Restriction map for
pF1KSDA1344
(Circular) (Six-base) MAPSORT of: pF1KSDA1344.seq Check: 8981 from: 1 to: 5612
pF1KSDA1344 5612 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2650 2650
Size: 5612
Acc65I G'GTAC_C
Cuts at: 2628 2628
Size: 5612
AclI AA'CG_TT
Cuts at: 5452 5452
Size: 5612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 905 914 1829 1993 2228 2305 3437 3869
Size: 355 9 915 164 235 77 1132 432
Cuts at: 3869 4616 4884 550
Size: 747 268 1278
Fragments arranged by size:
1278 1132 915 747 432 355 268 235
164 77 9
AflIII A'CryG_T
Cuts at: 2964 4068 5045 2964
Size: 1104 977 3531
Fragments arranged by size:
3531 1104 977
AgeI A'CCGG_T
Cuts at: 4000 4000
Size: 5612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 469 469
Size: 5612
AlwNI CAG_nnn'CTG
Cuts at: 1261 2917 4484 1261
Size: 1656 1567 2389
Fragments arranged by size:
2389 1656 1567
ApaLI G'TGCA_C
Cuts at: 4382 4382
Size: 5612
ApoI r'AATT_y
Cuts at: 353 463 1123 1513 1594 1799 2616 4965 5221
Size: 110 660 390 81 205 817 2349 256
Cuts at: 5221 353
Size: 744
Fragments arranged by size:
2349 817 744 660 390 256 205 110
81
AseI AT'TA_AT
Cuts at: 20 20
Size: 5612
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
AvaI C'yCGr_G
Cuts at: 2632 2632
Size: 5612
BamHI G'GATC_C
Cuts at: 2637 2637
Size: 5612
BanI G'GyrC_C
Cuts at: 2628 3042 3290 3325 2628
Size: 414 248 35 4915
Fragments arranged by size:
4915 414 248 35
BanII G_rGCy'C
Cuts at: 2626 3656 2626
Size: 1030 4582
BbeI G_GCGC'C
Cuts at: 3046 3294 3046
Size: 248 5364
BbsI GAAGACnn'nnnn_
Cuts at: 566 1006 1083 1211 1843 566
Size: 440 77 128 632 4335
Fragments arranged by size:
4335 632 440 128 77
BciVI GTATCCnnnnn_n'
Cuts at: 1398 3510 4271 5612 1398
Size: 2112 761 1341 1398
Fragments arranged by size:
2112 1398 1341 761
BclI T'GATC_A
Cuts at: 3132 3132
Size: 5612
BglII A'GATC_T
Cuts at: 1584 3127 5164 1584
Size: 1543 2037 2032
Fragments arranged by size:
2037 2032 1543
BlpI GC'TnA_GC
Cuts at: 2724 2724
Size: 5612
Bme1580I G_kGCm'C
Cuts at: 3237 3330 4386 3237
Size: 93 1056 4463
Fragments arranged by size:
4463 1056 93
BmgBI CAC'GTC
Cuts at: 2569 2569
Size: 5612
BmrI ACTGGGnnnn_n'
Cuts at: 940 1546 1988 2824 2905 3240 5384 940
Size: 606 442 836 81 335 2144 1168
Fragments arranged by size:
2144 1168 836 606 442 335 81
BmtI G_CTAG'C
Cuts at: 1913 1913
Size: 5612
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1054 3979 1054
Size: 2925 2687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2123
Size: 5612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 2318 2788 3667 4159 4457 1058
Size: 1260 470 879 492 298 2213
Fragments arranged by size:
2213 1260 879 492 470 298
BsaI GGTCTCn'nnnn_
Cuts at: 5316 5316
Size: 5612
BsaAI yAC'GTr
Cuts at: 2604 2965 2604
Size: 361 5251
BsaBI GATnn'nnATC
Cuts at: 6 3126 5163 6
Size: 3120 2037 455
Fragments arranged by size:
3120 2037 455
BsaHI Gr'CG_yC
Cuts at: 1011 3043 3291 3993 5489 1011
Size: 2032 248 702 1496 1134
Fragments arranged by size:
2032 1496 1134 702 248
BsaWI w'CCGG_w
Cuts at: 1968 2801 3322 4000 4274 4421 4832 1968
Size: 833 521 678 274 147 411 2748
Fragments arranged by size:
2748 833 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1103 1133 1103
Size: 30 5582
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2339 2339
Size: 5612
BseYI C'CCAG_C
Cuts at: 794 3037 3795 4372 4868 794
Size: 2243 758 577 496 1538
Fragments arranged by size:
2243 1538 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1493 2464 1493
Size: 971 4641
BsiEI CG_ry'CG
Cuts at: 104 3154 3200 4408 104
Size: 3050 46 1208 1308
Fragments arranged by size:
3050 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 644 2626 3404 3594 4386 644
Size: 1982 778 190 792 1870
Fragments arranged by size:
1982 1870 792 778 190
BsmI GAATG_Cn'
Cuts at: 498 2214 498
Size: 1716 3896
Bsp1286I G_dGCh'C
Cuts at: 644 2626 3237 3330 3404 3594 3656 4386 644
Size: 1982 611 93 74 190 62 730 1870
Fragments arranged by size:
1982 1870 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1968 4832 1968
Size: 2864 2748
BspHI T'CATG_A
Cuts at: 4788 4788
Size: 5612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2337 2662 2876 3178 3559 2337
Size: 325 214 302 381 4390
Fragments arranged by size:
4390 381 325 302 214
BsrBI CCG'CTC
Cuts at: 50 3904 3958 5442 5607 50
Size: 3854 54 1484 165 55
Fragments arranged by size:
3854 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 241 805 3524 241
Size: 564 2719 2329
Fragments arranged by size:
2719 2329 564
BsrFI r'CCGG_y
Cuts at: 661 3610 3791 4000 661
Size: 2949 181 209 2273
Fragments arranged by size:
2949 2273 209 181
BsrGI T'GTAC_A
Cuts at: 1532 1532
Size: 5612
BssSI C'ACGA_G
Cuts at: 3883 4241 3883
Size: 358 5254
BstAPI GCAn_nnn'nTGC
Cuts at: 2989 2989
Size: 5612
BstBI TT'CG_AA
Cuts at: 110 3973 110
Size: 3863 1749
BstYI r'GATC_y
Cuts at: 1 158 897 1584 2637 3104 3127 3462 3708
Size: 157 739 687 1053 467 23 335 246
Cuts at: 3708 4720 4806 4818 5164 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1053 1012 739 687 467 449 346 335
246 157 86 23 12
BtgI C'CryG_G
Cuts at: 1391 1391
Size: 5612
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3562 3743 3906 3562
Size: 181 163 5268
Fragments arranged by size:
5268 181 163
BtsI GCAGTG_nn'
Cuts at: 2855 2855
Size: 5612
ClaI AT'CG_AT
Cuts at: 1639 1639
Size: 5612
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 690 725 690
Size: 35 5577
DraI TTT'AAA
Cuts at: 2544 2611 2544
Size: 67 5545
DrdI GACnn_nn'nnGTC
Cuts at: 1724 3318 4176 1724
Size: 1594 858 3160
Fragments arranged by size:
3160 1594 858
EaeI y'GGCC_r
Cuts at: 2223 3197 3371 3762 3789 5134 2223
Size: 974 174 391 27 1345 2701
Fragments arranged by size:
2701 1345 974 391 174 27
EagI C'GGCC_G
Cuts at: 3197 3197
Size: 5612
EarI CTCTTCn'nnn_
Cuts at: 527 1100 1273 1369 2198 2357 3635 3845 527
Size: 573 173 96 829 159 1278 210 2294
Fragments arranged by size:
2294 1278 829 573 210 173 159 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3820 4130 4276 5422 3820
Size: 310 146 1146 4010
Fragments arranged by size:
4010 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2624 2624
Size: 5612
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 905 914 1829 1993 2123 2228 2305 3437
Size: 355 9 915 164 130 105 77 1132
Cuts at: 3437 3869 4616 4884 550
Size: 432 747 268 1278
Fragments arranged by size:
1278 1132 915 747 432 355 268 164
130 105 77 9
EcoO109I rG'GnC_Cy
Cuts at: 2751 2751
Size: 5612
EcoRI G'AATT_C
Cuts at: 463 2616 463
Size: 2153 3459
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1070 2287 3631 1070
Size: 1217 1344 3051
Fragments arranged by size:
3051 1344 1217
FspI TGC'GCA
Cuts at: 3393 3393
Size: 5612
HaeII r_GCGC'y
Cuts at: 3046 3294 4316 5295 3046
Size: 248 1022 979 3363
Fragments arranged by size:
3363 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 1605 1637 1775 1807 2490 2522 3454
Size: 32 1430 32 138 32 683 32 932
Cuts at: 3454 3484 3486 3516 143
Size: 30 2 30 2239
Fragments arranged by size:
2239 1430 932 683 138 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 881 1832 2651 881
