Restriction map for
pF1KSDA1445
(Circular) (Six-base) MAPSORT of: pF1KSDA1445.seq Check: 9312 from: 1 to: 6590
pF1KSDA1445 6590 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2536 2536
Size: 6590
AccI GT'mk_AC
Cuts at: 3628 3628
Size: 6590
Acc65I G'GTAC_C
Cuts at: 3606 3606
Size: 6590
AclI AA'CG_TT
Cuts at: 1866 6430 1866
Size: 4564 2026
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 549 580 1642 4415 4847 5594 5862 123
Size: 426 31 1062 2773 432 747 268 851
Fragments arranged by size:
2773 1062 851 747 432 426 268 31
AflIII A'CryG_T
Cuts at: 3942 5046 6023 3942
Size: 1104 977 4509
Fragments arranged by size:
4509 1104 977
AgeI A'CCGG_T
Cuts at: 1417 4978 1417
Size: 3561 3029
AleI CACnn'nnGTG
Cuts at: 1052 1052
Size: 6590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2242 2274 2368 2400 2500 2532 2242
Size: 32 94 32 100 32 6300
Fragments arranged by size:
6300 100 94 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 414 2336 3119 3895 5462 414
Size: 1922 783 776 1567 1542
Fragments arranged by size:
1922 1567 1542 783 776
ApaI G_GGCC'C
Cuts at: 1830 2707 1830
Size: 877 5713
ApaLI G'TGCA_C
Cuts at: 747 2763 3054 5360 747
Size: 2016 291 2306 1977
Fragments arranged by size:
2306 2016 1977 291
ApoI r'AATT_y
Cuts at: 3415 3594 5943 6199 3415
Size: 179 2349 256 3806
Fragments arranged by size:
3806 2349 256 179
AseI AT'TA_AT
Cuts at: 20 20
Size: 6590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
AvaI C'yCGr_G
Cuts at: 494 1170 1504 1690 1829 1879 2425 2723 2974
Size: 676 334 186 139 50 546 298 251
Cuts at: 2974 3541 3610 494
Size: 567 69 3474
Fragments arranged by size:
3474 676 567 546 334 298 251 186
139 69 50
BamHI G'GATC_C
Cuts at: 3615 3615
Size: 6590
BanI G'GyrC_C
Cuts at: 1402 1600 1612 1694 2286 3382 3606 4020 4268
Size: 198 12 82 592 1096 224 414 248
Cuts at: 4268 4303 1402
Size: 35 3689
Fragments arranged by size:
3689 1096 592 414 248 224 198 82
35 12
BanII G_rGCy'C
Cuts at: 398 495 866 1511 1706 1830 2034 2314 2707
Size: 97 371 645 195 124 204 280 393
Cuts at: 2707 2780 3072 3101 3268 3275 3604 4634 398
Size: 73 292 29 167 7 329 1030 2354
Fragments arranged by size:
2354 1030 645 393 371 329 292 280
204 195 167 124 97 73 29 7
BbeI G_GCGC'C
Cuts at: 1698 4024 4272 1698
Size: 2326 248 4016
Fragments arranged by size:
4016 2326 248
BbsI GAAGACnn'nnnn_
Cuts at: 244 458 661 244
Size: 214 203 6173
Fragments arranged by size:
6173 214 203
BbvCI CC'TCA_GC
Cuts at: 772 772
Size: 6590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1860 1894 1860
Size: 34 6556
BciVI GTATCCnnnnn_n'
Cuts at: 4488 5249 6590 4488
Size: 761 1341 4488
Fragments arranged by size:
4488 1341 761
BclI T'GATC_A
Cuts at: 4110 4110
Size: 6590
BglI GCCn_nnn'nGGC
Cuts at: 1655 1692 2222 3010 3023 1655
Size: 37 530 788 13 5222
Fragments arranged by size:
5222 788 530 37 13
BglII A'GATC_T
Cuts at: 4105 6142 4105
Size: 2037 4553
BlpI GC'TnA_GC
Cuts at: 2892 3702 2892
Size: 810 5780
Bme1580I G_kGCm'C
Cuts at: 751 1615 1830 2291 2502 2707 2767 3058 4215
Size: 864 215 461 211 205 60 291 1157
Cuts at: 4215 4308 5364 751
Size: 93 1056 1977
Fragments arranged by size:
1977 1157 1056 864 461 291 215 211
205 93 60
BmgBI CAC'GTC
Cuts at: 1101 2159 2417 1101
Size: 1058 258 5274
Fragments arranged by size:
5274 1058 258
BmrI ACTGGGnnnn_n'
Cuts at: 2842 3802 3883 4218 6362 2842
Size: 960 81 335 2144 3070
Fragments arranged by size:
3070 2144 960 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 630 1905 4957 630
Size: 1275 3052 2263
Fragments arranged by size:
3052 2263 1275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 372 509 600 1141 1687 1804 2094 2337
Size: 18 137 91 541 546 117 290 243
Cuts at: 2337 3171 354
Size: 834 3773
Fragments arranged by size:
3773 834 546 541 290 243 137 117
91 18
Bpu10I CC'TnA_GC
Cuts at: 202 424 772 202
Size: 222 348 6020
Fragments arranged by size:
6020 348 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4645 5137 5435 3766
Size: 879 492 298 4921
Fragments arranged by size:
4921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 474 1408 2888 6294 474
Size: 934 1480 3406 770
Fragments arranged by size:
3406 1480 934 770
BsaAI yAC'GTr
Cuts at: 681 1680 3582 3943 681
Size: 999 1902 361 3328
Fragments arranged by size:
3328 1902 999 361
BsaBI GATnn'nnATC
Cuts at: 6 4104 6141 6
Size: 4098 2037 455
Fragments arranged by size:
4098 2037 455
BsaHI Gr'CG_yC
Cuts at: 1695 1766 2361 2464 2630 4021 4269 4971 6467
Size: 71 595 103 166 1391 248 702 1496
Cuts at: 6467 1695
Size: 1818
Fragments arranged by size:
1818 1496 1391 702 595 248 166 103
71
BsaWI w'CCGG_w
Cuts at: 1417 3779 4300 4978 5252 5399 5810 1417
Size: 2362 521 678 274 147 411 2197
Fragments arranged by size:
2362 2197 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2242 2272 2368 2398 2500 2530 2242
Size: 30 96 30 102 30 6302
Fragments arranged by size:
6302 102 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 676 679 840 1684 3004 3091 3109 3211 676
Size: 3 161 844 1320 87 18 102 4055
Fragments arranged by size:
4055 1320 844 161 102 87 18 3
BseYI C'CCAG_C
Cuts at: 189 340 397 506 1847 2690 2736 4015 4773
Size: 151 57 109 1341 843 46 1279 758
Cuts at: 4773 5350 5846 189
Size: 577 496 933
Fragments arranged by size:
1341 1279 933 843 758 577 496 151
109 57 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 1582 1660 1711 2172 2346 2919 2965 3233
Size: 72 78 51 461 174 573 46 268
Cuts at: 3233 1510
Size: 4867
Fragments arranged by size:
4867 573 461 268 174 78 72 51
46
BsiEI CG_ry'CG
Cuts at: 104 705 4132 4178 5386 104
Size: 601 3427 46 1208 1308
Fragments arranged by size:
3427 1308 1208 601 46
BsiHKAI G_wGCw'C
Cuts at: 751 866 1049 1994 2034 2665 2767 3058 3101
Size: 115 183 945 40 631 102 291 43
Cuts at: 3101 3604 4382 4572 5364 751
Size: 503 778 190 792 1977
Fragments arranged by size:
1977 945 792 778 631 503 291 190
183 115 102 43 40
BsmBI CGTCTCn'nnnn_
Cuts at: 152 1754 2578 152
Size: 1602 824 4164
Fragments arranged by size:
4164 1602 824
Bsp1286I G_dGCh'C
Cuts at: 398 495 751 866 1049 1511 1615 1706 1830
Size: 97 256 115 183 462 104 91 124
Cuts at: 1830 1994 2034 2291 2314 2502 2665 2707 2767
Size: 164 40 257 23 188 163 42 60
Cuts at: 2767 2780 3058 3072 3101 3268 3275 3604 4215
Size: 13 278 14 29 167 7 329 611
Cuts at: 4215 4308 4382 4572 4634 5364 398
Size: 93 74 190 62 730 1624
Fragments arranged by size:
1624 730 611 462 329 278 257 256
190 188 183 167 164 163 124 115
104 97 93 91 74 62 60 42
40 29 23 14 13 7
BspEI T'CCGG_A
Cuts at: 5810 5810
Size: 6590
BspHI T'CATG_A
Cuts at: 1142 5766 1142
Size: 4624 1966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 2536 3640 3854 4156 4537 464
Size: 2072 1104 214 302 381 2517
Fragments arranged by size:
2517 2072 1104 381 302 214
BsrBI CCG'CTC
Cuts at: 50 635 2684 4882 4936 6420 6585 50
Size: 585 2049 2198 54 1484 165 55
Fragments arranged by size:
2198 2049 1484 585 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3472 4502 3472
Size: 1030 5560
BsrFI r'CCGG_y
Cuts at: 700 1417 1494 3083 3205 4588 4769 4978 700
Size: 717 77 1589 122 1383 181 209 2312
Fragments arranged by size:
2312 1589 1383 717 209 181 122 77
BsrGI T'GTAC_A
Cuts at: 3491 3491
Size: 6590
BssHII G'CGCG_C
Cuts at: 1739 2534 1739
Size: 795 5795
BssSI C'ACGA_G
Cuts at: 1546 4861 5219 1546
Size: 3315 358 2917
Fragments arranged by size:
3315 2917 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1683 2387 2559 3001 3254 3967 1683
Size: 704 172 442 253 713 4306
Fragments arranged by size:
4306 713 704 442 253 172
BstBI TT'CG_AA
Cuts at: 110 4951 110
Size: 4841 1749
BstEII G'GTnAC_C
Cuts at: 1970 1970
Size: 6590
BstXI CCAn_nnnn'nTGG
Cuts at: 3026 3026
Size: 6590
BstYI r'GATC_y
Cuts at: 1 3615 4082 4105 4440 4686 5698 5784 5796
Size: 3614 467 23 335 246 1012 86 12
Cuts at: 5796 6142 1
Size: 346 449
Fragments arranged by size:
3614 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 440 463 875 1634 1653 1966 1975 2131 2217
Size: 23 412 759 19 313 9 156 86
Cuts at: 2217 2467 2600 2707 3242 3410 440
Size: 250 133 107 535 168 3620
Fragments arranged by size:
3620 759 535 412 313 250 168 156
133 107 86 23 19 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1254 2082 2822 4540 4721 4884 1254
Size: 828 740 1718 181 163 2960
Fragments arranged by size:
2960 1718 828 740 181 163
BtsI GCAGTG_nn'
Cuts at: 349 408 933 944 1046 3293 3306 3833 349
Size: 59 525 11 102 2247 13 527 3106
Fragments arranged by size:
3106 2247 527 525 102 59 13 11
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 968 1003 968
Size: 35 6555
DraI TTT'AAA
Cuts at: 3589 3589
Size: 6590
DraIII CAC_nnn'GTG
Cuts at: 608 1548 2679 3001 3237 608
Size: 940 1131 322 236 3961
Fragments arranged by size:
3961 1131 940 322 236
DrdI GACnn_nn'nnGTC
Cuts at: 2133 2880 4296 5154 2133
Size: 747 1416 858 3569
Fragments arranged by size:
3569 1416 858 747
EaeI y'GGCC_r
Cuts at: 702 3239 4175 4349 4740 4767 6112 702
Size: 2537 936 174 391 27 1345 1180
Fragments arranged by size:
2537 1345 1180 936 391 174 27
EagI C'GGCC_G
Cuts at: 702 4175 702
Size: 3473 3117
EarI CTCTTCn'nnn_
Cuts at: 568 596 1474 1750 4613 4823 568
Size: 28 878 276 2863 210 2335
Fragments arranged by size:
2863 2335 878 276 210 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2173 3392 4798 5108 5254 6400 2173
Size: 1219 1406 310 146 1146 2363
Fragments arranged by size:
2363 1406 1219 1146 310 146
EcoICRI GAG'CTC
Cuts at: 864 2032 3099 3602 864
Size: 1168 1067 503 3852
Fragments arranged by size:
3852 1168 1067 503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 354 372 509 549 580 600 1141 1642
Size: 231 18 137 40 31 20 541 501
Cuts at: 1642 1687 1804 2094 2337 3171 4415 4847 5594
Size: 45 117 290 243 834 1244 432 747
Cuts at: 5594 5862 123
Size: 268 851
Fragments arranged by size:
1244 851 834 747 541 501 432 290
268 243 231 137 117 45 40 31
20 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1120 1561 2638 1120
Size: 441 1077 5072
Fragments arranged by size:
5072 1077 441
EcoO109I rG'GnC_Cy
Cuts at: 292 313 900 1179 1230 1776 1826 1833 2067
Size: 21 587 279 51 546 50 7 234
Cuts at: 2067 2591 2643 2703 2790 3729 292
Size: 524 52 60 87 939 3153
Fragments arranged by size:
3153 939 587 546 524 279 234 87
60 52 51 50 21 7
EcoRI G'AATT_C
Cuts at: 3415 3594 3415
Size: 179 6411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3456 4609 3456
Size: 1153 5437
FspI TGC'GCA
Cuts at: 1711 1720 2653 4371 1711
Size: 9 933 1718 3930
Fragments arranged by size:
3930 1718 933 9
HaeII r_GCGC'y
Cuts at: 1467 1630 1698 1736 2149 3119 4024 4272 5294
Size: 163 68 38 413 970 905 248 1022
Cuts at: 5294 6273 1467
Size: 979 1784
Fragments arranged by size:
1784 1022 979 970 905 413 248 163
68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 677 709 1751 1783 2580 2612 4432 4462 4464
Size: 32 1042 32 797 32 1820 30 2
Cuts at: 4464 4494 677
Size: 30 2773
Fragments arranged by size:
2773 1820 1042 797 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3629 3629
Size: 6590
KasI G'GCGC_C
Cuts at: 1694 4020 4268 1694
Size: 2326 248 4016
Fragments arranged by size:
4016 2326 248
KpnI G_GTAC'C
Cuts at: 3610 3610
Size: 6590
MluI A'CGCG_T
Cuts at: 6023 6023
Size: 6590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1916 5261 5445 1916
Size: 3345 184 3061
Fragments arranged by size:
3345 3061 184
MscI TGG'CCA
Cuts at: 3241 4351 3241
Size: 1110 5480
MslI CAynn'nnrTG
Cuts at: 1052 1141 1997 2233 3409 4579 4706 1052
Size: 89 856 236 1176 1170 127 2936
Fragments arranged by size:
2936 1176 1170 856 236 127 89
MspA1I CmG'CkG
Cuts at: 414 510 809 841 2060 2514 2602 2657 3701
Size: 96 299 32 1219 454 88 55 1044
Cuts at: 3701 4015 4375 5388 5633 414
Size: 314 360 1013 245 1371
Fragments arranged by size:
1371 1219 1044 1013 454 360 314 299
245 96 88 55 32
NaeI GCC'GGC
Cuts at: 702 3085 4771 702
Size: 2383 1686 2521
Fragments arranged by size:
2521 2383 1686
NarI GG'CG_CC
Cuts at: 1695 4021 4269 1695
Size: 2326 248 4016
Fragments arranged by size:
4016 2326 248
NcoI C'CATG_G
Cuts at: 1966 1975 3410 1966
Size: 9 1435 5146
Fragments arranged by size:
5146 1435 9
NgoMIV G'CCGG_C
Cuts at: 700 3083 4769 700
Size: 2383 1686 2521
Fragments arranged by size:
2521 2383 1686
NspI r_CATG'y
Cuts at: 1820 2352 3643 4143 5050 1820
Size: 532 1291 500 907 3360
Fragments arranged by size:
3360 1291 907 532 500
PasI CC'CwG_GG
Cuts at: 172 855 1352 3106 172
Size: 683 497 1754 3656
Fragments arranged by size:
3656 1754 683 497
PciI A'CATG_T
Cuts at: 5046 5046
Size: 6590
PflMI CCAn_nnn'nTGG
Cuts at: 608 2297 3237 608
Size: 1689 940 3961
Fragments arranged by size:
3961 1689 940
PfoI T'CCnGG_A
Cuts at: 270 526 2110 2314 270
Size: 256 1584 204 4546
Fragments arranged by size:
4546 1584 256 204
PmeI GTTT'AAAC
Cuts at: 3589 3589
Size: 6590
PmlI CAC'GTG
Cuts at: 1680 1680
Size: 6590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2274 2368 2400 2500 2532 5729 5761 2242
Size: 32 94 32 100 32 3197 32 3071
Fragments arranged by size:
3197 3071 100 94 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 900 1179 1230 1776 1833 2591 2643 900
Size: 279 51 546 57 758 52 4847
Fragments arranged by size:
4847 758 546 279 57 52 51
PshAI GACnn'nnGTC
Cuts at: 2969 2969
Size: 6590
PspOMI G'GGCC_C
Cuts at: 1826 2703 1826
Size: 877 5713
PspXI vC'TCGA_Gb
Cuts at: 1879 1879
Size: 6590
PstI C_TGCA'G
Cuts at: 288 461 1205 1454 2564 3637 4322 288
Size: 173 744 249 1110 1073 685 2556
Fragments arranged by size:
2556 1110 1073 744 685 249 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6590
PvuII CAG'CTG
Cuts at: 414 510 841 4015 4375 414
Size: 96 331 3174 360 2629
Fragments arranged by size:
3174 2629 360 331 96
RsrII CG'GwC_CG
Cuts at: 4785 4785
Size: 6590
SacI G_AGCT'C
Cuts at: 866 2034 3101 3604 866
Size: 1168 1067 503 3852
Fragments arranged by size:
3852 1168 1067 503
SacII CC_GC'GG
Cuts at: 2603 2603
Size: 6590
SalI G'TCGA_C
Cuts at: 3627 3627
Size: 6590
SanDI GG'GwC_CC
Cuts at: 900 1776 1833 900
Size: 876 57 5657
Fragments arranged by size:
5657 876 57
SapI GCTCTTCn'nnn_
Cuts at: 1750 4613 4823 1750
Size: 2863 210 3517
Fragments arranged by size:
3517 2863 210
SbfI CC_TGCA'GG
Cuts at: 1454 3637 1454
Size: 2183 4407
ScaI AGT'ACT
Cuts at: 6107 6107
Size: 6590
SexAI A'CCwGG_T
Cuts at: 1556 1556
Size: 6590
SfcI C'TryA_G
Cuts at: 33 284 457 1201 1450 2560 3156 3633 4318
Size: 251 173 744 249 1110 596 477 685
Cuts at: 4318 5311 5502 33
Size: 993 191 1121
Fragments arranged by size:
1121 1110 993 744 685 596 477 251
249 191 173
SfoI GGC'GCC
Cuts at: 1696 4022 4270 1696
Size: 2326 248 4016
Fragments arranged by size:
4016 2326 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
SmaI CCC'GGG
Cuts at: 496 1172 1692 1831 2725 3612 496
Size: 676 520 139 894 887 3474
Fragments arranged by size:
3474 894 887 676 520 139
SmlI C'TyrA_G
Cuts at: 1879 3745 4660 5152 5414 1879
Size: 1866 915 492 262 3055
Fragments arranged by size:
3055 1866 915 492 262
SnaBI TAC'GTA
Cuts at: 3582 3582
Size: 6590
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6590
SphI G_CATG'C
Cuts at: 1820 2352 3643 4143 1820
Size: 532 1291 500 4267
Fragments arranged by size:
4267 1291 532 500
SrfI GCCC'GGGC
Cuts at: 1172 1172
Size: 6590
StuI AGG'CCT
Cuts at: 3013 3547 3013
Size: 534 6056
StyI C'CwwG_G
Cuts at: 1966 1975 3410 3724 4078 1966
Size: 9 1435 314 354 4478
Fragments arranged by size:
4478 1435 354 314 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1534 2596 3423 4173 4306 4765 4974 1534
Size: 1062 827 750 133 459 209 3150
Fragments arranged by size:
3150 1062 827 750 459 209 133
TatI w'GTAC_w
Cuts at: 1067 1587 3401 3491 6105 1067
Size: 520 1814 90 2614 1552
Fragments arranged by size:
2614 1814 1552 520 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1049 1081 1911 1943 6107 6139 1049
Size: 32 830 32 4164 32 1500
Fragments arranged by size:
4164 1500 830 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 4387 1765
Size: 2622 3968
XbaI T'CTAG_A
Cuts at: 3621 3621
Size: 6590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 169 759 1321 1973 4085 169
Size: 590 562 652 2112 2674
Fragments arranged by size:
2674 2112 652 590 562
XhoI C'TCGA_G
Cuts at: 1879 1879
Size: 6590
XmaI C'CCGG_G
Cuts at: 494 1170 1690 1829 2723 3610 494
Size: 676 520 139 894 887 3474
Fragments arranged by size:
3474 894 887 676 520 139
XmnI GAAnn'nnTTC
Cuts at: 712 712
Size: 6590
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PsiI PsrI SfiI SgrAI SspI
SwaI ZraI