Restriction map for
pF1KSDA1399
(Circular) (Six-base) MAPSORT of: pF1KSDA1399.seq Check: 8222 from: 1 to: 4367
pF1KSDA1399 4367 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 513 513
Size: 4367
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 2192 2624 3371 3639 292
Size: 1900 432 747 268 1020
Fragments arranged by size:
1900 1020 747 432 268
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AleI CACnn'nnGTG
Cuts at: 1253 1351 1253
Size: 98 4269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1223 1223
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 1672 3239 1672
Size: 1567 2800
ApaI G_GGCC'C
Cuts at: 403 1207 403
Size: 804 3563
ApaLI G'TGCA_C
Cuts at: 684 3137 684
Size: 2453 1914
ApoI r'AATT_y
Cuts at: 1371 3720 3976 1371
Size: 2349 256 1762
Fragments arranged by size:
2349 1762 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 404 1387 404
Size: 983 3384
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 290 373 1183 1383 1797 2045 2080 290
Size: 83 810 200 414 248 35 2577
Fragments arranged by size:
2577 810 414 248 200 83 35
BanII G_rGCy'C
Cuts at: 403 428 658 859 1207 1381 2411 403
Size: 25 230 201 348 174 1030 2359
Fragments arranged by size:
2359 1030 348 230 201 174 25
BbeI G_GCGC'C
Cuts at: 294 377 1801 2049 294
Size: 83 1424 248 2612
Fragments arranged by size:
2612 1424 248 83
BbsI GAAGACnn'nnnn_
Cuts at: 559 559
Size: 4367
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglII A'GATC_T
Cuts at: 947 1882 3919 947
Size: 935 2037 1395
Fragments arranged by size:
2037 1395 935
BlpI GC'TnA_GC
Cuts at: 517 652 1479 517
Size: 135 827 3405
Fragments arranged by size:
3405 827 135
Bme1580I G_kGCm'C
Cuts at: 204 403 688 1207 1992 2085 3141 204
Size: 199 285 519 785 93 1056 1430
Fragments arranged by size:
1430 1056 785 519 285 199 93
BmgBI CAC'GTC
Cuts at: 765 978 765
Size: 213 4154
BmrI ACTGGGnnnn_n'
Cuts at: 489 583 1579 1660 1995 4139 489
Size: 94 996 81 335 2144 717
Fragments arranged by size:
2144 996 717 335 94 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 753 1114 414
Size: 339 361 3667
Fragments arranged by size:
3667 361 339
Bpu10I CC'TnA_GC
Cuts at: 1196 1196
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 284 1308 1543 2422 2914 3212 223
Size: 61 1024 235 879 492 298 1378
Fragments arranged by size:
1378 1024 879 492 298 235 61
BsaI GGTCTCn'nnnn_
Cuts at: 238 997 1168 1284 4071 238
Size: 759 171 116 2787 534
Fragments arranged by size:
2787 759 534 171 116
BsaAI yAC'GTr
Cuts at: 1359 1720 1359
Size: 361 4006
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 291 374 1798 2046 2748 4244 291
Size: 83 1424 248 702 1496 414
Fragments arranged by size:
1496 1424 702 414 248 83
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 287 519 1125 1152 1186 226
Size: 61 232 606 27 34 3407
Fragments arranged by size:
3407 606 232 61 34 27
BseYI C'CCAG_C
Cuts at: 204 329 489 1792 2550 3127 3623 204
Size: 125 160 1303 758 577 496 948
Fragments arranged by size:
1303 948 758 577 496 160 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 1255
Size: 4367
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 688 1381 2159 2349 3141 688
Size: 693 778 190 792 1914
Fragments arranged by size:
1914 792 778 693 190
Bsp1286I G_dGCh'C
Cuts at: 204 403 428 658 688 859 1207 1381 1992
Size: 199 25 230 30 171 348 174 611
Cuts at: 1992 2085 2159 2349 2411 3141 204
Size: 93 74 190 62 730 1430
Fragments arranged by size:
1430 730 611 348 230 199 190 174
171 93 74 62 30 25
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 1296 1337 3543 1296
Size: 41 2206 2120
Fragments arranged by size:
2206 2120 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 513 1417 1631 1933 2314 513
Size: 904 214 302 381 2566
Fragments arranged by size:
2566 904 381 302 214
BsrBI CCG'CTC
Cuts at: 50 723 2659 2713 4197 4362 50
Size: 673 1936 54 1484 165 55
Fragments arranged by size:
1936 1484 673 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 1219 2365 2546 2755 1219
Size: 1146 181 209 2831
Fragments arranged by size:
2831 1146 209 181
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 110 2728 110
Size: 2618 1749
BstYI r'GATC_y
Cuts at: 1 947 1392 1859 1882 2217 2463 3475 3561
Size: 946 445 467 23 335 246 1012 86
Cuts at: 3561 3573 3919 1
Size: 12 346 449
Fragments arranged by size:
1012 946 467 449 445 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1230 1230
Size: 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 753 855 2317 2498 2661 753
Size: 102 1462 181 163 2459
Fragments arranged by size:
2459 1462 181 163 102
BtsI GCAGTG_nn'
Cuts at: 528 802 1063 1610 528
Size: 274 261 547 3285
Fragments arranged by size:
3285 547 274 261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 434 469 434
Size: 35 4332
DraI TTT'AAA
Cuts at: 1032 1366 1032
Size: 334 4033
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 347 1221 1952 2126 2517 2544 3889 347
Size: 874 731 174 391 27 1345 825
Fragments arranged by size:
1345 874 825 731 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 265 868 946 1163 2390 2600 265
Size: 603 78 217 1227 210 2032
Fragments arranged by size:
2032 1227 603 217 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 414 753 1114 2192 2624 3371 3639 292
Size: 122 339 361 1078 432 747 268 1020
Fragments arranged by size:
1078 1020 747 432 361 339 268 122
EcoO109I rG'GnC_Cy
Cuts at: 399 400 602 1009 1203 1204 1234 1506 399
Size: 1 202 407 194 1 30 272 3260
Fragments arranged by size:
3260 407 272 202 194 30 1 1
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 2386 297
Size: 2089 2278
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 294 307 377 930 1801 2049 3071 4050 294
Size: 13 70 553 871 248 1022 979 611
Fragments arranged by size:
1022 979 871 611 553 248 70 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 553 578 585 1319 1351 2209 2239 2241
Size: 7 25 7 734 32 858 30 2
Cuts at: 2241 2271 546
Size: 30 2642
Fragments arranged by size:
2642 858 734 32 30 30 25 7
7 2
HincII GTy'rAC
Cuts at: 899 1050 1406 899
Size: 151 356 3860
Fragments arranged by size:
3860 356 151
KasI G'GCGC_C
Cuts at: 290 373 1797 2045 290
Size: 83 1424 248 2612
Fragments arranged by size:
2612 1424 248 83
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3038 3222 3038
Size: 184 4183
MscI TGG'CCA
Cuts at: 2128 2128
Size: 4367
MslI CAynn'nnrTG
Cuts at: 130 976 1253 1351 2356 2483 130
Size: 846 277 98 1005 127 2014
Fragments arranged by size:
2014 1005 846 277 127 98
MspA1I CmG'CkG
Cuts at: 248 665 1478 1792 2152 3165 3410 248
Size: 417 813 314 360 1013 245 1205
Fragments arranged by size:
1205 1013 813 417 360 314 245
NaeI GCC'GGC
Cuts at: 1221 2548 1221
Size: 1327 3040
NarI GG'CG_CC
Cuts at: 291 374 1798 2046 291
Size: 83 1424 248 2612
Fragments arranged by size:
2612 1424 248 83
NgoMIV G'CCGG_C
Cuts at: 1219 2546 1219
Size: 1327 3040
NspI r_CATG'y
Cuts at: 258 1420 1920 2827 258
Size: 1162 500 907 1798
Fragments arranged by size:
1798 1162 907 500
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 602 1009 602
Size: 407 3960
PspOMI G'GGCC_C
Cuts at: 399 1203 399
Size: 804 3563
PstI C_TGCA'G
Cuts at: 797 1414 2099 797
Size: 617 685 3065
Fragments arranged by size:
3065 685 617
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 265 2390 2600 265
Size: 2125 210 2032
Fragments arranged by size:
2125 2032 210
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 793 1410 2095 3088 3279 33
Size: 760 617 685 993 191 1121
Fragments arranged by size:
1121 993 760 685 617 191
SfoI GGC'GCC
Cuts at: 292 375 1799 2047 292
Size: 83 1424 248 2612
Fragments arranged by size:
2612 1424 248 83
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 238 299 1287 1522 2437 2929 3191 238
Size: 61 988 235 915 492 262 1414
Fragments arranged by size:
1414 988 915 492 262 235 61
SnaBI TAC'GTA
Cuts at: 1359 1359
Size: 4367
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 258 1420 1920 258
Size: 1162 500 2705
Fragments arranged by size:
2705 1162 500
StyI C'CwwG_G
Cuts at: 1501 1855 1501
Size: 354 4013
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 666 1159 1950 2083 2542 2751 571
Size: 95 493 791 133 459 209 2187
Fragments arranged by size:
2187 791 493 459 209 133 95
TatI w'GTAC_w
Cuts at: 3882 3882
Size: 4367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 771 803 967 999 1001 1033 3884 3916 771
Size: 32 164 32 2 32 2851 32 1222
Fragments arranged by size:
2851 1222 164 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
XmnI GAAnn'nnTTC
Cuts at: 277 277
Size: 4367
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI ZraI