Restriction map for
pF1KSDA1540
(Circular) (Six-base) MAPSORT of: pF1KSDA1540.seq Check: 5485 from: 1 to: 4406
pF1KSDA1540 4406 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1147 1147
Size: 4406
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1164 1251 2231 2663 3410 3678 1164
Size: 87 980 432 747 268 1892
Fragments arranged by size:
1892 980 747 432 268 87
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 983 983
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 1139 1711 3278 1139
Size: 572 1567 2267
Fragments arranged by size:
2267 1567 572
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 1410 3759 4015 1410
Size: 2349 256 1801
Fragments arranged by size:
2349 1801 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1426 1426
Size: 4406
BamHI G'GATC_C
Cuts at: 1431 1431
Size: 4406
BanI G'GyrC_C
Cuts at: 1422 1836 2084 2119 1422
Size: 414 248 35 3709
Fragments arranged by size:
3709 414 248 35
BanII G_rGCy'C
Cuts at: 182 1308 1420 2450 182
Size: 1126 112 1030 2138
Fragments arranged by size:
2138 1126 1030 112
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BbsI GAAGACnn'nnnn_
Cuts at: 366 997 1360 366
Size: 631 363 3412
Fragments arranged by size:
3412 631 363
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 482 1926 482
Size: 1444 2962
BglII A'GATC_T
Cuts at: 1921 3958 1921
Size: 2037 2369
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 233 2031 2124 3180 233
Size: 1798 93 1056 1459
Fragments arranged by size:
1798 1459 1056 93
BmgBI CAC'GTC
Cuts at: 594 594
Size: 4406
BmrI ACTGGGnnnn_n'
Cuts at: 570 1249 1618 1699 2034 4178 570
Size: 679 369 81 335 2144 798
Fragments arranged by size:
2144 798 679 369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 1327
Size: 4406
Bpu10I CC'TnA_GC
Cuts at: 812 812
Size: 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2461 2953 3251 1582
Size: 879 492 298 2737
Fragments arranged by size:
2737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1398 1759 1398
Size: 361 4045
BsaBI GATnn'nnATC
Cuts at: 6 670 1920 3957 6
Size: 664 1250 2037 455
Fragments arranged by size:
2037 1250 664 455
BsaHI Gr'CG_yC
Cuts at: 1837 2085 2787 4283 1837
Size: 248 702 1496 1960
Fragments arranged by size:
1960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1595 2116 2794 3068 3215 3626 1595
Size: 521 678 274 147 411 2375
Fragments arranged by size:
2375 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 256 286 256
Size: 30 4376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 871 1054 145
Size: 726 183 3497
Fragments arranged by size:
3497 726 183
BseYI C'CCAG_C
Cuts at: 1831 2589 3166 3662 1831
Size: 758 577 496 2575
Fragments arranged by size:
2575 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 574
Size: 4406
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1420 2198 2388 3180 1420
Size: 778 190 792 2646
Fragments arranged by size:
2646 792 778 190
BsmI GAATG_Cn'
Cuts at: 434 434
Size: 4406
BsmBI CGTCTCn'nnnn_
Cuts at: 600 600
Size: 4406
Bsp1286I G_dGCh'C
Cuts at: 182 233 1308 1420 2031 2124 2198 2388 2450
Size: 51 1075 112 611 93 74 190 62
Cuts at: 2450 3180 182
Size: 730 1408
Fragments arranged by size:
1408 1075 730 611 190 112 93 74
62 51
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1456 1670 1972 2353 1147
Size: 309 214 302 381 3200
Fragments arranged by size:
3200 381 309 302 214
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 617 2318 617
Size: 1701 2705
BsrFI r'CCGG_y
Cuts at: 2404 2585 2794 2404
Size: 181 209 4016
Fragments arranged by size:
4016 209 181
BssSI C'ACGA_G
Cuts at: 184 905 2677 3035 184
Size: 721 1772 358 1555
Fragments arranged by size:
1772 1555 721 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1139 1783 1139
Size: 644 3762
BstBI TT'CG_AA
Cuts at: 110 2767 110
Size: 2657 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 624 624
Size: 4406
BstYI r'GATC_y
Cuts at: 1 665 1431 1898 1921 2256 2502 3514 3600
Size: 664 766 467 23 335 246 1012 86
Cuts at: 3600 3612 3958 1
Size: 12 346 449
Fragments arranged by size:
1012 766 664 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1232 1232
Size: 4406
BtgI C'CryG_G
Cuts at: 125 329 710 125
Size: 204 381 3821
Fragments arranged by size:
3821 381 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 678 959 2356 2537 2700 678
Size: 281 1397 181 163 2384
Fragments arranged by size:
2384 1397 281 181 163
BtsI GCAGTG_nn'
Cuts at: 586 1649 586
Size: 1063 3343
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 1362 2112 2970 1362
Size: 750 858 2798
Fragments arranged by size:
2798 858 750
EaeI y'GGCC_r
Cuts at: 224 437 848 911 1296 1991 2165 2556 2583
Size: 213 411 63 385 695 174 391 27
Cuts at: 2583 3928 224
Size: 1345 702
Fragments arranged by size:
1345 702 695 411 391 385 213 174
63 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 454 832 2429 2639 454
Size: 378 1597 210 2221
Fragments arranged by size:
2221 1597 378 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 2924 3070 4216 2614
Size: 310 146 1146 2804
Fragments arranged by size:
2804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1164 1251 1327 2231 2663 3410 3678 1164
Size: 87 76 904 432 747 268 1892
Fragments arranged by size:
1892 904 747 432 268 87 76
EcoO109I rG'GnC_Cy
Cuts at: 1545 1545
Size: 4406
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 306 314 1840 2088 3110 4089 306
Size: 8 1526 248 1022 979 623
Fragments arranged by size:
1526 1022 979 623 248 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1197 1229 1351 1383 2248 2278 2280 2310 1197
Size: 32 122 32 865 30 2 30 3293
Fragments arranged by size:
3293 865 122 32 32 30 30 2
HincII GTy'rAC
Cuts at: 459 1445 459
Size: 986 3420
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 3077 3261 299
Size: 2778 184 1444
Fragments arranged by size:
2778 1444 184
MscI TGG'CCA
Cuts at: 850 913 1298 2167 850
Size: 63 385 869 3089
Fragments arranged by size:
3089 869 385 63
MslI CAynn'nnrTG
Cuts at: 622 892 1000 2395 2522 622
Size: 270 108 1395 127 2506
Fragments arranged by size:
2506 1395 270 127 108
MspA1I CmG'CkG
Cuts at: 820 1517 1831 2191 3204 3449 820
Size: 697 314 360 1013 245 1777
Fragments arranged by size:
1777 1013 697 360 314 245
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NcoI C'CATG_G
Cuts at: 125 710 125
Size: 585 3821
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NspI r_CATG'y
Cuts at: 555 739 1212 1459 1959 2866 555
Size: 184 473 247 500 907 2095
Fragments arranged by size:
2095 907 500 473 247 184
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PstI C_TGCA'G
Cuts at: 1453 2138 1453
Size: 685 3721
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 820 1831 2191 820
Size: 1011 360 3035
Fragments arranged by size:
3035 1011 360
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 580 964 3923 580
Size: 384 2959 1063
Fragments arranged by size:
2959 1063 384
SexAI A'CCwGG_T
Cuts at: 635 635
Size: 4406
SfcI C'TryA_G
Cuts at: 33 1449 2134 3127 3318 33
Size: 1416 685 993 191 1121
Fragments arranged by size:
1416 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 1561 2476 2968 3230 1561
Size: 915 492 262 2737
Fragments arranged by size:
2737 915 492 262
SnaBI TAC'GTA
Cuts at: 1398 1398
Size: 4406
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 125 161 710 1540 1894 125
Size: 36 549 830 354 2637
Fragments arranged by size:
2637 830 549 354 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1989 2122 2581 2790 1989
Size: 133 459 209 3605
Fragments arranged by size:
3605 459 209 133
TatI w'GTAC_w
Cuts at: 578 962 3921 578
Size: 384 2959 1063
Fragments arranged by size:
2959 1063 384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4374
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1345 1901 1345
Size: 556 3850
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI