260
Fragments arranged by size:
2260 1379 454 254 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1053 1544 1053
Size: 491 4014
KasI G'GCGC_C
Cuts at: 1935 2183 1935
Size: 248 4257
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 827 886 1185 3176 3360 827
Size: 59 299 1991 184 1972
Fragments arranged by size:
1991 1972 299 184 59
MscI TGG'CCA
Cuts at: 2266 2266
Size: 4505
MslI CAynn'nnrTG
Cuts at: 1046 1475 2494 2621 1046
Size: 429 1019 127 2930
Fragments arranged by size:
2930 1019 429 127
MspA1I CmG'CkG
Cuts at: 1616 1930 2290 3303 3548 1616
Size: 314 360 1013 245 2573
Fragments arranged by size:
2573 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 4505
NarI GG'CG_CC
Cuts at: 1936 2184 1936
Size: 248 4257
NcoI C'CATG_G
Cuts at: 125 125
Size: 4505
NdeI CA'TA_TG
Cuts at: 408 1245 408
Size: 837 3668
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 4505
NsiI A_TGCA'T
Cuts at: 1251 1251
Size: 4505
NspI r_CATG'y
Cuts at: 1558 2058 2965 1558
Size: 500 907 3098
Fragments arranged by size:
3098 907 500
PciI A'CATG_T
Cuts at: 2961 2961
Size: 4505
PfoI T'CCnGG_A
Cuts at: 149 149
Size: 4505
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 974 1006 3644 3676 974
Size: 32 2638 32 1803
Fragments arranged by size:
2638 1803 32 32
PstI C_TGCA'G
Cuts at: 1552 2237 1552
Size: 685 3820
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 1930 2290 1930
Size: 360 4145
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 581 1362 1519 581
Size: 781 157 3567
Fragments arranged by size:
3567 781 157
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 2528 2738 2528
Size: 210 4295
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 438 4022 438
Size: 3584 921
SfcI C'TryA_G
Cuts at: 33 1106 1548 2233 3226 3417 33
Size: 1073 442 685 993 191 1121
Fragments arranged by size:
1121 1073 993 685 442 191
SfoI GGC'GCC
Cuts at: 1937 2185 1937
Size: 248 4257
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 1527 1527
Size: 4505
SmlI C'TyrA_G
Cuts at: 445 1660 2575 3067 3329 445
Size: 1215 915 492 262 1621
Fragments arranged by size:
1621 1215 915 492 262
SnaBI TAC'GTA
Cuts at: 1497 1497
Size: 4505
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 1558 2058 1558
Size: 500 4005
SspI AAT'ATT
Cuts at: 718 718
Size: 4505
StyI C'CwwG_G
Cuts at: 125 852 1639 1993 125
Size: 727 787 354 2637
Fragments arranged by size:
2637 787 727 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 690 1361 2088 2221 2680 2889 690
Size: 671 727 133 459 209 2306
Fragments arranged by size:
2306 727 671 459 209 133
TatI w'GTAC_w
Cuts at: 436 629 1177 1288 4020 436
Size: 193 548 111 2732 921
Fragments arranged by size:
2732 921 548 193 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4022 4054 4022
Size: 32 4473
Tth111I GACn'n_nGTC
Cuts at: 2302 2302
Size: 4505
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2000 2000
Size: 4505
XmaI C'CCGG_G
Cuts at: 1525 1525
Size: 4505
XmnI GAAnn'nnTTC
Cuts at: 1115 1115
Size: 4505
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BbvCI BcgI BglI BmgBI BmtI BpmI Bpu10I
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI
XhoI ZraI