Restriction map for
pF1KSDA1518
(Circular) (Six-base) MAPSORT of: pF1KSDA1518.seq Check: 2087 from: 1 to: 5690
pF1KSDA1518 5690 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 195 729 195
Size: 534 5156
AatII G_ACGT'C
Cuts at: 373 373
Size: 5690
AccI GT'mk_AC
Cuts at: 2728 2728
Size: 5690
Acc65I G'GTAC_C
Cuts at: 2706 2706
Size: 5690
AclI AA'CG_TT
Cuts at: 5530 5530
Size: 5690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2278 2284 3515 3947 4694 4962 2278
Size: 6 1231 432 747 268 3006
Fragments arranged by size:
3006 1231 747 432 268 6
AflII C'TTAA_G
Cuts at: 814 814
Size: 5690
AflIII A'CryG_T
Cuts at: 306 3042 4146 5123 306
Size: 2736 1104 977 873
Fragments arranged by size:
2736 1104 977 873
AgeI A'CCGG_T
Cuts at: 654 4078 654
Size: 3424 2266
AleI CACnn'nnGTG
Cuts at: 655 1990 655
Size: 1335 4355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 164 164
Size: 5690
AlwNI CAG_nnn'CTG
Cuts at: 138 1032 1165 1194 2181 2995 4562 138
Size: 894 133 29 987 814 1567 1266
Fragments arranged by size:
1567 1266 987 894 814 133 29
ApaI G_GGCC'C
Cuts at: 1188 1224 1550 1188
Size: 36 326 5328
Fragments arranged by size:
5328 326 36
ApaLI G'TGCA_C
Cuts at: 4460 4460
Size: 5690
ApoI r'AATT_y
Cuts at: 2694 5043 5299 2694
Size: 2349 256 3085
Fragments arranged by size:
3085 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 529 1413 1820 2038 2710 529
Size: 884 407 218 672 3509
Fragments arranged by size:
3509 884 672 407 218
BamHI G'GATC_C
Cuts at: 2715 2715
Size: 5690
BanI G'GyrC_C
Cuts at: 657 674 706 928 1168 1537 2054 2706 3120
Size: 17 32 222 240 369 517 652 414
Cuts at: 3120 3368 3403 657
Size: 248 35 2944
Fragments arranged by size:
2944 652 517 414 369 248 240 222
35 32 17
BanII G_rGCy'C
Cuts at: 340 565 869 1188 1224 1252 1481 1496 1550
Size: 225 304 319 36 28 229 15 54
Cuts at: 1550 2656 2704 3734 340
Size: 1106 48 1030 2296
Fragments arranged by size:
2296 1106 1030 319 304 229 225 54
48 36 28 15
BbeI G_GCGC'C
Cuts at: 1172 3124 3372 1172
Size: 1952 248 3490
Fragments arranged by size:
3490 1952 248
BbsI GAAGACnn'nnnn_
Cuts at: 1394 2272 1394
Size: 878 4812
BbvCI CC'TCA_GC
Cuts at: 1216 1847 1216
Size: 631 5059
BciVI GTATCCnnnnn_n'
Cuts at: 1101 3588 4349 5690 1101
Size: 2487 761 1341 1101
Fragments arranged by size:
2487 1341 1101 761
BclI T'GATC_A
Cuts at: 3210 3210
Size: 5690
BglI GCCn_nnn'nGGC
Cuts at: 1054 1239 1500 1921 2335 2401 2410 1054
Size: 185 261 421 414 66 9 4334
Fragments arranged by size:
4334 421 414 261 185 66 9
BglII A'GATC_T
Cuts at: 3205 5242 3205
Size: 2037 3653
BlpI GC'TnA_GC
Cuts at: 1894 2802 1894
Size: 908 4782
Bme1580I G_kGCm'C
Cuts at: 677 711 931 1188 1224 1294 1301 1469 1540
Size: 34 220 257 36 70 7 168 71
Cuts at: 1540 1550 2510 3315 3408 4464 677
Size: 10 960 805 93 1056 1903
Fragments arranged by size:
1903 1056 960 805 257 220 168 93
71 70 36 34 10 7
BmrI ACTGGGnnnn_n'
Cuts at: 569 659 895 1288 2902 2983 3318 5462 569
Size: 90 236 393 1614 81 335 2144 797
