Restriction map for
pF1KSDA1623
(Circular) (Six-base) MAPSORT of: pF1KSDA1623.seq Check: 8934 from: 1 to: 6833
pF1KSDA1623 6833 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 735 1745 735
Size: 1010 5823
AccI GT'mk_AC
Cuts at: 905 1009 3871 905
Size: 104 2862 3867
Fragments arranged by size:
3867 2862 104
Acc65I G'GTAC_C
Cuts at: 3849 3849
Size: 6833
AclI AA'CG_TT
Cuts at: 6673 6673
Size: 6833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1987 4658 5090 5837 6105 1987
Size: 2671 432 747 268 2715
Fragments arranged by size:
2715 2671 747 432 268
AfeI AGC'GCT
Cuts at: 1519 3464 1519
Size: 1945 4888
AflIII A'CryG_T
Cuts at: 436 890 2354 4185 5289 6266 436
Size: 454 1464 1831 1104 977 1003
Fragments arranged by size:
1831 1464 1104 1003 977 454
AgeI A'CCGG_T
Cuts at: 2264 5221 2264
Size: 2957 3876
AleI CACnn'nnGTG
Cuts at: 437 2056 437
Size: 1619 5214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2512 2512
Size: 6833
AlwNI CAG_nnn'CTG
Cuts at: 949 1275 3627 3687 3815 4138 5705 949
Size: 326 2352 60 128 323 1567 2077
Fragments arranged by size:
2352 2077 1567 326 323 128 60
ApaI G_GGCC'C
Cuts at: 2198 2209 3587 3668 2198
Size: 11 1378 81 5363
Fragments arranged by size:
5363 1378 81 11
ApaLI G'TGCA_C
Cuts at: 2487 5603 2487
Size: 3116 3717
ApoI r'AATT_y
Cuts at: 648 3085 3837 6186 6442 648
Size: 2437 752 2349 256 1039
Fragments arranged by size:
2437 2349 1039 752 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6833
AvaI C'yCGr_G
Cuts at: 400 1652 2671 2727 3223 3305 3751 3853 400
Size: 1252 1019 56 496 82 446 102 3380
Fragments arranged by size:
3380 1252 1019 496 446 102 82 56
BamHI G'GATC_C
Cuts at: 3744 3858 3744
Size: 114 6719
BanI G'GyrC_C
Cuts at: 145 303 538 1604 1682 1757 1779 3036 3301
Size: 158 235 1066 78 75 22 1257 265
Cuts at: 3301 3478 3607 3849 4263 4511 4546 145
Size: 177 129 242 414 248 35 2432
Fragments arranged by size:
2432 1257 1066 414 265 248 242 235
177 158 129 78 75 35 22
BanII G_rGCy'C
Cuts at: 407 1659 1961 2050 2124 2147 2198 2209 2641
Size: 1252 302 89 74 23 51 11 432
Cuts at: 2641 2929 3514 3587 3668 3847 4877 407
Size: 288 585 73 81 179 1030 2363
Fragments arranged by size:
2363 1252 1030 585 432 302 288 179
89 81 74 73 51 23 11
BbeI G_GCGC'C
Cuts at: 149 1608 1686 1783 3482 4267 4515 149
Size: 1459 78 97 1699 785 248 2467
Fragments arranged by size:
2467 1699 1459 785 248 97 78
BbsI GAAGACnn'nnnn_
Cuts at: 304 909 1230 304
Size: 605 321 5907
Fragments arranged by size:
5907 605 321
BbvCI CC'TCA_GC
Cuts at: 1762 3199 3416 1762
Size: 1437 217 5179
Fragments arranged by size:
5179 1437 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 247 281 247
Size: 34 6799
BciVI GTATCCnnnnn_n'
Cuts at: 4731 5492 6833 4731
Size: 761 1341 4731
Fragments arranged by size:
4731 1341 761
BclI T'GATC_A
Cuts at: 951 1547 4353 951
Size: 596 2806 3431
Fragments arranged by size:
3431 2806 596
BglI GCCn_nnn'nGGC
Cuts at: 3009 3009
Size: 6833
BglII A'GATC_T
Cuts at: 4348 6385 4348
Size: 2037 4796
BlpI GC'TnA_GC
Cuts at: 1046 1282 1985 2635 3311 3945 1046
Size: 236 703 650 676 634 3934
Fragments arranged by size:
3934 703 676 650 634 236
Bme1580I G_kGCm'C
Cuts at: 157 541 2198 2209 2491 3587 3646 3668 4458
Size: 384 1657 11 282 1096 59 22 790
Cuts at: 4458 4551 5607 157
Size: 93 1056 1383
Fragments arranged by size:
1657 1383 1096 1056 790 384 282 93
59 22 11
BmgBI CAC'GTC
Cuts at: 158 324 893 158
Size: 166 569 6098
Fragments arranged by size:
6098 569 166
BmrI ACTGGGnnnn_n'
Cuts at: 1104 3153 4045 4126 4461 6605 1104
Size: 2049 892 81 335 2144 1332
Fragments arranged by size:
2144 2049 1332 892 335 81
BmtI G_CTAG'C
Cuts at: 1378 1378
Size: 6833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 691 2316 2979 3468 5200 156
Size: 535 1625 663 489 1732 1789
Fragments arranged by size:
1789 1732 1625 663 535 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 715 1432 1549 1841 2056 2092 2347 2779
Size: 531 717 117 292 215 36 255 432
Cuts at: 2779 184
Size: 4238
Fragments arranged by size:
4238 717 531 432 292 255 215 117
36
Bpu10I CC'TnA_GC
Cuts at: 1477 1672 1762 3199 3416 3453 1477
Size: 195 90 1437 217 37 4857
Fragments arranged by size:
4857 1437 217 195 90 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2677 3199 4009 4888 5380 5678 1489
Size: 1188 522 810 879 492 298 2644
Fragments arranged by size:
2644 1188 879 810 522 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2180 3220 3748 6537 2180
Size: 1040 528 2789 2476
Fragments arranged by size:
2789 2476 1040 528
BsaAI yAC'GTr
Cuts at: 467 730 2709 3825 4186 467
Size: 263 1979 1116 361 3114
Fragments arranged by size:
3114 1979 1116 361 263
BsaBI GATnn'nnATC
Cuts at: 6 4347 6384 6
Size: 4341 2037 455
Fragments arranged by size:
4341 2037 455
BsaHI Gr'CG_yC
Cuts at: 146 217 1605 1683 1780 2004 2474 3479 3698
Size: 71 1388 78 97 224 470 1005 219
Cuts at: 3698 4264 4512 5214 6710 146
Size: 566 248 702 1496 269
Fragments arranged by size:
1496 1388 1005 702 566 470 269 248
224 219 97 78 71
BsaWI w'CCGG_w
Cuts at: 220 2264 4022 4543 5221 5495 5642 6053 220
Size: 2044 1758 521 678 274 147 411 1000
Fragments arranged by size:
2044 1758 1000 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3132 3162 3132
Size: 30 6803
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 574 1111 1168 1801 1804 1807 1810 1855 1885
Size: 537 57 633 3 3 3 45 30
Cuts at: 1885 1903 1906 1909 1918 1927 1936 1939 2113
Size: 18 3 3 9 9 9 3 174
Cuts at: 2113 2305 2680 2857 2860 2863 2923 2940 3402
Size: 192 375 177 3 3 60 17 462
Cuts at: 3402 3433 574
Size: 31 3974
Fragments arranged by size:
3974 633 537 462 375 192 177 174
60 57 45 31 30 18 17 9
9 9 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 498 879 909 1623 2306 2732 2804 3361 3680
Size: 381 30 714 683 426 72 557 319
Cuts at: 3680 3759 3778 3784 3808 4258 5016 5593 6089
Size: 79 19 6 24 450 758 577 496
Cuts at: 6089 498
Size: 1242
Fragments arranged by size:
1242 758 714 683 577 557 496 450
426 381 319 79 72 30 24 19
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 2404 820
Size: 1584 5249
BsiEI CG_ry'CG
Cuts at: 104 350 2269 4375 4421 5629 104
Size: 246 1919 2106 46 1208 1308
Fragments arranged by size:
2106 1919 1308 1208 246 46
BsiHKAI G_wGCw'C
Cuts at: 407 2405 2491 2672 2678 2873 3847 4625 4815
Size: 1998 86 181 6 195 974 778 190
Cuts at: 4815 5607 407
Size: 792 1633
Fragments arranged by size:
1998 1633 974 792 778 195 190 181
86 6
BsmI GAATG_Cn'
Cuts at: 395 395
Size: 6833
BsmBI CGTCTCn'nnnn_
Cuts at: 1309 2247 3689 1309
Size: 938 1442 4453
Fragments arranged by size:
4453 1442 938
Bsp1286I G_dGCh'C
Cuts at: 157 407 541 1659 1961 2050 2124 2147 2198
Size: 250 134 1118 302 89 74 23 51
Cuts at: 2198 2209 2405 2491 2641 2672 2678 2873 2929
Size: 11 196 86 150 31 6 195 56
Cuts at: 2929 3514 3587 3646 3668 3847 4458 4551 4625
Size: 585 73 59 22 179 611 93 74
Cuts at: 4625 4815 4877 5607 157
Size: 190 62 730 1383
Fragments arranged by size:
1383 1118 730 611 585 302 250 196
195 190 179 150 134 93 89 86
74 74 73 62 59 56 51 31
23 22 11 6
BspEI T'CCGG_A
Cuts at: 220 6053 220
Size: 5833 1000
BspHI T'CATG_A
Cuts at: 785 3050 6009 785
Size: 2265 2959 1609
Fragments arranged by size:
2959 2265 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 735 1745 3883 4097 4399 4780 735
Size: 1010 2138 214 302 381 2788
Fragments arranged by size:
2788 2138 1010 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1815 5125 5179 6663 6828 50
Size: 1765 3310 54 1484 165 55
Fragments arranged by size:
3310 1765 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4745 4745
Size: 6833
BsrFI r'CCGG_y
Cuts at: 1406 1601 2264 3396 3562 4831 5012 5221 1406
Size: 195 663 1132 166 1269 181 209 3018
Fragments arranged by size:
3018 1269 1132 663 209 195 181 166
BsrGI T'GTAC_A
Cuts at: 932 932
Size: 6833
BssSI C'ACGA_G
Cuts at: 760 2791 5104 5462 760
Size: 2031 2313 358 2131
Fragments arranged by size:
2313 2131 2031 358
BstAPI GCAn_nnn'nTGC
Cuts at: 651 4210 651
Size: 3559 3274
BstBI TT'CG_AA
Cuts at: 110 5194 110
Size: 5084 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2058 2058
Size: 6833
BstYI r'GATC_y
Cuts at: 1 2462 3744 3858 4325 4348 4683 4929 5941
Size: 2461 1282 114 467 23 335 246 1012
Cuts at: 5941 6027 6039 6385 1
Size: 86 12 346 449
Fragments arranged by size:
2461 1282 1012 467 449 346 335 246
114 86 23 12
BstZ17I GTA'TAC
Cuts at: 906 906
Size: 6833
Bsu36I CC'TnA_GG
Cuts at: 1322 1322
Size: 6833
BtgI C'CryG_G
Cuts at: 125 543 1091 1614 2558 2997 3473 3656 125
Size: 418 548 523 944 439 476 183 3302
Fragments arranged by size:
3302 944 548 523 476 439 418 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2794 4783 4964 5127 2794
Size: 1989 181 163 4500
Fragments arranged by size:
4500 1989 181 163
BtsI GCAGTG_nn'
Cuts at: 521 2984 3073 3372 3580 4076 521
Size: 2463 89 299 208 496 3278
Fragments arranged by size:
3278 2463 496 299 208 89
DraI TTT'AAA
Cuts at: 3832 3832
Size: 6833
DraIII CAC_nnn'GTG
Cuts at: 684 3652 684
Size: 2968 3865
DrdI GACnn_nn'nnGTC
Cuts at: 4539 5397 4539
Size: 858 5975
EaeI y'GGCC_r
Cuts at: 128 1535 2093 2150 2511 2532 2598 2711 2738
Size: 1407 558 57 361 21 66 113 27
Cuts at: 2738 2750 2901 2994 3346 3629 3659 4418 4592
Size: 12 151 93 352 283 30 759 174
Cuts at: 4592 4983 5010 6355 128
Size: 391 27 1345 606
Fragments arranged by size:
1407 1345 759 606 558 391 361 352
283 174 151 113 93 66 57 30
27 27 21 12
EagI C'GGCC_G
Cuts at: 4418 4418
Size: 6833
EarI CTCTTCn'nnn_
Cuts at: 997 1192 1489 1904 2410 2629 3292 3437 4856
Size: 195 297 415 506 219 663 145 1419
Cuts at: 4856 5066 997
Size: 210 2764
Fragments arranged by size:
2764 1419 663 506 415 297 219 210
195 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 409 1729 2128 2152 2403 5041 5351 5497 6643
Size: 1320 399 24 251 2638 310 146 1146
Cuts at: 6643 409
Size: 599
Fragments arranged by size:
2638 1320 1146 599 399 310 251 146
24
EcoICRI GAG'CTC
Cuts at: 405 3845 405
Size: 3440 3393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 715 1432 1549 1841 1987 2056 2092 2347
Size: 531 717 117 292 146 69 36 255
Cuts at: 2347 2779 4658 5090 5837 6105 184
Size: 432 1879 432 747 268 912
Fragments arranged by size:
1879 912 747 717 531 432 432 292
268 255 146 117 69 36
EcoNI CCTnn'n_nnAGG
Cuts at: 1321 2035 2095 3415 1321
Size: 714 60 1320 4739
Fragments arranged by size:
4739 1320 714 60
EcoO109I rG'GnC_Cy
Cuts at: 185 1581 1941 2028 2194 2205 3026 3356 3408
Size: 1396 360 87 166 11 821 330 52
Cuts at: 3408 3584 3624 3665 3972 185
Size: 176 40 41 307 3046
Fragments arranged by size:
3046 1396 821 360 330 307 176 166
87 52 41 40 11
EcoRI G'AATT_C
Cuts at: 3837 3837
Size: 6833
EcoRV GAT'ATC
Cuts at: 2724 2724
Size: 6833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1995 4852 1995
Size: 2857 3976
FspI TGC'GCA
Cuts at: 508 4614 508
Size: 4106 2727
HaeII r_GCGC'y
Cuts at: 149 1421 1521 1608 1686 1783 1807 2434 2622
Size: 1272 100 87 78 97 24 627 188
Cuts at: 2622 2772 2799 2862 3021 3466 3482 3766 4267
Size: 150 27 63 159 445 16 284 501
Cuts at: 4267 4515 5537 6516 149
Size: 248 1022 979 466
Fragments arranged by size:
1272 1022 979 627 501 466 445 284
248 188 159 150 100 97 87 78
63 27 24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3304 3336 4675 4705 4707 4737 3304
Size: 32 1339 30 2 30 5400
Fragments arranged by size:
5400 1339 32 30 30 2
HincII GTy'rAC
Cuts at: 1010 1467 2448 2583 3872 1010
Size: 457 981 135 1289 3971
Fragments arranged by size:
3971 1289 981 457 135
HindIII A'AGCT_T
Cuts at: 235 235
Size: 6833
KasI G'GCGC_C
Cuts at: 145 1604 1682 1779 3478 4263 4511 145
Size: 1459 78 97 1699 785 248 2467
Fragments arranged by size:
2467 1699 1459 785 248 97 78
KpnI G_GTAC'C
Cuts at: 3853 3853
Size: 6833
MluI A'CGCG_T
Cuts at: 436 6266 436
Size: 5830 1003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2252 3347 5504 5688 2252
Size: 1095 2157 184 3397
Fragments arranged by size:
3397 2157 1095 184
MscI TGG'CCA
Cuts at: 1537 2713 2752 2996 4594 1537
Size: 1176 39 244 1598 3776
Fragments arranged by size:
3776 1598 1176 244 39
MslI CAynn'nnrTG
Cuts at: 388 437 1059 2056 2707 4822 4949 388
Size: 49 622 997 651 2115 127 2272
Fragments arranged by size:
2272 2115 997 651 622 127 49
MspA1I CmG'CkG
Cuts at: 545 913 1194 1266 1616 1739 1766 2502 2613
Size: 368 281 72 350 123 27 736 111
Cuts at: 2613 3537 3634 3812 3944 4258 4618 5631 5876
Size: 924 97 178 132 314 360 1013 245
Cuts at: 5876 545
Size: 1502
Fragments arranged by size:
1502 1013 924 736 368 360 350 314
281 245 178 132 123 111 97 72
27
NaeI GCC'GGC
Cuts at: 3564 5014 3564
Size: 1450 5383
NarI GG'CG_CC
Cuts at: 146 1605 1683 1780 3479 4264 4512 146
Size: 1459 78 97 1699 785 248 2467
Fragments arranged by size:
2467 1699 1459 785 248 97 78
NcoI C'CATG_G
Cuts at: 125 2558 3656 125
Size: 2433 1098 3302
Fragments arranged by size:
3302 2433 1098
NgoMIV G'CCGG_C
Cuts at: 3562 5012 3562
Size: 1450 5383
NheI G'CTAG_C
Cuts at: 1374 1374
Size: 6833
NspI r_CATG'y
Cuts at: 3886 4386 5293 3886
Size: 500 907 5426
Fragments arranged by size:
5426 907 500
PasI CC'CwG_GG
Cuts at: 3022 3587 3022
Size: 565 6268
PciI A'CATG_T
Cuts at: 5289 5289
Size: 6833
PflMI CCAn_nnn'nTGG
Cuts at: 2520 3627 3815 2520
Size: 1107 188 5538
Fragments arranged by size:
5538 1107 188
PfoI T'CCnGG_A
Cuts at: 692 713 1946 2033 2360 692
Size: 21 1233 87 327 5165
Fragments arranged by size:
5165 1233 327 87 21
PmeI GTTT'AAAC
Cuts at: 3832 3832
Size: 6833
PmlI CAC'GTG
Cuts at: 730 2709 730
Size: 1979 4854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2373 5972 6004 2341
Size: 32 3599 32 3170
Fragments arranged by size:
3599 3170 32 32
PpuMI rG'GwC_Cy
Cuts at: 1581 3026 3356 1581
Size: 1445 330 5058
Fragments arranged by size:
5058 1445 330
PspOMI G'GGCC_C
Cuts at: 2194 2205 3583 3664 2194
Size: 11 1378 81 5363
Fragments arranged by size:
5363 1378 81 11
PspXI vC'TCGA_Gb
Cuts at: 2671 2671
Size: 6833
PstI C_TGCA'G
Cuts at: 210 260 647 1271 1589 2501 2618 3080 3126
Size: 50 387 624 318 912 117 462 46
Cuts at: 3126 3880 4565 210
Size: 754 685 2478
Fragments arranged by size:
2478 912 754 685 624 462 387 318
117 50 46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6833
PvuII CAG'CTG
Cuts at: 913 1194 1266 2613 4258 4618 913
Size: 281 72 1347 1645 360 3128
Fragments arranged by size:
3128 1645 1347 360 281 72
RsrII CG'GwC_CG
Cuts at: 5028 5028
Size: 6833
SacI G_AGCT'C
Cuts at: 407 3847 407
Size: 3440 3393
SacII CC_GC'GG
Cuts at: 546 1617 546
Size: 1071 5762
SalI G'TCGA_C
Cuts at: 1008 3870 1008
Size: 2862 3971
SanDI GG'GwC_CC
Cuts at: 3026 3356 3026
Size: 330 6503
SapI GCTCTTCn'nnn_
Cuts at: 1192 2410 3292 4856 5066 1192
Size: 1218 882 1564 210 2959
Fragments arranged by size:
2959 1564 1218 882 210
SbfI CC_TGCA'GG
Cuts at: 260 3880 260
Size: 3620 3213
ScaI AGT'ACT
Cuts at: 6350 6350
Size: 6833
SexAI A'CCwGG_T
Cuts at: 272 272
Size: 6833
SfcI C'TryA_G
Cuts at: 33 206 256 308 643 1267 1585 2497 2614
Size: 173 50 52 335 624 318 912 117
Cuts at: 2614 3076 3122 3876 4561 5554 5745 33
Size: 462 46 754 685 993 191 1121
Fragments arranged by size:
1121 993 912 754 685 624 462 335
318 191 173 117 52 50 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 3009 3009
Size: 6833
SfoI GGC'GCC
Cuts at: 147 1606 1684 1781 3480 4265 4513 147
Size: 1459 78 97 1699 785 248 2467
Fragments arranged by size:
2467 1699 1459 785 248 97 78
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6833
SmaI CCC'GGG
Cuts at: 2729 3307 3855 2729
Size: 578 548 5707
Fragments arranged by size:
5707 578 548
SmlI C'TyrA_G
Cuts at: 1504 2671 2692 3214 3751 3988 4903 5395 5657
Size: 1167 21 522 537 237 915 492 262
Cuts at: 5657 1504
Size: 2680
Fragments arranged by size:
2680 1167 915 537 522 492 262 237
21
SnaBI TAC'GTA
Cuts at: 3825 3825
Size: 6833
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6833
SphI G_CATG'C
Cuts at: 3886 4386 3886
Size: 500 6333
StuI AGG'CCT
Cuts at: 2779 2962 2779
Size: 183 6650
StyI C'CwwG_G
Cuts at: 125 2558 2969 2975 3656 3967 4321 125
Size: 2433 411 6 681 311 354 2637
Fragments arranged by size:
2637 2433 681 411 354 311 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 786 2329 2551 2724 3054 3180 4416 4549
Size: 422 1543 222 173 330 126 1236 133
Cuts at: 4549 5008 5217 364
Size: 459 209 1980
Fragments arranged by size:
1980 1543 1236 459 422 330 222 209
173 133 126
TatI w'GTAC_w
Cuts at: 866 932 6348 866
Size: 66 5416 1351
Fragments arranged by size:
5416 1351 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3132 3164 3729 3761 6350 6382 3132
Size: 32 565 32 2589 32 3583
Fragments arranged by size:
3583 2589 565 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4630 4630
Size: 6833
XbaI T'CTAG_A
Cuts at: 825 3864 825
Size: 3039 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2658 3111 4328 2658
Size: 453 1217 5163
Fragments arranged by size:
5163 1217 453
XhoI C'TCGA_G
Cuts at: 2671 3751 2671
Size: 1080 5753
XmaI C'CCGG_G
Cuts at: 2727 3305 3853 2727
Size: 578 548 5707
Fragments arranged by size:
5707 578 548
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BsiWI BssHII BstEII ClaI CspCI FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PshAI PsiI
PsrI SgrAI SrfI SspI SwaI XmnI ZraI