Restriction map for
pF1KSDA1465
(Circular) (Six-base) MAPSORT of: pF1KSDA1465.seq Check: 4273 from: 1 to: 5372
pF1KSDA1465 5372 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 347 347
Size: 5372
AccI GT'mk_AC
Cuts at: 1181 2410 1181
Size: 1229 4143
Acc65I G'GTAC_C
Cuts at: 1807 2388 1807
Size: 581 4791
AclI AA'CG_TT
Cuts at: 5212 5212
Size: 5372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 3197 3629 4376 4644 931
Size: 2266 432 747 268 1659
Fragments arranged by size:
2266 1659 747 432 268
AfeI AGC'GCT
Cuts at: 1498 1498
Size: 5372
AflIII A'CryG_T
Cuts at: 1230 1538 2724 3828 4805 1230
Size: 308 1186 1104 977 1797
Fragments arranged by size:
1797 1186 1104 977 308
AgeI A'CCGG_T
Cuts at: 981 3760 981
Size: 2779 2593
AhdI GACnn_n'nnGTC
Cuts at: 293 293
Size: 5372
AleI CACnn'nnGTG
Cuts at: 724 724
Size: 5372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 854 889 854
Size: 35 5337
AlwNI CAG_nnn'CTG
Cuts at: 2677 4244 2677
Size: 1567 3805
ApaI G_GGCC'C
Cuts at: 144 1256 1657 1664 144
Size: 1112 401 7 3852
Fragments arranged by size:
3852 1112 401 7
ApaLI G'TGCA_C
Cuts at: 815 1193 4142 815
Size: 378 2949 2045
Fragments arranged by size:
2949 2045 378
ApoI r'AATT_y
Cuts at: 2376 4725 4981 2376
Size: 2349 256 2767
Fragments arranged by size:
2767 2349 256
AseI AT'TA_AT
Cuts at: 20 2333 20
Size: 2313 3059
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
AvaI C'yCGr_G
Cuts at: 542 722 736 1363 1408 1690 1986 2080 2392
Size: 180 14 627 45 282 296 94 312
Cuts at: 2392 542
Size: 3522
Fragments arranged by size:
3522 627 312 296 282 180 94 45
14
BamHI G'GATC_C
Cuts at: 2397 2397
Size: 5372
BanI G'GyrC_C
Cuts at: 258 612 619 783 865 961 1098 1149 1210
Size: 354 7 164 82 96 137 51 61
Cuts at: 1210 1273 1807 1954 2302 2388 2802 3050 3085
Size: 63 534 147 348 86 414 248 35
Cuts at: 3085 258
Size: 2545
Fragments arranged by size:
2545 534 414 354 348 248 164 147
137 96 86 82 63 61 51 35
7
BanII G_rGCy'C
Cuts at: 144 535 743 1256 1562 1657 1664 1683 1886
Size: 391 208 513 306 95 7 19 203
Cuts at: 1886 2275 2386 3416 144
Size: 389 111 1030 2100
Fragments arranged by size:
2100 1030 513 391 389 306 208 203
111 95 19 7
BbeI G_GCGC'C
Cuts at: 616 1102 1214 1277 1958 2306 2806 3054 616
Size: 486 112 63 681 348 500 248 2934
Fragments arranged by size:
2934 681 500 486 348 248 112 63
BbsI GAAGACnn'nnnn_
Cuts at: 1469 1566 2168 1469
Size: 97 602 4673
Fragments arranged by size:
4673 602 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 733 767 2050 2084 733
Size: 34 1283 34 4021
Fragments arranged by size:
4021 1283 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3270 4031 5372 3270
Size: 761 1341 3270
Fragments arranged by size:
3270 1341 761
BclI T'GATC_A
Cuts at: 2892 2892
Size: 5372
BglI GCCn_nnn'nGGC
Cuts at: 1171 1171
Size: 5372
BglII A'GATC_T
Cuts at: 2887 4924 2887
Size: 2037 3335
BlpI GC'TnA_GC
Cuts at: 634 2484 634
