Restriction map for
pF1KSDA1383
(Circular) (Six-base) MAPSORT of: pF1KSDA1383.seq Check: 5003 from: 1 to: 5852
pF1KSDA1383 5852 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2890 2890
Size: 5852
Acc65I G'GTAC_C
Cuts at: 2868 2868
Size: 5852
AclI AA'CG_TT
Cuts at: 5692 5692
Size: 5852
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1498 1820 2239 2276 3677 4109 4856 5124
Size: 17 322 419 37 1401 432 747 268
Cuts at: 5124 1481
Size: 2209
Fragments arranged by size:
2209 1401 747 432 419 322 268 37
17
AflIII A'CryG_T
Cuts at: 940 1267 3204 4308 5285 940
Size: 327 1937 1104 977 1507
Fragments arranged by size:
1937 1507 1104 977 327
AgeI A'CCGG_T
Cuts at: 4240 4240
Size: 5852
AleI CACnn'nnGTG
Cuts at: 2368 2368
Size: 5852
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 5820
AlwNI CAG_nnn'CTG
Cuts at: 519 777 952 3157 4724 519
Size: 258 175 2205 1567 1647
Fragments arranged by size:
2205 1647 1567 258 175
ApaLI G'TGCA_C
Cuts at: 4622 4622
Size: 5852
ApoI r'AATT_y
Cuts at: 1729 1736 1992 2455 2856 5205 5461 1729
Size: 7 256 463 401 2349 256 2120
Fragments arranged by size:
2349 2120 463 401 256 256 7
AseI AT'TA_AT
Cuts at: 20 1292 20
Size: 1272 4580
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
AvaI C'yCGr_G
Cuts at: 2872 2872
Size: 5852
BamHI G'GATC_C
Cuts at: 2877 2877
Size: 5852
BanI G'GyrC_C
Cuts at: 494 1576 2868 3282 3530 3565 494
Size: 1082 1292 414 248 35 2781
Fragments arranged by size:
2781 1292 1082 414 248 35
BanII G_rGCy'C
Cuts at: 1883 2604 2866 3896 1883
Size: 721 262 1030 3839
Fragments arranged by size:
3839 1030 721 262
BbeI G_GCGC'C
Cuts at: 498 3286 3534 498
Size: 2788 248 2816
Fragments arranged by size:
2816 2788 248
BbsI GAAGACnn'nnnn_
Cuts at: 1504 1504
Size: 5852
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1420 1454 1420
Size: 34 5818
BciVI GTATCCnnnnn_n'
Cuts at: 2824 3750 4511 5852 2824
Size: 926 761 1341 2824
Fragments arranged by size:
2824 1341 926 761
BclI T'GATC_A
Cuts at: 3372 3372
Size: 5852
BfrBI ATG'CAT
Cuts at: 1825 1825
Size: 5852
BglI GCCn_nnn'nGGC
Cuts at: 492 492
Size: 5852
BglII A'GATC_T
Cuts at: 3367 5404 3367
Size: 2037 3815
BlpI GC'TnA_GC
Cuts at: 691 2964 691
Size: 2273 3579
Bme1580I G_kGCm'C
Cuts at: 289 1581 3477 3570 4626 289
Size: 1292 1896 93 1056 1515
Fragments arranged by size:
1896 1515 1292 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 186 650 3064 3145 3480 5624 186
Size: 464 2414 81 335 2144 414
Fragments arranged by size:
2414 2144 464 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4219 4219
Size: 5852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 5852
Bpu10I CC'TnA_GC
Cuts at: 2410 2410
Size: 5852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1836 2588 3028 3907 4399 4697 721
Size: 1115 752 440 879 492 298 1876
Fragments arranged by size:
1876 1115 879 752 492 440 298
BsaI GGTCTCn'nnnn_
Cuts at: 1003 5556 1003
Size: 4553 1299
BsaAI yAC'GTr
Cuts at: 2844 3205 2844
Size: 361 5491
BsaBI GATnn'nnATC
Cuts at: 6 3366 5403 6
Size: 3360 2037 455
Fragments arranged by size:
3360 2037 455
BsaHI Gr'CG_yC
Cuts at: 495 502 3283 3531 4233 5729 495
Size: 7 2781 248 702 1496 618
Fragments arranged by size:
2781 1496 702 618 248 7
BsaWI w'CCGG_w
Cuts at: 580 3041 3562 4240 4514 4661 5072 580
Size: 2461 521 678 274 147 411 1360
Fragments arranged by size:
2461 1360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 253 283 728 758 2027 2057 253
Size: 30 445 30 1269 30 4048
Fragments arranged by size:
4048 1269 445 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 256 746 749 1019 247
Size: 9 490 3 270 5080
Fragments arranged by size:
5080 490 270 9 3
BseYI C'CCAG_C
Cuts at: 758 825 1161 1882 3277 4035 4612 5108 758
Size: 67 336 721 1395 758 577 496 1502
Fragments arranged by size:
1502 1395 758 721 577 496 336 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1058 421
Size: 637 5215
BsiEI CG_ry'CG
Cuts at: 104 396 575 3394 3440 4648 104
Size: 292 179 2819 46 1208 1308
Fragments arranged by size:
2819 1308 1208 292 179 46
BsiHKAI G_wGCw'C
Cuts at: 2604 2866 3644 3834 4626 2604
Size: 262 778 190 792 3830
Fragments arranged by size:
3830 792 778 262 190
BsmI GAATG_Cn'
Cuts at: 1113 1113
Size: 5852
Bsp1286I G_dGCh'C
Cuts at: 289 1581 1883 2604 2866 3477 3570 3644 3834
Size: 1292 302 721 262 611 93 74 190
Cuts at: 3834 3896 4626 289
Size: 62 730 1515
Fragments arranged by size:
1515 1292 730 721 611 302 262 190
93 74 62
BspEI T'CCGG_A
Cuts at: 580 5072 580
Size: 4492 1360
BspHI T'CATG_A
Cuts at: 2301 5028 2301
Size: 2727 3125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2696 2902 3116 3418 3799 2696
Size: 206 214 302 381 4749
Fragments arranged by size:
4749 381 302 214 206
BsrBI CCG'CTC
Cuts at: 50 147 636 705 739 4144 4198 5682 5847
Size: 97 489 69 34 3405 54 1484 165
Cuts at: 5847 50
Size: 55
Fragments arranged by size:
3405 1484 489 165 97 69 55 54
34
BsrDI GCAATG_nn'
Cuts at: 647 963 2760 2794 3764 647
Size: 316 1797 34 970 2735
Fragments arranged by size:
2735 1797 970 316 34
BsrFI r'CCGG_y
Cuts at: 443 570 1454 3850 4031 4240 443
Size: 127 884 2396 181 209 2055
Fragments arranged by size:
2396 2055 884 209 181 127
BsrGI T'GTAC_A
Cuts at: 2439 2439
Size: 5852
BssSI C'ACGA_G
Cuts at: 4123 4481 4123
Size: 358 5494
BstAPI GCAn_nnn'nTGC
Cuts at: 407 3229 407
Size: 2822 3030
BstBI TT'CG_AA
Cuts at: 110 4213 110
Size: 4103 1749
BstEII G'GTnAC_C
Cuts at: 2432 2432
Size: 5852
BstXI CCAn_nnnn'nTGG
Cuts at: 327 327
Size: 5852
BstYI r'GATC_y
Cuts at: 1 2877 3344 3367 3702 3948 4960 5046 5058
Size: 2876 467 23 335 246 1012 86 12
Cuts at: 5058 5404 1
Size: 346 449
Fragments arranged by size:
2876 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1115 1115
Size: 5852
BtgI C'CryG_G
Cuts at: 125 293 370 125
Size: 168 77 5607
Fragments arranged by size:
5607 168 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3802 3983 4146 3802
Size: 181 163 5508
Fragments arranged by size:
5508 181 163
BtsI GCAGTG_nn'
Cuts at: 227 438 1578 3095 227
Size: 211 1140 1517 2984
Fragments arranged by size:
2984 1517 1140 211
DraI TTT'AAA
Cuts at: 1646 2851 1646
Size: 1205 4647
DrdI GACnn_nn'nnGTC
Cuts at: 3558 4416 3558
Size: 858 4994
EaeI y'GGCC_r
Cuts at: 542 572 3437 3611 4002 4029 5374 542
Size: 30 2865 174 391 27 1345 1020
Fragments arranged by size:
2865 1345 1020 391 174 30 27
EagI C'GGCC_G
Cuts at: 572 3437 572
Size: 2865 2987
EarI CTCTTCn'nnn_
Cuts at: 157 233 418 3875 4085 157
Size: 76 185 3457 210 1924
Fragments arranged by size:
3457 1924 210 185 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 406 4060 4370 4516 5662 292
Size: 114 3654 310 146 1146 482
Fragments arranged by size:
3654 1146 482 310 146 114
EcoICRI GAG'CTC
Cuts at: 2602 2864 2602
Size: 262 5590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 1481 1498 1820 2239 2276 3677 4109 4856
Size: 1300 17 322 419 37 1401 432 747
Cuts at: 4856 5124 181
Size: 268 909
Fragments arranged by size:
1401 1300 909 747 432 419 322 268
37 17
EcoNI CCTnn'n_nnAGG
Cuts at: 982 982
Size: 5852
EcoO109I rG'GnC_Cy
Cuts at: 524 2991 524
Size: 2467 3385
EcoRI G'AATT_C
Cuts at: 1736 2856 1736
Size: 1120 4732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3871 3871
Size: 5852
FseI GG_CCGG'CC
Cuts at: 574 574
Size: 5852
FspI TGC'GCA
Cuts at: 3633 3633
Size: 5852
HaeII r_GCGC'y
Cuts at: 356 498 3286 3534 4556 5535 356
Size: 142 2788 248 1022 979 673
Fragments arranged by size:
2788 1022 979 673 248 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 728 760 1408 1440 2221 2253 2310
Size: 32 443 32 648 32 781 32 57
Cuts at: 2310 2342 2482 2514 3694 3724 3726 3756 253
Size: 32 140 32 1180 30 2 30 2349
Fragments arranged by size:
2349 1180 781 648 443 140 57 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2094 2891 2094
Size: 797 5055
KasI G'GCGC_C
Cuts at: 494 3282 3530 494
Size: 2788 248 2816
Fragments arranged by size:
2816 2788 248
KpnI G_GTAC'C
Cuts at: 2872 2872
Size: 5852
MluI A'CGCG_T
Cuts at: 5285 5285
Size: 5852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2100 2614 4523 4707 2100
Size: 514 1909 184 3245
Fragments arranged by size:
3245 1909 514 184
MscI TGG'CCA
Cuts at: 544 3613 544
Size: 3069 2783
MslI CAynn'nnrTG
Cuts at: 2155 2368 3841 3968 2155
Size: 213 1473 127 4039
Fragments arranged by size:
4039 1473 213 127
MspA1I CmG'CkG
Cuts at: 214 483 777 825 2963 3277 3637 4650 4895
Size: 269 294 48 2138 314 360 1013 245
Cuts at: 4895 214
Size: 1171
Fragments arranged by size:
2138 1171 1013 360 314 294 269 245
48
NaeI GCC'GGC
Cuts at: 445 572 4033 445
Size: 127 3461 2264
Fragments arranged by size:
3461 2264 127
NarI GG'CG_CC
Cuts at: 495 3283 3531 495
Size: 2788 248 2816
Fragments arranged by size:
2816 2788 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5852
NgoMIV G'CCGG_C
Cuts at: 443 570 4031 443
Size: 127 3461 2264
Fragments arranged by size:
3461 2264 127
NsiI A_TGCA'T
Cuts at: 1827 1827
Size: 5852
NspI r_CATG'y
Cuts at: 944 1271 1454 2905 3405 4312 944
Size: 327 183 1451 500 907 2484
Fragments arranged by size:
2484 1451 907 500 327 183
PasI CC'CwG_GG
Cuts at: 490 490
Size: 5852
PciI A'CATG_T
Cuts at: 940 1267 4308 940
Size: 327 3041 2484
Fragments arranged by size:
3041 2484 327
PfoI T'CCnGG_A
Cuts at: 2717 2826 2717
Size: 109 5743
PmeI GTTT'AAAC
Cuts at: 2851 2851
Size: 5852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 4991
Size: 32 5820
PstI C_TGCA'G
Cuts at: 425 482 782 1200 1773 2899 3584 425
Size: 57 300 418 573 1126 685 2693
Fragments arranged by size:
2693 1126 685 573 418 300 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5852
PvuII CAG'CTG
Cuts at: 483 777 3277 3637 483
Size: 294 2500 360 2698
Fragments arranged by size:
2698 2500 360 294
RsrII CG'GwC_CG
Cuts at: 4047 4047
Size: 5852
SacI G_AGCT'C
Cuts at: 2604 2866 2604
Size: 262 5590
SalI G'TCGA_C
Cuts at: 2889 2889
Size: 5852
SapI GCTCTTCn'nnn_
Cuts at: 157 3875 4085 157
Size: 3718 210 1924
Fragments arranged by size:
3718 1924 210
SbfI CC_TGCA'GG
Cuts at: 2899 2899
Size: 5852
ScaI AGT'ACT
Cuts at: 5369 5369
Size: 5852
SfcI C'TryA_G
Cuts at: 33 421 478 778 1196 1769 2895 3580 4573
Size: 388 57 300 418 573 1126 685 993
Cuts at: 4573 4764 33
Size: 191 1121
Fragments arranged by size:
1126 1121 993 685 573 418 388 300
191 57
SfoI GGC'GCC
Cuts at: 496 3284 3532 496
Size: 2788 248 2816
Fragments arranged by size:
2816 2788 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
SmaI CCC'GGG
Cuts at: 2874 2874
Size: 5852
SmlI C'TyrA_G
Cuts at: 700 1851 2603 3007 3922 4414 4676 700
Size: 1151 752 404 915 492 262 1876
Fragments arranged by size:
1876 1151 915 752 492 404 262
SnaBI TAC'GTA
Cuts at: 2844 2844
Size: 5852
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5852
SphI G_CATG'C
Cuts at: 2905 3405 2905
Size: 500 5352
SspI AAT'ATT
Cuts at: 1558 2028 2778 1558
Size: 470 750 4632
Fragments arranged by size:
4632 750 470
StuI AGG'CCT
Cuts at: 265 265
Size: 5852
StyI C'CwwG_G
Cuts at: 125 2986 3340 125
Size: 2861 354 2637
Fragments arranged by size:
2861 2637 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 168 382 3435 3568 4027 4236 168
Size: 214 3053 133 459 209 1784
Fragments arranged by size:
3053 1784 459 214 209 133
TatI w'GTAC_w
Cuts at: 2439 2728 5367 2439
Size: 289 2639 2924
Fragments arranged by size:
2924 2639 289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 5369 5401 2027
Size: 32 3310 32 2478
Fragments arranged by size:
3310 2478 32 32
Tth111I GACn'n_nGTC
Cuts at: 3649 3649
Size: 5852
XbaI T'CTAG_A
Cuts at: 2883 2883
Size: 5852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3347 3347
Size: 5852
XmaI C'CCGG_G
Cuts at: 2872 2872
Size: 5852
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BmgBI BmtI BsiWI BsmBI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XhoI XmnI ZraI