Restriction map for
pF1KSDA1281
(Circular) (Six-base) MAPSORT of: pF1KSDA1281.seq Check: 2492 from: 1 to: 7673
pF1KSDA1281 7673 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4511 4599 4511
Size: 88 7585
AccI GT'mk_AC
Cuts at: 3806 3863 4711 3806
Size: 57 848 6768
Fragments arranged by size:
6768 848 57
Acc65I G'GTAC_C
Cuts at: 1838 4689 1838
Size: 2851 4822
AclI AA'CG_TT
Cuts at: 7513 7513
Size: 7673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 3688 5498 5930 6677 6945 763
Size: 2925 1810 432 747 268 1491
Fragments arranged by size:
2925 1810 1491 747 432 268
AfeI AGC'GCT
Cuts at: 581 581
Size: 7673
AflIII A'CryG_T
Cuts at: 1186 5025 6129 7106 1186
Size: 3839 1104 977 1753
Fragments arranged by size:
3839 1753 1104 977
AgeI A'CCGG_T
Cuts at: 1140 6061 1140
Size: 4921 2752
AhdI GACnn_n'nnGTC
Cuts at: 2985 3399 2985
Size: 414 7259
AleI CACnn'nnGTG
Cuts at: 2851 2851
Size: 7673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1965 3185 4631 1965
Size: 1220 1446 5007
Fragments arranged by size:
5007 1446 1220
AlwNI CAG_nnn'CTG
Cuts at: 3465 4978 6545 3465
Size: 1513 1567 4593
Fragments arranged by size:
4593 1567 1513
ApaI G_GGCC'C
Cuts at: 1229 1368 1229
Size: 139 7534
ApaLI G'TGCA_C
Cuts at: 6443 6443
Size: 7673
ApoI r'AATT_y
Cuts at: 219 265 376 411 573 1251 1701 2100 2253
Size: 46 111 35 162 678 450 399 153
Cuts at: 2253 4677 7026 7282 219
Size: 2424 2349 256 610
Fragments arranged by size:
2424 2349 678 610 450 399 256 162
153 111 46 35
AseI AT'TA_AT
Cuts at: 20 2239 20
Size: 2219 5454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7673
AvaI C'yCGr_G
Cuts at: 508 1458 2829 3816 4100 4551 4693 508
Size: 950 1371 987 284 451 142 3488
Fragments arranged by size:
3488 1371 987 950 451 284 142
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1955 1988 1955
Size: 33 7640
BamHI G'GATC_C
Cuts at: 162 4698 162
Size: 4536 3137
BanI G'GyrC_C
Cuts at: 548 724 824 1838 1995 2865 2874 3169 4499
Size: 176 100 1014 157 870 9 295 1330
Cuts at: 4499 4689 5103 5351 5386 548
Size: 190 414 248 35 2835
Fragments arranged by size:
2835 1330 1014 870 414 295 248 190
176 157 100 35 9
BanII G_rGCy'C
Cuts at: 871 950 1229 1368 1465 2519 3386 3524 4010
Size: 79 279 139 97 1054 867 138 486
Cuts at: 4010 4687 5717 871
Size: 677 1030 2827
Fragments arranged by size:
2827 1054 1030 867 677 486 279 139
138 97 79
BbeI G_GCGC'C
Cuts at: 552 728 5107 5355 552
Size: 176 4379 248 2870
Fragments arranged by size:
4379 2870 248 176
BbvCI CC'TCA_GC
Cuts at: 1586 2768 3308 1586
Size: 1182 540 5951
Fragments arranged by size:
5951 1182 540
BciVI GTATCCnnnnn_n'
Cuts at: 5571 6332 7673 5571
Size: 761 1341 5571
Fragments arranged by size:
5571 1341 761
BclI T'GATC_A
Cuts at: 3323 5193 3323
Size: 1870 5803
BfrBI ATG'CAT
Cuts at: 3376 3376
Size: 7673
BglII A'GATC_T
Cuts at: 1907 5188 7225 1907
Size: 3281 2037 2355
Fragments arranged by size:
3281 2355 2037
BlpI GC'TnA_GC
Cuts at: 3725 4442 4785 3725
Size: 717 343 6613
Fragments arranged by size:
6613 717 343
Bme1580I G_kGCm'C
Cuts at: 827 889 936 1229 1368 2331 3174 3971 5298
Size: 62 47 293 139 963 843 797 1327
Cuts at: 5298 5391 6447 827
Size: 93 1056 2053
Fragments arranged by size:
2053 1327 1056 963 843 797 293 139
93 62 47
BmrI ACTGGGnnnn_n'
Cuts at: 899 1371 2420 3500 4885 4966 5301 7445 899
Size: 472 1049 1080 1385 81 335 2144 1127
Fragments arranged by size:
2144 1385 1127 1080 1049 472 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 6040 3962
Size: 2078 5595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 575 1423 1644 1757 1900 4247 4366 318
Size: 257 848 221 113 143 2347 119 3625
Fragments arranged by size:
3625 2347 848 257 221 143 119 113
Bpu10I CC'TnA_GC
Cuts at: 360 1586 2768 3308 360
Size: 1226 1182 540 4725
Fragments arranged by size:
4725 1226 1182 540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 4849 5728 6220 6518 1712
Size: 3137 879 492 298 2867
Fragments arranged by size:
3137 2867 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 7377 418
Size: 6959 714
BsaAI yAC'GTr
Cuts at: 1310 1329 4665 5026 1310
Size: 19 3336 361 3957
Fragments arranged by size:
3957 3336 361 19
BsaBI GATnn'nnATC
Cuts at: 6 5187 7224 6
Size: 5181 2037 455
Fragments arranged by size:
5181 2037 455
BsaHI Gr'CG_yC
Cuts at: 549 725 5104 5352 6054 7550 549
Size: 176 4379 248 702 1496 672
Fragments arranged by size:
4379 1496 702 672 248 176
BsaWI w'CCGG_w
Cuts at: 1140 3603 4862 5383 6061 6335 6482 6893 1140
Size: 2463 1259 521 678 274 147 411 1920
Fragments arranged by size:
2463 1920 1259 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1642 1672 1642
Size: 30 7643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 997 1000 1003 1006 3512 3673 4018 4060
Size: 291 3 3 3 2506 161 345 42
Cuts at: 4060 4113 706
Size: 53 4266
Fragments arranged by size:
4266 2506 345 291 161 53 42 3
3 3
BseYI C'CCAG_C
Cuts at: 582 2092 2349 2532 2631 2806 3537 3970 4195
Size: 1510 257 183 99 175 731 433 225
Cuts at: 4195 5098 5856 6433 6929 582
Size: 903 758 577 496 1326
Fragments arranged by size:
1510 1326 903 758 731 577 496 433
257 225 183 175 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1332 4489 1332
Size: 3157 4516
BsiEI CG_ry'CG
Cuts at: 104 5215 5261 6469 104
Size: 5111 46 1208 1308
Fragments arranged by size:
5111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3199 3524 4687 5465 5655 6447 3199
Size: 325 1163 778 190 792 4425
Fragments arranged by size:
4425 1163 792 778 325 190
BsiWI C'GTAC_G
Cuts at: 1325 1325
Size: 7673
BsmI GAATG_Cn'
Cuts at: 1954 2845 3060 1954
Size: 891 215 6567
Fragments arranged by size:
6567 891 215
BsmBI CGTCTCn'nnnn_
Cuts at: 2596 2596
Size: 7673
Bsp1286I G_dGCh'C
Cuts at: 827 871 889 936 950 1229 1368 1465 2331
Size: 44 18 47 14 279 139 97 866
Cuts at: 2331 2519 3174 3199 3386 3524 3971 4010 4687
Size: 188 655 25 187 138 447 39 677
Cuts at: 4687 5298 5391 5465 5655 5717 6447 827
Size: 611 93 74 190 62 730 2053
Fragments arranged by size:
2053 866 730 677 655 611 447 279
190 188 187 139 138 97 93 74
62 47 44 39 25 18 14
BspEI T'CCGG_A
Cuts at: 3603 6893 3603
Size: 3290 4383
BspHI T'CATG_A
Cuts at: 6849 6849
Size: 7673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 782 2975 3153 4511 4599 4723 4937 5239 5620
Size: 2193 178 1358 88 124 214 302 381
Cuts at: 5620 782
Size: 2835
Fragments arranged by size:
2835 2193 1358 381 302 214 178 124
88
BsrBI CCG'CTC
Cuts at: 50 4446 5965 6019 7503 7668 50
Size: 4396 1519 54 1484 165 55
Fragments arranged by size:
4396 1519 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 858 2067 2336 5585 858
Size: 1209 269 3249 2946
Fragments arranged by size:
3249 2946 1209 269
BsrFI r'CCGG_y
Cuts at: 1140 1998 5671 5852 6061 1140
Size: 858 3673 181 209 2752
Fragments arranged by size:
3673 2752 858 209 181
BssSI C'ACGA_G
Cuts at: 5944 6302 5944
Size: 358 7315
BstAPI GCAn_nnn'nTGC
Cuts at: 774 1444 5050 774
Size: 670 3606 3397
Fragments arranged by size:
3606 3397 670
BstBI TT'CG_AA
Cuts at: 110 6034 110
Size: 5924 1749
BstEII G'GTnAC_C
Cuts at: 4537 4537
Size: 7673
BstXI CCAn_nnnn'nTGG
Cuts at: 793 2508 3879 793
Size: 1715 1371 4587
Fragments arranged by size:
4587 1715 1371
BstYI r'GATC_y
Cuts at: 1 162 1907 4698 5165 5188 5523 5769 6781
Size: 161 1745 2791 467 23 335 246 1012
Cuts at: 6781 6867 6879 7225 1
Size: 86 12 346 449
Fragments arranged by size:
2791 1745 1012 467 449 346 335 246
161 86 23 12
BstZ17I GTA'TAC
Cuts at: 3864 3864
Size: 7673
Bsu36I CC'TnA_GG
Cuts at: 1834 1834
Size: 7673
BtgI C'CryG_G
Cuts at: 659 659
Size: 7673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 209 5623 5804 5967 209
Size: 5414 181 163 1915
Fragments arranged by size:
5414 1915 181 163
BtsI GCAGTG_nn'
Cuts at: 804 876 1959 2384 4916 804
Size: 72 1083 425 2532 3561
Fragments arranged by size:
3561 2532 1083 425 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 300 335 840 875 300
Size: 35 505 35 7098
Fragments arranged by size:
7098 505 35 35
DraI TTT'AAA
Cuts at: 2687 3825 4672 2687
Size: 1138 847 5688
Fragments arranged by size:
5688 1138 847
DrdI GACnn_nn'nnGTC
Cuts at: 5379 6237 5379
Size: 858 6815
EaeI y'GGCC_r
Cuts at: 2753 3366 3410 3575 4491 5258 5432 5823 5850
Size: 613 44 165 916 767 174 391 27
Cuts at: 5850 7195 2753
Size: 1345 3231
Fragments arranged by size:
3231 1345 916 767 613 391 174 165
44 27
EagI C'GGCC_G
Cuts at: 5258 5258
Size: 7673
EarI CTCTTCn'nnn_
Cuts at: 974 1426 1773 4301 4650 5696 5906 974
Size: 452 347 2528 349 1046 210 2741
Fragments arranged by size:
2741 2528 1046 452 349 347 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5881 6191 6337 7483 5881
Size: 310 146 1146 6071
Fragments arranged by size:
6071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3522 4685 3522
Size: 1163 6510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 575 763 1423 1644 1757 1900 3688 4247
Size: 257 188 660 221 113 143 1788 559
Cuts at: 4247 4366 5498 5930 6677 6945 318
Size: 119 1132 432 747 268 1046
Fragments arranged by size:
1788 1132 1046 747 660 559 432 268
257 221 188 143 119 113
EcoNI CCTnn'n_nnAGG
Cuts at: 778 2816 4571 778
Size: 2038 1755 3880
Fragments arranged by size:
3880 2038 1755
EcoO109I rG'GnC_Cy
Cuts at: 810 1226 1365 2030 2118 4812 810
Size: 416 139 665 88 2694 3671
Fragments arranged by size:
3671 2694 665 416 139 88
EcoRI G'AATT_C
Cuts at: 411 573 2100 4677 411
Size: 162 1527 2577 3407
Fragments arranged by size:
3407 2577 1527 162
EcoRV GAT'ATC
Cuts at: 1593 1593
Size: 7673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5692 5692
Size: 7673
FspI TGC'GCA
Cuts at: 5454 5454
Size: 7673
HaeII r_GCGC'y
Cuts at: 552 583 728 1060 2828 5107 5355 6377 7356
Size: 31 145 332 1768 2279 248 1022 979
Cuts at: 7356 552
Size: 869
Fragments arranged by size:
2279 1768 1022 979 869 332 248 145
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1642 1674 1894 1926 2167 2199 2504 2536 2971
Size: 32 220 32 241 32 305 32 435
Cuts at: 2971 3003 3385 3417 3799 3831 4021 4053 4103
Size: 32 382 32 382 32 190 32 50
Cuts at: 4103 4125 4135 4157 4465 4497 5515 5545 5547
Size: 22 10 22 308 32 1018 30 2
Cuts at: 5547 5577 1642
Size: 30 3738
Fragments arranged by size:
3738 1018 435 382 382 308 305 241
220 190 50 32 32 32 32 32
32 32 32 32 30 30 22 22
10 2
HincII GTy'rAC
Cuts at: 1035 2891 3989 4341 4712 1035
Size: 1856 1098 352 371 3996
Fragments arranged by size:
3996 1856 1098 371 352
HpaI GTT'AAC
Cuts at: 3989 3989
Size: 7673
KasI G'GCGC_C
Cuts at: 548 724 5103 5351 548
Size: 176 4379 248 2870
Fragments arranged by size:
4379 2870 248 176
KpnI G_GTAC'C
Cuts at: 1842 4693 1842
Size: 2851 4822
MluI A'CGCG_T
Cuts at: 7106 7106
Size: 7673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 195 1176 2180 2711 2969 6344 6528 195
Size: 981 1004 531 258 3375 184 1340
Fragments arranged by size:
3375 1340 1004 981 531 258 184
MscI TGG'CCA
Cuts at: 2755 3368 3412 5434 2755
Size: 613 44 2022 4994
Fragments arranged by size:
4994 2022 613 44
MslI CAynn'nnrTG
Cuts at: 130 791 830 1947 2506 2524 2851 3122 3857
Size: 661 39 1117 559 18 327 271 735
Cuts at: 3857 5662 5789 130
Size: 1805 127 2014
Fragments arranged by size:
2014 1805 1117 735 661 559 327 271
127 39 18
MspA1I CmG'CkG
Cuts at: 602 611 661 910 1556 2917 3181 4441 4497
Size: 9 50 249 646 1361 264 1260 56
Cuts at: 4497 4784 5098 5458 6471 6716 602
Size: 287 314 360 1013 245 1559
Fragments arranged by size:
1559 1361 1260 1013 646 360 314 287
264 249 245 56 50 9
NaeI GCC'GGC
Cuts at: 5854 5854
Size: 7673
NarI GG'CG_CC
Cuts at: 549 725 5104 5352 549
Size: 176 4379 248 2870
Fragments arranged by size:
4379 2870 248 176
NdeI CA'TA_TG
Cuts at: 3378 3378
Size: 7673
NgoMIV G'CCGG_C
Cuts at: 5852 5852
Size: 7673
NsiI A_TGCA'T
Cuts at: 3378 3378
Size: 7673
NspI r_CATG'y
Cuts at: 1190 2887 3153 4168 4393 4726 5226 6133 1190
Size: 1697 266 1015 225 333 500 907 2730
Fragments arranged by size:
2730 1697 1015 907 500 333 266 225
PasI CC'CwG_GG
Cuts at: 405 3157 405
Size: 2752 4921
PciI A'CATG_T
Cuts at: 1186 6129 1186
Size: 4943 2730
PflMI CCAn_nnn'nTGG
Cuts at: 3465 3465
Size: 7673
PfoI T'CCnGG_A
Cuts at: 754 4263 4644 754
Size: 3509 381 3783
Fragments arranged by size:
3783 3509 381
PmeI GTTT'AAAC
Cuts at: 4672 4672
Size: 7673
PmlI CAC'GTG
Cuts at: 1310 1310
Size: 7673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1648 1680 6812 6844 1648
Size: 32 5132 32 2477
Fragments arranged by size:
5132 2477 32 32
PpuMI rG'GwC_Cy
Cuts at: 2030 2030
Size: 7673
PspOMI G'GGCC_C
Cuts at: 1225 1364 1225
Size: 139 7534
PspXI vC'TCGA_Gb
Cuts at: 2829 2829
Size: 7673
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1309 1341 1309
Size: 32 7641
PstI C_TGCA'G
Cuts at: 150 915 1499 3180 4720 5405 150
Size: 765 584 1681 1540 685 2418
Fragments arranged by size:
2418 1681 1540 765 685 584
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7673
PvuII CAG'CTG
Cuts at: 2917 3181 4441 5098 5458 2917
Size: 264 1260 657 360 5132
Fragments arranged by size:
5132 1260 657 360 264
RsrII CG'GwC_CG
Cuts at: 5868 5868
Size: 7673
SacI G_AGCT'C
Cuts at: 3524 4687 3524
Size: 1163 6510
SacII CC_GC'GG
Cuts at: 662 662
Size: 7673
SalI G'TCGA_C
Cuts at: 4710 4710
Size: 7673
SanDI GG'GwC_CC
Cuts at: 2030 2030
Size: 7673
SapI GCTCTTCn'nnn_
Cuts at: 1773 5696 5906 1773
Size: 3923 210 3540
Fragments arranged by size:
3923 3540 210
SbfI CC_TGCA'GG
Cuts at: 4720 4720
Size: 7673
ScaI AGT'ACT
Cuts at: 3448 4159 7190 3448
Size: 711 3031 3931
Fragments arranged by size:
3931 3031 711
SfcI C'TryA_G
Cuts at: 33 146 911 1495 3071 3176 3426 3761 4436
Size: 113 765 584 1576 105 250 335 675
Cuts at: 4436 4716 5401 6394 6585 33
Size: 280 685 993 191 1121
Fragments arranged by size:
1576 1121 993 765 685 675 584 335
280 250 191 113 105
SfoI GGC'GCC
Cuts at: 550 726 5105 5353 550
Size: 176 4379 248 2870
Fragments arranged by size:
4379 2870 248 176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7673
SmaI CCC'GGG
Cuts at: 1460 4553 4695 1460
Size: 3093 142 4438
Fragments arranged by size:
4438 3093 142
SmlI C'TyrA_G
Cuts at: 1727 2829 3816 4828 5743 6235 6497 1727
Size: 1102 987 1012 915 492 262 2903
Fragments arranged by size:
2903 1102 1012 987 915 492 262
SnaBI TAC'GTA
Cuts at: 4665 4665
Size: 7673
SpeI A'CTAG_T
Cuts at: 67 295 67
Size: 228 7445
SphI G_CATG'C
Cuts at: 4168 4393 4726 5226 4168
Size: 225 333 500 6615
Fragments arranged by size:
6615 500 333 225
SspI AAT'ATT
Cuts at: 1131 1131
Size: 7673
StuI AGG'CCT
Cuts at: 394 394
Size: 7673
StyI C'CwwG_G
Cuts at: 311 543 1758 2651 4807 5161 311
Size: 232 1215 893 2156 354 2823
Fragments arranged by size:
2823 2156 1215 893 354 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1165 1811 4143 4537 5256 5389 5848 6057 1165
Size: 646 2332 394 719 133 459 209 2781
Fragments arranged by size:
2781 2332 719 646 459 394 209 133
TatI w'GTAC_w
Cuts at: 1814 3446 4157 7188 1814
Size: 1632 711 3031 2299
Fragments arranged by size:
3031 2299 1632 711
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 143 175 4097 4129 7190 7222 143
Size: 32 3922 32 3061 32 594
Fragments arranged by size:
3922 3061 594 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5470 5470
Size: 7673
XbaI T'CTAG_A
Cuts at: 4704 4704
Size: 7673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 550 796 1251 3376 4525 5168 550
Size: 246 455 2125 1149 643 3055
Fragments arranged by size:
3055 2125 1149 643 455 246
XhoI C'TCGA_G
Cuts at: 2829 3816 2829
Size: 987 6686
XmaI C'CCGG_G
Cuts at: 1458 4551 4693 1458
Size: 3093 142 4438
Fragments arranged by size:
4438 3093 142
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BbsI BcgI BglI
BmgBI BmtI BsrGI BssHII ClaI DraIII FseI FspAI
HindIII MfeI NcoI NheI NotI NruI PacI PshAI
PsiI SexAI SfiI SgrAI SrfI SwaI XmnI ZraI