Restriction map for
pF1KSDA1431
(Circular) (Six-base) MAPSORT of: pF1KSDA1431.seq Check: 9200 from: 1 to: 5741
pF1KSDA1431 5741 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2779 2779
Size: 5741
Acc65I G'GTAC_C
Cuts at: 2757 2757
Size: 5741
AclI AA'CG_TT
Cuts at: 5581 5581
Size: 5741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1100 1650 1734 1818 1986 2154 2574 3566 3998
Size: 550 84 84 168 168 420 992 432
Cuts at: 3998 4745 5013 1100
Size: 747 268 1828
Fragments arranged by size:
1828 992 747 550 432 420 268 168
168 84 84
AflIII A'CryG_T
Cuts at: 3093 4197 5174 3093
Size: 1104 977 3660
Fragments arranged by size:
3660 1104 977
AgeI A'CCGG_T
Cuts at: 4129 4129
Size: 5741
AleI CACnn'nnGTG
Cuts at: 2213 2213
Size: 5741
AlwNI CAG_nnn'CTG
Cuts at: 3046 4613 3046
Size: 1567 4174
ApaLI G'TGCA_C
Cuts at: 4511 4511
Size: 5741
ApoI r'AATT_y
Cuts at: 876 1323 1445 1700 2624 2695 2745 5094 5350
Size: 447 122 255 924 71 50 2349 256
Cuts at: 5350 876
Size: 1267
Fragments arranged by size:
2349 1267 924 447 256 255 122 71
50
AseI AT'TA_AT
Cuts at: 20 20
Size: 5741
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5741
AvaI C'yCGr_G
Cuts at: 171 200 254 2761 171
Size: 29 54 2507 3151
Fragments arranged by size:
3151 2507 54 29
BamHI G'GATC_C
Cuts at: 2766 2766
Size: 5741
BanI G'GyrC_C
Cuts at: 181 641 2757 3171 3419 3454 181
Size: 460 2116 414 248 35 2468
Fragments arranged by size:
2468 2116 460 414 248 35
BanII G_rGCy'C
Cuts at: 336 983 1423 2755 3785 336
Size: 647 440 1332 1030 2292
Fragments arranged by size:
2292 1332 1030 647 440
BbeI G_GCGC'C
Cuts at: 185 3175 3423 185
Size: 2990 248 2503
Fragments arranged by size:
2990 2503 248
BbsI GAAGACnn'nnnn_
Cuts at: 2580 2580
Size: 5741
BciVI GTATCCnnnnn_n'
Cuts at: 3639 4400 5741 3639
Size: 761 1341 3639
Fragments arranged by size:
3639 1341 761
BclI T'GATC_A
Cuts at: 3261 3261
Size: 5741
BfrBI ATG'CAT
Cuts at: 1846 1846
Size: 5741
BglI GCCn_nnn'nGGC
Cuts at: 241 241
Size: 5741
BglII A'GATC_T
Cuts at: 2557 3256 5293 2557
Size: 699 2037 3005
Fragments arranged by size:
3005 2037 699
BlpI GC'TnA_GC
Cuts at: 941 2853 941
Size: 1912 3829
Bme1580I G_kGCm'C
Cuts at: 329 646 3366 3459 4515 329
Size: 317 2720 93 1056 1555
Fragments arranged by size:
2720 1555 1056 317 93
BmgBI CAC'GTC
Cuts at: 2669 2669
Size: 5741
BmrI ACTGGGnnnn_n'
Cuts at: 789 1634 1970 2953 3034 3369 5513 789
Size: 845 336 983 81 335 2144 1017
Fragments arranged by size:
2144 1017 983 845 336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1442 4108 1442
Size: 2666 3075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 403 775 1475 1629 1730 1898 2066 2402
Size: 188 372 700 154 101 168 168 336
Cuts at: 2402 2570 2654 215
Size: 168 84 3302
Fragments arranged by size:
3302 700 372 336 188 168 168 168
154 101 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 670 2917 3796 4288 4586 255
Size: 415 2247 879 492 298 1410
Fragments arranged by size:
2247 1410 879 492 415 298
BsaI GGTCTCn'nnnn_
Cuts at: 1036 5445 1036
Size: 4409 1332
BsaAI yAC'GTr
Cuts at: 2733 3094 2733
Size: 361 5380
BsaBI GATnn'nnATC
Cuts at: 6 3255 5292 6
Size: 3249 2037 455
Fragments arranged by size:
3249 2037 455
BsaHI Gr'CG_yC
Cuts at: 163 182 3172 3420 4122 5618 163
Size: 19 2990 248 702 1496 286
Fragments arranged by size:
2990 1496 702 286 248 19
BsaWI w'CCGG_w
Cuts at: 1792 2930 3451 4129 4403 4550 4961 1792
Size: 1138 521 678 274 147 411 2572
Fragments arranged by size:
2572 1138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 463 493 463
Size: 30 5711
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 317 484 573 317
Size: 167 89 5485
Fragments arranged by size:
5485 167 89
BseYI C'CCAG_C
Cuts at: 549 864 3166 3924 4501 4997 549
Size: 315 2302 758 577 496 1293
Fragments arranged by size:
2302 1293 758 577 496 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 2275
Size: 5741
BsiEI CG_ry'CG
Cuts at: 104 3283 3329 4537 104
Size: 3179 46 1208 1308
Fragments arranged by size:
3179 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 336 1423 2755 3533 3723 4515 336
Size: 1087 1332 778 190 792 1562
Fragments arranged by size:
1562 1332 1087 792 778 190
BsmI GAATG_Cn'
Cuts at: 2161 2335 2404 2161
Size: 174 69 5498
Fragments arranged by size:
5498 174 69
Bsp1286I G_dGCh'C
Cuts at: 329 336 646 983 1423 2755 3366 3459 3533
Size: 7 310 337 440 1332 611 93 74
Cuts at: 3533 3723 3785 4515 329
Size: 190 62 730 1555
Fragments arranged by size:
1555 1332 730 611 440 337 310 190
93 74 62 7
BspEI T'CCGG_A
Cuts at: 4961 4961
Size: 5741
BspHI T'CATG_A
Cuts at: 4917 4917
Size: 5741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2791 3005 3307 3688 2791
Size: 214 302 381 4844
Fragments arranged by size:
4844 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 307 4033 4087 5571 5736 50
Size: 118 139 3726 54 1484 165 55
Fragments arranged by size:
3726 1484 165 139 118 55 54
BsrDI GCAATG_nn'
Cuts at: 3653 3653
Size: 5741
BsrFI r'CCGG_y
Cuts at: 3739 3920 4129 3739
Size: 181 209 5351
Fragments arranged by size:
5351 209 181
BsrGI T'GTAC_A
Cuts at: 506 1035 1773 2445 506
Size: 529 738 672 3802
Fragments arranged by size:
3802 738 672 529
BssSI C'ACGA_G
Cuts at: 4012 4370 4012
Size: 358 5383
BstAPI GCAn_nnn'nTGC
Cuts at: 2156 3118 2156
Size: 962 4779
BstBI TT'CG_AA
Cuts at: 110 4102 110
Size: 3992 1749
BstYI r'GATC_y
Cuts at: 1 2557 2682 2766 3233 3256 3591 3837 4849
Size: 2556 125 84 467 23 335 246 1012
Cuts at: 4849 4935 4947 5293 1
Size: 86 12 346 449
Fragments arranged by size:
2556 1012 467 449 346 335 246 125
86 84 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 530 2350 3691 3872 4035 530
Size: 1820 1341 181 163 2236
Fragments arranged by size:
2236 1820 1341 181 163
BtsI GCAGTG_nn'
Cuts at: 350 728 2409 2984 350
Size: 378 1681 575 3107
Fragments arranged by size:
3107 1681 575 378
ClaI AT'CG_AT
Cuts at: 1649 1649
Size: 5741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 454 489 2660 2695 454
Size: 35 2171 35 3500
Fragments arranged by size:
3500 2171 35 35
DraI TTT'AAA
Cuts at: 2740 2740
Size: 5741
DrdI GACnn_nn'nnGTC
Cuts at: 3447 4305 3447
Size: 858 4883
EaeI y'GGCC_r
