Restriction map for
pF1KSDA1363
(Circular) (Six-base) MAPSORT of: pF1KSDA1363.seq Check: 9204 from: 1 to: 4361
pF1KSDA1363 4361 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 308 308
Size: 4361
AccI GT'mk_AC
Cuts at: 182 1399 182
Size: 1217 3144
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 2186 2618 3365 3633 655
Size: 1531 432 747 268 1383
Fragments arranged by size:
1531 1383 747 432 268
AflIII A'CryG_T
Cuts at: 1156 1713 2817 3794 1156
Size: 557 1104 977 1723
Fragments arranged by size:
1723 1104 977 557
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 1083 1666 3233 1083
Size: 583 1567 2211
Fragments arranged by size:
2211 1567 583
ApaI G_GGCC'C
Cuts at: 978 978
Size: 4361
ApaLI G'TGCA_C
Cuts at: 251 3131 251
Size: 2880 1481
ApoI r'AATT_y
Cuts at: 312 599 677 1365 3714 3970 312
Size: 287 78 688 2349 256 703
Fragments arranged by size:
2349 703 688 287 256 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 1381 1381
Size: 4361
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 1377 1791 2039 2074 1377
Size: 414 248 35 3664
Fragments arranged by size:
3664 414 248 35
BanII G_rGCy'C
Cuts at: 978 1375 2405 978
Size: 397 1030 2934
Fragments arranged by size:
2934 1030 397
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BbsI GAAGACnn'nnnn_
Cuts at: 344 344
Size: 4361
BbvCI CC'TCA_GC
Cuts at: 530 530
Size: 4361
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 1881 1881
Size: 4361
BglI GCCn_nnn'nGGC
Cuts at: 160 160
Size: 4361
BglII A'GATC_T
Cuts at: 1876 3913 1876
Size: 2037 2324
BlpI GC'TnA_GC
Cuts at: 1473 1473
Size: 4361
Bme1580I G_kGCm'C
Cuts at: 255 978 1986 2079 3135 255
Size: 723 1008 93 1056 1481
Fragments arranged by size:
1481 1056 1008 723 93
BmgBI CAC'GTC
Cuts at: 1157 1157
Size: 4361
BmrI ACTGGGnnnn_n'
Cuts at: 1573 1654 1989 4133 1573
Size: 81 335 2144 1801
Fragments arranged by size:
2144 1801 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4361
Bpu10I CC'TnA_GC
Cuts at: 530 593 530
Size: 63 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1537 2416 2908 3206 725
Size: 812 879 492 298 1880
Fragments arranged by size:
1880 879 812 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4065 4065
Size: 4361
BsaAI yAC'GTr
Cuts at: 1353 1714 1353
Size: 361 4000
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 236 1792 2040 2742 4238 236
Size: 1556 248 702 1496 359
Fragments arranged by size:
1556 1496 702 359 248
BsaWI w'CCGG_w
Cuts at: 1307 1550 2071 2749 3023 3170 3581 1307
Size: 243 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 243 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 482 1123 482
Size: 641 3720
BseYI C'CCAG_C
Cuts at: 473 1786 2544 3121 3617 473
Size: 1313 758 577 496 1217
Fragments arranged by size:
1313 1217 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 934
Size: 4361
BsiEI CG_ry'CG
Cuts at: 104 1903 1949 3157 104
Size: 1799 46 1208 1308
Fragments arranged by size:
1799 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 255 1375 2153 2343 3135 255
Size: 1120 778 190 792 1481
Fragments arranged by size:
1481 1120 792 778 190
BsmI GAATG_Cn'
Cuts at: 546 546
Size: 4361
BsmBI CGTCTCn'nnnn_
Cuts at: 1172 1172
Size: 4361
Bsp1286I G_dGCh'C
Cuts at: 255 978 1375 1986 2079 2153 2343 2405 3135
Size: 723 397 611 93 74 190 62 730
Cuts at: 3135 255
Size: 1481
Fragments arranged by size:
1481 730 723 611 397 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1307 3581 1307
Size: 2274 2087
BspHI T'CATG_A
Cuts at: 603 3537 603
Size: 2934 1427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 308 1411 1625 1927 2308 308
Size: 1103 214 302 381 2361
Fragments arranged by size:
2361 1103 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1094 2653 2707 4191 4356 50
Size: 1044 1559 54 1484 165 55
Fragments arranged by size:
1559 1484 1044 165 55 54
BsrDI GCAATG_nn'
Cuts at: 530 926 1056 1106 2273 530
Size: 396 130 50 1167 2618
Fragments arranged by size:
2618 1167 396 130 50
BsrFI r'CCGG_y
Cuts at: 1216 2359 2540 2749 1216
Size: 1143 181 209 2828
Fragments arranged by size:
2828 1143 209 181
BsrGI T'GTAC_A
Cuts at: 517 1035 517
Size: 518 3843
BssSI C'ACGA_G
Cuts at: 2632 2990 2632
Size: 358 4003
BstAPI GCAn_nnn'nTGC
Cuts at: 680 1738 680
