Restriction map for
pF1KSDA1499
(Circular) (Six-base) MAPSORT of: pF1KSDA1499.seq Check: 7277 from: 1 to: 4988
pF1KSDA1499 4988 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1902 1902
Size: 4988
AatII G_ACGT'C
Cuts at: 400 400
Size: 4988
AccI GT'mk_AC
Cuts at: 1412 2026 1412
Size: 614 4374
Acc65I G'GTAC_C
Cuts at: 1093 1277 2004 1093
Size: 184 727 4077
Fragments arranged by size:
4077 727 184
AclI AA'CG_TT
Cuts at: 4828 4828
Size: 4988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1025 2813 3245 3992 4260 950
Size: 75 1788 432 747 268 1678
Fragments arranged by size:
1788 1678 747 432 268 75
AflIII A'CryG_T
Cuts at: 608 1184 2340 3444 4421 608
Size: 576 1156 1104 977 1175
Fragments arranged by size:
1175 1156 1104 977 576
AgeI A'CCGG_T
Cuts at: 3376 3376
Size: 4988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1720 1916 1720
Size: 196 4792
AlwNI CAG_nnn'CTG
Cuts at: 2293 3860 2293
Size: 1567 3421
ApaI G_GGCC'C
Cuts at: 380 380
Size: 4988
ApaLI G'TGCA_C
Cuts at: 541 3758 541
Size: 3217 1771
ApoI r'AATT_y
Cuts at: 1992 4341 4597 1992
Size: 2349 256 2383
Fragments arranged by size:
2383 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4988
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
AvaI C'yCGr_G
Cuts at: 363 589 2008 363
Size: 226 1419 3343
Fragments arranged by size:
3343 1419 226
BamHI G'GATC_C
Cuts at: 993 1216 1500 2013 993
Size: 223 284 513 3968
Fragments arranged by size:
3968 513 284 223
BanI G'GyrC_C
Cuts at: 713 1093 1277 1820 1878 1914 2004 2418 2666
Size: 380 184 543 58 36 90 414 248
Cuts at: 2666 2701 713
Size: 35 3000
Fragments arranged by size:
3000 543 414 380 248 184 90 58
36 35
BanII G_rGCy'C
Cuts at: 380 1742 2002 3032 380
Size: 1362 260 1030 2336
Fragments arranged by size:
2336 1362 1030 260
BbeI G_GCGC'C
Cuts at: 2422 2670 2422
Size: 248 4740
BbvCI CC'TCA_GC
Cuts at: 466 466
Size: 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1003 1037 1003
Size: 34 4954
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2886 3647 4988 1623
Size: 1263 761 1341 1623
Fragments arranged by size:
1623 1341 1263 761
BclI T'GATC_A
Cuts at: 456 2508 456
Size: 2052 2936
BglI GCCn_nnn'nGGC
Cuts at: 521 521
Size: 4988
BglII A'GATC_T
Cuts at: 2503 4540 2503
Size: 2037 2951
BlpI GC'TnA_GC
Cuts at: 2100 2100
Size: 4988
Bme1580I G_kGCm'C
Cuts at: 380 545 716 1883 2613 2706 3762 380
Size: 165 171 1167 730 93 1056 1606
Fragments arranged by size:
1606 1167 1056 730 171 165 93
BmrI ACTGGGnnnn_n'
Cuts at: 2200 2281 2616 4760 2200
Size: 81 335 2144 2428
Fragments arranged by size:
2428 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 4988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 694 867 1184 1732 662
Size: 32 173 317 548 3918
Fragments arranged by size:
3918 548 317 173 32
Bpu10I CC'TnA_GC
Cuts at: 466 466
Size: 4988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 2164 3043 3535 3833 1009
Size: 1155 879 492 298 2164
Fragments arranged by size:
2164 1155 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 494 4692 494
Size: 4198 790
BsaAI yAC'GTr
Cuts at: 1386 1963 1980 2341 1386
Size: 577 17 361 4033
Fragments arranged by size:
4033 577 361 17
BsaBI GATnn'nnATC
Cuts at: 6 2502 4539 6
Size: 2496 2037 455
Fragments arranged by size:
2496 2037 455
BsaHI Gr'CG_yC
Cuts at: 397 1340 2419 2667 3369 4865 397
Size: 943 1079 248 702 1496 520
Fragments arranged by size:
1496 1079 943 702 520 248
BsaWI w'CCGG_w
Cuts at: 283 629 1973 2177 2698 3376 3650 3797 4208
Size: 346 1344 204 521 678 274 147 411
Cuts at: 4208 283
Size: 1063
Fragments arranged by size:
1344 1063 678 521 411 346 274 204
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1932 1962 1932
Size: 30 4958
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 291 1750 291
Size: 1459 3529
BseYI C'CCAG_C
Cuts at: 373 1436 1831 1896 2413 3171 3748 4244 373
Size: 1063 395 65 517 758 577 496 1117
Fragments arranged by size:
1117 1063 758 577 517 496 395 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1812 1840 1812
Size: 28 4960
BsiEI CG_ry'CG
Cuts at: 104 2530 2576 3784 104
Size: 2426 46 1208 1308
Fragments arranged by size:
2426 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 545 902 1742 2002 2780 2970 3762 545
Size: 357 840 260 778 190 792 1771
Fragments arranged by size:
1771 840 792 778 357 260 190
BsmBI CGTCTCn'nnnn_
Cuts at: 388 1077 388
Size: 689 4299
Bsp1286I G_dGCh'C
Cuts at: 380 545 716 902 1742 1883 2002 2613 2706
Size: 165 171 186 840 141 119 611 93
Cuts at: 2706 2780 2970 3032 3762 380
Size: 74 190 62 730 1606
Fragments arranged by size:
1606 840 730 611 190 186 171 165
141 119 93 74 62
BspEI T'CCGG_A
Cuts at: 629 4208 629
Size: 3579 1409
BspHI T'CATG_A
Cuts at: 4164 4164
Size: 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1902 2038 2252 2554 2935 1902
Size: 136 214 302 381 3955
Fragments arranged by size:
3955 381 302 214 136
BsrBI CCG'CTC
Cuts at: 50 3280 3334 4818 4983 50
Size: 3230 54 1484 165 55
Fragments arranged by size:
3230 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 2900 1931
Size: 969 4019
BsrFI r'CCGG_y
Cuts at: 1190 1872 2986 3167 3376 1190
Size: 682 1114 181 209 2802
Fragments arranged by size:
2802 1114 682 209 181
BssSI C'ACGA_G
Cuts at: 3259 3617 3259
Size: 358 4630
BstAPI GCAn_nnn'nTGC
Cuts at: 1386 2365 1386
Size: 979 4009
BstBI TT'CG_AA
Cuts at: 110 3349 110
Size: 3239 1749
BstEII G'GTnAC_C
Cuts at: 1665 1665
Size: 4988
BstXI CCAn_nnnn'nTGG
Cuts at: 250 441 871 1963 250
Size: 191 430 1092 3275
Fragments arranged by size:
3275 1092 430 191
BstYI r'GATC_y
Cuts at: 1 993 1216 1500 2013 2480 2503 2838 3084
Size: 992 223 284 513 467 23 335 246
Cuts at: 3084 4096 4182 4194 4540 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 992 513 467 449 346 335 284
246 223 86 23 12
BtgI C'CryG_G
Cuts at: 125 519 721 1826 125
Size: 394 202 1105 3287
Fragments arranged by size:
3287 1105 394 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2938 3119 3282 2938
Size: 181 163 4644
Fragments arranged by size:
4644 181 163
BtsI GCAGTG_nn'
Cuts at: 543 1151 1241 1380 1774 1800 1867 2231 543
Size: 608 90 139 394 26 67 364 3300
Fragments arranged by size:
3300 608 394 364 139 90 67 26
DraI TTT'AAA
Cuts at: 1987 1987
Size: 4988
DraIII CAC_nnn'GTG
Cuts at: 1827 1827
Size: 4988
DrdI GACnn_nn'nnGTC
Cuts at: 1261 2694 3552 1261
Size: 1433 858 2697
Fragments arranged by size:
2697 1433 858
EaeI y'GGCC_r
Cuts at: 128 724 1589 2573 2747 3138 3165 4510 128
Size: 596 865 984 174 391 27 1345 606
Fragments arranged by size:
1345 984 865 606 596 391 174 27
EagI C'GGCC_G
Cuts at: 2573 2573
Size: 4988
EarI CTCTTCn'nnn_
Cuts at: 1136 1750 3011 3221 1136
Size: 614 1261 210 2903
Fragments arranged by size:
2903 1261 614 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3196 3506 3652 4798 3196
Size: 310 146 1146 3386
Fragments arranged by size:
3386 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1740 2000 1740
Size: 260 4728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 694 867 950 1025 1184 1732 2813 3245
Size: 32 173 83 75 159 548 1081 432
Cuts at: 3245 3992 4260 662
Size: 747 268 1390
Fragments arranged by size:
1390 1081 747 548 432 268 173 159
83 75 32
EcoNI CCTnn'n_nnAGG
Cuts at: 1042 1042
Size: 4988
EcoO109I rG'GnC_Cy
Cuts at: 377 2127 377
Size: 1750 3238
EcoRI G'AATT_C
Cuts at: 1992 1992
Size: 4988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4988
FspI TGC'GCA
Cuts at: 2769 2769
Size: 4988
HaeII r_GCGC'y
Cuts at: 431 761 2422 2670 3692 4671 431
Size: 330 1661 248 1022 979 748
Fragments arranged by size:
1661 1022 979 748 330 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1057 1089 1621 1653 1932 1964 2830 2860 2862
Size: 32 532 32 279 32 866 30 2
Cuts at: 2862 2892 1057
Size: 30 3153
Fragments arranged by size:
3153 866 532 279 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2027 2027
Size: 4988
KasI G'GCGC_C
Cuts at: 2418 2666 2418
Size: 248 4740
KpnI G_GTAC'C
Cuts at: 1097 1281 2008 1097
Size: 184 727 4077
Fragments arranged by size:
4077 727 184
MluI A'CGCG_T
Cuts at: 4421 4421
Size: 4988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 329 3659 3843 329
Size: 3330 184 1474
Fragments arranged by size:
3330 1474 184
MscI TGG'CCA
Cuts at: 1591 2749 1591
Size: 1158 3830
MslI CAynn'nnrTG
Cuts at: 510 2977 3104 510
Size: 2467 127 2394
Fragments arranged by size:
2467 2394 127
MspA1I CmG'CkG
Cuts at: 686 1440 1788 1900 2099 2413 2773 3786 4031
Size: 754 348 112 199 314 360 1013 245
Cuts at: 4031 686
Size: 1643
Fragments arranged by size:
1643 1013 754 360 348 314 245 199
112
NaeI GCC'GGC
Cuts at: 1192 3169 1192
Size: 1977 3011
NarI GG'CG_CC
Cuts at: 2419 2667 2419
Size: 248 4740
NcoI C'CATG_G
Cuts at: 125 125
Size: 4988
NgoMIV G'CCGG_C
Cuts at: 1190 3167 1190
Size: 1977 3011
NspI r_CATG'y
Cuts at: 612 793 1188 2041 2541 3448 612
Size: 181 395 853 500 907 2152
Fragments arranged by size:
2152 907 853 500 395 181
PciI A'CATG_T
Cuts at: 608 1184 3444 608
Size: 576 2260 2152
Fragments arranged by size:
2260 2152 576
PflMI CCAn_nnn'nTGG
Cuts at: 1587 1587
Size: 4988
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4988
PmlI CAC'GTG
Cuts at: 1963 1963
Size: 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4956
PsiI TTA'TAA
Cuts at: 1403 1403
Size: 4988
PspOMI G'GGCC_C
Cuts at: 376 376
Size: 4988
PspXI vC'TCGA_Gb
Cuts at: 363 363
Size: 4988
PstI C_TGCA'G
Cuts at: 1158 1236 1691 2035 2720 1158
Size: 78 455 344 685 3426
Fragments arranged by size:
3426 685 455 344 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4988
PvuII CAG'CTG
Cuts at: 686 1440 1788 1900 2413 2773 686
Size: 754 348 112 513 360 2901
Fragments arranged by size:
2901 754 513 360 348 112
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4988
SacI G_AGCT'C
Cuts at: 1742 2002 1742
Size: 260 4728
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4988
SapI GCTCTTCn'nnn_
Cuts at: 3011 3221 3011
Size: 210 4778
SbfI CC_TGCA'GG
Cuts at: 2035 2035
Size: 4988
ScaI AGT'ACT
Cuts at: 4505 4505
Size: 4988
SexAI A'CCwGG_T
Cuts at: 1280 1280
Size: 4988
SfcI C'TryA_G
Cuts at: 33 1104 1154 1232 1241 1687 2031 2716 3709
Size: 1071 50 78 9 446 344 685 993
Cuts at: 3709 3900 33
Size: 191 1121
Fragments arranged by size:
1121 1071 993 685 446 344 191 78
50 9
SfoI GGC'GCC
Cuts at: 2420 2668 2420
Size: 248 4740
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
SmaI CCC'GGG
Cuts at: 2010 2010
Size: 4988
SmlI C'TyrA_G
Cuts at: 363 589 988 2143 3058 3550 3812 363
Size: 226 399 1155 915 492 262 1539
Fragments arranged by size:
1539 1155 915 492 399 262 226
SnaBI TAC'GTA
Cuts at: 1980 1980
Size: 4988
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4988
SphI G_CATG'C
Cuts at: 2041 2541 2041
Size: 500 4488
StyI C'CwwG_G
Cuts at: 125 915 1361 1496 2122 2476 125
Size: 790 446 135 626 354 2637
Fragments arranged by size:
2637 790 626 446 354 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1450 1552 1910 2571 2704 3163 3372 1450
Size: 102 358 661 133 459 209 3066
Fragments arranged by size:
3066 661 459 358 209 133 102
TatI w'GTAC_w
Cuts at: 739 782 4503 739
Size: 43 3721 1224
Fragments arranged by size:
3721 1224 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1562 1594 4505 4537 1562
Size: 32 2911 32 2013
Fragments arranged by size:
2911 2013 32 32
Tth111I GACn'n_nGTC
Cuts at: 1069 2785 1069
Size: 1716 3272
XbaI T'CTAG_A
Cuts at: 555 2019 555
Size: 1464 3524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 160 1296 1904 2483 160
Size: 1136 608 579 2665
Fragments arranged by size:
2665 1136 608 579
XhoI C'TCGA_G
Cuts at: 363 589 363
Size: 226 4762
XmaI C'CCGG_G
Cuts at: 2008 2008
Size: 4988
ZraI GAC'GTC
Cuts at: 398 398
Size: 4988
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PsrI SacII SanDI SfiI
SgrAI SrfI SspI StuI SwaI XmnI