Cuts at: 2298 3326 3500 3891 3918 5263 2298
Size: 1028 174 391 27 1345 2776
Fragments arranged by size:
2776 1345 1028 391 174 27
EagI C'GGCC_G
Cuts at: 3326 3326
Size: 5741
EarI CTCTTCn'nnn_
Cuts at: 362 1286 3764 3974 362
Size: 924 2478 210 2129
Fragments arranged by size:
2478 2129 924 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 838 3949 4259 4405 5551 838
Size: 3111 310 146 1146 1028
Fragments arranged by size:
3111 1146 1028 310 146
EcoICRI GAG'CTC
Cuts at: 334 1421 2753 334
Size: 1087 1332 3322
Fragments arranged by size:
3322 1332 1087
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 403 775 1100 1475 1629 1650 1730 1734
Size: 188 372 325 375 154 21 80 4
Cuts at: 1734 1818 1898 1986 2066 2154 2402 2570 2574
Size: 84 80 88 80 88 248 168 4
Cuts at: 2574 2654 3566 3998 4745 5013 215
Size: 80 912 432 747 268 943
Fragments arranged by size:
943 912 747 432 375 372 325 268
248 188 168 154 88 88 84 80
80 80 80 21 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1231 1231
Size: 5741
EcoO109I rG'GnC_Cy
Cuts at: 319 2880 319
Size: 2561 3180
EcoRI G'AATT_C
Cuts at: 1445 1700 2624 2745 1445
Size: 255 924 121 4441
Fragments arranged by size:
4441 924 255 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 711 3760 711
Size: 3049 2692
FspI TGC'GCA
Cuts at: 3522 3522
Size: 5741
HaeII r_GCGC'y
Cuts at: 185 3175 3423 4445 5424 185
Size: 2990 248 1022 979 502
Fragments arranged by size:
2990 1022 979 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 565 597 904 936 940 972 1084 1116 1492
Size: 32 307 32 4 32 112 32 376
Cuts at: 1492 1524 1602 1634 2251 2283 3583 3613 3615
Size: 32 78 32 617 32 1300 30 2
Cuts at: 3615 3645 565
Size: 30 2661
Fragments arranged by size:
2661 1300 617 376 307 112 78 32
32 32 32 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 843 2645 2780 843
Size: 1802 135 3804
Fragments arranged by size:
3804 1802 135
HindIII A'AGCT_T
Cuts at: 1168 1378 1574 1913 1168
Size: 210 196 339 4996
Fragments arranged by size:
4996 339 210 196
KasI G'GCGC_C
Cuts at: 181 3171 3419 181
Size: 2990 248 2503
Fragments arranged by size:
2990 2503 248
KpnI G_GTAC'C
Cuts at: 2761 2761
Size: 5741
MluI A'CGCG_T
Cuts at: 5174 5174
Size: 5741
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 503 569 4412 4596 503
Size: 66 3843 184 1648
Fragments arranged by size:
3843 1648 184 66
MscI TGG'CCA
Cuts at: 2300 3502 2300
Size: 1202 4539
MslI CAynn'nnrTG
Cuts at: 1307 1809 2145 2213 2481 3730 3857 1307
Size: 502 336 68 268 1249 127 3191
Fragments arranged by size:
3191 1249 502 336 268 127 68
MspA1I CmG'CkG
Cuts at: 2852 3166 3526 4539 4784 2852
Size: 314 360 1013 245 3809
Fragments arranged by size:
3809 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3922 3922
Size: 5741
NarI GG'CG_CC
Cuts at: 182 3172 3420 182
Size: 2990 248 2503
Fragments arranged by size:
2990 2503 248
NdeI CA'TA_TG
Cuts at: 1074 1806 2142 2478 1074
Size: 732 336 336 4337
Fragments arranged by size:
4337 732 336 336
NgoMIV G'CCGG_C
Cuts at: 3920 3920
Size: 5741
NsiI A_TGCA'T
Cuts at: 1848 1848
Size: 5741
NspI r_CATG'y
Cuts at: 2651 2794 3294 4201 2651
Size: 143 500 907 4191
Fragments arranged by size:
4191 907 500 143
PasI CC'CwG_GG
Cuts at: 423 423
Size: 5741
PciI A'CATG_T
Cuts at: 4197 4197
Size: 5741
PflMI CCAn_nnn'nTGG
Cuts at: 429 429
Size: 5741
PmeI GTTT'AAAC
Cuts at: 2740 2740
Size: 5741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4880 4912 4880
Size: 32 5709
PsiI TTA'TAA
Cuts at: 1469 2564 1469
Size: 1095 4646
PstI C_TGCA'G
Cuts at: 2788 3473 2788
Size: 685 5056
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5741
PvuII CAG'CTG
Cuts at: 3166 3526 3166
Size: 360 5381
RsrII CG'GwC_CG
Cuts at: 3936 3936
Size: 5741
SacI G_AGCT'C
Cuts at: 336 1423 2755 336
Size: 1087 1332 3322
Fragments arranged by size:
3322 1332 1087
SalI G'TCGA_C
Cuts at: 2778 2778
Size: 5741
SapI GCTCTTCn'nnn_
Cuts at: 362 3764 3974 362
Size: 3402 210 2129
Fragments arranged by size:
3402 2129 210
SbfI CC_TGCA'GG
Cuts at: 2788 2788
Size: 5741
ScaI AGT'ACT
Cuts at: 760 5258 760
Size: 4498 1243
SfcI C'TryA_G
Cuts at: 33 221 452 531 2784 3469 4462 4653 33
Size: 188 231 79 2253 685 993 191 1121
Fragments arranged by size:
2253 1121 993 685 231 191 188 79
SfoI GGC'GCC
Cuts at: 183 3173 3421 183
Size: 2990 248 2503
Fragments arranged by size:
2990 2503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5741
SmaI CCC'GGG
Cuts at: 173 202 256 2763 173
Size: 29 54 2507 3151
Fragments arranged by size:
3151 2507 54 29
SmlI C'TyrA_G
Cuts at: 270 685 2896 3811 4303 4565 270
Size: 415 2211 915 492 262 1446
Fragments arranged by size:
2211 1446 915 492 415 262
SnaBI TAC'GTA
Cuts at: 2733 2733
Size: 5741
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5741
SphI G_CATG'C
Cuts at: 2794 3294 2794
Size: 500 5241
StuI AGG'CCT
Cuts at: 1492 2251 1492
Size: 759 4982
StyI C'CwwG_G
Cuts at: 239 607 2875 3229 239
Size: 368 2268 354 2751
Fragments arranged by size:
2751 2268 368 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 878 2104 3324 3457 3916 4125 878
Size: 1226 1220 133 459 209 2494
Fragments arranged by size:
2494 1226 1220 459 209 133
TatI w'GTAC_w
Cuts at: 506 758 1035 1773 1869 2272 2445 5256 506
Size: 252 277 738 96 403 173 2811 991
Fragments arranged by size:
2811 991 738 403 277 252 173 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 342 374 463 495 920 952 2658 2690 5258
Size: 32 89 32 425 32 1706 32 2568
Cuts at: 5258 5290 342
Size: 32 793
Fragments arranged by size:
2568 1706 793 425 89 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3538 3538
Size: 5741
XbaI T'CTAG_A
Cuts at: 2772 2772
Size: 5741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3236 3236
Size: 5741
XmaI C'CCGG_G
Cuts at: 171 200 254 2761 171
Size: 29 54 2507 3151
Fragments arranged by size:
3151 2507 54 29
XmnI GAAnn'nnTTC
Cuts at: 694 877 1055 1576 1663 2460 2503 694
Size: 183 178 521 87 797 43 3932
Fragments arranged by size:
3932 797 521 183 178 87 43
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI DraIII
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI PacI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI ZraI