Restriction map for
pF1KSDA1458
(Circular) (Six-base) MAPSORT of: pF1KSDA1458.seq Check: 310 from: 1 to: 4880
pF1KSDA1458 4880 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1130 1918 1130
Size: 788 4092
Acc65I G'GTAC_C
Cuts at: 1896 1896
Size: 4880
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1463 2705 3137 3884 4152 884
Size: 579 1242 432 747 268 1612
Fragments arranged by size:
1612 1242 747 579 432 268
AfeI AGC'GCT
Cuts at: 1686 1686
Size: 4880
AflIII A'CryG_T
Cuts at: 2232 3336 4313 2232
Size: 1104 977 2799
Fragments arranged by size:
2799 1104 977
AgeI A'CCGG_T
Cuts at: 3268 3268
Size: 4880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1019 1786 1825 1019
Size: 767 39 4074
Fragments arranged by size:
4074 767 39
AlwNI CAG_nnn'CTG
Cuts at: 183 2185 3752 183
Size: 2002 1567 1311
Fragments arranged by size:
2002 1567 1311
ApaI G_GGCC'C
Cuts at: 263 362 373 263
Size: 99 11 4770
Fragments arranged by size:
4770 99 11
ApaLI G'TGCA_C
Cuts at: 3650 3650
Size: 4880
ApoI r'AATT_y
Cuts at: 678 822 1203 1431 1501 1884 4233 4489 678
Size: 144 381 228 70 383 2349 256 1069
Fragments arranged by size:
2349 1069 383 381 256 228 144 70
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 228 317 361 372 1229 1900 228
Size: 89 44 11 857 671 3208
Fragments arranged by size:
3208 857 671 89 44 11
BamHI G'GATC_C
Cuts at: 925 1905 925
Size: 980 3900
BanI G'GyrC_C
Cuts at: 244 1896 2310 2558 2593 244
Size: 1652 414 248 35 2531
Fragments arranged by size:
2531 1652 414 248 35
BanII G_rGCy'C
Cuts at: 263 362 373 1894 2924 263
Size: 99 11 1521 1030 2219
Fragments arranged by size:
2219 1521 1030 99 11
BbeI G_GCGC'C
Cuts at: 248 2314 2562 248
Size: 2066 248 2566
Fragments arranged by size:
2566 2066 248
BbsI GAAGACnn'nnnn_
Cuts at: 1357 1357
Size: 4880
BbvCI CC'TCA_GC
Cuts at: 217 337 217
Size: 120 4760
BciVI GTATCCnnnnn_n'
Cuts at: 2778 3539 4880 2778
Size: 761 1341 2778
Fragments arranged by size:
2778 1341 761
BclI T'GATC_A
Cuts at: 648 1071 2400 648
Size: 423 1329 3128
Fragments arranged by size:
3128 1329 423
BfrBI ATG'CAT
Cuts at: 1119 1119
Size: 4880
BglI GCCn_nnn'nGGC
Cuts at: 462 488 462
Size: 26 4854
BglII A'GATC_T
Cuts at: 2395 4432 2395
Size: 2037 2843
BlpI GC'TnA_GC
Cuts at: 1992 1992
Size: 4880
Bme1580I G_kGCm'C
Cuts at: 263 362 373 1749 2505 2598 3654 263
Size: 99 11 1376 756 93 1056 1489
Fragments arranged by size:
1489 1376 1056 756 99 93 11
BmgBI CAC'GTC
Cuts at: 1016 1016
Size: 4880
BmrI ACTGGGnnnn_n'
Cuts at: 775 1736 2092 2173 2508 4652 775
Size: 961 356 81 335 2144 1003
Fragments arranged by size:
2144 1003 961 356 335 81
BmtI G_CTAG'C
Cuts at: 421 421
Size: 4880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 4880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 496 1478 217
Size: 279 982 3619
Fragments arranged by size:
3619 982 279
Bpu10I CC'TnA_GC
Cuts at: 217 337 1187 217
Size: 120 850 3910
Fragments arranged by size:
3910 850 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 2056 2935 3427 3725 652
Size: 1404 879 492 298 1807
Fragments arranged by size:
1807 1404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4584 4584
Size: 4880
BsaAI yAC'GTr
Cuts at: 1872 2233 1872
Size: 361 4519
BsaBI GATnn'nnATC
Cuts at: 6 2394 4431 6
Size: 2388 2037 455
Fragments arranged by size:
2388 2037 455
BsaHI Gr'CG_yC
Cuts at: 245 310 404 2311 2559 3261 4757 245
Size: 65 94 1907 248 702 1496 368
Fragments arranged by size:
1907 1496 702 368 248 94 65
BsaWI w'CCGG_w
Cuts at: 2069 2590 3268 3542 3689 4100 2069
Size: 521 678 274 147 411 2849
Fragments arranged by size:
2849 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 124 154 394 424 469 499 124
Size: 30 240 30 45 30 4505
Fragments arranged by size:
4505 240 45 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 234 288 305 511 514 517 520 1611 234