Size: 1058 3303
BstBI TT'CG_AA
Cuts at: 110 2722 110
Size: 2612 1749
BstEII G'GTnAC_C
Cuts at: 369 1227 369
Size: 858 3503
BstXI CCAn_nnnn'nTGG
Cuts at: 1206 1206
Size: 4361
BstYI r'GATC_y
Cuts at: 1 1386 1853 1876 2211 2457 3469 3555 3567
Size: 1385 467 23 335 246 1012 86 12
Cuts at: 3567 3913 1
Size: 346 449
Fragments arranged by size:
1385 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 462 462
Size: 4361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2311 2492 2655 2311
Size: 181 163 4017
Fragments arranged by size:
4017 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1604 1127
Size: 477 3884
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 167 1946 2120 2511 2538 3883 167
Size: 1779 174 391 27 1345 645
Fragments arranged by size:
1779 1345 645 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 425 950 2384 2594 425
Size: 525 1434 210 2192
Fragments arranged by size:
2192 1434 525 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1086 2569 2879 3025 4171 1086
Size: 1483 310 146 1146 1276
Fragments arranged by size:
1483 1276 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 2186 2618 3365 3633 655
Size: 1531 432 747 268 1383
Fragments arranged by size:
1531 1383 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 415 974 1500 415
Size: 559 526 3276
Fragments arranged by size:
3276 559 526
EcoRI G'AATT_C
Cuts at: 1365 1365
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 166 1795 2043 3065 4044 166
Size: 1629 248 1022 979 483
Fragments arranged by size:
1629 1022 979 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1078 1110 1221 1253 2203 2233 2235 2265 1078
Size: 32 111 32 950 30 2 30 3174
Fragments arranged by size:
3174 950 111 32 32 30 30 2
HincII GTy'rAC
Cuts at: 930 1400 930
Size: 470 3891
HindIII A'AGCT_T
Cuts at: 776 800 776
Size: 24 4337
HpaI GTT'AAC
Cuts at: 930 930
Size: 4361
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 3032 3216 233
Size: 2799 184 1378
Fragments arranged by size:
2799 1378 184
MscI TGG'CCA
Cuts at: 169 2122 169
Size: 1953 2408
MslI CAynn'nnrTG
Cuts at: 2350 2477 2350
Size: 127 4234
MspA1I CmG'CkG
Cuts at: 192 1472 1786 2146 3159 3404 192
Size: 1280 314 360 1013 245 1149
Fragments arranged by size:
1280 1149 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2542 2542
Size: 4361
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NgoMIV G'CCGG_C
Cuts at: 2540 2540
Size: 4361
NspI r_CATG'y
Cuts at: 1414 1914 2821 1414
Size: 500 907 2954
Fragments arranged by size:
2954 907 500
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PflMI CCAn_nnn'nTGG
Cuts at: 282 282
Size: 4361
PfoI T'CCnGG_A
Cuts at: 1064 1064
Size: 4361
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 4329
PspOMI G'GGCC_C
Cuts at: 974 974
Size: 4361
PstI C_TGCA'G
Cuts at: 1130 1408 2093 1130
Size: 278 685 3398
Fragments arranged by size:
3398 685 278
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 1786 2146 1786
Size: 360 4001
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 425 2384 2594 425
Size: 1959 210 2192
Fragments arranged by size:
2192 1959 210
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 3878 3878
Size: 4361
SfcI C'TryA_G
Cuts at: 33 1126 1404 2089 3082 3273 33
Size: 1093 278 685 993 191 1121
Fragments arranged by size:
1121 1093 993 685 278 191
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 740 1516 2431 2923 3185 740
Size: 776 915 492 262 1916
Fragments arranged by size:
1916 915 776 492 262
SnaBI TAC'GTA
Cuts at: 1353 1353
Size: 4361
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StyI C'CwwG_G
Cuts at: 479 723 1495 1849 479
Size: 244 772 354 2991
Fragments arranged by size:
2991 772 354 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 388 870 1944 2077 2536 2745 388
Size: 482 1074 133 459 209 2004
Fragments arranged by size:
2004 1074 482 459 209 133
TatI w'GTAC_w
Cuts at: 517 1035 3876 517
Size: 518 2841 1002
Fragments arranged by size:
2841 1002 518
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4329
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1296 1856 1296
Size: 560 3801
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BpmI BsaXI BsiWI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI ZraI