Restriction map for
pF1KSDA1479
(Circular) (Six-base) MAPSORT of: pF1KSDA1479.seq Check: 647 from: 1 to: 6170
pF1KSDA1479 6170 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1966 1966
Size: 6170
AccI GT'mk_AC
Cuts at: 224 1988 2266 3208 224
Size: 1764 278 942 3186
Fragments arranged by size:
3186 1764 942 278
Acc65I G'GTAC_C
Cuts at: 535 3186 535
Size: 2651 3519
AclI AA'CG_TT
Cuts at: 6010 6010
Size: 6170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 254 1475 1835 2608 3995 4427 5174 5442
Size: 36 1221 360 773 1387 432 747 268
Cuts at: 5442 218
Size: 946
Fragments arranged by size:
1387 1221 946 773 747 432 360 268
36
AflIII A'CryG_T
Cuts at: 2003 2453 3522 4626 5603 2003
Size: 450 1069 1104 977 2570
Fragments arranged by size:
2570 1104 1069 977 450
AgeI A'CCGG_T
Cuts at: 4558 4558
Size: 6170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2007 2007
Size: 6170
AlwNI CAG_nnn'CTG
Cuts at: 691 1180 1667 3475 5042 691
Size: 489 487 1808 1567 1819
Fragments arranged by size:
1819 1808 1567 489 487
ApaLI G'TGCA_C
Cuts at: 4940 4940
Size: 6170
ApoI r'AATT_y
Cuts at: 317 1837 2838 3174 5523 5779 317
Size: 1520 1001 336 2349 256 708
Fragments arranged by size:
2349 1520 1001 708 336 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6170
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6170
AvaI C'yCGr_G
Cuts at: 1370 3190 1370
Size: 1820 4350
AvrII C'CTAG_G
Cuts at: 3052 3052
Size: 6170
BamHI G'GATC_C
Cuts at: 1741 2667 3195 1741
Size: 926 528 4716
Fragments arranged by size:
4716 926 528
BanI G'GyrC_C
Cuts at: 535 3186 3600 3848 3883 535
Size: 2651 414 248 35 2822
Fragments arranged by size:
2822 2651 414 248 35
BanII G_rGCy'C
Cuts at: 1355 3184 4214 1355
Size: 1829 1030 3311
Fragments arranged by size:
3311 1829 1030
BbeI G_GCGC'C
Cuts at: 3604 3852 3604
Size: 248 5922
BbsI GAAGACnn'nnnn_
Cuts at: 126 1208 1580 1790 2293 126
Size: 1082 372 210 503 4003
Fragments arranged by size:
4003 1082 503 372 210
BciVI GTATCCnnnnn_n'
Cuts at: 4068 4829 6170 4068
Size: 761 1341 4068
Fragments arranged by size:
4068 1341 761
BclI T'GATC_A
Cuts at: 2508 3690 2508
Size: 1182 4988
BfrBI ATG'CAT
Cuts at: 544 544
Size: 6170
BglI GCCn_nnn'nGGC
Cuts at: 272 688 272
Size: 416 5754
BglII A'GATC_T
Cuts at: 3685 5722 3685
Size: 2037 4133
BlpI GC'TnA_GC
Cuts at: 3282 3282
Size: 6170
Bme1580I G_kGCm'C
Cuts at: 3795 3888 4944 3795
Size: 93 1056 5021
Fragments arranged by size:
5021 1056 93
BmgBI CAC'GTC
Cuts at: 2456 3093 2456
Size: 637 5533
BmrI ACTGGGnnnn_n'
Cuts at: 168 2046 2408 2963 3382 3463 3798 5942 168
Size: 1878 362 555 419 81 335 2144 396
Fragments arranged by size:
2144 1878 555 419 396 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4537 4537
Size: 6170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1007 1157 1916 2052 2219 2813 3000 955
Size: 52 150 759 136 167 594 187 4125
Fragments arranged by size:
4125 759 594 187 167 150 136 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3346 4225 4717 5015 3346
Size: 879 492 298 4501
Fragments arranged by size:
4501 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2101 5874 2101
Size: 3773 2397
BsaAI yAC'GTr
Cuts at: 3162 3523 3162
Size: 361 5809
BsaBI GATnn'nnATC
Cuts at: 6 1607 3684 5721 6
Size: 1601 2077 2037 455
Fragments arranged by size:
2077 2037 1601 455
BsaHI Gr'CG_yC
Cuts at: 264 3073 3601 3849 4551 6047 264
Size: 2809 528 248 702 1496 387
Fragments arranged by size:
2809 1496 702 528 387 248
BsaWI w'CCGG_w
Cuts at: 249 3359 3880 4558 4832 4979 5390 249
Size: 3110 521 678 274 147 411 1029
Fragments arranged by size:
3110 1029 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 923 3595 4353 4930 5426 923
Size: 2672 758 577 496 1667
Fragments arranged by size:
2672 1667 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1575 1575
Size: 6170
BsiEI CG_ry'CG
Cuts at: 104 3712 3758 4966 104
Size: 3608 46 1208 1308
Fragments arranged by size:
3608 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3184 3962 4152 4944 3184
Size: 778 190 792 4410
Fragments arranged by size:
4410 792 778 190
BsmI GAATG_Cn'
Cuts at: 481 544 1966 481
Size: 63 1422 4685
Fragments arranged by size:
4685 1422 63
Bsp1286I G_dGCh'C
Cuts at: 1355 3184 3795 3888 3962 4152 4214 4944 1355
Size: 1829 611 93 74 190 62 730 2581
Fragments arranged by size:
2581 1829 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5390 5390
Size: 6170
BspHI T'CATG_A
Cuts at: 5346 5346
Size: 6170
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1966 2168 3220 3434 3736 4117 1966
Size: 202 1052 214 302 381 4019
Fragments arranged by size:
4019 1052 381 302 214 202
BsrBI CCG'CTC
Cuts at: 50 4462 4516 6000 6165 50
Size: 4412 54 1484 165 55
Fragments arranged by size:
4412 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 282 1340 1970 4082 282
Size: 1058 630 2112 2370
Fragments arranged by size:
2370 2112 1058 630
BsrFI r'CCGG_y
Cuts at: 2546 4168 4349 4558 2546
Size: 1622 181 209 4158
Fragments arranged by size:
4158 1622 209 181
BssSI C'ACGA_G
Cuts at: 1709 4441 4799 1709
Size: 2732 358 3080
Fragments arranged by size:
3080 2732 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1972 3547 1972
Size: 1575 4595
BstBI TT'CG_AA
Cuts at: 110 4531 110
Size: 4421 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1401 1911 2214 2332 2772 1401
Size: 510 303 118 440 4799
Fragments arranged by size:
4799 510 440 303 118
BstYI r'GATC_y
Cuts at: 1 739 1741 2027 2667 3195 3662 3685 4020
Size: 738 1002 286 640 528 467 23 335
Cuts at: 4020 4266 5278 5364 5376 5722 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 1002 738 640 528 467 449 346
335 286 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1989 1989
Size: 6170
BtgI C'CryG_G
Cuts at: 1869 2207 2325 2331 2672 2928 1869
Size: 338 118 6 341 256 5111
Fragments arranged by size:
5111 341 338 256 118 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 722 2705 4120 4301 4464 722
Size: 1983 1415 181 163 2428
Fragments arranged by size:
2428 1983 1415 181 163
BtsI GCAGTG_nn'
Cuts at: 1563 3413 1563
Size: 1850 4320
ClaI AT'CG_AT
Cuts at: 1274 2680 1274
Size: 1406 4764
DraI TTT'AAA
Cuts at: 3169 3169
Size: 6170
DrdI GACnn_nn'nnGTC
Cuts at: 1582 2110 3876 4734 1582
Size: 528 1766 858 3018
Fragments arranged by size:
3018 1766 858 528
EaeI y'GGCC_r
Cuts at: 701 1391 2328 3755 3929 4320 4347 5692 701
Size: 690 937 1427 174 391 27 1345 1179
Fragments arranged by size:
1427 1345 1179 937 690 391 174 27
EagI C'GGCC_G
Cuts at: 3755 3755
Size: 6170
EarI CTCTTCn'nnn_
Cuts at: 1520 2255 2299 3053 4193 4403 1520
Size: 735 44 754 1140 210 3287
Fragments arranged by size:
3287 1140 754 735 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2037 2366 4378 4688 4834 5980 2037
Size: 329 2012 310 146 1146 2227
Fragments arranged by size:
2227 2012 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 3182 3182
Size: 6170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 254 955 1007 1157 1475 1835 1916 2052
Size: 36 701 52 150 318 360 81 136
Cuts at: 2052 2219 2608 2813 3000 3995 4427 5174 5442
Size: 167 389 205 187 995 432 747 268
Cuts at: 5442 218
Size: 946
Fragments arranged by size:
995 946 747 701 432 389 360 318
268 205 187 167 150 136 81 52
36
EcoNI CCTnn'n_nnAGG
Cuts at: 2847 2847
Size: 6170
EcoO109I rG'GnC_Cy
Cuts at: 930 2691 3309 930
Size: 1761 618 3791
Fragments arranged by size:
3791 1761 618
EcoRI G'AATT_C
Cuts at: 1837 3174 1837
Size: 1337 4833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3126 4189 3126
Size: 1063 5107
FspI TGC'GCA
Cuts at: 3951 3951
Size: 6170
HaeII r_GCGC'y
Cuts at: 3604 3852 4874 5853 3604
Size: 248 1022 979 3921
Fragments arranged by size:
3921 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1871 1903 2029 2061 2188 2220 2762 2794 2982
Size: 32 126 32 127 32 542 32 188
Cuts at: 2982 3014 4012 4042 4044 4074 1871
Size: 32 998 30 2 30 3967
Fragments arranged by size:
3967 998 542 188 127 126 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 225 1778 3209 225
Size: 1553 1431 3186
Fragments arranged by size:
3186 1553 1431
HindIII A'AGCT_T
Cuts at: 1256 2494 1256
Size: 1238 4932
HpaI GTT'AAC
Cuts at: 1778 1778
Size: 6170
KasI G'GCGC_C
Cuts at: 3600 3848 3600
Size: 248 5922
KpnI G_GTAC'C
Cuts at: 539 3190 539
Size: 2651 3519
MluI A'CGCG_T
Cuts at: 5603 5603
Size: 6170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1580 1928 2152 4841 5025 1580
Size: 348 224 2689 184 2725
Fragments arranged by size:
2725 2689 348 224 184
MscI TGG'CCA
Cuts at: 703 2330 3931 703
Size: 1627 1601 2942
Fragments arranged by size:
2942 1627 1601
MslI CAynn'nnrTG
Cuts at: 470 1399 1922 2036 2212 2330 2597 2641 4159
Size: 929 523 114 176 118 267 44 1518
Cuts at: 4159 4286 470
Size: 127 2354
Fragments arranged by size:
2354 1518 929 523 267 176 127 118
114 44
MspA1I CmG'CkG
Cuts at: 306 927 3281 3595 3955 4968 5213 306
Size: 621 2354 314 360 1013 245 1263
Fragments arranged by size:
2354 1263 1013 621 360 314 245
NaeI GCC'GGC
Cuts at: 4351 4351
Size: 6170
NarI GG'CG_CC
Cuts at: 3601 3849 3601
Size: 248 5922
NcoI C'CATG_G
Cuts at: 1869 2207 2325 2331 2672 1869
Size: 338 118 6 341 5367
Fragments arranged by size:
5367 341 338 118 6
NdeI CA'TA_TG
Cuts at: 898 898
Size: 6170
NgoMIV G'CCGG_C
Cuts at: 4349 4349
Size: 6170
NsiI A_TGCA'T
Cuts at: 546 546
Size: 6170
NspI r_CATG'y
Cuts at: 2007 3223 3723 4630 2007
Size: 1216 500 907 3547
Fragments arranged by size:
3547 1216 907 500
PasI CC'CwG_GG
Cuts at: 1762 1762
Size: 6170
PciI A'CATG_T
Cuts at: 2003 4626 2003
Size: 2623 3547
PflMI CCAn_nnn'nTGG
Cuts at: 1225 2191 1225
Size: 966 5204
PfoI T'CCnGG_A
Cuts at: 932 1280 2790 932
Size: 348 1510 4312
Fragments arranged by size:
4312 1510 348
PmeI GTTT'AAAC
Cuts at: 3169 3169
Size: 6170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1342 1374 5309 5341 1342
Size: 32 3935 32 2171
Fragments arranged by size:
3935 2171 32 32
PpuMI rG'GwC_Cy
Cuts at: 930 2691 930
Size: 1761 4409
PshAI GACnn'nnGTC
Cuts at: 1372 1372
Size: 6170
PsiI TTA'TAA
Cuts at: 1262 1262
Size: 6170
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1460 1492 1460
Size: 32 6138
PstI C_TGCA'G
Cuts at: 1019 2297 3005 3217 3902 1019
Size: 1278 708 212 685 3287
Fragments arranged by size:
3287 1278 708 685 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6170
PvuII CAG'CTG
Cuts at: 306 3595 3955 306
Size: 3289 360 2521
Fragments arranged by size:
3289 2521 360
RsrII CG'GwC_CG
Cuts at: 4365 4365
Size: 6170
SacI G_AGCT'C
Cuts at: 3184 3184
Size: 6170
SalI G'TCGA_C
Cuts at: 223 3207 223
Size: 2984 3186
SapI GCTCTTCn'nnn_
Cuts at: 2255 2299 4193 4403 2255
Size: 44 1894 210 4022
Fragments arranged by size:
4022 1894 210 44
SbfI CC_TGCA'GG
Cuts at: 2297 3217 2297
Size: 920 5250
ScaI AGT'ACT
Cuts at: 5687 5687
Size: 6170
SfcI C'TryA_G
Cuts at: 33 567 1015 2293 3001 3213 3898 4891 5082
Size: 534 448 1278 708 212 685 993 191
Cuts at: 5082 33
Size: 1121
Fragments arranged by size:
1278 1121 993 708 685 534 448 212
191
SfoI GGC'GCC
Cuts at: 3602 3850 3602
Size: 248 5922
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6170
SgrAI Cr'CCGG_yG
Cuts at: 2546 2546
Size: 6170
SmaI CCC'GGG
Cuts at: 3192 3192
Size: 6170
SmlI C'TyrA_G
Cuts at: 3325 4240 4732 4994 3325
Size: 915 492 262 4501
Fragments arranged by size:
4501 915 492 262
SnaBI TAC'GTA
Cuts at: 3162 3162
Size: 6170
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6170
SphI G_CATG'C
Cuts at: 3223 3723 3223
Size: 500 5670
SspI AAT'ATT
Cuts at: 2133 2133
Size: 6170
StuI AGG'CCT
Cuts at: 1224 1224
Size: 6170
StyI C'CwwG_G
Cuts at: 1219 1869 2207 2325 2331 2672 3052 3304 3658
Size: 650 338 118 6 341 380 252 354
Cuts at: 3658 1219
Size: 3731
Fragments arranged by size:
3731 650 380 354 341 338 252 118
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 721 904 1353 2202 3753 3886 4345 4554 721
Size: 183 449 849 1551 133 459 209 2337
Fragments arranged by size:
2337 1551 849 459 449 209 183 133
TatI w'GTAC_w
Cuts at: 5685 5685
Size: 6170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2687 2719 2824 2856 2972 3004 5687 5719 2687
Size: 32 105 32 116 32 2683 32 3138
Fragments arranged by size:
3138 2683 116 105 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3075 3967 3075
Size: 892 5278
XbaI T'CTAG_A
Cuts at: 3201 3201
Size: 6170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1181 1744 2625 3665 1181
Size: 563 881 1040 3686
Fragments arranged by size:
3686 1040 881 563
XmaI C'CCGG_G
Cuts at: 3190 3190
Size: 6170
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
BaeI BbvCI BcgI BmtI Bpu10I BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstEII Bsu36I CspCI DraIII EcoRV
FseI FspAI MfeI NheI NotI NruI PacI PmlI
PspOMI PspXI SacII SanDI SexAI SfiI SrfI SwaI
XhoI XmnI ZraI