Size: 1850 3522
Bme1580I G_kGCm'C
Cuts at: 144 819 1154 1197 1256 1657 1664 1946 2997
Size: 675 335 43 59 401 7 282 1051
Cuts at: 2997 3090 4146 144
Size: 93 1056 1370
Fragments arranged by size:
1370 1056 1051 675 401 335 282 93
59 43 7
BmgBI CAC'GTC
Cuts at: 359 1572 2139 359
Size: 1213 567 3592
Fragments arranged by size:
3592 1213 567
BmrI ACTGGGnnnn_n'
Cuts at: 2232 2584 2665 3000 5144 2232
Size: 352 81 335 2144 2460
Fragments arranged by size:
2460 2144 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 762 2289 3739 762
Size: 1527 1450 2395
Fragments arranged by size:
2395 1527 1450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1064 1064
Size: 5372
Bpu10I CC'TnA_GC
Cuts at: 490 490
Size: 5372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1546 2548 3427 3919 4217 412
Size: 1134 1002 879 492 298 1567
Fragments arranged by size:
1567 1134 1002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1408 2171 5076 1408
Size: 763 2905 1704
Fragments arranged by size:
2905 1704 763
BsaAI yAC'GTr
Cuts at: 1854 2364 2725 1854
Size: 510 361 4501
Fragments arranged by size:
4501 510 361
BsaBI GATnn'nnATC
Cuts at: 6 2886 4923 6
Size: 2880 2037 455
Fragments arranged by size:
2880 2037 455
BsaHI Gr'CG_yC
Cuts at: 344 613 1099 1178 1211 1274 1586 1772 1955
Size: 269 486 79 33 63 312 186 183
Cuts at: 1955 2303 2803 3051 3753 5249 344
Size: 348 500 248 702 1496 467
Fragments arranged by size:
1496 702 500 486 467 348 312 269
248 186 183 79 63 33
BsaWI w'CCGG_w
Cuts at: 981 2561 3082 3760 4034 4181 4592 981
Size: 1580 521 678 274 147 411 1761
Fragments arranged by size:
1761 1580 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1062 1092 2224 2254 1062
Size: 30 1132 30 4180
Fragments arranged by size:
4180 1132 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1447 1468 1504 2140 2218 2272 1447
Size: 21 36 636 78 54 4547
Fragments arranged by size:
4547 636 78 54 36 21
BseYI C'CCAG_C
Cuts at: 823 1104 1206 1581 1649 2797 3555 4132 4628
Size: 281 102 375 68 1148 758 577 496
Cuts at: 4628 823
Size: 1567
Fragments arranged by size:
1567 1148 758 577 496 375 281 102
68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 1768 2161 1645
Size: 123 393 4856
Fragments arranged by size:
4856 393 123
BsiEI CG_ry'CG
Cuts at: 104 1804 2289 2914 2960 4168 104
Size: 1700 485 625 46 1208 1308
Fragments arranged by size:
1700 1308 1208 625 485 46
BsiHKAI G_wGCw'C
Cuts at: 721 819 1197 1562 1886 2386 3164 3354 4146
Size: 98 378 365 324 500 778 190 792
Cuts at: 4146 721
Size: 1947
Fragments arranged by size:
1947 792 778 500 378 365 324 190
98
BsiWI C'GTAC_G
Cuts at: 604 604
Size: 5372
BsmI GAATG_Cn'
Cuts at: 1403 1403
Size: 5372
BsmBI CGTCTCn'nnnn_
Cuts at: 1414 1414
Size: 5372
Bsp1286I G_dGCh'C
Cuts at: 144 535 721 743 819 1154 1197 1256 1562
Size: 391 186 22 76 335 43 59 306
Cuts at: 1562 1657 1664 1683 1886 1946 2275 2386 2997
Size: 95 7 19 203 60 329 111 611
Cuts at: 2997 3090 3164 3354 3416 4146 144
Size: 93 74 190 62 730 1370
Fragments arranged by size:
1370 730 611 391 335 329 306 203
190 186 111 95 93 76 74 62
60 59 43 22 19 7
BspEI T'CCGG_A
Cuts at: 4592 4592
Size: 5372
BspHI T'CATG_A
Cuts at: 4548 4548
Size: 5372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 488 578 2201 2422 2636 2938 3319 488
Size: 90 1623 221 214 302 381 2541
Fragments arranged by size:
2541 1623 381 302 221 214 90
BsrBI CCG'CTC
Cuts at: 50 992 3664 3718 5202 5367 50
Size: 942 2672 54 1484 165 55
Fragments arranged by size:
2672 1484 942 165 55 54
BsrDI GCAATG_nn'
Cuts at: 749 943 3284 749
Size: 194 2341 2837
Fragments arranged by size:
2837 2341 194
BsrFI r'CCGG_y
Cuts at: 596 786 981 1095 3370 3551 3760 596
Size: 190 195 114 2275 181 209 2208
Fragments arranged by size:
2275 2208 209 195 190 181 114
BssHII G'CGCG_C
Cuts at: 494 638 876 1645 494
Size: 144 238 769 4221
Fragments arranged by size:
4221 769 238 144
BssSI C'ACGA_G
Cuts at: 3643 4001 3643
Size: 358 5014
BstAPI GCAn_nnn'nTGC
Cuts at: 556 783 2749 556
Size: 227 1966 3179
Fragments arranged by size:
3179 1966 227
BstBI TT'CG_AA
Cuts at: 110 3733 110
Size: 3623 1749
BstYI r'GATC_y
Cuts at: 1 950 1469 2397 2864 2887 3222 3468 4480
Size: 949 519 928 467 23 335 246 1012
Cuts at: 4480 4566 4578 4924 1
Size: 86 12 346 449
Fragments arranged by size:
1012 949 928 519 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2018 2018
Size: 5372
BtgI C'CryG_G
Cuts at: 392 469 1791 392
Size: 77 1322 3973
Fragments arranged by size:
3973 1322 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 891 1575 1874 2031 2304 3322 3503 3666 891
Size: 684 299 157 273 1018 181 163 2597
Fragments arranged by size:
2597 1018 684 299 273 181 163 157
BtsI GCAGTG_nn'
Cuts at: 675 871 900 1751 1756 1916 2615 675
Size: 196 29 851 5 160 699 3432
Fragments arranged by size:
3432 851 699 196 160 29 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1214 1249 1214
Size: 35 5337
DraI TTT'AAA
Cuts at: 2371 2371
Size: 5372
DraIII CAC_nnn'GTG
Cuts at: 556 556
Size: 5372
DrdI GACnn_nn'nnGTC
Cuts at: 3078 3936 3078
Size: 858 4514
EaeI y'GGCC_r
Cuts at: 173 410 915 1093 1391 1801 1934 2957 3131
Size: 237 505 178 298 410 133 1023 174
Cuts at: 3131 3522 3549 4894 173
Size: 391 27 1345 651
Fragments arranged by size:
1345 1023 651 505 410 391 298 237
178 174 133 27
EagI C'GGCC_G
Cuts at: 1801 2957 1801
Size: 1156 4216
EarI CTCTTCn'nnn_
Cuts at: 3395 3605 3395
Size: 210 5162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1030 1501 1621 1691 3580 3890 4036 5182 1030
Size: 471 120 70 1889 310 146 1146 1220
Fragments arranged by size:
1889 1220 1146 471 310 146 120 70
EcoICRI GAG'CTC
Cuts at: 1560 1884 2384 1560
Size: 324 500 4548
Fragments arranged by size:
4548 500 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1064 3197 3629 4376 4644 931
Size: 133 2133 432 747 268 1659
Fragments arranged by size:
2133 1659 747 432 268 133
EcoNI CCTnn'n_nnAGG
Cuts at: 1393 1393
Size: 5372
EcoO109I rG'GnC_Cy
Cuts at: 141 332 1253 1299 1512 1653 1654 2022 2151
Size: 191 921 46 213 141 1 368 129
Cuts at: 2151 2190 2511 141
Size: 39 321 3002
Fragments arranged by size:
3002 921 368 321 213 191 141 129
46 39 1
EcoRI G'AATT_C
Cuts at: 2376 2376
Size: 5372
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3391 3391
Size: 5372
FspI TGC'GCA
Cuts at: 388 484 583 3153 388
Size: 96 99 2570 2607
Fragments arranged by size:
2607 2570 99 96
FspAI rTGC'GCAy
Cuts at: 388 388
Size: 5372
HaeII r_GCGC'y
Cuts at: 163 616 1102 1214 1277 1500 1834 1958 2306
Size: 453 486 112 63 223 334 124 348
Cuts at: 2306 2806 3054 4076 5055 163
Size: 500 248 1022 979 480
Fragments arranged by size:
1022 979 500 486 480 453 348 334
248 223 124 112 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 279 311 1303 1335 1873 1905 2224 2256 3214
Size: 32 992 32 538 32 319 32 958
Cuts at: 3214 3244 3246 3276 279
Size: 30 2 30 2375
Fragments arranged by size:
2375 992 958 538 319 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1776 2316 2411 1776
Size: 540 95 4737
Fragments arranged by size:
4737 540 95
KasI G'GCGC_C
Cuts at: 612 1098 1210 1273 1954 2302 2802 3050 612
Size: 486 112 63 681 348 500 248 2934
Fragments arranged by size:
2934 681 500 486 348 248 112 63
KpnI G_GTAC'C
Cuts at: 1811 2392 1811
Size: 581 4791
MluI A'CGCG_T
Cuts at: 1230 1538 4805 1230
Size: 308 3267 1797
Fragments arranged by size:
3267 1797 308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4043 4227 4043
Size: 184 5188
MscI TGG'CCA
Cuts at: 1393 1936 3133 1393
Size: 543 1197 3632
Fragments arranged by size:
3632 1197 543
MslI CAynn'nnrTG
Cuts at: 724 836 3361 3488 724
Size: 112 2525 127 2608
Fragments arranged by size:
2608 2525 127 112
MspA1I CmG'CkG
Cuts at: 504 1629 1638 1729 1793 2483 2797 3157 4170
Size: 1125 9 91 64 690 314 360 1013
Cuts at: 4170 4415 504
Size: 245 1461
Fragments arranged by size:
1461 1125 1013 690 360 314 245 91
64 9
NaeI GCC'GGC
Cuts at: 1097 3553 1097
Size: 2456 2916
NarI GG'CG_CC
Cuts at: 613 1099 1211 1274 1955 2303 2803 3051 613
Size: 486 112 63 681 348 500 248 2934
Fragments arranged by size:
2934 681 500 486 348 248 112 63
NgoMIV G'CCGG_C
Cuts at: 1095 3551 1095
Size: 2456 2916
NspI r_CATG'y
Cuts at: 2425 2925 3832 2425
Size: 500 907 3965
Fragments arranged by size:
3965 907 500
PasI CC'CwG_GG
Cuts at: 1657 2298 1657
Size: 641 4731
PciI A'CATG_T
Cuts at: 3828 3828
Size: 5372
PflMI CCAn_nnn'nTGG
Cuts at: 1389 1974 1389
Size: 585 4787
PmeI GTTT'AAAC
Cuts at: 2371 2371
Size: 5372
PmlI CAC'GTG
Cuts at: 1854 1854
Size: 5372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 4511
Size: 32 5340
PpuMI rG'GwC_Cy
Cuts at: 1512 2190 1512
Size: 678 4694
PspOMI G'GGCC_C
Cuts at: 140 1252 1653 1660 140
Size: 1112 401 7 3852
Fragments arranged by size:
3852 1112 401 7
PspXI vC'TCGA_Gb
Cuts at: 2080 2080
Size: 5372
PstI C_TGCA'G
Cuts at: 503 704 779 2198 2419 3104 503
Size: 201 75 1419 221 685 2771
Fragments arranged by size:
2771 1419 685 221 201 75
PvuI CG_AT'CG
Cuts at: 104 2289 104
Size: 2185 3187
PvuII CAG'CTG
Cuts at: 504 1629 2797 3157 504
Size: 1125 1168 360 2719
Fragments arranged by size:
2719 1168 1125 360
RsrII CG'GwC_CG
Cuts at: 3567 3567
Size: 5372
SacI G_AGCT'C
Cuts at: 1562 1886 2386 1562
Size: 324 500 4548
Fragments arranged by size:
4548 500 324
SacII CC_GC'GG
Cuts at: 1794 1794
Size: 5372
SalI G'TCGA_C
Cuts at: 2409 2409
Size: 5372
SanDI GG'GwC_CC
Cuts at: 1512 1512
Size: 5372
SapI GCTCTTCn'nnn_
Cuts at: 3395 3605 3395
Size: 210 5162
SbfI CC_TGCA'GG
Cuts at: 2419 2419
Size: 5372
ScaI AGT'ACT
Cuts at: 4889 4889
Size: 5372
SfcI C'TryA_G
Cuts at: 33 499 700 775 2194 2343 2415 3100 4093
Size: 466 201 75 1419 149 72 685 993
Cuts at: 4093 4284 33
Size: 191 1121
Fragments arranged by size:
1419 1121 993 685 466 201 191 149
75 72
SfoI GGC'GCC
Cuts at: 614 1100 1212 1275 1956 2304 2804 3052 614
Size: 486 112 63 681 348 500 248 2934
Fragments arranged by size:
2934 681 500 486 348 248 112 63
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
SmaI CCC'GGG
Cuts at: 544 724 1692 1988 2394 544
Size: 180 968 296 406 3522
Fragments arranged by size:
3522 968 406 296 180
SmlI C'TyrA_G
Cuts at: 427 1561 2080 2527 3442 3934 4196 427
Size: 1134 519 447 915 492 262 1603
Fragments arranged by size:
1603 1134 915 519 492 447 262
SnaBI TAC'GTA
Cuts at: 2364 2364
Size: 5372
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5372
SphI G_CATG'C
Cuts at: 2425 2925 2425
Size: 500 4872
StuI AGG'CCT
Cuts at: 706 1388 706
Size: 682 4690
StyI C'CwwG_G
Cuts at: 1166 1328 1383 1394 2243 2506 2860 1166
Size: 162 55 11 849 263 354 3678
Fragments arranged by size:
3678 849 354 263 162 55 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1432 2955 3088 3547 3756 1432
Size: 1523 133 459 209 3048
Fragments arranged by size:
3048 1523 459 209 133
TatI w'GTAC_w
Cuts at: 2279 4887 2279
Size: 2608 2764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1103 1135 4889 4921 1103
Size: 32 3754 32 1554
Fragments arranged by size:
3754 1554 32 32
Tth111I GACn'n_nGTC
Cuts at: 3169 3169
Size: 5372
XbaI T'CTAG_A
Cuts at: 2403 2403
Size: 5372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1088 2332 2867 1088
Size: 1244 535 3593
Fragments arranged by size:
3593 1244 535
XhoI C'TCGA_G
Cuts at: 2080 2080
Size: 5372
XmaI C'CCGG_G
Cuts at: 542 722 1690 1986 2392 542
Size: 180 968 296 406 3522
Fragments arranged by size:
3522 968 406 296 180
ZraI GAC'GTC
Cuts at: 345 345
Size: 5372
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BbvCI BfrBI
BmtI BsrGI BstEII BstXI BstZ17I ClaI EcoRV FseI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PfoI PshAI PsiI PsrI SexAI SfiI
SgrAI SrfI SspI SwaI XmnI