Fragments arranged by size:
2144 1614 797 393 335 236 90 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1446 1704 2567 4057 1446
Size: 258 863 1490 3079
Fragments arranged by size:
3079 1490 863 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 745 751 766 931 1021 1216 1606 1653
Size: 258 6 15 165 90 195 390 47
Cuts at: 1653 487
Size: 4524
Fragments arranged by size:
4524 390 258 195 165 90 47 15
6
Bpu10I CC'TnA_GC
Cuts at: 334 1043 1216 1765 1847 334
Size: 709 173 549 82 4177
Fragments arranged by size:
4177 709 549 173 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 997 2866 3745 4237 4535 250
Size: 747 1869 879 492 298 1405
Fragments arranged by size:
1869 1405 879 747 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 223 463 907 997 5394 223
Size: 240 444 90 4397 519
Fragments arranged by size:
4397 519 444 240 90
BsaAI yAC'GTr
Cuts at: 144 273 687 2100 2682 3043 144
Size: 129 414 1413 582 361 2791
Fragments arranged by size:
2791 1413 582 414 361 129
BsaBI GATnn'nnATC
Cuts at: 6 1342 3204 5241 6
Size: 1336 1862 2037 455
Fragments arranged by size:
2037 1862 1336 455
BsaHI Gr'CG_yC
Cuts at: 370 1169 3121 3369 4071 5567 370
Size: 799 1952 248 702 1496 493
Fragments arranged by size:
1952 1496 799 702 493 248
BsaWI w'CCGG_w
Cuts at: 654 1610 2879 3400 4078 4352 4499 4910 654
Size: 956 1269 521 678 274 147 411 1434
Fragments arranged by size:
1434 1269 956 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1273 1303 1273
Size: 30 5660
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 748 1540 1600 1629 1888 1891 748
Size: 792 60 29 259 3 4547
Fragments arranged by size:
4547 792 259 60 29 3
BseYI C'CCAG_C
Cuts at: 174 1007 1064 1760 2509 3115 3873 4450 4946
Size: 833 57 696 749 606 758 577 496
Cuts at: 4946 174
Size: 918
Fragments arranged by size:
918 833 758 749 696 606 577 496
57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 337 2122 2194 124
Size: 213 1785 72 3620
Fragments arranged by size:
3620 1785 213 72
BsiEI CG_ry'CG
Cuts at: 104 438 584 3232 3278 4486 104
Size: 334 146 2648 46 1208 1308
Fragments arranged by size:
2648 1308 1208 334 146 46
BsiHKAI G_wGCw'C
Cuts at: 340 391 465 761 782 869 1456 2471 2548
Size: 51 74 296 21 87 587 1015 77
Cuts at: 2548 2626 2704 3482 3672 4464 340
Size: 78 78 778 190 792 1566
Fragments arranged by size:
1566 1015 792 778 587 296 190 87
78 78 77 74 51 21
BsmBI CGTCTCn'nnnn_
Cuts at: 532 532
Size: 5690
Bsp1286I G_dGCh'C
Cuts at: 340 391 465 565 677 711 761 782 869
Size: 51 74 100 112 34 50 21 87
Cuts at: 869 931 1188 1224 1252 1294 1301 1456 1469
Size: 62 257 36 28 42 7 155 13
Cuts at: 1469 1481 1496 1540 1550 2471 2510 2548 2626
Size: 12 15 44 10 921 39 38 78
Cuts at: 2626 2656 2704 3315 3408 3482 3672 3734 4464
Size: 30 48 611 93 74 190 62 730
Cuts at: 4464 340
Size: 1566
Fragments arranged by size:
1566 921 730 611 257 190 155 112
100 93 87 78 74 74 62 62
51 50 48 44 42 39 38 36
34 30 28 21 15 13 12 10
7
BspEI T'CCGG_A
Cuts at: 4910 4910
Size: 5690
BspHI T'CATG_A
Cuts at: 1571 4866 1571
Size: 3295 2395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 195 729 2740 2954 3256 3637 195
Size: 534 2011 214 302 381 2248
Fragments arranged by size:
2248 2011 534 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1965 3982 4036 5520 5685 50
Size: 1915 2017 54 1484 165 55
Fragments arranged by size:
2017 1915 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1235 3602 1235
Size: 2367 3323
BsrFI r'CCGG_y
Cuts at: 553 583 654 1540 1799 3688 3869 4078 553
Size: 30 71 886 259 1889 181 209 2165
Fragments arranged by size:
2165 1889 886 259 209 181 71 30
BssSI C'ACGA_G
Cuts at: 3961 4319 3961
Size: 358 5332
BstAPI GCAn_nnn'nTGC
Cuts at: 1032 2181 3067 1032
Size: 1149 886 3655
Fragments arranged by size:
3655 1149 886
BstBI TT'CG_AA
Cuts at: 110 4051 110
Size: 3941 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 558 558
Size: 5690
BstYI r'GATC_y
Cuts at: 1 2715 3182 3205 3540 3786 4798 4884 4896
Size: 2714 467 23 335 246 1012 86 12
Cuts at: 4896 5242 1
Size: 346 449
Fragments arranged by size:
2714 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 224 348 438 474 490 631 1132 1601
Size: 99 124 90 36 16 141 501 469
Cuts at: 1601 2168 2370 2444 125
Size: 567 202 74 3371
Fragments arranged by size:
3371 567 501 469 202 141 124 99
90 74 36 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1376 2094 2552 3640 3821 3984 1376
Size: 718 458 1088 181 163 3082
Fragments arranged by size:
3082 1088 718 458 181 163
BtsI GCAGTG_nn'
Cuts at: 320 1841 2933 320
Size: 1521 1092 3077
Fragments arranged by size:
3077 1521 1092
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 381 416 381
Size: 35 5655
DraI TTT'AAA
Cuts at: 2689 2689
Size: 5690
DrdI GACnn_nn'nnGTC
Cuts at: 1096 2101 2194 3396 4254 1096
Size: 1005 93 1202 858 2532
Fragments arranged by size:
2532 1202 1005 858 93
EaeI y'GGCC_r
Cuts at: 435 542 581 1078 1151 1748 1787 2255 2360
Size: 107 39 497 73 597 39 468 105
Cuts at: 2360 2472 2501 3275 3449 3840 3867 5212 435
Size: 112 29 774 174 391 27 1345 913
Fragments arranged by size:
1345 913 774 597 497 468 391 174
112 107 105 73 39 39 29 27
EagI C'GGCC_G
Cuts at: 435 581 3275 435
Size: 146 2694 2850
Fragments arranged by size:
2850 2694 146
EarI CTCTTCn'nnn_
Cuts at: 1444 2305 2669 3713 3923 1444
Size: 861 364 1044 210 3211
Fragments arranged by size:
3211 1044 861 364 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3898 4208 4354 5500 3898
Size: 310 146 1146 4088
Fragments arranged by size:
4088 1146 310 146
EcoICRI GAG'CTC
Cuts at: 338 867 2702 338
Size: 529 1835 3326
Fragments arranged by size:
3326 1835 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 745 751 766 931 1021 1216 1606 1653
Size: 258 6 15 165 90 195 390 47
Cuts at: 1653 2278 2284 3515 3947 4694 4962 487
Size: 625 6 1231 432 747 268 1215
Fragments arranged by size:
1231 1215 747 625 432 390 268 258
195 165 90 47 15 6 6
EcoO109I rG'GnC_Cy
Cuts at: 135 164 204 897 1220 1221 1547 1716 2829
Size: 29 40 693 323 1 326 169 1113
Cuts at: 2829 135
Size: 2996
Fragments arranged by size:
2996 1113 693 326 323 169 40 29
1
EcoRI G'AATT_C
Cuts at: 2694 2694
Size: 5690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3709 3709
Size: 5690
FspI TGC'GCA
Cuts at: 3471 3471
Size: 5690
HaeII r_GCGC'y
Cuts at: 324 1024 1172 3124 3372 4394 5373 324
Size: 700 148 1952 248 1022 979 641
Fragments arranged by size:
1952 1022 979 700 641 248 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1642 1674 1840 1872 1894 1926 2425 2457 2575
Size: 32 166 32 22 32 499 32 118
Cuts at: 2575 2607 3532 3562 3564 3594 1642
Size: 32 925 30 2 30 3738
Fragments arranged by size:
3738 925 499 166 118 32 32 32
32 32 30 30 22 2
HincII GTy'rAC
Cuts at: 1638 2106 2421 2729 1638
Size: 468 315 308 4599
Fragments arranged by size:
4599 468 315 308
KasI G'GCGC_C
Cuts at: 1168 3120 3368 1168
Size: 1952 248 3490
Fragments arranged by size:
3490 1952 248
KpnI G_GTAC'C
Cuts at: 2710 2710
Size: 5690
MluI A'CGCG_T
Cuts at: 306 5123 306
Size: 4817 873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4361 4545 4361
Size: 184 5506
MscI TGG'CCA
Cuts at: 1080 1153 2257 3451 1080
Size: 73 1104 1194 3319
Fragments arranged by size:
3319 1194 1104 73
MslI CAynn'nnrTG
Cuts at: 655 1990 3679 3806 655
Size: 1335 1689 127 2539
Fragments arranged by size:
2539 1689 1335 127
MspA1I CmG'CkG
Cuts at: 476 492 729 847 1165 1182 1303 1786 1815
Size: 16 237 118 318 17 121 483 29
Cuts at: 1815 2181 2192 2513 2801 3115 3475 4488 4733
Size: 366 11 321 288 314 360 1013 245
Cuts at: 4733 476
Size: 1433
Fragments arranged by size:
1433 1013 483 366 360 321 318 314
288 245 237 121 118 29 17 16
11
NaeI GCC'GGC
Cuts at: 585 1801 3871 585
Size: 1216 2070 2404
Fragments arranged by size:
2404 2070 1216
NarI GG'CG_CC
Cuts at: 1169 3121 3369 1169
Size: 1952 248 3490
Fragments arranged by size:
3490 1952 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5690
NdeI CA'TA_TG
Cuts at: 1325 1325
Size: 5690
NgoMIV G'CCGG_C
Cuts at: 583 1799 3869 583
Size: 1216 2070 2404
Fragments arranged by size:
2404 2070 1216
NruI TCG'CGA
Cuts at: 2537 2537
Size: 5690
NspI r_CATG'y
Cuts at: 960 2743 3243 4150 960
Size: 1783 500 907 2500
Fragments arranged by size:
2500 1783 907 500
PasI CC'CwG_GG
Cuts at: 160 160
Size: 5690
PciI A'CATG_T
Cuts at: 4146 4146
Size: 5690
PflMI CCAn_nnn'nTGG
Cuts at: 1194 1275 1629 1194
Size: 81 354 5255
Fragments arranged by size:
5255 354 81
PfoI T'CCnGG_A
Cuts at: 782 782
Size: 5690
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PmlI CAC'GTG
Cuts at: 144 687 144
Size: 543 5147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4829 4861 4829
Size: 32 5658
PpuMI rG'GwC_Cy
Cuts at: 135 164 204 897 135
Size: 29 40 693 4928
Fragments arranged by size:
4928 693 40 29
PspOMI G'GGCC_C
Cuts at: 1184 1220 1546 1184
Size: 36 326 5328
Fragments arranged by size:
5328 326 36
PstI C_TGCA'G
Cuts at: 1028 1037 1308 1776 2000 2737 3422 1028
Size: 9 271 468 224 737 685 3296
Fragments arranged by size:
3296 737 685 468 271 224 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5690
PvuII CAG'CTG
Cuts at: 729 1815 2181 2513 3115 3475 729
Size: 1086 366 332 602 360 2944
Fragments arranged by size:
2944 1086 602 366 360 332
RsrII CG'GwC_CG
Cuts at: 1098 3885 1098
Size: 2787 2903
SacI G_AGCT'C
Cuts at: 340 869 2704 340
Size: 529 1835 3326
Fragments arranged by size:
3326 1835 529
SacII CC_GC'GG
Cuts at: 477 493 477
Size: 16 5674
SalI G'TCGA_C
Cuts at: 2727 2727
Size: 5690
SanDI GG'GwC_CC
Cuts at: 164 164
Size: 5690
SapI GCTCTTCn'nnn_
Cuts at: 1444 2305 3713 3923 1444
Size: 861 1408 210 3211
Fragments arranged by size:
3211 1408 861 210
SbfI CC_TGCA'GG
Cuts at: 1776 2737 1776
Size: 961 4729
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 2057 2057
Size: 5690
SfcI C'TryA_G
Cuts at: 33 1024 1033 1304 1772 1996 2229 2733 3418
Size: 991 9 271 468 224 233 504 685
Cuts at: 3418 4411 4602 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 991 685 504 468 271 233
224 191 9
SfoI GGC'GCC
Cuts at: 1170 3122 3370 1170
Size: 1952 248 3490
Fragments arranged by size:
3490 1952 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SgrAI Cr'CCGG_yG
Cuts at: 654 1540 654
Size: 886 4804
SmaI CCC'GGG
Cuts at: 1415 2712 1415
Size: 1297 4393
SmlI C'TyrA_G
Cuts at: 265 529 814 1012 1820 2845 3760 4252 4514
Size: 264 285 198 808 1025 915 492 262
Cuts at: 4514 265
Size: 1441
Fragments arranged by size:
1441 1025 915 808 492 285 264 262
198
SnaBI TAC'GTA
Cuts at: 2682 2682
Size: 5690
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 960 2743 3243 960
Size: 1783 500 3407
Fragments arranged by size:
3407 1783 500
StuI AGG'CCT
Cuts at: 1075 1242 1376 1770 1075
Size: 167 134 394 4995
Fragments arranged by size:
4995 394 167 134
StyI C'CwwG_G
Cuts at: 125 200 986 2564 2824 3178 125
Size: 75 786 1578 260 354 2637
Fragments arranged by size:
2637 1578 786 354 260 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 564 1486 1498 3273 3406 3865 4074 564
Size: 922 12 1775 133 459 209 2180
Fragments arranged by size:
2180 1775 922 459 209 133 12
TatI w'GTAC_w
Cuts at: 807 5205 807
Size: 4398 1292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 176 208 1244 1273 1276 1305 5207 5239 176
Size: 32 1036 29 3 29 3902 32 627
Fragments arranged by size:
3902 1036 627 32 32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 2386 3487 2386
Size: 1101 4589
XbaI T'CTAG_A
Cuts at: 2721 2721
Size: 5690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2166 3185 2166
Size: 1019 4671
XhoI C'TCGA_G
Cuts at: 529 1820 529
Size: 1291 4399
XmaI C'CCGG_G
Cuts at: 1413 2710 1413
Size: 1297 4393
ZraI GAC'GTC
Cuts at: 371 371
Size: 5690
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI AvrII BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NsiI PacI PshAI PsiI
PspXI PsrI SfiI SrfI SspI SwaI XmnI