Size: 54 17 206 3 3 3 1091 3503
Fragments arranged by size:
3503 1091 206 54 17 3 3 3
BseYI C'CCAG_C
Cuts at: 491 1669 1756 2305 3063 3640 4136 491
Size: 1178 87 549 758 577 496 1235
Fragments arranged by size:
1235 1178 758 577 549 496 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 600 259
Size: 341 4539
BsiEI CG_ry'CG
Cuts at: 104 1180 2422 2468 3676 104
Size: 1076 1242 46 1208 1308
Fragments arranged by size:
1308 1242 1208 1076 46
BsiHKAI G_wGCw'C
Cuts at: 1651 1894 2672 2862 3654 1651
Size: 243 778 190 792 2877
Fragments arranged by size:
2877 792 778 243 190
BsmBI CGTCTCn'nnnn_
Cuts at: 317 1022 317
Size: 705 4175
Bsp1286I G_dGCh'C
Cuts at: 263 362 373 1651 1749 1894 2505 2598 2672
Size: 99 11 1278 98 145 611 93 74
Cuts at: 2672 2862 2924 3654 263
Size: 190 62 730 1489
Fragments arranged by size:
1489 1278 730 611 190 145 99 98
93 74 62 11
BspEI T'CCGG_A
Cuts at: 4100 4100
Size: 4880
BspHI T'CATG_A
Cuts at: 4056 4056
Size: 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1710 1930 2144 2446 2827 1710
Size: 220 214 302 381 3763
Fragments arranged by size:
3763 381 302 220 214
BsrBI CCG'CTC
Cuts at: 50 227 3172 3226 4710 4875 50
Size: 177 2945 54 1484 165 55
Fragments arranged by size:
2945 1484 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1338 1724 2792 1338
Size: 386 1068 3426
Fragments arranged by size:
3426 1068 386
BsrFI r'CCGG_y
Cuts at: 247 280 2878 3059 3268 247
Size: 33 2598 181 209 1859
Fragments arranged by size:
2598 1859 209 181 33
BsrGI T'GTAC_A
Cuts at: 957 957
Size: 4880
BssSI C'ACGA_G
Cuts at: 3151 3509 3151
Size: 358 4522
BstAPI GCAn_nnn'nTGC
Cuts at: 2257 2257
Size: 4880
BstBI TT'CG_AA
Cuts at: 110 3241 110
Size: 3131 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 575 1573 575
Size: 998 3882
BstYI r'GATC_y
Cuts at: 1 925 1804 1905 2372 2395 2730 2976 3988
Size: 924 879 101 467 23 335 246 1012
Cuts at: 3988 4074 4086 4432 1
Size: 86 12 346 449
Fragments arranged by size:
1012 924 879 467 449 346 335 246
101 86 23 12
BstZ17I GTA'TAC
Cuts at: 1131 1131
Size: 4880
Bsu36I CC'TnA_GG
Cuts at: 1472 1472
Size: 4880
BtgI C'CryG_G
Cuts at: 125 1566 125
Size: 1441 3439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2830 3011 3174 2830
Size: 181 163 4536
Fragments arranged by size:
4536 181 163
BtsI GCAGTG_nn'
Cuts at: 759 2123 759
Size: 1364 3516
DraI TTT'AAA
Cuts at: 1879 1879
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 2586 3444 2586
Size: 858 4022
EaeI y'GGCC_r
Cuts at: 2465 2639 3030 3057 4402 2465
Size: 174 391 27 1345 2943
Fragments arranged by size:
2943 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2465 2465
Size: 4880
EarI CTCTTCn'nnn_
Cuts at: 386 1541 2903 3113 386
Size: 1155 1362 210 2153
Fragments arranged by size:
2153 1362 1155 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 393 3088 3398 3544 4690 393
Size: 2695 310 146 1146 583
Fragments arranged by size:
2695 1146 583 310 146
EcoICRI GAG'CTC
Cuts at: 1892 1892
Size: 4880
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 496 884 1463 1478 2705 3137 3884 4152
Size: 279 388 579 15 1227 432 747 268
Cuts at: 4152 217
Size: 945
Fragments arranged by size:
1227 945 747 579 432 388 279 268
15
EcoNI CCTnn'n_nnAGG
Cuts at: 1493 1493
Size: 4880
EcoO109I rG'GnC_Cy
Cuts at: 2019 2019
Size: 4880
EcoRI G'AATT_C
Cuts at: 1884 1884
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 4880
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 248 344 483 1688 2314 2562 3584 4563 248
Size: 96 139 1205 626 248 1022 979 565
Fragments arranged by size:
1205 1022 979 626 565 248 139 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 394 426 469 501 2722 2752 2754
Size: 32 238 32 43 32 2221 30 2
Cuts at: 2754 2784 124
Size: 30 2220
Fragments arranged by size:
2221 2220 238 43 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 138 1143 1401 1919 138
Size: 1005 258 518 3099
Fragments arranged by size:
3099 1005 518 258
HpaI GTT'AAC
Cuts at: 138 1143 138
Size: 1005 3875
KasI G'GCGC_C
Cuts at: 244 2310 2558 244
Size: 2066 248 2566
Fragments arranged by size:
2566 2066 248
KpnI G_GTAC'C
Cuts at: 1900 1900
Size: 4880
MfeI C'AATT_G
Cuts at: 920 920
Size: 4880
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 1831 3551 3735 167
Size: 1664 1720 184 1312
Fragments arranged by size:
1720 1664 1312 184
MscI TGG'CCA
Cuts at: 2641 2641
Size: 4880
MslI CAynn'nnrTG
Cuts at: 1768 2869 2996 1768
Size: 1101 127 3652
Fragments arranged by size:
3652 1101 127
MspA1I CmG'CkG
Cuts at: 216 459 1991 2305 2665 3678 3923 216
Size: 243 1532 314 360 1013 245 1173
Fragments arranged by size:
1532 1173 1013 360 314 245 243
NaeI GCC'GGC
Cuts at: 249 282 3061 249
Size: 33 2779 2068
Fragments arranged by size:
2779 2068 33
NarI GG'CG_CC
Cuts at: 245 2311 2559 245
Size: 2066 248 2566
Fragments arranged by size:
2566 2066 248
NcoI C'CATG_G
Cuts at: 125 1566 125
Size: 1441 3439
NgoMIV G'CCGG_C
Cuts at: 247 280 3059 247
Size: 33 2779 2068
Fragments arranged by size:
2779 2068 33
NheI G'CTAG_C
Cuts at: 417 417
Size: 4880
NsiI A_TGCA'T
Cuts at: 1121 1121
Size: 4880
NspI r_CATG'y
Cuts at: 1933 2433 3340 1933
Size: 500 907 3473
Fragments arranged by size:
3473 907 500
PciI A'CATG_T
Cuts at: 3336 3336
Size: 4880
PfoI T'CCnGG_A
Cuts at: 816 816
Size: 4880
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4848
PsiI TTA'TAA
Cuts at: 935 935
Size: 4880
PspOMI G'GGCC_C
Cuts at: 259 358 369 259
Size: 99 11 4770
Fragments arranged by size:
4770 99 11
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1374 1406 1374
Size: 32 4848
PstI C_TGCA'G
Cuts at: 179 1518 1707 1927 2612 179
Size: 1339 189 220 685 2447
Fragments arranged by size:
2447 1339 685 220 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 216 2305 2665 216
Size: 2089 360 2431
Fragments arranged by size:
2431 2089 360
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 1894 1894
Size: 4880
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 386 1541 2903 3113 386
Size: 1155 1362 210 2153
Fragments arranged by size:
2153 1362 1155 210
SbfI CC_TGCA'GG
Cuts at: 1707 1927 1707
Size: 220 4660
ScaI AGT'ACT
Cuts at: 4397 4397
Size: 4880
SfcI C'TryA_G
Cuts at: 33 175 794 878 1514 1703 1923 2608 3601
Size: 142 619 84 636 189 220 685 993
Cuts at: 3601 3792 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 636 619 220 191 189
142 84
SfoI GGC'GCC
Cuts at: 246 2312 2560 246
Size: 2066 248 2566
Fragments arranged by size:
2566 2066 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 363 1902 363
Size: 1539 3341
SmlI C'TyrA_G
Cuts at: 667 2035 2950 3442 3704 667
Size: 1368 915 492 262 1843
Fragments arranged by size:
1843 1368 915 492 262
SnaBI TAC'GTA
Cuts at: 1872 1872
Size: 4880
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1933 2433 1933
Size: 500 4380
StyI C'CwwG_G
Cuts at: 125 255 329 1566 2014 2368 125
Size: 130 74 1237 448 354 2637
Fragments arranged by size:
2637 1237 448 354 130 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 2463 2596 3055 3264 1212
Size: 1251 133 459 209 2828
Fragments arranged by size:
2828 1251 459 209 133
TatI w'GTAC_w
Cuts at: 957 4395 957
Size: 3438 1442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4397 4429 4397
Size: 32 4848
Tth111I GACn'n_nGTC
Cuts at: 1361 2677 1361
Size: 1316 3564
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2375 2375
Size: 4880
XmaI C'CCGG_G
Cuts at: 361 1900 361
Size: 1539 3341
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PciI PfoI PmeI PpiI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BsiWI BsmI BssHII BstEII ClaI CspCI
DraIII EcoRV FseI FspAI HindIII NdeI NotI NruI
PacI PasI PflMI PmlI PpuMI PshAI PspXI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI