Restriction map for
pF1KSDA1293
(Circular) (Six-base) MAPSORT of: pF1KSDA1293.seq Check: 8735 from: 1 to: 4394
pF1KSDA1293 4394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 239 1410 239
Size: 1171 3223
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1201 2219 2651 3398 3666 772
Size: 429 1018 432 747 268 1500
Fragments arranged by size:
1500 1018 747 432 429 268
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1019 1019
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 840 1146 1179 1699 3266 840
Size: 306 33 520 1567 1968
Fragments arranged by size:
1968 1567 520 306 33
ApaLI G'TGCA_C
Cuts at: 232 3164 232
Size: 2932 1462
ApoI r'AATT_y
Cuts at: 339 1398 3747 4003 339
Size: 1059 2349 256 730
Fragments arranged by size:
2349 1059 730 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 191 299 1220 1414 191
Size: 108 921 194 3171
Fragments arranged by size:
3171 921 194 108
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 186 239 275 425 1410 1824 2072 2107 186
Size: 53 36 150 985 414 248 35 2473
Fragments arranged by size:
2473 985 414 248 150 53 36 35
BanII G_rGCy'C
Cuts at: 306 800 1408 2438 306
Size: 494 608 1030 2262
Fragments arranged by size:
2262 1030 608 494
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 153 1286 153
Size: 1133 3261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 290 324 290
Size: 34 4360
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglII A'GATC_T
Cuts at: 148 668 1909 3946 148
Size: 520 1241 2037 596
Fragments arranged by size:
2037 1241 596 520
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 189 236 267 428 2019 2112 3168 189
Size: 47 31 161 1591 93 1056 1415
Fragments arranged by size:
1591 1415 1056 161 93 47 31
BmrI ACTGGGnnnn_n'
Cuts at: 192 238 1073 1606 1687 2022 4166 192
Size: 46 835 533 81 335 2144 420
Fragments arranged by size:
2144 835 533 420 335 81 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 553 1042 1145 490
Size: 63 489 103 3739
Fragments arranged by size:
3739 489 103 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1570 2449 2941 3239 442
Size: 1128 879 492 298 1597
Fragments arranged by size:
1597 1128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 326 4098 326
Size: 3772 622
BsaAI yAC'GTr
Cuts at: 1386 1747 1386
Size: 361 4033
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 303 838 1255 303
Size: 535 417 3442
Fragments arranged by size:
3442 535 417
BseYI C'CCAG_C
Cuts at: 204 1335 1819 2577 3154 3650 204
Size: 1131 484 758 577 496 948
Fragments arranged by size:
1131 948 758 577 496 484
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 236 800 998 1408 2186 2376 3168 236
Size: 564 198 410 778 190 792 1462
Fragments arranged by size:
1462 792 778 564 410 198 190
BsmI GAATG_Cn'
Cuts at: 523 523
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 189 236 267 306 428 800 998 1408 2019
Size: 47 31 39 122 372 198 410 611
Cuts at: 2019 2112 2186 2376 2438 3168 189
Size: 93 74 190 62 730 1415
Fragments arranged by size:
1415 730 611 410 372 198 190 122
93 74 62 47 39 31
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1444 1658 1960 2341 1444
Size: 214 302 381 3497
Fragments arranged by size:
3497 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 873 2306 873
Size: 1433 2961
BsrFI r'CCGG_y
Cuts at: 1087 2392 2573 2782 1087
Size: 1305 181 209 2699
Fragments arranged by size:
2699 1305 209 181
BsrGI T'GTAC_A
Cuts at: 965 965
Size: 4394
BssHII G'CGCG_C
Cuts at: 1357 1357
Size: 4394
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 110 2755 110
Size: 2645 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 969 969
Size: 4394
BstYI r'GATC_y
Cuts at: 1 148 668 873 1013 1175 1248 1419 1886
Size: 147 520 205 140 162 73 171 467
Cuts at: 1886 1909 2244 2490 3502 3588 3600 3946 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 520 467 449 346 335 246 205
171 162 147 140 86 73 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 1265 1637 1265
Size: 372 4022
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 1979 2153 2544 2571 3916 1979
Size: 174 391 27 1345 2457
Fragments arranged by size:
2457 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 805 2417 2627 805
Size: 1612 210 2572
Fragments arranged by size:
2572 1612 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 798 1406 798
Size: 608 3786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 553 772 1042 1145 1201 2219 2651 3398
Size: 63 219 270 103 56 1018 432 747
Cuts at: 3398 3666 490
Size: 268 1218
Fragments arranged by size:
1218 1018 747 432 270 268 219 103
63 56
EcoO109I rG'GnC_Cy
Cuts at: 465 1074 1533 465
Size: 609 459 3326
Fragments arranged by size:
3326 609 459
EcoRI G'AATT_C
Cuts at: 339 1398 339
Size: 1059 3335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1234 1828 2076 3098 4077 1234
Size: 594 248 1022 979 1551
Fragments arranged by size:
1551 1022 979 594 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 801 833 2236 2266 2268 2298 801
Size: 32 1403 30 2 30 2897
Fragments arranged by size:
2897 1403 32 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
HindIII A'AGCT_T
Cuts at: 611 611
Size: 4394
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 243 1414 243
Size: 1171 3223
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3249 3065
Size: 184 4210
MscI TGG'CCA
Cuts at: 2155 2155
Size: 4394
MslI CAynn'nnrTG
Cuts at: 967 1001 2383 2510 967
Size: 34 1382 127 2851
Fragments arranged by size:
2851 1382 127 34
MspA1I CmG'CkG
Cuts at: 140 269 573 1127 1505 1819 2179 3192 3437
Size: 129 304 554 378 314 360 1013 245
Cuts at: 3437 140
Size: 1097
Fragments arranged by size:
1097 1013 554 378 360 314 304 245
129
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 737 1447 1947 2854 737
Size: 710 500 907 2277
Fragments arranged by size:
2277 907 710 500
PasI CC'CwG_GG
Cuts at: 861 861
Size: 4394
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 1299 1299
Size: 4394
PfoI T'CCnGG_A
Cuts at: 467 527 725 1109 467
Size: 60 198 384 3752
Fragments arranged by size:
3752 384 198 60
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 465 1074 465
Size: 609 3785
PspXI vC'TCGA_Gb
Cuts at: 299 299
Size: 4394
PstI C_TGCA'G
Cuts at: 809 960 1441 2126 809
Size: 151 481 685 3077
Fragments arranged by size:
3077 685 481 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1127 1819 2179 1127
Size: 692 360 3342
Fragments arranged by size:
3342 692 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 800 1408 800
Size: 608 3786
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SanDI GG'GwC_CC
Cuts at: 1074 1074
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 805 2417 2627 805
Size: 1612 210 2572
Fragments arranged by size:
2572 1612 210
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 805 950 956 1437 2122 3115 3306 33
Size: 772 145 6 481 685 993 191 1121
Fragments arranged by size:
1121 993 772 685 481 191 145 6
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 193 1222 1416 193
Size: 1029 194 3171
Fragments arranged by size:
3171 1029 194
SmlI C'TyrA_G
Cuts at: 299 457 1549 2464 2956 3218 299
Size: 158 1092 915 492 262 1475
Fragments arranged by size:
1475 1092 915 492 262 158
SnaBI TAC'GTA
Cuts at: 1386 1386
Size: 4394
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 544 1528 1882 544
Size: 984 354 3056
Fragments arranged by size:
3056 984 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 443 1977 2110 2569 2778 443
Size: 1534 133 459 209 2059
Fragments arranged by size:
2059 1534 459 209 133
TatI w'GTAC_w
Cuts at: 473 917 965 3909 473
Size: 444 48 2944 958
Fragments arranged by size:
2944 958 444 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 179 251 1889 179
Size: 72 1638 2684
Fragments arranged by size:
2684 1638 72
XhoI C'TCGA_G
Cuts at: 299 299
Size: 4394
XmaI C'CCGG_G
Cuts at: 191 1220 1414 191
Size: 1029 194 3171
Fragments arranged by size:
3171 1029 194
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PsrI SacII SexAI
SfiI SgrAI SrfI SspI StuI SwaI XmnI ZraI