Size: 951 819 3842
Fragments arranged by size:
3842 951 819
KasI G'GCGC_C
Cuts at: 3042 3290 3042
Size: 248 5364
KpnI G_GTAC'C
Cuts at: 2632 2632
Size: 5612
MluI A'CGCG_T
Cuts at: 5045 5045
Size: 5612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 951 1753 4283 4467 951
Size: 802 2530 184 2096
Fragments arranged by size:
2530 2096 802 184
MscI TGG'CCA
Cuts at: 2225 3373 2225
Size: 1148 4464
MslI CAynn'nnrTG
Cuts at: 1528 2219 3601 3728 1528
Size: 691 1382 127 3412
Fragments arranged by size:
3412 1382 691 127
MspA1I CmG'CkG
Cuts at: 2723 3037 3397 4410 4655 2723
Size: 314 360 1013 245 3680
Fragments arranged by size:
3680 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3793 3793
Size: 5612
NarI GG'CG_CC
Cuts at: 3043 3291 3043
Size: 248 5364
NcoI C'CATG_G
Cuts at: 1391 1391
Size: 5612
NdeI CA'TA_TG
Cuts at: 615 615
Size: 5612
NgoMIV G'CCGG_C
Cuts at: 3791 3791
Size: 5612
NheI G'CTAG_C
Cuts at: 1909 1909
Size: 5612
NspI r_CATG'y
Cuts at: 192 1921 2665 3165 4072 192
Size: 1729 744 500 907 1732
Fragments arranged by size:
1732 1729 907 744 500
PciI A'CATG_T
Cuts at: 4068 4068
Size: 5612
PmeI GTTT'AAAC
Cuts at: 2611 2611
Size: 5612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4751 4783 4751
Size: 32 5580
PstI C_TGCA'G
Cuts at: 344 996 2659 3344 344
Size: 652 1663 685 2612
Fragments arranged by size:
2612 1663 685 652
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5612
PvuII CAG'CTG
Cuts at: 3037 3397 3037
Size: 360 5252
RsrII CG'GwC_CG
Cuts at: 3807 3807
Size: 5612
SacI G_AGCT'C
Cuts at: 2626 2626
Size: 5612
SalI G'TCGA_C
Cuts at: 2649 2649
Size: 5612
SapI GCTCTTCn'nnn_
Cuts at: 527 3635 3845 527
Size: 3108 210 2294
Fragments arranged by size:
3108 2294 210
SbfI CC_TGCA'GG
Cuts at: 2659 2659
Size: 5612
ScaI AGT'ACT
Cuts at: 1361 5129 1361
Size: 3768 1844
SfcI C'TryA_G
Cuts at: 33 340 992 2655 3340 4333 4524 33
Size: 307 652 1663 685 993 191 1121
Fragments arranged by size:
1663 1121 993 685 652 307 191
SfoI GGC'GCC
Cuts at: 3044 3292 3044
Size: 248 5364
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
SmaI CCC'GGG
Cuts at: 2634 2634
Size: 5612
SmlI C'TyrA_G
Cuts at: 1073 2333 2767 3682 4174 4436 1073
Size: 1260 434 915 492 262 2249
Fragments arranged by size:
2249 1260 915 492 434 262
SnaBI TAC'GTA
Cuts at: 2604 2604
Size: 5612
SpeI A'CTAG_T
Cuts at: 67 777 67
Size: 710 4902
SphI G_CATG'C
Cuts at: 2665 3165 2665
Size: 500 5112
SspI AAT'ATT
Cuts at: 232 603 1660 2072 232
Size: 371 1057 412 3772
Fragments arranged by size:
3772 1057 412 371
StyI C'CwwG_G
Cuts at: 365 1391 2746 3100 365
Size: 1026 1355 354 2877
Fragments arranged by size:
2877 1355 1026 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3195 3328 3787 3996 3195
Size: 133 459 209 4811
Fragments arranged by size:
4811 459 209 133
TatI w'GTAC_w
Cuts at: 238 1359 1492 1532 5127 238
Size: 1121 133 40 3595 723
Fragments arranged by size:
3595 1121 723 133 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1103 1135 2034 2066 5129 5161 1103
Size: 32 899 32 3063 32 1554
Fragments arranged by size:
3063 1554 899 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3409 3409
Size: 5612
XbaI T'CTAG_A
Cuts at: 2643 2643
Size: 5612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3107 3107
Size: 5612
XmaI C'CCGG_G
Cuts at: 2632 2632
Size: 5612
XmnI GAAnn'nnTTC
Cuts at: 563 1803 1964 1988 563
Size: 1240 161 24 4187
Fragments arranged by size:
4187 1240 161 24
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI Bpu10I
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI