Restriction map for
pF1KSDA1559
(Circular) (Six-base) MAPSORT of: pF1KSDA1559.seq Check: 253 from: 1 to: 4736
pF1KSDA1559 4736 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1774 1774
Size: 4736
Acc65I G'GTAC_C
Cuts at: 1752 1752
Size: 4736
AclI AA'CG_TT
Cuts at: 4576 4576
Size: 4736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 2561 2993 3740 4008 451
Size: 2110 432 747 268 1179
Fragments arranged by size:
2110 1179 747 432 268
AflIII A'CryG_T
Cuts at: 2088 3192 4169 2088
Size: 1104 977 2655
Fragments arranged by size:
2655 1104 977
AgeI A'CCGG_T
Cuts at: 793 3124 793
Size: 2331 2405
AleI CACnn'nnGTG
Cuts at: 794 962 1466 1550 794
Size: 168 504 84 3980
Fragments arranged by size:
3980 504 168 84
AlwNI CAG_nnn'CTG
Cuts at: 936 2041 3608 936
Size: 1105 1567 2064
Fragments arranged by size:
2064 1567 1105
ApaLI G'TGCA_C
Cuts at: 761 3506 761
Size: 2745 1991
ApoI r'AATT_y
Cuts at: 187 701 953 1121 1625 1721 1740 4089 4345
Size: 514 252 168 504 96 19 2349 256
Cuts at: 4345 187
Size: 578
Fragments arranged by size:
2349 578 514 504 256 252 168 96
19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4736
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
AvaI C'yCGr_G
Cuts at: 1756 1756
Size: 4736
BamHI G'GATC_C
Cuts at: 195 1761 195
Size: 1566 3170
BanI G'GyrC_C
Cuts at: 1752 2166 2414 2449 1752
Size: 414 248 35 4039
Fragments arranged by size:
4039 414 248 35
BanII G_rGCy'C
Cuts at: 1750 2780 1750
Size: 1030 3706
BbeI G_GCGC'C
Cuts at: 2170 2418 2170
Size: 248 4488
BbsI GAAGACnn'nnnn_
Cuts at: 667 1255 1339 667
Size: 588 84 4064
Fragments arranged by size:
4064 588 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 922 956 922
Size: 34 4702
BciVI GTATCCnnnnn_n'
Cuts at: 2634 3395 4736 2634
Size: 761 1341 2634
Fragments arranged by size:
2634 1341 761
BclI T'GATC_A
Cuts at: 2256 2256
Size: 4736
BglII A'GATC_T
Cuts at: 2251 4288 2251
Size: 2037 2699
BlpI GC'TnA_GC
Cuts at: 1848 1848
Size: 4736
Bme1580I G_kGCm'C
Cuts at: 765 2361 2454 3510 765
Size: 1596 93 1056 1991
Fragments arranged by size:
1991 1596 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1948 2029 2364 4508 1948
Size: 81 335 2144 2176
Fragments arranged by size:
2176 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 4736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 380
Size: 4736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1912 2791 3283 3581 1912
Size: 879 492 298 3067
Fragments arranged by size:
3067 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1383 4440 1383
Size: 3057 1679
BsaAI yAC'GTr
Cuts at: 1728 2089 1728
Size: 361 4375
BsaBI GATnn'nnATC
Cuts at: 6 2250 4287 6
Size: 2244 2037 455
Fragments arranged by size:
2244 2037 455
BsaHI Gr'CG_yC
Cuts at: 2167 2415 3117 4613 2167
Size: 248 702 1496 2290
Fragments arranged by size:
2290 1496 702 248
BsaWI w'CCGG_w
Cuts at: 793 1925 2446 3124 3398 3545 3956 793
Size: 1132 521 678 274 147 411 1573
Fragments arranged by size:
1573 1132 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 378 408 620 650 378
Size: 30 212 30 4464
Fragments arranged by size:
4464 212 30 30
BseYI C'CCAG_C
Cuts at: 2161 2919 3496 3992 2161
Size: 758 577 496 2905
Fragments arranged by size:
2905 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2278 2324 3532 104
Size: 2174 46 1208 1308
Fragments arranged by size:
2174 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 765 845 1750 2528 2718 3510 765
Size: 80 905 778 190 792 1991
Fragments arranged by size:
1991 905 792 778 190 80
BsmI GAATG_Cn'
Cuts at: 502 1373 1683 502
Size: 871 310 3555
Fragments arranged by size:
3555 871 310
Bsp1286I G_dGCh'C
Cuts at: 765 845 1750 2361 2454 2528 2718 2780 3510
Size: 80 905 611 93 74 190 62 730
Cuts at: 3510 765
Size: 1991
Fragments arranged by size:
1991 905 730 611 190 93 80 74
62
BspEI T'CCGG_A
Cuts at: 3956 3956
Size: 4736
BspHI T'CATG_A
Cuts at: 3912 3912
Size: 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1786 2000 2302 2683 1786
Size: 214 302 381 3839
Fragments arranged by size:
3839 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3028 3082 4566 4731 50
Size: 2978 54 1484 165 55
Fragments arranged by size:
2978 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2648 2648
Size: 4736
BsrFI r'CCGG_y
Cuts at: 793 2734 2915 3124 793
Size: 1941 181 209 2405
Fragments arranged by size:
2405 1941 209 181
BsrGI T'GTAC_A
Cuts at: 1012 1012
Size: 4736
BssSI C'ACGA_G
Cuts at: 1298 3007 3365 1298
Size: 1709 358 2669
Fragments arranged by size:
2669 1709 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2113 2113
Size: 4736
BstBI TT'CG_AA
Cuts at: 110 3097 110
Size: 2987 1749
BstYI r'GATC_y
Cuts at: 1 195 1761 2228 2251 2586 2832 3844 3930
Size: 194 1566 467 23 335 246 1012 86
Cuts at: 3930 3942 4288 1
Size: 12 346 449
Fragments arranged by size:
1566 1012 467 449 346 335 246 194
86 23 12
BtgI C'CryG_G
Cuts at: 125 132 125
Size: 7 4729
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2686 2867 3030 2686
Size: 181 163 4392
Fragments arranged by size:
4392 181 163
BtsI GCAGTG_nn'
Cuts at: 1979 1979
Size: 4736
DraI TTT'AAA
Cuts at: 1735 1735
Size: 4736
DrdI GACnn_nn'nnGTC
Cuts at: 2442 3300 2442
Size: 858 3878
EaeI y'GGCC_r
Cuts at: 158 2321 2495 2886 2913 4258 158
Size: 2163 174 391 27 1345 636
Fragments arranged by size:
2163 1345 636 391 174 27
EagI C'GGCC_G
Cuts at: 2321 2321
Size: 4736
EarI CTCTTCn'nnn_
Cuts at: 1400 2759 2969 1400
Size: 1359 210 3167
Fragments arranged by size:
3167 1359 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2944 3254 3400 4546 2944
Size: 310 146 1146 3134
Fragments arranged by size:
3134 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1748 1748
Size: 4736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 451 2561 2993 3740 4008 380
Size: 71 2110 432 747 268 1108
Fragments arranged by size:
2110 1108 747 432 268 71
EcoO109I rG'GnC_Cy
Cuts at: 262 1875 262
Size: 1613 3123
EcoRI G'AATT_C
Cuts at: 187 701 953 1625 1740 187
Size: 514 252 672 115 3183
Fragments arranged by size:
3183 672 514 252 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1689 2755 1689
Size: 1066 3670
FspI TGC'GCA
Cuts at: 2517 2517
Size: 4736
HaeII r_GCGC'y
Cuts at: 2170 2418 3440 4419 2170
Size: 248 1022 979 2487
Fragments arranged by size:
2487 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 2578 2608 2610 2640 352
Size: 32 2194 30 2 30 2448
Fragments arranged by size:
2448 2194 32 30 30 2
HincII GTy'rAC
Cuts at: 691 1775 691
Size: 1084 3652
KasI G'GCGC_C
Cuts at: 2166 2414 2166
Size: 248 4488
KpnI G_GTAC'C
Cuts at: 1756 1756
Size: 4736
MluI A'CGCG_T
Cuts at: 4169 4169
Size: 4736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1309 3407 3591 1309
Size: 2098 184 2454
Fragments arranged by size:
2454 2098 184
MscI TGG'CCA
Cuts at: 160 2497 160
Size: 2337 2399
MslI CAynn'nnrTG
Cuts at: 429 710 794 878 962 1130 1214 1298 1382
Size: 281 84 84 84 168 84 84 84
Cuts at: 1382 1466 1550 1634 2725 2852 429
Size: 84 84 84 1091 127 2313
Fragments arranged by size:
2313 1091 281 168 127 84 84 84
84 84 84 84 84 84
MspA1I CmG'CkG
Cuts at: 1847 2161 2521 3534 3779 1847
Size: 314 360 1013 245 2804
Fragments arranged by size:
2804 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2917 2917
Size: 4736
NarI GG'CG_CC
Cuts at: 2167 2415 2167
Size: 248 4488
NcoI C'CATG_G
Cuts at: 125 132 125
Size: 7 4729
NgoMIV G'CCGG_C
Cuts at: 2915 2915
Size: 4736
NspI r_CATG'y
Cuts at: 1789 2289 3196 1789
Size: 500 907 3329
Fragments arranged by size:
3329 907 500
PciI A'CATG_T
Cuts at: 3192 3192
Size: 4736
PfoI T'CCnGG_A
Cuts at: 191 191
Size: 4736
PmeI GTTT'AAAC
Cuts at: 1735 1735
Size: 4736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4704
PpuMI rG'GwC_Cy
Cuts at: 262 262
Size: 4736
PsiI TTA'TAA
Cuts at: 725 725
Size: 4736
PstI C_TGCA'G
Cuts at: 1783 2468 1783
Size: 685 4051
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4736
PvuII CAG'CTG
Cuts at: 2161 2521 2161
Size: 360 4376
RsrII CG'GwC_CG
Cuts at: 2931 2931
Size: 4736
SacI G_AGCT'C
Cuts at: 1750 1750
Size: 4736
SalI G'TCGA_C
Cuts at: 1773 1773
Size: 4736
SapI GCTCTTCn'nnn_
Cuts at: 2759 2969 2759
Size: 210 4526
SbfI CC_TGCA'GG
Cuts at: 1783 1783
Size: 4736
ScaI AGT'ACT
Cuts at: 4253 4253
Size: 4736
SfcI C'TryA_G
Cuts at: 33 240 459 1779 2464 3457 3648 33
Size: 207 219 1320 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 219 207 191
SfoI GGC'GCC
Cuts at: 2168 2416 2168
Size: 248 4488
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
SgrAI Cr'CCGG_yG
Cuts at: 793 793
Size: 4736
SmaI CCC'GGG
Cuts at: 1758 1758
Size: 4736
SmlI C'TyrA_G
Cuts at: 1891 2806 3298 3560 1891
Size: 915 492 262 3067
Fragments arranged by size:
3067 915 492 262
SnaBI TAC'GTA
Cuts at: 1728 1728
Size: 4736
SpeI A'CTAG_T
Cuts at: 67 1272 67
Size: 1205 3531
SphI G_CATG'C
Cuts at: 1789 2289 1789
Size: 500 4236
StuI AGG'CCT
Cuts at: 748 832 916 1420 1672 748
Size: 84 84 504 252 3812
Fragments arranged by size:
3812 504 252 84 84
StyI C'CwwG_G
Cuts at: 125 132 1870 2224 125
Size: 7 1738 354 2637
Fragments arranged by size:
2637 1738 354 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2319 2452 2911 3120 2319
Size: 133 459 209 3935
Fragments arranged by size:
3935 459 209 133
TatI w'GTAC_w
Cuts at: 924 1012 1110 4251 924
Size: 88 98 3141 1409
Fragments arranged by size:
3141 1409 98 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 620 652 4253 4285 214
Size: 32 374 32 3601 32 665
Fragments arranged by size:
3601 665 374 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2533 2533
Size: 4736
XbaI T'CTAG_A
Cuts at: 1767 1767
Size: 4736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2231
Size: 4736
XmaI C'CCGG_G
Cuts at: 1756 1756
Size: 4736
XmnI GAAnn'nnTTC
Cuts at: 1084 1084
Size: 4736
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SrfI SspI SwaI
XhoI ZraI