Restriction map for
pF1KSDA1563
(Circular) (Six-base) MAPSORT of: pF1KSDA1563.seq Check: 8151 from: 1 to: 8108
pF1KSDA1563 8108 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1813 1813
Size: 8108
AatII G_ACGT'C
Cuts at: 3559 3559
Size: 8108
AccI GT'mk_AC
Cuts at: 311 2862 3467 5146 311
Size: 2551 605 1679 3273
Fragments arranged by size:
3273 2551 1679 605
Acc65I G'GTAC_C
Cuts at: 649 5124 649
Size: 4475 3633
AclI AA'CG_TT
Cuts at: 7948 7948
Size: 8108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 820 1399 2461 3041 4448 5005 5933 6365
Size: 503 579 1062 580 1407 557 928 432
Cuts at: 6365 7112 7380 317
Size: 747 268 1045
Fragments arranged by size:
1407 1062 1045 928 747 580 579 557
503 432 268
AflII C'TTAA_G
Cuts at: 1611 4381 4840 1611
Size: 2770 459 4879
Fragments arranged by size:
4879 2770 459
AflIII A'CryG_T
Cuts at: 464 5460 6564 7541 464
Size: 4996 1104 977 1031
Fragments arranged by size:
4996 1104 1031 977
AgeI A'CCGG_T
Cuts at: 652 6496 652
Size: 5844 2264
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 8108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4214 4363 4214
Size: 149 7959
AlwNI CAG_nnn'CTG
Cuts at: 798 2727 5413 6980 798
Size: 1929 2686 1567 1926
Fragments arranged by size:
2686 1929 1926 1567
ApaI G_GGCC'C
Cuts at: 4051 4051
Size: 8108
ApaLI G'TGCA_C
Cuts at: 6878 6878
Size: 8108
ApoI r'AATT_y
Cuts at: 477 1112 2298 2496 3796 4177 4388 4708 4971
Size: 635 1186 198 1300 381 211 320 263
Cuts at: 4971 5112 7461 7717 477
Size: 141 2349 256 868
Fragments arranged by size:
2349 1300 1186 868 635 381 320 263
256 211 198 141
AseI AT'TA_AT
Cuts at: 20 20
Size: 8108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8108
AvaI C'yCGr_G
Cuts at: 5128 5128
Size: 8108
AvrII C'CTAG_G
Cuts at: 1762 1762
Size: 8108
BamHI G'GATC_C
Cuts at: 163 202 3246 5133 163
Size: 39 3044 1887 3138
Fragments arranged by size:
3138 3044 1887 39
BanI G'GyrC_C
Cuts at: 649 1366 4028 4112 4198 5124 5538 5786 5821
Size: 717 2662 84 86 926 414 248 35
Cuts at: 5821 649
Size: 2936
Fragments arranged by size:
2936 2662 926 717 414 248 86 84
35
BanII G_rGCy'C
Cuts at: 1428 2892 4051 5122 6152 1428
Size: 1464 1159 1071 1030 3384
Fragments arranged by size:
3384 1464 1159 1071 1030
BbeI G_GCGC'C
Cuts at: 4032 4116 5542 5790 4032
Size: 84 1426 248 6350
Fragments arranged by size:
6350 1426 248 84
BbsI GAAGACnn'nnnn_
Cuts at: 869 1572 2018 2821 3422 3526 4634 4907 869
Size: 703 446 803 601 104 1108 273 4070
Fragments arranged by size:
4070 1108 803 703 601 446 273 104
BbvCI CC'TCA_GC
Cuts at: 1293 4785 1293
Size: 3492 4616
BciVI GTATCCnnnnn_n'
Cuts at: 1998 6006 6767 8108 1998
Size: 4008 761 1341 1998
Fragments arranged by size:
4008 1998 1341 761
BclI T'GATC_A
Cuts at: 1017 5628 1017
Size: 4611 3497
BglI GCCn_nnn'nGGC
Cuts at: 4037 4037
Size: 8108
BglII A'GATC_T
Cuts at: 4868 5623 7660 4868
Size: 755 2037 5316
Fragments arranged by size:
5316 2037 755
BlpI GC'TnA_GC
Cuts at: 765 2531 5220 765
Size: 1766 2689 3653
Fragments arranged by size:
3653 2689 1766
Bme1580I G_kGCm'C
Cuts at: 1748 2308 4051 5733 5826 6882 1748
Size: 560 1743 1682 93 1056 2974
Fragments arranged by size:
2974 1743 1682 1056 560 93
BmrI ACTGGGnnnn_n'
Cuts at: 682 2812 2958 3696 4045 4499 4647 5320 5401
Size: 2130 146 738 349 454 148 673 81
Cuts at: 5401 5736 7880 682
Size: 335 2144 910
Fragments arranged by size:
2144 2130 910 738 673 454 349 335
148 146 81
BmtI G_CTAG'C
Cuts at: 2345 2345
Size: 8108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4887 6475 4887
Size: 1588 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 1442 1837 1983 2084 2696 354
Size: 1088 395 146 101 612 5766
Fragments arranged by size:
5766 1088 612 395 146 101
Bpu10I CC'TnA_GC
Cuts at: 1293 1427 4418 4785 1293
Size: 134 2991 367 4616
Fragments arranged by size:
4616 2991 367 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2865 5284 6163 6655 6953 2865
Size: 2419 879 492 298 4020
Fragments arranged by size:
4020 2419 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 673 1320 7812 673
Size: 647 6492 969
Fragments arranged by size:
6492 969 647
BsaAI yAC'GTr
Cuts at: 4320 5100 5461 4320
Size: 780 361 6967
Fragments arranged by size:
6967 780 361
BsaBI GATnn'nnATC
Cuts at: 6 3898 4512 4616 5622 7659 6
Size: 3892 614 104 1006 2037 455
Fragments arranged by size:
3892 2037 1006 614 455 104
BsaHI Gr'CG_yC
Cuts at: 3556 4029 4113 4888 5539 5787 6489 7985 3556
Size: 473 84 775 651 248 702 1496 3679
Fragments arranged by size:
3679 1496 775 702 651 473 248 84
BsaWI w'CCGG_w
Cuts at: 652 5297 5818 6496 6770 6917 7328 652
Size: 4645 521 678 274 147 411 1432
Fragments arranged by size:
4645 1432 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 767 797 1559 1589 4357 4387 767
Size: 30 762 30 2768 30 4488
Fragments arranged by size:
4488 2768 762 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 3079 4250 4448 4879 616
Size: 2463 1171 198 431 3845
Fragments arranged by size:
3845 2463 1171 431 198
BseYI C'CCAG_C
Cuts at: 236 721 1740 2908 5533 6291 6868 7364 236
Size: 485 1019 1168 2625 758 577 496 980
Fragments arranged by size:
2625 1168 1019 980 758 577 496 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 849 1607 4249 4720 515
Size: 334 758 2642 471 3903
Fragments arranged by size:
3903 2642 758 471 334
BsiEI CG_ry'CG
Cuts at: 104 5650 5696 6904 104
Size: 5546 46 1208 1308
Fragments arranged by size:
5546 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 608 1056 1428 5122 5900 6090 6882 608
Size: 448 372 3694 778 190 792 1834
Fragments arranged by size:
3694 1834 792 778 448 372 190
BsmI GAATG_Cn'
Cuts at: 1047 1902 2445 1047
Size: 855 543 6710
Fragments arranged by size:
6710 855 543
Bsp1286I G_dGCh'C
Cuts at: 608 1056 1428 1748 2308 2892 4051 5122 5733
Size: 448 372 320 560 584 1159 1071 611
Cuts at: 5733 5826 5900 6090 6152 6882 608
Size: 93 74 190 62 730 1834
Fragments arranged by size:
1834 1159 1071 730 611 584 560 448
372 320 190 93 74 62
BspEI T'CCGG_A
Cuts at: 7328 7328
Size: 8108
BspHI T'CATG_A
Cuts at: 7284 7284
Size: 8108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1813 4331 5158 5372 5674 6055 1813
Size: 2518 827 214 302 381 3866
Fragments arranged by size:
3866 2518 827 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6400 6454 7938 8103 50
Size: 6350 54 1484 165 55
Fragments arranged by size:
6350 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 539 3410 6020 539
Size: 2871 2610 2627
Fragments arranged by size:
2871 2627 2610
BsrFI r'CCGG_y
Cuts at: 652 6106 6287 6496 652
Size: 5454 181 209 2264
Fragments arranged by size:
5454 2264 209 181
BsrGI T'GTAC_A
Cuts at: 774 774
Size: 8108
BssSI C'ACGA_G
Cuts at: 2212 6379 6737 2212
Size: 4167 358 3583
Fragments arranged by size:
4167 3583 358
BstAPI GCAn_nnn'nTGC
Cuts at: 516 827 1051 1849 5485 516
Size: 311 224 798 3636 3139
Fragments arranged by size:
3636 3139 798 311 224
BstBI TT'CG_AA
Cuts at: 110 6469 110
Size: 6359 1749
BstEII G'GTnAC_C
Cuts at: 1828 3696 1828
Size: 1868 6240
BstXI CCAn_nnnn'nTGG
Cuts at: 2430 2430
Size: 8108
BstYI r'GATC_y
Cuts at: 1 163 202 795 3246 3886 4868 5001 5133
Size: 162 39 593 2451 640 982 133 132
Cuts at: 5133 5600 5623 5958 6204 7216 7302 7314 7660
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7660 1
Size: 449
Fragments arranged by size:
2451 1012 982 640 593 467 449 346
335 246 162 133 132 86 39 23
12
Bsu36I CC'TnA_GG
Cuts at: 3062 3062
Size: 8108
BtgI C'CryG_G
Cuts at: 125 4916 125
Size: 4791 3317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1085 1766 6058 6239 6402 1085
Size: 681 4292 181 163 2791
Fragments arranged by size:
4292 2791 681 181 163
BtsI GCAGTG_nn'
Cuts at: 446 677 944 1953 3195 3228 3989 4307 5351
Size: 231 267 1009 1242 33 761 318 1044
Cuts at: 5351 446
Size: 3203
Fragments arranged by size:
3203 1242 1044 1009 761 318 267 231
33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1508 1543 1508
Size: 35 8073
DraI TTT'AAA
Cuts at: 5107 5107
Size: 8108
DraIII CAC_nnn'GTG
Cuts at: 3002 3223 3002
Size: 221 7887
DrdI GACnn_nn'nnGTC
Cuts at: 454 5814 6672 454
Size: 5360 858 1890
Fragments arranged by size:
5360 1890 858
EaeI y'GGCC_r
Cuts at: 486 2040 2993 3582 4714 5693 5867 6258 6285
Size: 1554 953 589 1132 979 174 391 27
Cuts at: 6285 7630 486
Size: 1345 964
Fragments arranged by size:
1554 1345 1132 979 964 953 589 391
174 27
EagI C'GGCC_G
Cuts at: 5693 5693
Size: 8108
EarI CTCTTCn'nnn_
Cuts at: 133 332 2288 2461 3567 6131 6341 133
Size: 199 1956 173 1106 2564 210 1900
Fragments arranged by size:
2564 1956 1900 1106 210 199 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6316 6626 6772 7918 6316
Size: 310 146 1146 6506
Fragments arranged by size:
6506 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1426 5120 1426
Size: 3694 4414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 354 820 1399 1442 1837 1983 2084 2461
Size: 37 466 579 43 395 146 101 377
Cuts at: 2461 2696 3041 4448 5005 5933 6365 7112 7380
Size: 235 345 1407 557 928 432 747 268
Cuts at: 7380 317
Size: 1045
Fragments arranged by size:
1407 1045 928 747 579 557 466 432
395 377 345 268 235 146 101 43
37
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 4430 4996 2057
Size: 2373 566 5169
Fragments arranged by size:
5169 2373 566
EcoO109I rG'GnC_Cy
Cuts at: 2049 4047 5247 2049
Size: 1998 1200 4910
Fragments arranged by size:
4910 1998 1200
EcoRI G'AATT_C
Cuts at: 477 4177 5112 477
Size: 3700 935 3473
Fragments arranged by size:
3700 3473 935
EcoRV GAT'ATC
Cuts at: 1533 2259 3630 1533
Size: 726 1371 6011
Fragments arranged by size:
6011 1371 726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4496 6127 4496
Size: 1631 6477
FspI TGC'GCA
Cuts at: 5889 5889
Size: 8108
HaeII r_GCGC'y
Cuts at: 4032 4116 5542 5790 6812 7791 4032
Size: 84 1426 248 1022 979 4349
Fragments arranged by size:
4349 1426 1022 979 248 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 167 199 906 938 1063 1095 1559 1591 2248
Size: 32 707 32 125 32 464 32 657
Cuts at: 2248 2280 3001 3033 3147 3179 3602 3634 4092
Size: 32 721 32 114 32 423 32 458
Cuts at: 4092 4124 4357 4389 4492 4524 5950 5980 5982
Size: 32 233 32 103 32 1426 30 2
Cuts at: 5982 6012 167
Size: 30 2263
Fragments arranged by size:
2263 1426 721 707 657 464 458 423
233 125 114 103 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1894 2863 5147 1894
Size: 969 2284 4855
Fragments arranged by size:
4855 2284 969
HindIII A'AGCT_T
Cuts at: 423 2122 2683 5089 423
Size: 1699 561 2406 3442
Fragments arranged by size:
3442 2406 1699 561
KasI G'GCGC_C
Cuts at: 4028 4112 5538 5786 4028
Size: 84 1426 248 6350
Fragments arranged by size:
6350 1426 248 84
KpnI G_GTAC'C
Cuts at: 653 5128 653
Size: 4475 3633
MluI A'CGCG_T
Cuts at: 7541 7541
Size: 8108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1939 6779 6963 1939
Size: 4840 184 3084
Fragments arranged by size:
4840 3084 184
MscI TGG'CCA
Cuts at: 488 2995 3584 4716 5869 488
Size: 2507 589 1132 1153 2727
Fragments arranged by size:
2727 2507 1153 1132 589
MslI CAynn'nnrTG
Cuts at: 1751 3527 4196 6097 6224 1751
Size: 1776 669 1901 127 3635
Fragments arranged by size:
3635 1901 1776 669 127
MspA1I CmG'CkG
Cuts at: 1740 2325 3991 4398 4590 5219 5533 5893 6906
Size: 585 1666 407 192 629 314 360 1013
Cuts at: 6906 7151 1740
Size: 245 2697
Fragments arranged by size:
2697 1666 1013 629 585 407 360 314
245 192
NaeI GCC'GGC
Cuts at: 6289 6289
Size: 8108
NarI GG'CG_CC
Cuts at: 4029 4113 5539 5787 4029
Size: 84 1426 248 6350
Fragments arranged by size:
6350 1426 248 84
NcoI C'CATG_G
Cuts at: 125 4916 125
Size: 4791 3317
NgoMIV G'CCGG_C
Cuts at: 6287 6287
Size: 8108
NheI G'CTAG_C
Cuts at: 2341 2341
Size: 8108
NruI TCG'CGA
Cuts at: 4622 4622
Size: 8108
NspI r_CATG'y
Cuts at: 468 2908 3351 4195 5066 5161 5661 6568 468
Size: 2440 443 844 871 95 500 907 2008
Fragments arranged by size:
2440 2008 907 871 844 500 443 95
PasI CC'CwG_GG
Cuts at: 4272 4272
Size: 8108
PciI A'CATG_T
Cuts at: 464 6564 464
Size: 6100 2008
PflMI CCAn_nnn'nTGG
Cuts at: 3002 3426 3002
Size: 424 7684
PmeI GTTT'AAAC
Cuts at: 5107 5107
Size: 8108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3087 7247 7279 3055
Size: 32 4160 32 3884
Fragments arranged by size:
4160 3884 32 32
PpuMI rG'GwC_Cy
Cuts at: 2049 2049
Size: 8108
PspOMI G'GGCC_C
Cuts at: 4047 4047
Size: 8108
PstI C_TGCA'G
Cuts at: 1977 2732 2903 4805 5155 5840 1977
Size: 755 171 1902 350 685 4245
Fragments arranged by size:
4245 1902 755 685 350 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8108
PvuII CAG'CTG
Cuts at: 1740 2325 3991 4398 5533 5893 1740
Size: 585 1666 407 1135 360 3955
Fragments arranged by size:
3955 1666 1135 585 407 360
RsrII CG'GwC_CG
Cuts at: 6303 6303
Size: 8108
SacI G_AGCT'C
Cuts at: 1428 5122 1428
Size: 3694 4414
SalI G'TCGA_C
Cuts at: 2861 5145 2861
Size: 2284 5824
SapI GCTCTTCn'nnn_
Cuts at: 6131 6341 6131
Size: 210 7898
SbfI CC_TGCA'GG
Cuts at: 5155 5155
Size: 8108
ScaI AGT'ACT
Cuts at: 2105 7625 2105
Size: 5520 2588
SfcI C'TryA_G
Cuts at: 33 312 1973 2054 2115 2225 2315 2728 2899
Size: 279 1661 81 61 110 90 413 171
Cuts at: 2899 3468 4801 5151 5836 6829 7020 33
Size: 569 1333 350 685 993 191 1121
Fragments arranged by size:
1661 1333 1121 993 685 569 413 350
279 191 171 110 90 81 61
SfoI GGC'GCC
Cuts at: 4030 4114 5540 5788 4030
Size: 84 1426 248 6350
Fragments arranged by size:
6350 1426 248 84
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8108
SmaI CCC'GGG
Cuts at: 5130 5130
Size: 8108
SmlI C'TyrA_G
Cuts at: 1611 2844 4381 4840 5263 6178 6670 6932 1611
Size: 1233 1537 459 423 915 492 262 2787
Fragments arranged by size:
2787 1537 1233 915 492 459 423 262
SnaBI TAC'GTA
Cuts at: 5100 5100
Size: 8108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8108
SphI G_CATG'C
Cuts at: 2908 5161 5661 2908
Size: 2253 500 5355
Fragments arranged by size:
5355 2253 500
SspI AAT'ATT
Cuts at: 4086 4237 4086
Size: 151 7957
StuI AGG'CCT
Cuts at: 179 2280 4249 179
Size: 2101 1969 4038
Fragments arranged by size:
4038 2101 1969
StyI C'CwwG_G
Cuts at: 125 167 1762 2171 4056 4734 4916 5242 5596
Size: 42 1595 409 1885 678 182 326 354
Cuts at: 5596 125
Size: 2637
Fragments arranged by size:
2637 1885 1595 678 409 354 326 182
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1165 3225 5691 5824 6283 6492 1165
Size: 2060 2466 133 459 209 2781
Fragments arranged by size:
2781 2466 2060 459 209 133
TatI w'GTAC_w
Cuts at: 774 1402 2103 7623 774
Size: 628 701 5520 1259
Fragments arranged by size:
5520 1259 701 628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3804 3836 7625 7657 3804
Size: 32 3789 32 4255
Fragments arranged by size:
4255 3789 32 32
Tth111I GACn'n_nGTC
Cuts at: 5905 5905
Size: 8108
XbaI T'CTAG_A
Cuts at: 375 5139 375
Size: 4764 3344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5603 5603
Size: 8108
XmaI C'CCGG_G
Cuts at: 5128 5128
Size: 8108
XmnI GAAnn'nnTTC
Cuts at: 1256 2818 2929 4181 1256
Size: 1562 111 1252 5183
Fragments arranged by size:
5183 1562 1252 111
ZraI GAC'GTC
Cuts at: 3557 3557
Size: 8108
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AleI AloI AscI BaeI BcgI BfrBI BmgBI
BsiWI BsmBI BssHII BstZ17I ClaI FseI FspAI HpaI
MfeI NdeI NotI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI