Restriction map for
pF1KSDA1382
(Circular) (Six-base) MAPSORT of: pF1KSDA1382.seq Check: 9944 from: 1 to: 4655
pF1KSDA1382 4655 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1693 1693
Size: 4655
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2912 3659 3927 2480
Size: 432 747 268 3208
Fragments arranged by size:
3208 747 432 268
AflIII A'CryG_T
Cuts at: 1182 2007 3111 4088 1182
Size: 825 1104 977 1749
Fragments arranged by size:
1749 1104 977 825
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AlwNI CAG_nnn'CTG
Cuts at: 577 1960 3527 577
Size: 1383 1567 1705
Fragments arranged by size:
1705 1567 1383
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 291 319 765 844 1659 4008 4264 291
Size: 28 446 79 815 2349 256 682
Fragments arranged by size:
2349 815 682 446 256 79 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 1671 2085 2333 2368 1671
Size: 414 248 35 3958
Fragments arranged by size:
3958 414 248 35
BanII G_rGCy'C
Cuts at: 1669 2699 1669
Size: 1030 3625
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BbsI GAAGACnn'nnnn_
Cuts at: 176 502 176
Size: 326 4329
BciVI GTATCCnnnnn_n'
Cuts at: 1156 2553 3314 4655 1156
Size: 1397 761 1341 1156
Fragments arranged by size:
1397 1341 1156 761
BclI T'GATC_A
Cuts at: 2175 2175
Size: 4655
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 1767 1767
Size: 4655
Bme1580I G_kGCm'C
Cuts at: 2280 2373 3429 2280
Size: 93 1056 3506
Fragments arranged by size:
3506 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1867 1948 2283 4427 1867
Size: 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1652 1652
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4359 4359
Size: 4655
BsaAI yAC'GTr
Cuts at: 1647 2008 1647
Size: 361 4294
BsaBI GATnn'nnATC
Cuts at: 6 257 2169 4206 6
Size: 251 1912 2037 455
Fragments arranged by size:
2037 1912 455 251
BsaHI Gr'CG_yC
Cuts at: 1359 2086 2334 3036 4532 1359
Size: 727 248 702 1496 1482
Fragments arranged by size:
1496 1482 727 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1585 1844 2365 3043 3317 3464 3875 1307
Size: 278 259 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 278 274 259 147
BseYI C'CCAG_C
Cuts at: 2080 2838 3415 3911 2080
Size: 758 577 496 2824
Fragments arranged by size:
2824 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1669 2447 2637 3429 1669
Size: 778 190 792 2895
Fragments arranged by size:
2895 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1669 2280 2373 2447 2637 2699 3429 1669
Size: 611 93 74 190 62 730 2895
Fragments arranged by size:
2895 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1307 3875 1307
Size: 2568 2087
BspHI T'CATG_A
Cuts at: 3831 3831
Size: 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 939 1705 1919 2221 2602 939
Size: 766 214 302 381 2992
Fragments arranged by size:
2992 766 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2947 3001 4485 4650 50
Size: 2897 54 1484 165 55
Fragments arranged by size:
2897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 375 2567 375
Size: 2192 2463
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BsrGI T'GTAC_A
Cuts at: 1533 1533
Size: 4655
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 2032 2032
Size: 4655
BstBI TT'CG_AA
Cuts at: 110 3016 110
Size: 2906 1749
BstYI r'GATC_y
Cuts at: 1 258 1680 2147 2170 2505 2751 3763 3849
Size: 257 1422 467 23 335 246 1012 86
Cuts at: 3849 3861 4207 1
Size: 12 346 449
Fragments arranged by size:
1422 1012 467 449 346 335 257 246
86 23 12
BtgI C'CryG_G
Cuts at: 410 410
Size: 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 390 1898 390
Size: 1508 3147
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 456 2361 3219 456
Size: 1905 858 1892
Fragments arranged by size:
1905 1892 858
EaeI y'GGCC_r
Cuts at: 1638 2240 2414 2805 2832 4177 1638
Size: 602 174 391 27 1345 2116
Fragments arranged by size:
2116 1345 602 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 628 1218 2678 2888 628
Size: 590 1460 210 2395
Fragments arranged by size:
2395 1460 590 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1652 2480 2912 3659 3927 1652
Size: 828 432 747 268 2380
Fragments arranged by size:
2380 828 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1794 1794
Size: 4655
EcoRI G'AATT_C
Cuts at: 1659 1659
Size: 4655
EcoRV GAT'ATC
Cuts at: 1434 1434
Size: 4655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4655
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 2089 2337 3359 4338 2089
Size: 248 1022 979 2406
Fragments arranged by size:
2406 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2497 2527 2529 2559 2497
Size: 30 2 30 4593
Fragments arranged by size:
4593 30 30 2
HincII GTy'rAC
Cuts at: 1694 1694
Size: 4655
HindIII A'AGCT_T
Cuts at: 565 565
Size: 4655
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MfeI C'AATT_G
Cuts at: 532 532
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 215 540 3326 3510 215
Size: 325 2786 184 1360
Fragments arranged by size:
2786 1360 325 184
MscI TGG'CCA
Cuts at: 1640 2416 1640
Size: 776 3879
MslI CAynn'nnrTG
Cuts at: 1375 2644 2771 1375
Size: 1269 127 3259
Fragments arranged by size:
3259 1269 127
MspA1I CmG'CkG
Cuts at: 1766 2080 2440 3453 3698 1766
Size: 314 360 1013 245 2723
Fragments arranged by size:
2723 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NcoI C'CATG_G
Cuts at: 410 410
Size: 4655
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NspI r_CATG'y
Cuts at: 1186 1708 2208 3115 1186
Size: 522 500 907 2726
Fragments arranged by size:
2726 907 522 500
PciI A'CATG_T
Cuts at: 1182 3111 1182
Size: 1929 2726
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4623
PsiI TTA'TAA
Cuts at: 439 439
Size: 4655
PstI C_TGCA'G
Cuts at: 1461 1702 2387 1461
Size: 241 685 3729
Fragments arranged by size:
3729 685 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 2080 2440 2080
Size: 360 4295
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 4172 4172
Size: 4655
SfcI C'TryA_G
Cuts at: 33 183 1457 1698 2383 3376 3567 33
Size: 150 1274 241 685 993 191 1121
Fragments arranged by size:
1274 1121 993 685 241 191 150
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 1810 2725 3217 3479 1810
Size: 915 492 262 2986
Fragments arranged by size:
2986 915 492 262
SnaBI TAC'GTA
Cuts at: 1647 1647
Size: 4655
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
SspI AAT'ATT
Cuts at: 464 464
Size: 4655
StyI C'CwwG_G
Cuts at: 393 410 1789 2143 393
Size: 17 1379 354 2905
Fragments arranged by size:
2905 1379 354 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2238 2371 2830 3039 2238
Size: 133 459 209 3854
Fragments arranged by size:
3854 459 209 133
TatI w'GTAC_w
Cuts at: 1533 1572 4170 1533
Size: 39 2598 2018
Fragments arranged by size:
2598 2018 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 4623
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 4655
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
XmnI GAAnn'nnTTC
Cuts at: 1199 1199
Size: 4655
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI